Incidental Mutation 'R7202:Nek10'
| ID |
560398 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Nek10
|
| Ensembl Gene |
ENSMUSG00000042567 |
| Gene Name |
NIMA (never in mitosis gene a)- related kinase 10 |
| Synonyms |
LOC238944 |
| MMRRC Submission |
045280-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R7202 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
14 |
| Chromosomal Location |
7457704-7666183 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
C to A
at 14836171 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Histidine to Asparagine
at position 131
(H131N)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000108249
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000112630]
[ENSMUST00000112631]
[ENSMUST00000224491]
|
| AlphaFold |
Q3UGM2 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000112630
AA Change: H131N
PolyPhen 2
Score 0.010 (Sensitivity: 0.96; Specificity: 0.77)
|
| SMART Domains |
Protein: ENSMUSP00000108249
Gene: ENSMUSG00000042567
AA Change: H131N
| Domain | Start | End | E-Value | Type |
|---|
|
ARM
|
197 |
238 |
8.23e1 |
SMART |
|
ARM
|
278 |
320 |
5.18e0 |
SMART |
|
low complexity region
|
387 |
400 |
N/A |
INTRINSIC |
|
ARM
|
401 |
448 |
7.09e1 |
SMART |
|
S_TKc
|
519 |
791 |
2.36e-75 |
SMART |
|
low complexity region
|
799 |
811 |
N/A |
INTRINSIC |
|
low complexity region
|
839 |
863 |
N/A |
INTRINSIC |
|
low complexity region
|
908 |
926 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000112631
AA Change: H131N
PolyPhen 2
Score 0.010 (Sensitivity: 0.96; Specificity: 0.77)
|
| SMART Domains |
Protein: ENSMUSP00000108250
Gene: ENSMUSG00000042567
AA Change: H131N
| Domain | Start | End | E-Value | Type |
|---|
|
ARM
|
197 |
238 |
8.23e1 |
SMART |
|
ARM
|
278 |
320 |
5.18e0 |
SMART |
|
low complexity region
|
387 |
400 |
N/A |
INTRINSIC |
|
ARM
|
401 |
448 |
7.09e1 |
SMART |
|
S_TKc
|
519 |
791 |
2.36e-75 |
SMART |
|
low complexity region
|
799 |
811 |
N/A |
INTRINSIC |
|
low complexity region
|
839 |
863 |
N/A |
INTRINSIC |
|
low complexity region
|
908 |
926 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000224491
AA Change: H131N
PolyPhen 2
Score 0.010 (Sensitivity: 0.96; Specificity: 0.77)
|
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 100.0%
- 10x: 99.7%
- 20x: 99.0%
|
| Validation Efficiency |
99% (74/75) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 74 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Abca14 |
T |
C |
7: 119,917,236 (GRCm39) |
I1467T |
probably damaging |
Het |
| Abtb3 |
A |
G |
10: 85,223,629 (GRCm39) |
E146G |
unknown |
Het |
| Adgrf3 |
T |
A |
5: 30,409,378 (GRCm39) |
K38* |
probably null |
Het |
| Ahdc1 |
T |
A |
4: 132,789,198 (GRCm39) |
C146* |
probably null |
Het |
| Ahnak |
A |
T |
19: 8,995,163 (GRCm39) |
K5482N |
probably damaging |
Het |
| Arhgap23 |
C |
T |
11: 97,342,819 (GRCm39) |
A367V |
possibly damaging |
Het |
| Arvcf |
T |
C |
16: 18,223,948 (GRCm39) |
S960P |
probably damaging |
Het |
| Asb14 |
C |
A |
14: 26,622,394 (GRCm39) |
P74Q |
probably benign |
Het |
| Atad5 |
T |
C |
11: 79,980,601 (GRCm39) |
V2A |
probably damaging |
Het |
| Baz2a |
A |
G |
10: 127,954,428 (GRCm39) |
I691M |
possibly damaging |
Het |
| Brca2 |
A |
G |
5: 150,455,819 (GRCm39) |
T187A |
probably benign |
Het |
| Calr4 |
T |
A |
4: 109,101,254 (GRCm39) |
N152K |
possibly damaging |
Het |
| Ccnb2 |
C |
T |
9: 70,318,128 (GRCm39) |
A215T |
probably damaging |
Het |
| Cdh16 |
C |
T |
8: 105,340,780 (GRCm39) |
D140N |
unknown |
Het |
| Ces1g |
T |
C |
8: 94,029,595 (GRCm39) |
T546A |
probably benign |
Het |
| Cfap74 |
G |
T |
4: 155,510,654 (GRCm39) |
|
probably null |
Het |
| Cfap91 |
T |
C |
16: 38,155,959 (GRCm39) |
Y88C |
probably benign |
Het |
| Col5a1 |
G |
T |
2: 27,842,390 (GRCm39) |
D422Y |
unknown |
Het |
| Cyp2c66 |
A |
T |
19: 39,130,348 (GRCm39) |
Y61F |
probably damaging |
Het |
| Dnah12 |
G |
A |
14: 26,500,869 (GRCm39) |
|
probably null |
Het |
| Dnah6 |
A |
G |
6: 73,158,688 (GRCm39) |
|
probably null |
Het |
| Dop1a |
T |
G |
9: 86,386,220 (GRCm39) |
|
probably null |
Het |
| Fam163a |
A |
G |
1: 155,954,834 (GRCm39) |
F106L |
probably damaging |
Het |
| Gfy |
T |
C |
7: 44,827,596 (GRCm39) |
T167A |
probably benign |
Het |
| Gm6525 |
A |
T |
3: 84,082,341 (GRCm39) |
R87S |
probably benign |
Het |
| Gramd1c |
C |
A |
16: 43,879,584 (GRCm39) |
A19S |
possibly damaging |
Het |
| Herc2 |
T |
C |
7: 55,781,034 (GRCm39) |
V1233A |
probably damaging |
Het |
| Hibch |
G |
A |
1: 52,892,874 (GRCm39) |
|
probably null |
Het |
| Ibsp |
A |
T |
5: 104,450,027 (GRCm39) |
S9C |
probably benign |
Het |
| Il17ra |
A |
G |
6: 120,452,572 (GRCm39) |
I252V |
probably benign |
Het |
| Ino80 |
A |
T |
2: 119,204,918 (GRCm39) |
M1536K |
probably benign |
Het |
| Ipo11 |
A |
T |
13: 107,012,078 (GRCm39) |
L568Q |
probably damaging |
Het |
| Kcnu1 |
T |
G |
8: 26,409,609 (GRCm39) |
|
probably null |
Het |
| Kdm5b |
T |
G |
1: 134,552,497 (GRCm39) |
S1158A |
probably benign |
Het |
| Kmt2a |
T |
C |
9: 44,759,012 (GRCm39) |
T946A |
probably benign |
Het |
| Kmt2e |
A |
T |
5: 23,697,292 (GRCm39) |
|
probably benign |
Het |
| Krba1 |
G |
A |
6: 48,389,261 (GRCm39) |
V594M |
probably damaging |
Het |
| Lamb1 |
T |
C |
12: 31,374,314 (GRCm39) |
L1559P |
probably damaging |
Het |
| Lig1 |
C |
T |
7: 13,025,175 (GRCm39) |
P227S |
probably benign |
Het |
| Lims2 |
T |
A |
18: 32,090,017 (GRCm39) |
D255E |
probably benign |
Het |
| Marchf3 |
C |
T |
18: 56,909,161 (GRCm39) |
C208Y |
probably benign |
Het |
| Mei1 |
A |
G |
15: 81,976,843 (GRCm39) |
E142G |
|
Het |
| Melk |
G |
T |
4: 44,351,106 (GRCm39) |
R549L |
probably benign |
Het |
| Muc6 |
AGGCGCAGAAACCCTGGC |
AGGC |
7: 141,214,363 (GRCm39) |
|
probably null |
Het |
| Myorg |
T |
C |
4: 41,498,268 (GRCm39) |
Y454C |
probably damaging |
Het |
| Nae1 |
T |
C |
8: 105,250,215 (GRCm39) |
Y226C |
possibly damaging |
Het |
| Nap1l1 |
T |
C |
10: 111,326,964 (GRCm39) |
F175S |
probably damaging |
Het |
| Ndn |
T |
C |
7: 61,998,709 (GRCm39) |
L185P |
probably damaging |
Het |
| Ndst3 |
T |
A |
3: 123,465,388 (GRCm39) |
I195F |
possibly damaging |
Het |
| Ndufaf1 |
A |
T |
2: 119,488,907 (GRCm39) |
S206T |
probably benign |
Het |
| Obsl1 |
T |
A |
1: 75,466,360 (GRCm39) |
D1456V |
possibly damaging |
Het |
| Or10a2 |
A |
G |
7: 106,673,448 (GRCm39) |
N138D |
probably benign |
Het |
| Or10ak7 |
C |
T |
4: 118,791,215 (GRCm39) |
V277I |
probably benign |
Het |
| Or52s6 |
T |
A |
7: 103,092,292 (GRCm39) |
T13S |
probably benign |
Het |
| Otog |
C |
T |
7: 45,937,474 (GRCm39) |
L1728F |
probably damaging |
Het |
| Pcx |
A |
T |
19: 4,652,361 (GRCm39) |
M150L |
possibly damaging |
Het |
| Pole |
A |
G |
5: 110,444,973 (GRCm39) |
D443G |
possibly damaging |
Het |
| Prr14 |
G |
T |
7: 127,075,648 (GRCm39) |
R552L |
probably damaging |
Het |
| Rasgrf1 |
T |
G |
9: 89,899,125 (GRCm39) |
S1156A |
possibly damaging |
Het |
| Rfc4 |
T |
A |
16: 22,946,359 (GRCm39) |
|
probably benign |
Het |
| Rfx5 |
G |
T |
3: 94,866,272 (GRCm39) |
A524S |
unknown |
Het |
| Ryr3 |
G |
A |
2: 112,596,664 (GRCm39) |
P2497S |
probably damaging |
Het |
| Scg3 |
T |
C |
9: 75,590,992 (GRCm39) |
T6A |
probably benign |
Het |
| Slc26a9 |
A |
T |
1: 131,690,526 (GRCm39) |
D510V |
possibly damaging |
Het |
| Stpg3 |
A |
G |
2: 25,104,586 (GRCm39) |
F10L |
probably damaging |
Het |
| Tmc5 |
T |
A |
7: 118,239,179 (GRCm39) |
H357Q |
possibly damaging |
Het |
| Tmem40 |
A |
T |
6: 115,707,420 (GRCm39) |
L253H |
probably damaging |
Het |
| Tmie |
A |
G |
9: 110,696,632 (GRCm39) |
V83A |
probably damaging |
Het |
| Uck2 |
A |
T |
1: 167,054,084 (GRCm39) |
I218N |
probably damaging |
Het |
| Vtn |
A |
G |
11: 78,391,626 (GRCm39) |
D310G |
possibly damaging |
Het |
| Wdr49 |
A |
T |
3: 75,240,580 (GRCm39) |
S430T |
probably benign |
Het |
| Wtip |
A |
G |
7: 33,832,087 (GRCm39) |
V133A |
probably benign |
Het |
| Zbtb9 |
T |
A |
17: 27,193,666 (GRCm39) |
V357E |
probably damaging |
Het |
| Zfp953 |
G |
A |
13: 67,491,706 (GRCm39) |
T82M |
probably benign |
Het |
|
| Other mutations in Nek10 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01361:Nek10
|
APN |
14 |
14,850,957 (GRCm38) |
missense |
probably damaging |
0.99 |
|
IGL02067:Nek10
|
APN |
14 |
14,861,639 (GRCm38) |
missense |
probably benign |
0.12 |
|
IGL02361:Nek10
|
APN |
14 |
14,843,856 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL02687:Nek10
|
APN |
14 |
14,840,570 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL02929:Nek10
|
APN |
14 |
14,821,119 (GRCm38) |
missense |
possibly damaging |
0.82 |
|
IGL03229:Nek10
|
APN |
14 |
14,986,686 (GRCm38) |
missense |
probably benign |
0.10 |
|
P0041:Nek10
|
UTSW |
14 |
14,861,603 (GRCm38) |
missense |
probably benign |
0.01 |
|
R0007:Nek10
|
UTSW |
14 |
14,840,574 (GRCm38) |
missense |
probably benign |
0.10 |
|
R0007:Nek10
|
UTSW |
14 |
14,840,574 (GRCm38) |
missense |
probably benign |
0.10 |
|
R0142:Nek10
|
UTSW |
14 |
14,861,560 (GRCm38) |
missense |
possibly damaging |
0.96 |
|
R0433:Nek10
|
UTSW |
14 |
14,860,927 (GRCm38) |
missense |
probably benign |
0.32 |
|
R0633:Nek10
|
UTSW |
14 |
14,857,782 (GRCm38) |
critical splice acceptor site |
probably null |
|
|
R1087:Nek10
|
UTSW |
14 |
14,827,059 (GRCm38) |
missense |
possibly damaging |
0.59 |
|
R1184:Nek10
|
UTSW |
14 |
14,931,325 (GRCm38) |
splice site |
probably benign |
|
|
R1250:Nek10
|
UTSW |
14 |
14,853,887 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R1371:Nek10
|
UTSW |
14 |
14,850,983 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R1506:Nek10
|
UTSW |
14 |
14,999,078 (GRCm38) |
splice site |
probably benign |
|
|
R1829:Nek10
|
UTSW |
14 |
14,863,454 (GRCm38) |
critical splice acceptor site |
probably null |
|
|
R1831:Nek10
|
UTSW |
14 |
14,842,789 (GRCm38) |
missense |
probably benign |
|
|
R1833:Nek10
|
UTSW |
14 |
14,842,789 (GRCm38) |
missense |
probably benign |
|
|
R1990:Nek10
|
UTSW |
14 |
14,860,764 (GRCm38) |
missense |
probably benign |
|
|
R1997:Nek10
|
UTSW |
14 |
14,827,003 (GRCm38) |
missense |
probably benign |
0.09 |
|
R2011:Nek10
|
UTSW |
14 |
14,885,122 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R2158:Nek10
|
UTSW |
14 |
14,885,047 (GRCm38) |
splice site |
probably null |
|
|
R2288:Nek10
|
UTSW |
14 |
14,853,956 (GRCm38) |
nonsense |
probably null |
|
|
R2568:Nek10
|
UTSW |
14 |
14,999,112 (GRCm38) |
missense |
possibly damaging |
0.89 |
|
R2907:Nek10
|
UTSW |
14 |
14,980,613 (GRCm38) |
missense |
possibly damaging |
0.81 |
|
R2965:Nek10
|
UTSW |
14 |
14,836,202 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R3922:Nek10
|
UTSW |
14 |
14,861,585 (GRCm38) |
missense |
possibly damaging |
0.88 |
|
R4032:Nek10
|
UTSW |
14 |
14,853,877 (GRCm38) |
splice site |
probably null |
|
|
R4700:Nek10
|
UTSW |
14 |
14,842,841 (GRCm38) |
missense |
possibly damaging |
0.69 |
|
R4742:Nek10
|
UTSW |
14 |
14,861,624 (GRCm38) |
missense |
probably null |
0.03 |
|
R4785:Nek10
|
UTSW |
14 |
14,855,714 (GRCm38) |
missense |
probably benign |
|
|
R4890:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4891:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4920:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4924:Nek10
|
UTSW |
14 |
14,846,594 (GRCm38) |
splice site |
probably null |
|
|
R4928:Nek10
|
UTSW |
14 |
14,930,577 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4948:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4952:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4953:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R5092:Nek10
|
UTSW |
14 |
14,820,851 (GRCm38) |
missense |
possibly damaging |
0.81 |
|
R5097:Nek10
|
UTSW |
14 |
14,857,851 (GRCm38) |
missense |
probably benign |
0.00 |
|
R5593:Nek10
|
UTSW |
14 |
14,980,544 (GRCm38) |
nonsense |
probably null |
|
|
R5696:Nek10
|
UTSW |
14 |
14,860,736 (GRCm38) |
splice site |
probably null |
|
|
R5813:Nek10
|
UTSW |
14 |
14,986,704 (GRCm38) |
missense |
probably benign |
0.01 |
|
R5829:Nek10
|
UTSW |
14 |
14,865,404 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R5872:Nek10
|
UTSW |
14 |
14,850,896 (GRCm38) |
missense |
probably benign |
0.06 |
|
R5939:Nek10
|
UTSW |
14 |
14,931,290 (GRCm38) |
missense |
possibly damaging |
0.58 |
|
R6025:Nek10
|
UTSW |
14 |
14,865,633 (GRCm38) |
missense |
probably benign |
0.41 |
|
R6235:Nek10
|
UTSW |
14 |
14,821,113 (GRCm38) |
nonsense |
probably null |
|
|
R6539:Nek10
|
UTSW |
14 |
14,860,789 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R6542:Nek10
|
UTSW |
14 |
14,999,108 (GRCm38) |
missense |
probably benign |
0.44 |
|
R6561:Nek10
|
UTSW |
14 |
14,828,448 (GRCm38) |
missense |
possibly damaging |
0.48 |
|
R6659:Nek10
|
UTSW |
14 |
14,861,684 (GRCm38) |
missense |
probably benign |
0.29 |
|
R7039:Nek10
|
UTSW |
14 |
14,986,700 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R7039:Nek10
|
UTSW |
14 |
14,826,946 (GRCm38) |
missense |
possibly damaging |
0.63 |
|
R7102:Nek10
|
UTSW |
14 |
14,828,517 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R7185:Nek10
|
UTSW |
14 |
14,846,621 (GRCm38) |
missense |
probably benign |
0.03 |
|
R7198:Nek10
|
UTSW |
14 |
14,850,947 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R7251:Nek10
|
UTSW |
14 |
14,853,965 (GRCm38) |
missense |
probably benign |
|
|
R7345:Nek10
|
UTSW |
14 |
14,955,503 (GRCm38) |
missense |
probably benign |
|
|
R7590:Nek10
|
UTSW |
14 |
15,006,693 (GRCm38) |
makesense |
probably null |
|
|
R7593:Nek10
|
UTSW |
14 |
14,826,955 (GRCm38) |
missense |
probably benign |
0.04 |
|
R7616:Nek10
|
UTSW |
14 |
14,937,759 (GRCm38) |
missense |
probably benign |
0.27 |
|
R7635:Nek10
|
UTSW |
14 |
14,850,932 (GRCm38) |
missense |
probably benign |
0.01 |
|
R7817:Nek10
|
UTSW |
14 |
15,001,017 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7826:Nek10
|
UTSW |
14 |
14,860,846 (GRCm38) |
splice site |
probably null |
|
|
R7986:Nek10
|
UTSW |
14 |
15,001,020 (GRCm38) |
missense |
probably benign |
0.17 |
|
R8765:Nek10
|
UTSW |
14 |
14,999,104 (GRCm38) |
missense |
probably damaging |
0.97 |
|
R8856:Nek10
|
UTSW |
14 |
14,937,610 (GRCm38) |
missense |
probably damaging |
0.96 |
|
R8973:Nek10
|
UTSW |
14 |
14,931,321 (GRCm38) |
critical splice donor site |
probably null |
|
|
R9002:Nek10
|
UTSW |
14 |
14,980,590 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9088:Nek10
|
UTSW |
14 |
14,931,314 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9195:Nek10
|
UTSW |
14 |
14,821,139 (GRCm38) |
missense |
probably benign |
0.03 |
|
R9464:Nek10
|
UTSW |
14 |
14,937,766 (GRCm38) |
missense |
probably benign |
|
|
R9511:Nek10
|
UTSW |
14 |
14,828,511 (GRCm38) |
missense |
probably benign |
0.05 |
|
R9529:Nek10
|
UTSW |
14 |
14,850,833 (GRCm38) |
missense |
probably benign |
|
|
R9590:Nek10
|
UTSW |
14 |
14,853,888 (GRCm38) |
missense |
probably damaging |
1.00 |
|
Z1177:Nek10
|
UTSW |
14 |
15,001,157 (GRCm38) |
nonsense |
probably null |
|
|
Z1177:Nek10
|
UTSW |
14 |
14,853,948 (GRCm38) |
missense |
probably benign |
0.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- TCAGTTTGGAACCACAGTGG -3'
(R):5'- GGTCCTGACTAGTGACAGAAAC -3'
Sequencing Primer
(F):5'- ACAGTGGTGAAGATGGTTTCTG -3'
(R):5'- TCCTGACTAGTGACAGAAACATTAAC -3'
|
| Posted On |
2019-06-26 |
Incidental Mutation