Mutagenetix > Incidental Mutation

Incidental Mutation 'R7204:Cdc25b'

560422

Institutional Source

Beutler Lab

Gene Symbol

Cdc25b

Ensembl Gene

ENSMUSG00000027330

Gene Name

cell division cycle 25B

Synonyms

MMRRC Submission

045282-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7204 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

131028869-131040417 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 131033552 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 164 (I164F)

Ref Sequence

ENSEMBL: ENSMUSP00000028804 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000028801] [ENSMUST00000028804] [ENSMUST00000079857]

AlphaFold

P30306

Predicted Effect

probably benign
Transcript: ENSMUST00000028801

SMART Domains

Protein: ENSMUSP00000028801
Gene: ENSMUSG00000027329

Domain	Start	End	E-Value	Type
Pfam:CH_2	13	109	9.3e-36	PFAM
Pfam:CAMSAP_CH	14	96	7.9e-24	PFAM
coiled coil region	182	234	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000028804
AA Change: I164F

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000028804
Gene: ENSMUSG00000027330
AA Change: I164F

Domain	Start	End	E-Value	Type
low complexity region	86	105	N/A	INTRINSIC
Pfam:M-inducer_phosp	111	379	3.3e-103	PFAM
RHOD	417	531	4.29e-26	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000079857
AA Change: I164F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000078784
Gene: ENSMUSG00000027330
AA Change: I164F

Domain	Start	End	E-Value	Type
low complexity region	86	105	N/A	INTRINSIC
Pfam:M-inducer_phosp	111	354	2.4e-78	PFAM
RHOD	391	505	4.29e-26	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.6%

Validation Efficiency

99% (75/76)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008]
PHENOTYPE: The resumption of meiosis during oocyte maturation is blocked in homozygous mutant female mice, resulting in female infertility. Male mice do not show an overt reproductive phenotype. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700067K01Rik	T	C	8: 84,730,636 (GRCm39)	S227P	probably damaging	Het
Acot6	A	G	12: 84,153,301 (GRCm39)	H181R	probably benign	Het
Adam15	A	T	3: 89,254,244 (GRCm39)	H184Q	probably benign	Het
Agl	A	G	3: 116,587,469 (GRCm39)	F29L	probably benign	Het
Ak7	A	G	12: 105,708,502 (GRCm39)	Y319C	probably benign	Het
Ano8	C	A	8: 71,931,669 (GRCm39)	V813L	probably benign	Het
Apbb2	T	G	5: 66,608,946 (GRCm39)	K234Q	probably damaging	Het
Arfgef3	A	T	10: 18,522,210 (GRCm39)	D605E	probably damaging	Het
Bfsp2	C	A	9: 103,309,865 (GRCm39)	R340L	probably damaging	Het
Birc6	C	A	17: 74,947,103 (GRCm39)	P2956T	probably damaging	Het
C1rb	T	C	6: 124,554,386 (GRCm39)	I389T	probably benign	Het
Ccdc146	A	G	5: 21,513,624 (GRCm39)	F498S	probably benign	Het
Cdc14b	A	C	13: 64,358,012 (GRCm39)	V361G	possibly damaging	Het
Col18a1	A	G	10: 76,921,110 (GRCm39)	S297P	unknown	Het
Col3a1	A	T	1: 45,361,578 (GRCm39)	I117F	unknown	Het
Cox4i2	AG	A	2: 152,602,618 (GRCm39)		probably null	Het
Crybg3	C	A	16: 59,379,253 (GRCm39)	G667V	probably benign	Het
Ctc1	A	G	11: 68,920,567 (GRCm39)	D623G	probably damaging	Het
Dennd1a	A	T	2: 37,929,215 (GRCm39)	H152Q	probably damaging	Het
Dgke	G	A	11: 88,932,306 (GRCm39)	P495S	probably damaging	Het
Dll3	A	T	7: 27,998,330 (GRCm39)	C212S	possibly damaging	Het
Dll4	A	T	2: 119,159,054 (GRCm39)	S235C	probably damaging	Het
Dnah12	G	A	14: 26,500,869 (GRCm39)		probably null	Het
Dnah12	A	T	14: 26,503,442 (GRCm39)	T1599S	probably damaging	Het
Elavl1	G	A	8: 4,361,712 (GRCm39)	T20M	probably damaging	Het
Epha3	T	C	16: 63,472,695 (GRCm39)	T397A	probably benign	Het
Fanca	A	T	8: 124,013,216 (GRCm39)	I859N	probably damaging	Het
Fzd1	A	T	5: 4,805,980 (GRCm39)	V534D	probably damaging	Het
Glmp	A	G	3: 88,233,917 (GRCm39)	Y258C	probably damaging	Het
Gm9195	G	A	14: 72,711,626 (GRCm39)	P329S	probably damaging	Het
Gpt	A	G	15: 76,583,199 (GRCm39)	R379G	probably benign	Het
Gsdmc2	T	C	15: 63,696,903 (GRCm39)	T423A	probably damaging	Het
Hemgn	T	C	4: 46,397,054 (GRCm39)	K61E	possibly damaging	Het
Ikzf4	A	T	10: 128,479,759 (GRCm39)	S56T	possibly damaging	Het
Jak1	T	C	4: 101,032,332 (GRCm39)	T425A	probably benign	Het
Jsrp1	T	C	10: 80,646,319 (GRCm39)	T80A	probably benign	Het
Klra9	T	A	6: 130,165,643 (GRCm39)	D124V	possibly damaging	Het
Lama5	A	T	2: 179,843,970 (GRCm39)	V397D	probably damaging	Het
Lrp2	A	G	2: 69,302,877 (GRCm39)	S2951P	probably benign	Het
Map7d1	A	G	4: 126,149,808 (GRCm39)		probably null	Het
Mcm6	A	T	1: 128,265,864 (GRCm39)	C636S	probably damaging	Het
Mia	T	C	7: 26,880,358 (GRCm39)	E39G	possibly damaging	Het
Mpeg1	T	A	19: 12,440,258 (GRCm39)	I572N	probably damaging	Het
Muc21	AGCTGGATGCAGTGGTGGTCAGGGTGGGTGTAGAGCCTGAGCCAGTGCTGGATACAGTGGTGGTC	AGCTGGATACAGTGGTGGTC	17: 35,932,105 (GRCm39)		probably benign	Het
Muc6	AGGCGCAGAAACCCTGGC	AGGC	7: 141,214,363 (GRCm39)		probably null	Het
Mup5	A	G	4: 61,751,992 (GRCm39)	Y86H	probably damaging	Het
Nckap5	A	T	1: 125,954,104 (GRCm39)	V816D	probably benign	Het
Nfib	T	C	4: 82,215,052 (GRCm39)		probably null	Het
Nlrc5	T	A	8: 95,218,153 (GRCm39)	V1056D	possibly damaging	Het
Nynrin	G	C	14: 56,110,190 (GRCm39)	E1766Q	probably damaging	Het
Or7d9	A	T	9: 20,197,100 (GRCm39)	Y43F	probably benign	Het
Oxct1	T	A	15: 4,123,524 (GRCm39)	L328Q	probably damaging	Het
Pax5	T	A	4: 44,679,485 (GRCm39)	I187F	possibly damaging	Het
Pcdhb4	A	T	18: 37,442,292 (GRCm39)	D534V	probably damaging	Het
Pebp4	C	T	14: 70,085,046 (GRCm39)	P35S	probably benign	Het
Pkhd1l1	T	A	15: 44,386,949 (GRCm39)	V1274E	possibly damaging	Het
Plcz1	A	T	6: 139,956,150 (GRCm39)	V373D	probably benign	Het
Plxnb1	C	T	9: 108,929,243 (GRCm39)	T33I	probably damaging	Het
Prag1	T	C	8: 36,613,915 (GRCm39)	C1156R	probably benign	Het
Ptprc	T	A	1: 138,045,600 (GRCm39)	I87F	probably benign	Het
Rbp4	G	A	19: 38,112,551 (GRCm39)	T138I	possibly damaging	Het
Semp2l2b	A	G	10: 21,943,785 (GRCm39)	L65P	probably damaging	Het
Slc12a1	A	T	2: 125,042,542 (GRCm39)	N733Y	possibly damaging	Het
Slc27a3	A	G	3: 90,297,033 (GRCm39)	V22A	probably benign	Het
Tcaf1	T	C	6: 42,651,973 (GRCm39)		probably null	Het
Tnc	T	C	4: 63,889,392 (GRCm39)		probably null	Het
Tspan31	A	T	10: 126,903,987 (GRCm39)	*211R	probably null	Het
Ttc17	A	T	2: 94,192,773 (GRCm39)	V86D	possibly damaging	Het
Ubr1	T	A	2: 120,734,558 (GRCm39)	N1114I	possibly damaging	Het
Ulk2	C	T	11: 61,674,457 (GRCm39)	G850R	probably benign	Het
Vmn1r13	T	A	6: 57,187,141 (GRCm39)	I100N	probably benign	Het
Zan	T	C	5: 137,426,240 (GRCm39)	D2512G	unknown	Het
Zc3h14	A	T	12: 98,737,615 (GRCm39)	N34I	probably damaging	Het
Zcwpw1	T	G	5: 137,810,346 (GRCm39)	L374R	probably damaging	Het
Zfp747l1	A	T	7: 126,983,518 (GRCm39)	I528K	possibly damaging	Het

Other mutations in Cdc25b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03230:Cdc25b	APN	2	131,030,060 (GRCm39)	missense	probably benign	0.00
R0471:Cdc25b	UTSW	2	131,039,204 (GRCm39)	missense	probably damaging	0.99
R0639:Cdc25b	UTSW	2	131,039,182 (GRCm39)	missense	probably benign	0.00
R0645:Cdc25b	UTSW	2	131,033,533 (GRCm39)	missense	probably benign	0.06
R0673:Cdc25b	UTSW	2	131,039,182 (GRCm39)	missense	probably benign	0.00
R1574:Cdc25b	UTSW	2	131,033,057 (GRCm39)	splice site	probably benign
R4094:Cdc25b	UTSW	2	131,031,037 (GRCm39)	missense	probably benign
R4433:Cdc25b	UTSW	2	131,033,618 (GRCm39)	missense	probably benign	0.02
R4722:Cdc25b	UTSW	2	131,035,271 (GRCm39)	missense	probably damaging	1.00
R4817:Cdc25b	UTSW	2	131,035,223 (GRCm39)	missense	probably damaging	1.00
R4957:Cdc25b	UTSW	2	131,035,525 (GRCm39)	missense	possibly damaging	0.80
R5345:Cdc25b	UTSW	2	131,034,516 (GRCm39)	missense	probably benign	0.18
R5407:Cdc25b	UTSW	2	131,035,567 (GRCm39)	missense	probably damaging	1.00
R5562:Cdc25b	UTSW	2	131,036,678 (GRCm39)	missense	probably damaging	1.00
R5594:Cdc25b	UTSW	2	131,033,538 (GRCm39)	missense	probably damaging	1.00
R5792:Cdc25b	UTSW	2	131,033,679 (GRCm39)	missense	probably damaging	1.00
R5831:Cdc25b	UTSW	2	131,029,301 (GRCm39)	critical splice donor site	probably null
R7292:Cdc25b	UTSW	2	131,033,093 (GRCm39)	missense	probably damaging	1.00
R7399:Cdc25b	UTSW	2	131,036,574 (GRCm39)	missense	probably damaging	1.00
R7501:Cdc25b	UTSW	2	131,036,080 (GRCm39)	missense	probably damaging	1.00
R7772:Cdc25b	UTSW	2	131,031,029 (GRCm39)	missense	probably damaging	1.00
R8186:Cdc25b	UTSW	2	131,031,050 (GRCm39)	missense	probably benign	0.05
R8783:Cdc25b	UTSW	2	131,033,772 (GRCm39)	missense	probably benign	0.05
R8985:Cdc25b	UTSW	2	131,035,180 (GRCm39)	missense	probably damaging	1.00
R9141:Cdc25b	UTSW	2	131,033,857 (GRCm39)	critical splice donor site	probably null
R9153:Cdc25b	UTSW	2	131,034,564 (GRCm39)	missense	possibly damaging	0.84

Predicted Primers

PCR Primer
(F):5'- CTTAGGCATCCTGGTTGGCATG -3'
(R):5'- ATCCTGTGGGCAAGATTGGAG -3'

Sequencing Primer
(F):5'- CATCCTGGTTGGCATGGACATC -3'
(R):5'- GAGGAACTATCAGGATAAACCGAGTG -3'

Posted On

2019-06-26