Mutagenetix > Incidental Mutation

Incidental Mutation 'R7204:Zan'

560437

Institutional Source

Beutler Lab

Gene Symbol

Zan

Ensembl Gene

ENSMUSG00000079173

Gene Name

zonadhesin

Synonyms

MMRRC Submission

045282-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R7204 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

137378637-137477064 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 137427978 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 2512 (D2512G)

Ref Sequence

ENSEMBL: ENSMUSP00000114068 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000117564] [ENSMUST00000164178]

AlphaFold

no structure available at present

Predicted Effect

unknown
Transcript: ENSMUST00000117564
AA Change: D2512G

SMART Domains

Protein: ENSMUSP00000114068
Gene: ENSMUSG00000079173
AA Change: D2512G

Domain	Start	End	E-Value	Type
signal peptide	1	17	N/A	INTRINSIC
MAM	42	210	3.55e-20	SMART
MAM	214	374	3.97e-9	SMART
MAM	375	542	5.7e-42	SMART
low complexity region	549	563	N/A	INTRINSIC
low complexity region	578	607	N/A	INTRINSIC
low complexity region	637	648	N/A	INTRINSIC
low complexity region	672	689	N/A	INTRINSIC
low complexity region	702	779	N/A	INTRINSIC
low complexity region	782	865	N/A	INTRINSIC
low complexity region	890	913	N/A	INTRINSIC
low complexity region	941	1038	N/A	INTRINSIC
low complexity region	1043	1084	N/A	INTRINSIC
low complexity region	1096	1131	N/A	INTRINSIC
low complexity region	1136	1147	N/A	INTRINSIC
low complexity region	1150	1167	N/A	INTRINSIC
low complexity region	1178	1192	N/A	INTRINSIC
EGF_like	1236	1259	7.09e1	SMART
VWC	1266	1356	5e-3	SMART
VWD	1316	1477	3.73e-36	SMART
C8	1521	1596	6.91e-23	SMART
EGF_like	1607	1647	6.41e1	SMART
VWC	1654	1745	1.08e-2	SMART
VWD	1703	1869	2.71e-47	SMART
C8	1908	1982	3.45e-32	SMART
Pfam:TIL	1985	2039	4.5e-13	PFAM
VWC	2041	2095	4.84e-1	SMART
FOLN	2074	2096	9.79e1	SMART
VWD	2088	2260	5.49e-25	SMART
C8	2307	2381	6.73e-3	SMART
Pfam:TIL	2384	2442	3e-12	PFAM
VWC	2444	2504	1.13e-1	SMART
FOLN	2475	2498	3.73e0	SMART
EGF_like	2512	2557	6.54e1	SMART
VWC	2564	2637	3.68e-2	SMART
FOLN	2595	2618	4.04e0	SMART
VWC	2684	2744	3.08e-1	SMART
FOLN	2715	2738	7.78e0	SMART
VWC	2804	2864	1.7e0	SMART
FOLN	2835	2858	2.58e1	SMART
VWC	2924	2984	4.74e-1	SMART
VWC	3044	3104	2.44e-1	SMART
VWC	3164	3237	7.57e-2	SMART
FOLN	3195	3218	2.25e1	SMART
VWC	3284	3344	4.22e-1	SMART
FOLN	3315	3338	2.1e0	SMART
VWC	3401	3461	7.67e-2	SMART
FOLN	3432	3455	4.39e0	SMART
VWC	3521	3581	8.45e-2	SMART
FOLN	3552	3575	1.27e1	SMART
VWC	3641	3714	3.51e-1	SMART
FOLN	3672	3695	2.16e0	SMART
VWC	3761	3821	9.7e-2	SMART
FOLN	3792	3815	1.27e1	SMART
VWC	3881	3954	1.83e-1	SMART
FOLN	3912	3933	1.17e1	SMART
VWC	3997	4050	3.61e-1	SMART
FOLN	4028	4051	1.84e0	SMART
EGF_like	4081	4126	5.79e1	SMART
VWC	4133	4210	4.03e-1	SMART
FOLN	4164	4187	7.99e0	SMART
VWC	4253	4308	3.21e-1	SMART
FOLN	4284	4302	8.54e1	SMART
VWC	4368	4428	2.74e-2	SMART
FOLN	4399	4422	7.46e1	SMART
VWC	4488	4548	6.37e-1	SMART
FOLN	4519	4542	1.04e0	SMART
VWC	4608	4681	4.47e-1	SMART
FOLN	4639	4662	2.22e0	SMART
VWC	4728	4781	1.12e0	SMART
FOLN	4759	4782	3.29e1	SMART
EGF	4800	4841	2.43e1	SMART
VWC	4848	4901	7.59e-1	SMART
FOLN	4879	4902	5.31e0	SMART
VWD	4899	5061	4.49e-30	SMART
low complexity region	5086	5102	N/A	INTRINSIC
C8	5113	5191	8.25e-21	SMART
Pfam:TIL	5194	5247	1.9e-12	PFAM
VWC	5249	5307	1.22e0	SMART
EGF	5306	5339	3.15e-3	SMART
transmembrane domain	5356	5378	N/A	INTRINSIC
low complexity region	5381	5399	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000164178
AA Change: D2512G

SMART Domains

Protein: ENSMUSP00000132895
Gene: ENSMUSG00000079173
AA Change: D2512G

Domain	Start	End	E-Value	Type
signal peptide	1	17	N/A	INTRINSIC
MAM	42	210	3.55e-20	SMART
MAM	214	374	3.97e-9	SMART
MAM	375	542	5.7e-42	SMART
low complexity region	549	563	N/A	INTRINSIC
low complexity region	578	607	N/A	INTRINSIC
low complexity region	637	648	N/A	INTRINSIC
low complexity region	672	689	N/A	INTRINSIC
low complexity region	702	779	N/A	INTRINSIC
low complexity region	782	865	N/A	INTRINSIC
low complexity region	890	913	N/A	INTRINSIC
low complexity region	941	1038	N/A	INTRINSIC
low complexity region	1043	1084	N/A	INTRINSIC
low complexity region	1096	1131	N/A	INTRINSIC
low complexity region	1136	1147	N/A	INTRINSIC
low complexity region	1150	1167	N/A	INTRINSIC
low complexity region	1178	1192	N/A	INTRINSIC
EGF_like	1236	1259	7.09e1	SMART
VWC	1266	1356	5e-3	SMART
VWD	1316	1477	3.73e-36	SMART
C8	1521	1596	6.91e-23	SMART
EGF_like	1607	1647	6.41e1	SMART
VWC	1654	1745	1.08e-2	SMART
VWD	1703	1869	2.71e-47	SMART
C8	1908	1982	3.45e-32	SMART
Pfam:TIL	1985	2039	2.3e-13	PFAM
VWC	2041	2095	4.84e-1	SMART
FOLN	2074	2096	9.79e1	SMART
VWD	2088	2260	5.49e-25	SMART
C8	2307	2381	6.73e-3	SMART
Pfam:TIL	2384	2442	6e-12	PFAM
VWC	2444	2504	1.13e-1	SMART
FOLN	2475	2498	3.73e0	SMART
EGF_like	2512	2557	6.54e1	SMART
VWC	2564	2637	3.68e-2	SMART
FOLN	2595	2618	4.04e0	SMART
VWC	2684	2744	3.08e-1	SMART
FOLN	2715	2738	7.78e0	SMART
VWC	2804	2864	1.7e0	SMART
FOLN	2835	2858	2.58e1	SMART
VWC	2924	2984	4.74e-1	SMART
VWC	3044	3104	2.44e-1	SMART
VWC	3164	3237	7.57e-2	SMART
FOLN	3195	3218	2.25e1	SMART
VWC	3284	3344	4.22e-1	SMART
FOLN	3315	3338	2.1e0	SMART
VWC	3401	3461	7.67e-2	SMART
FOLN	3432	3455	4.39e0	SMART
VWC	3521	3581	8.45e-2	SMART
FOLN	3552	3575	1.27e1	SMART
VWC	3641	3714	3.51e-1	SMART
FOLN	3672	3695	2.16e0	SMART
VWC	3761	3821	9.7e-2	SMART
FOLN	3792	3815	1.27e1	SMART
VWC	3881	3954	1.83e-1	SMART
FOLN	3912	3933	1.17e1	SMART
VWC	3997	4050	3.61e-1	SMART
FOLN	4028	4051	1.84e0	SMART
EGF_like	4081	4126	5.79e1	SMART
VWC	4133	4210	4.03e-1	SMART
FOLN	4164	4187	7.99e0	SMART
VWC	4253	4308	3.21e-1	SMART
FOLN	4284	4302	8.54e1	SMART
VWC	4368	4428	2.74e-2	SMART
FOLN	4399	4422	7.46e1	SMART
VWC	4488	4548	6.37e-1	SMART
FOLN	4519	4542	1.04e0	SMART
VWC	4608	4681	4.47e-1	SMART
FOLN	4639	4662	2.22e0	SMART
VWC	4728	4781	1.12e0	SMART
FOLN	4759	4782	3.29e1	SMART
EGF	4800	4841	2.43e1	SMART
VWC	4848	4901	7.59e-1	SMART
FOLN	4879	4902	5.31e0	SMART
VWD	4899	5061	4.49e-30	SMART
low complexity region	5086	5102	N/A	INTRINSIC
C8	5113	5191	8.25e-21	SMART
Pfam:TIL	5194	5247	7.2e-13	PFAM
VWC	5249	5307	1.22e0	SMART
EGF	5306	5339	3.15e-3	SMART
transmembrane domain	5356	5378	N/A	INTRINSIC
low complexity region	5381	5399	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.6%

Validation Efficiency

99% (75/76)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that functions in the species specificity of sperm adhesion to the egg zona pellucida. The encoded protein is located in the acrosome and may be involved in signaling or gamete recognition. An allelic polymorphism in this gene results in both functional and frameshifted alleles; the reference genome represents the functional allele. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2015]
PHENOTYPE: Sperm from mice homozygous for a knock-out allele exhibit decreased species-specific zona pellucida adhesion without alteration in fertility. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700067K01Rik	T	C	8: 84,004,007 (GRCm38)	S227P	probably damaging	Het
Acot6	A	G	12: 84,106,527 (GRCm38)	H181R	probably benign	Het
Adam15	A	T	3: 89,346,937 (GRCm38)	H184Q	probably benign	Het
Agl	A	G	3: 116,793,820 (GRCm38)	F29L	probably benign	Het
Ak7	A	G	12: 105,742,243 (GRCm38)	Y319C	probably benign	Het
Ano8	C	A	8: 71,479,025 (GRCm38)	V813L	probably benign	Het
Apbb2	T	G	5: 66,451,603 (GRCm38)	K234Q	probably damaging	Het
Arfgef3	A	T	10: 18,646,462 (GRCm38)	D605E	probably damaging	Het
Bfsp2	C	A	9: 103,432,666 (GRCm38)	R340L	probably damaging	Het
Birc6	C	A	17: 74,640,108 (GRCm38)	P2956T	probably damaging	Het
C1rb	T	C	6: 124,577,427 (GRCm38)	I389T	probably benign	Het
Ccdc146	A	G	5: 21,308,626 (GRCm38)	F498S	probably benign	Het
Cdc14b	A	C	13: 64,210,198 (GRCm38)	V361G	possibly damaging	Het
Cdc25b	A	T	2: 131,191,632 (GRCm38)	I164F	probably damaging	Het
Col18a1	A	G	10: 77,085,276 (GRCm38)	S297P	unknown	Het
Col3a1	A	T	1: 45,322,418 (GRCm38)	I117F	unknown	Het
Cox4i2	AG	A	2: 152,760,698 (GRCm38)		probably null	Het
Crybg3	C	A	16: 59,558,890 (GRCm38)	G667V	probably benign	Het
Ctc1	A	G	11: 69,029,741 (GRCm38)	D623G	probably damaging	Het
Dennd1a	A	T	2: 38,039,203 (GRCm38)	H152Q	probably damaging	Het
Dgke	G	A	11: 89,041,480 (GRCm38)	P495S	probably damaging	Het
Dll3	A	T	7: 28,298,905 (GRCm38)	C212S	possibly damaging	Het
Dll4	A	T	2: 119,328,573 (GRCm38)	S235C	probably damaging	Het
Dnah12	A	T	14: 26,781,485 (GRCm38)	T1599S	probably damaging	Het
Dnah12	G	A	14: 26,778,912 (GRCm38)		probably null	Het
Elavl1	G	A	8: 4,311,712 (GRCm38)	T20M	probably damaging	Het
Epha3	T	C	16: 63,652,332 (GRCm38)	T397A	probably benign	Het
Fanca	A	T	8: 123,286,477 (GRCm38)	I859N	probably damaging	Het
Fzd1	A	T	5: 4,755,980 (GRCm38)	V534D	probably damaging	Het
Glmp	A	G	3: 88,326,610 (GRCm38)	Y258C	probably damaging	Het
Gm9195	G	A	14: 72,474,186 (GRCm38)	P329S	probably damaging	Het
Gpt	A	G	15: 76,698,999 (GRCm38)	R379G	probably benign	Het
Gsdmc2	T	C	15: 63,825,054 (GRCm38)	T423A	probably damaging	Het
Hemgn	T	C	4: 46,397,054 (GRCm38)	K61E	possibly damaging	Het
Ikzf4	A	T	10: 128,643,890 (GRCm38)	S56T	possibly damaging	Het
Jak1	T	C	4: 101,175,135 (GRCm38)	T425A	probably benign	Het
Jsrp1	T	C	10: 80,810,485 (GRCm38)	T80A	probably benign	Het
Klra9	T	A	6: 130,188,680 (GRCm38)	D124V	possibly damaging	Het
Lama5	A	T	2: 180,202,177 (GRCm38)	V397D	probably damaging	Het
Lrp2	A	G	2: 69,472,533 (GRCm38)	S2951P	probably benign	Het
Map7d1	A	G	4: 126,256,015 (GRCm38)		probably null	Het
Mcm6	A	T	1: 128,338,127 (GRCm38)	C636S	probably damaging	Het
Mia	T	C	7: 27,180,933 (GRCm38)	E39G	possibly damaging	Het
Mpeg1	T	A	19: 12,462,894 (GRCm38)	I572N	probably damaging	Het
Muc21	AGCTGGATGCAGTGGTGGTCAGGGTGGGTGTAGAGCCTGAGCCAGTGCTGGATACAGTGGTGGTC	AGCTGGATACAGTGGTGGTC	17: 35,621,213 (GRCm38)		probably benign	Het
Muc6	AGGCGCAGAAACCCTGGC	AGGC	7: 141,634,450 (GRCm38)		probably null	Het
Mup5	A	G	4: 61,833,755 (GRCm38)	Y86H	probably damaging	Het
Nckap5	A	T	1: 126,026,367 (GRCm38)	V816D	probably benign	Het
Nfib	T	C	4: 82,296,815 (GRCm38)		probably null	Het
Nlrc5	T	A	8: 94,491,525 (GRCm38)	V1056D	possibly damaging	Het
Nynrin	G	C	14: 55,872,733 (GRCm38)	E1766Q	probably damaging	Het
Or7d9	A	T	9: 20,285,804 (GRCm38)	Y43F	probably benign	Het
Oxct1	T	A	15: 4,094,042 (GRCm38)	L328Q	probably damaging	Het
Pax5	T	A	4: 44,679,485 (GRCm38)	I187F	possibly damaging	Het
Pcdhb4	A	T	18: 37,309,239 (GRCm38)	D534V	probably damaging	Het
Pebp4	C	T	14: 69,847,597 (GRCm38)	P35S	probably benign	Het
Pkhd1l1	T	A	15: 44,523,553 (GRCm38)	V1274E	possibly damaging	Het
Plcz1	A	T	6: 140,010,424 (GRCm38)	V373D	probably benign	Het
Plxnb1	C	T	9: 109,100,175 (GRCm38)	T33I	probably damaging	Het
Prag1	T	C	8: 36,146,761 (GRCm38)	C1156R	probably benign	Het
Ptprc	T	A	1: 138,117,862 (GRCm38)	I87F	probably benign	Het
Rbp4	G	A	19: 38,124,103 (GRCm38)	T138I	possibly damaging	Het
Semp2l2b	A	G	10: 22,067,886 (GRCm38)	L65P	probably damaging	Het
Slc12a1	A	T	2: 125,200,622 (GRCm38)	N733Y	possibly damaging	Het
Slc27a3	A	G	3: 90,389,726 (GRCm38)	V22A	probably benign	Het
Tcaf1	T	C	6: 42,675,039 (GRCm38)		probably null	Het
Tnc	T	C	4: 63,971,155 (GRCm38)		probably null	Het
Tspan31	A	T	10: 127,068,118 (GRCm38)	*211R	probably null	Het
Ttc17	A	T	2: 94,362,428 (GRCm38)	V86D	possibly damaging	Het
Ubr1	T	A	2: 120,904,077 (GRCm38)	N1114I	possibly damaging	Het
Ulk2	C	T	11: 61,783,631 (GRCm38)	G850R	probably benign	Het
Vmn1r13	T	A	6: 57,210,156 (GRCm38)	I100N	probably benign	Het
Zc3h14	A	T	12: 98,771,356 (GRCm38)	N34I	probably damaging	Het
Zcwpw1	T	G	5: 137,812,084 (GRCm38)	L374R	probably damaging	Het
Zfp747l1	A	T	7: 127,384,346 (GRCm38)	I528K	possibly damaging	Het

Other mutations in Zan

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00087:Zan	APN	5	137,387,820 (GRCm38)	critical splice donor site	probably null
IGL00158:Zan	APN	5	137,454,257 (GRCm38)	missense	unknown
IGL00473:Zan	APN	5	137,464,250 (GRCm38)	missense	possibly damaging	0.68
IGL00536:Zan	APN	5	137,446,682 (GRCm38)	missense	unknown
IGL00567:Zan	APN	5	137,416,277 (GRCm38)	unclassified	probably benign
IGL00820:Zan	APN	5	137,386,364 (GRCm38)	missense	unknown
IGL00850:Zan	APN	5	137,464,113 (GRCm38)	missense	unknown
IGL00906:Zan	APN	5	137,389,360 (GRCm38)	missense	unknown
IGL00920:Zan	APN	5	137,464,524 (GRCm38)	missense	unknown
IGL00964:Zan	APN	5	137,405,941 (GRCm38)	unclassified	probably benign
IGL01356:Zan	APN	5	137,436,432 (GRCm38)	missense	unknown
IGL01361:Zan	APN	5	137,414,342 (GRCm38)	unclassified	probably benign
IGL01362:Zan	APN	5	137,452,450 (GRCm38)	missense	unknown
IGL01411:Zan	APN	5	137,388,893 (GRCm38)	missense	unknown
IGL01412:Zan	APN	5	137,393,032 (GRCm38)	missense	unknown
IGL01531:Zan	APN	5	137,424,612 (GRCm38)	missense	unknown
IGL01561:Zan	APN	5	137,463,866 (GRCm38)	missense	unknown
IGL01564:Zan	APN	5	137,446,733 (GRCm38)	missense	unknown
IGL01568:Zan	APN	5	137,464,844 (GRCm38)	missense	unknown
IGL01719:Zan	APN	5	137,395,654 (GRCm38)	missense	unknown
IGL01732:Zan	APN	5	137,393,011 (GRCm38)	missense	unknown
IGL01761:Zan	APN	5	137,425,597 (GRCm38)	missense	unknown
IGL01771:Zan	APN	5	137,393,068 (GRCm38)	missense	unknown
IGL01810:Zan	APN	5	137,463,626 (GRCm38)	missense	unknown
IGL01845:Zan	APN	5	137,380,854 (GRCm38)	unclassified	probably benign
IGL01885:Zan	APN	5	137,464,124 (GRCm38)	missense	unknown
IGL01992:Zan	APN	5	137,424,106 (GRCm38)	missense	unknown
IGL02026:Zan	APN	5	137,405,464 (GRCm38)	unclassified	probably benign
IGL02065:Zan	APN	5	137,386,960 (GRCm38)	nonsense	probably null
IGL02133:Zan	APN	5	137,411,498 (GRCm38)	missense	possibly damaging	0.84
IGL02274:Zan	APN	5	137,421,167 (GRCm38)	missense	unknown
IGL02449:Zan	APN	5	137,389,327 (GRCm38)	missense	unknown
IGL02456:Zan	APN	5	137,446,844 (GRCm38)	missense	unknown
IGL02493:Zan	APN	5	137,435,706 (GRCm38)	missense	unknown
IGL02496:Zan	APN	5	137,464,794 (GRCm38)	nonsense	probably null
IGL02528:Zan	APN	5	137,465,141 (GRCm38)	missense	possibly damaging	0.83
IGL02550:Zan	APN	5	137,387,039 (GRCm38)	missense	unknown
IGL02598:Zan	APN	5	137,446,211 (GRCm38)	missense	unknown
IGL02697:Zan	APN	5	137,400,548 (GRCm38)	missense	unknown
IGL02933:Zan	APN	5	137,428,414 (GRCm38)	missense	unknown
IGL02963:Zan	APN	5	137,456,250 (GRCm38)	missense	unknown
IGL02972:Zan	APN	5	137,463,686 (GRCm38)	missense	unknown
IGL03068:Zan	APN	5	137,476,415 (GRCm38)	missense	probably damaging	1.00
IGL03104:Zan	APN	5	137,463,500 (GRCm38)	missense	unknown
IGL03110:Zan	APN	5	137,420,016 (GRCm38)	missense	unknown
IGL03156:Zan	APN	5	137,463,939 (GRCm38)	missense	unknown
IGL03302:Zan	APN	5	137,468,390 (GRCm38)	missense	possibly damaging	0.93
IGL03307:Zan	APN	5	137,474,025 (GRCm38)	missense	probably damaging	0.99
IGL03340:Zan	APN	5	137,427,874 (GRCm38)	missense	unknown
IGL03379:Zan	APN	5	137,464,215 (GRCm38)	missense	unknown
IGL03405:Zan	APN	5	137,424,597 (GRCm38)	missense	unknown
Befallen	UTSW	5	137,412,676 (GRCm38)	unclassified	probably benign
befell	UTSW	5	137,446,037 (GRCm38)	splice site	probably null
R3853_zan_008	UTSW	5	137,474,064 (GRCm38)	missense	probably damaging	1.00
BB010:Zan	UTSW	5	137,463,579 (GRCm38)	missense	unknown
BB020:Zan	UTSW	5	137,463,579 (GRCm38)	missense	unknown
G1patch:Zan	UTSW	5	137,438,520 (GRCm38)	missense	unknown
PIT4283001:Zan	UTSW	5	137,400,093 (GRCm38)	missense	unknown
PIT4431001:Zan	UTSW	5	137,392,064 (GRCm38)	missense	unknown
PIT4498001:Zan	UTSW	5	137,417,036 (GRCm38)	critical splice donor site	probably null
R0027:Zan	UTSW	5	137,406,519 (GRCm38)	unclassified	probably benign
R0047:Zan	UTSW	5	137,403,656 (GRCm38)	missense	unknown
R0149:Zan	UTSW	5	137,396,766 (GRCm38)	missense	unknown
R0240:Zan	UTSW	5	137,398,362 (GRCm38)	missense	unknown
R0240:Zan	UTSW	5	137,398,362 (GRCm38)	missense	unknown
R0241:Zan	UTSW	5	137,421,822 (GRCm38)	missense	unknown
R0241:Zan	UTSW	5	137,421,822 (GRCm38)	missense	unknown
R0361:Zan	UTSW	5	137,396,766 (GRCm38)	missense	unknown
R0432:Zan	UTSW	5	137,382,316 (GRCm38)	unclassified	probably benign
R0436:Zan	UTSW	5	137,464,902 (GRCm38)	missense	unknown
R0446:Zan	UTSW	5	137,391,658 (GRCm38)	missense	unknown
R0457:Zan	UTSW	5	137,407,706 (GRCm38)	unclassified	probably benign
R0478:Zan	UTSW	5	137,400,526 (GRCm38)	splice site	probably benign
R0487:Zan	UTSW	5	137,413,358 (GRCm38)	critical splice donor site	probably null
R0497:Zan	UTSW	5	137,412,676 (GRCm38)	unclassified	probably benign
R0504:Zan	UTSW	5	137,470,318 (GRCm38)	missense	probably damaging	1.00
R0545:Zan	UTSW	5	137,396,177 (GRCm38)	missense	unknown
R0556:Zan	UTSW	5	137,454,220 (GRCm38)	missense	unknown
R0615:Zan	UTSW	5	137,468,431 (GRCm38)	missense	probably damaging	1.00
R0737:Zan	UTSW	5	137,389,249 (GRCm38)	missense	unknown
R0835:Zan	UTSW	5	137,408,397 (GRCm38)	unclassified	probably benign
R0863:Zan	UTSW	5	137,458,639 (GRCm38)	missense	unknown
R0971:Zan	UTSW	5	137,434,063 (GRCm38)	missense	unknown
R1327:Zan	UTSW	5	137,465,911 (GRCm38)	splice site	probably benign
R1338:Zan	UTSW	5	137,393,651 (GRCm38)	nonsense	probably null
R1413:Zan	UTSW	5	137,427,939 (GRCm38)	missense	unknown
R1446:Zan	UTSW	5	137,389,360 (GRCm38)	missense	unknown
R1464:Zan	UTSW	5	137,419,929 (GRCm38)	missense	unknown
R1464:Zan	UTSW	5	137,419,929 (GRCm38)	missense	unknown
R1561:Zan	UTSW	5	137,380,838 (GRCm38)	nonsense	probably null
R1569:Zan	UTSW	5	137,429,130 (GRCm38)	missense	unknown
R1575:Zan	UTSW	5	137,461,952 (GRCm38)	missense	unknown
R1618:Zan	UTSW	5	137,383,830 (GRCm38)	missense	unknown
R1634:Zan	UTSW	5	137,412,790 (GRCm38)	unclassified	probably benign
R1650:Zan	UTSW	5	137,394,601 (GRCm38)	splice site	probably benign
R1680:Zan	UTSW	5	137,403,050 (GRCm38)	missense	unknown
R1698:Zan	UTSW	5	137,409,669 (GRCm38)	utr 3 prime	probably benign
R1704:Zan	UTSW	5	137,434,002 (GRCm38)	nonsense	probably null
R1728:Zan	UTSW	5	137,415,018 (GRCm38)	unclassified	probably benign
R1729:Zan	UTSW	5	137,415,018 (GRCm38)	unclassified	probably benign
R1769:Zan	UTSW	5	137,464,518 (GRCm38)	missense	unknown
R1774:Zan	UTSW	5	137,419,989 (GRCm38)	missense	unknown
R1800:Zan	UTSW	5	137,386,451 (GRCm38)	missense	unknown
R1858:Zan	UTSW	5	137,405,877 (GRCm38)	unclassified	probably benign
R1888:Zan	UTSW	5	137,389,328 (GRCm38)	missense	unknown
R1888:Zan	UTSW	5	137,389,328 (GRCm38)	missense	unknown
R1925:Zan	UTSW	5	137,425,642 (GRCm38)	missense	unknown
R1938:Zan	UTSW	5	137,388,939 (GRCm38)	missense	unknown
R1955:Zan	UTSW	5	137,389,283 (GRCm38)	missense	unknown
R1989:Zan	UTSW	5	137,420,006 (GRCm38)	nonsense	probably null
R1997:Zan	UTSW	5	137,403,114 (GRCm38)	nonsense	probably null
R2008:Zan	UTSW	5	137,452,450 (GRCm38)	missense	unknown
R2035:Zan	UTSW	5	137,443,947 (GRCm38)	missense	unknown
R2153:Zan	UTSW	5	137,436,400 (GRCm38)	missense	unknown
R2154:Zan	UTSW	5	137,414,249 (GRCm38)	unclassified	probably benign
R2176:Zan	UTSW	5	137,421,848 (GRCm38)	missense	unknown
R2217:Zan	UTSW	5	137,410,306 (GRCm38)	utr 3 prime	probably benign
R2218:Zan	UTSW	5	137,410,306 (GRCm38)	utr 3 prime	probably benign
R2237:Zan	UTSW	5	137,457,837 (GRCm38)	nonsense	probably null
R2239:Zan	UTSW	5	137,457,837 (GRCm38)	nonsense	probably null
R2346:Zan	UTSW	5	137,421,867 (GRCm38)	missense	unknown
R2360:Zan	UTSW	5	137,396,126 (GRCm38)	missense	unknown
R2389:Zan	UTSW	5	137,476,380 (GRCm38)	critical splice donor site	probably null
R2412:Zan	UTSW	5	137,414,163 (GRCm38)	splice site	probably null
R2426:Zan	UTSW	5	137,388,992 (GRCm38)	missense	unknown
R2435:Zan	UTSW	5	137,438,574 (GRCm38)	missense	unknown
R2509:Zan	UTSW	5	137,456,586 (GRCm38)	missense	unknown
R3416:Zan	UTSW	5	137,435,720 (GRCm38)	missense	unknown
R3691:Zan	UTSW	5	137,420,019 (GRCm38)	missense	unknown
R3853:Zan	UTSW	5	137,474,064 (GRCm38)	missense	probably damaging	1.00
R4006:Zan	UTSW	5	137,463,939 (GRCm38)	missense	unknown
R4007:Zan	UTSW	5	137,463,939 (GRCm38)	missense	unknown
R4033:Zan	UTSW	5	137,437,860 (GRCm38)	nonsense	probably null
R4059:Zan	UTSW	5	137,436,820 (GRCm38)	missense	unknown
R4109:Zan	UTSW	5	137,458,619 (GRCm38)	missense	unknown
R4194:Zan	UTSW	5	137,463,555 (GRCm38)	missense	unknown
R4226:Zan	UTSW	5	137,423,978 (GRCm38)	missense	unknown
R4457:Zan	UTSW	5	137,411,516 (GRCm38)	missense	unknown
R4544:Zan	UTSW	5	137,383,834 (GRCm38)	missense	unknown
R4546:Zan	UTSW	5	137,383,834 (GRCm38)	missense	unknown
R4642:Zan	UTSW	5	137,464,188 (GRCm38)	missense	unknown
R4708:Zan	UTSW	5	137,446,712 (GRCm38)	missense	unknown
R4773:Zan	UTSW	5	137,436,313 (GRCm38)	splice site	probably benign
R4774:Zan	UTSW	5	137,389,019 (GRCm38)	missense	unknown
R4788:Zan	UTSW	5	137,442,113 (GRCm38)	missense	unknown
R4795:Zan	UTSW	5	137,380,850 (GRCm38)	nonsense	probably null
R4796:Zan	UTSW	5	137,380,850 (GRCm38)	nonsense	probably null
R4812:Zan	UTSW	5	137,456,285 (GRCm38)	missense	unknown
R4832:Zan	UTSW	5	137,393,161 (GRCm38)	missense	unknown
R4882:Zan	UTSW	5	137,438,448 (GRCm38)	missense	unknown
R4896:Zan	UTSW	5	137,386,456 (GRCm38)	missense	unknown
R4921:Zan	UTSW	5	137,408,370 (GRCm38)	unclassified	probably benign
R4943:Zan	UTSW	5	137,457,890 (GRCm38)	missense	unknown
R4978:Zan	UTSW	5	137,406,921 (GRCm38)	unclassified	probably benign
R5013:Zan	UTSW	5	137,383,837 (GRCm38)	missense	unknown
R5024:Zan	UTSW	5	137,461,893 (GRCm38)	nonsense	probably null
R5230:Zan	UTSW	5	137,454,078 (GRCm38)	missense	unknown
R5354:Zan	UTSW	5	137,380,788 (GRCm38)	unclassified	probably benign
R5380:Zan	UTSW	5	137,457,840 (GRCm38)	missense	unknown
R5394:Zan	UTSW	5	137,464,074 (GRCm38)	missense	unknown
R5394:Zan	UTSW	5	137,435,634 (GRCm38)	missense	unknown
R5435:Zan	UTSW	5	137,403,762 (GRCm38)	missense	unknown
R5441:Zan	UTSW	5	137,436,751 (GRCm38)	missense	unknown
R5447:Zan	UTSW	5	137,472,191 (GRCm38)	missense	probably damaging	1.00
R5455:Zan	UTSW	5	137,454,000 (GRCm38)	missense	unknown
R5495:Zan	UTSW	5	137,470,408 (GRCm38)	missense	probably damaging	1.00
R5496:Zan	UTSW	5	137,436,345 (GRCm38)	missense	unknown
R5523:Zan	UTSW	5	137,421,893 (GRCm38)	missense	unknown
R5534:Zan	UTSW	5	137,438,451 (GRCm38)	missense	unknown
R5572:Zan	UTSW	5	137,394,431 (GRCm38)	missense	unknown
R5576:Zan	UTSW	5	137,428,482 (GRCm38)	nonsense	probably null
R5587:Zan	UTSW	5	137,391,762 (GRCm38)	missense	unknown
R5593:Zan	UTSW	5	137,468,338 (GRCm38)	missense	possibly damaging	0.72
R5600:Zan	UTSW	5	137,386,971 (GRCm38)	missense	unknown
R5682:Zan	UTSW	5	137,414,259 (GRCm38)	nonsense	probably null
R5712:Zan	UTSW	5	137,400,098 (GRCm38)	missense	unknown
R5751:Zan	UTSW	5	137,410,161 (GRCm38)	splice site	probably null
R5782:Zan	UTSW	5	137,420,007 (GRCm38)	missense	unknown
R5835:Zan	UTSW	5	137,456,655 (GRCm38)	missense	unknown
R5846:Zan	UTSW	5	137,394,376 (GRCm38)	splice site	probably null
R5903:Zan	UTSW	5	137,442,134 (GRCm38)	missense	unknown
R5911:Zan	UTSW	5	137,457,912 (GRCm38)	missense	unknown
R5935:Zan	UTSW	5	137,443,930 (GRCm38)	missense	unknown
R5985:Zan	UTSW	5	137,446,037 (GRCm38)	splice site	probably null
R5995:Zan	UTSW	5	137,378,809 (GRCm38)	unclassified	probably benign
R6012:Zan	UTSW	5	137,464,529 (GRCm38)	missense	unknown
R6077:Zan	UTSW	5	137,414,297 (GRCm38)	unclassified	probably benign
R6227:Zan	UTSW	5	137,468,343 (GRCm38)	missense	probably damaging	0.96
R6262:Zan	UTSW	5	137,429,485 (GRCm38)	splice site	probably null
R6337:Zan	UTSW	5	137,452,488 (GRCm38)	missense	unknown
R6598:Zan	UTSW	5	137,406,364 (GRCm38)	unclassified	probably benign
R6725:Zan	UTSW	5	137,438,520 (GRCm38)	missense	unknown
R6765:Zan	UTSW	5	137,393,147 (GRCm38)	missense	unknown
R6820:Zan	UTSW	5	137,407,844 (GRCm38)	unclassified	probably benign
R6829:Zan	UTSW	5	137,416,278 (GRCm38)	unclassified	probably benign
R6851:Zan	UTSW	5	137,396,191 (GRCm38)	missense	unknown
R6903:Zan	UTSW	5	137,456,304 (GRCm38)	missense	unknown
R6910:Zan	UTSW	5	137,419,080 (GRCm38)	missense	unknown
R6968:Zan	UTSW	5	137,461,813 (GRCm38)	missense	unknown
R7021:Zan	UTSW	5	137,423,951 (GRCm38)	missense	unknown
R7039:Zan	UTSW	5	137,400,134 (GRCm38)	missense	unknown
R7101:Zan	UTSW	5	137,398,290 (GRCm38)	missense	unknown
R7102:Zan	UTSW	5	137,454,200 (GRCm38)	critical splice donor site	probably null
R7155:Zan	UTSW	5	137,461,844 (GRCm38)	missense	unknown
R7158:Zan	UTSW	5	137,400,644 (GRCm38)	missense	unknown
R7170:Zan	UTSW	5	137,463,494 (GRCm38)	missense	unknown
R7203:Zan	UTSW	5	137,434,096 (GRCm38)	missense	unknown
R7305:Zan	UTSW	5	137,415,139 (GRCm38)	missense	unknown
R7327:Zan	UTSW	5	137,465,232 (GRCm38)	missense	probably benign	0.35
R7340:Zan	UTSW	5	137,383,830 (GRCm38)	missense	unknown
R7360:Zan	UTSW	5	137,386,970 (GRCm38)	missense	unknown
R7385:Zan	UTSW	5	137,434,154 (GRCm38)	nonsense	probably null
R7385:Zan	UTSW	5	137,450,491 (GRCm38)	missense	unknown
R7438:Zan	UTSW	5	137,425,562 (GRCm38)	missense	unknown
R7453:Zan	UTSW	5	137,466,002 (GRCm38)	missense	probably damaging	1.00
R7483:Zan	UTSW	5	137,446,795 (GRCm38)	missense	unknown
R7499:Zan	UTSW	5	137,464,356 (GRCm38)	missense	probably benign	0.23
R7566:Zan	UTSW	5	137,412,583 (GRCm38)	critical splice donor site	probably null
R7641:Zan	UTSW	5	137,467,108 (GRCm38)	missense	possibly damaging	0.74
R7674:Zan	UTSW	5	137,467,108 (GRCm38)	missense	possibly damaging	0.74
R7678:Zan	UTSW	5	137,463,540 (GRCm38)	missense	unknown
R7785:Zan	UTSW	5	137,429,143 (GRCm38)	missense	unknown
R7814:Zan	UTSW	5	137,463,579 (GRCm38)	missense	unknown
R7841:Zan	UTSW	5	137,436,802 (GRCm38)	missense	unknown
R7861:Zan	UTSW	5	137,407,033 (GRCm38)	missense	unknown
R7869:Zan	UTSW	5	137,473,601 (GRCm38)	missense	probably damaging	1.00
R7933:Zan	UTSW	5	137,463,579 (GRCm38)	missense	unknown
R7934:Zan	UTSW	5	137,463,579 (GRCm38)	missense	unknown
R7935:Zan	UTSW	5	137,463,579 (GRCm38)	missense	unknown
R7960:Zan	UTSW	5	137,464,892 (GRCm38)	missense	unknown
R7960:Zan	UTSW	5	137,409,603 (GRCm38)	missense	unknown
R7990:Zan	UTSW	5	137,393,090 (GRCm38)	missense	unknown
R8008:Zan	UTSW	5	137,405,362 (GRCm38)	missense	unknown
R8025:Zan	UTSW	5	137,406,352 (GRCm38)	missense	unknown
R8061:Zan	UTSW	5	137,436,631 (GRCm38)	missense	unknown
R8190:Zan	UTSW	5	137,467,084 (GRCm38)	missense	probably damaging	1.00
R8202:Zan	UTSW	5	137,389,327 (GRCm38)	missense	unknown
R8302:Zan	UTSW	5	137,409,661 (GRCm38)	missense	unknown
R8305:Zan	UTSW	5	137,450,551 (GRCm38)	missense	unknown
R8328:Zan	UTSW	5	137,394,464 (GRCm38)	missense	unknown
R8377:Zan	UTSW	5	137,391,687 (GRCm38)	missense	unknown
R8404:Zan	UTSW	5	137,398,332 (GRCm38)	missense	unknown
R8502:Zan	UTSW	5	137,473,583 (GRCm38)	missense	probably damaging	1.00
R8510:Zan	UTSW	5	137,388,938 (GRCm38)	missense	unknown
R8511:Zan	UTSW	5	137,446,846 (GRCm38)	missense	unknown
R8527:Zan	UTSW	5	137,435,709 (GRCm38)	missense	unknown
R8695:Zan	UTSW	5	137,386,955 (GRCm38)	missense	unknown
R8708:Zan	UTSW	5	137,463,277 (GRCm38)	critical splice donor site	probably null
R8744:Zan	UTSW	5	137,427,864 (GRCm38)	missense	unknown
R8795:Zan	UTSW	5	137,398,260 (GRCm38)	missense	unknown
R8841:Zan	UTSW	5	137,456,674 (GRCm38)	missense	unknown
R8862:Zan	UTSW	5	137,474,412 (GRCm38)	missense	probably benign	0.28
R8937:Zan	UTSW	5	137,395,626 (GRCm38)	missense	unknown
R8973:Zan	UTSW	5	137,389,316 (GRCm38)	missense	unknown
R8988:Zan	UTSW	5	137,408,301 (GRCm38)	missense	unknown
R8995:Zan	UTSW	5	137,395,620 (GRCm38)	missense	unknown
R9036:Zan	UTSW	5	137,465,944 (GRCm38)	missense	probably damaging	1.00
R9037:Zan	UTSW	5	137,454,316 (GRCm38)	missense	unknown
R9061:Zan	UTSW	5	137,464,391 (GRCm38)	missense	probably damaging	0.98
R9077:Zan	UTSW	5	137,403,206 (GRCm38)	missense	unknown
R9164:Zan	UTSW	5	137,424,071 (GRCm38)	missense	unknown
R9186:Zan	UTSW	5	137,393,548 (GRCm38)	missense	unknown
R9222:Zan	UTSW	5	137,467,201 (GRCm38)	missense	possibly damaging	0.56
R9224:Zan	UTSW	5	137,474,007 (GRCm38)	missense	probably damaging	1.00
R9277:Zan	UTSW	5	137,463,992 (GRCm38)	missense	unknown
R9296:Zan	UTSW	5	137,388,876 (GRCm38)	missense	unknown
R9300:Zan	UTSW	5	137,470,257 (GRCm38)	critical splice donor site	probably null
R9352:Zan	UTSW	5	137,436,483 (GRCm38)	missense	unknown
R9382:Zan	UTSW	5	137,391,655 (GRCm38)	missense	unknown
R9393:Zan	UTSW	5	137,405,420 (GRCm38)	missense	unknown
R9534:Zan	UTSW	5	137,409,683 (GRCm38)	nonsense	probably null
R9548:Zan	UTSW	5	137,403,061 (GRCm38)	missense	unknown
R9564:Zan	UTSW	5	137,406,426 (GRCm38)	missense	unknown
R9623:Zan	UTSW	5	137,463,374 (GRCm38)	missense	unknown
R9643:Zan	UTSW	5	137,458,550 (GRCm38)	missense	unknown
R9648:Zan	UTSW	5	137,407,730 (GRCm38)	missense	unknown
R9663:Zan	UTSW	5	137,380,857 (GRCm38)	missense	unknown
R9683:Zan	UTSW	5	137,464,514 (GRCm38)	missense	unknown
R9688:Zan	UTSW	5	137,468,455 (GRCm38)	missense	probably damaging	1.00
R9700:Zan	UTSW	5	137,456,574 (GRCm38)	missense	unknown
R9715:Zan	UTSW	5	137,400,555 (GRCm38)	missense	unknown
R9722:Zan	UTSW	5	137,389,062 (GRCm38)	missense	unknown
RF013:Zan	UTSW	5	137,391,720 (GRCm38)	missense	unknown
X0062:Zan	UTSW	5	137,446,238 (GRCm38)	missense	unknown
X0066:Zan	UTSW	5	137,464,430 (GRCm38)	missense	probably benign	0.00
Z1176:Zan	UTSW	5	137,411,588 (GRCm38)	missense	unknown
Z1176:Zan	UTSW	5	137,398,362 (GRCm38)	missense	unknown
Z1176:Zan	UTSW	5	137,393,597 (GRCm38)	missense	unknown
Z1177:Zan	UTSW	5	137,393,105 (GRCm38)	missense	unknown
Z1177:Zan	UTSW	5	137,389,061 (GRCm38)	missense	unknown
Z1177:Zan	UTSW	5	137,383,733 (GRCm38)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- AACCACCCTGGGTATGTTGG -3'
(R):5'- TCTCAGTGTCAGTCTCTAAGTGG -3'

Sequencing Primer
(F):5'- GTATGTTGGCAGCCACACTC -3'
(R):5'- CTTGGCATATAGTCCACTGTCAGAG -3'

Posted On

2019-06-26