Mutagenetix > Incidental Mutation

Incidental Mutation 'R7213:Vmn2r53'

561205

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r53

Ensembl Gene

ENSMUSG00000096002

Gene Name

vomeronasal 2, receptor 53

Synonyms

EG637908

MMRRC Submission

045341-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.086) question?

Stock #

R7213 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

12581470-12606544 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 12601056 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Glycine at position 226 (S226G)

Ref Sequence

ENSEMBL: ENSMUSP00000126979 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170412]

AlphaFold

A0A3B2W4A7

Predicted Effect

probably benign
Transcript: ENSMUST00000170412
AA Change: S226G

PolyPhen 2 Score 0.171 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000126979
Gene: ENSMUSG00000096002
AA Change: S226G

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	5	397	3.6e-58	PFAM
Pfam:NCD3G	442	495	2.2e-19	PFAM
Pfam:7tm_3	526	763	3.1e-53	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

99% (80/81)

Allele List at MGI

Other mutations in this stock

Total: 80 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2010315B03Rik	G	T	9: 124,293,900 (GRCm38)	Y131*	probably null	Het
2310009B15Rik	A	T	1: 138,853,629 (GRCm38)	V94D	probably damaging	Het
Actr1b	T	A	1: 36,702,140 (GRCm38)	N120I	probably damaging	Het
Adam17	A	T	12: 21,336,678 (GRCm38)	Y452*	probably null	Het
Adam19	A	T	11: 46,121,471 (GRCm38)	T265S	probably benign	Het
Adgrb1	A	G	15: 74,569,884 (GRCm38)	T945A	probably benign	Het
BC067074	T	A	13: 113,317,941 (GRCm38)	F174I		Het
Bpifa5	G	A	2: 154,165,983 (GRCm38)	V182M	possibly damaging	Het
Celsr3	C	T	9: 108,849,040 (GRCm38)	T3156I	probably damaging	Het
Cntrl	A	G	2: 35,135,680 (GRCm38)	M674V	possibly damaging	Het
Cry2	C	T	2: 92,413,659 (GRCm38)	V390I	probably benign	Het
Cwc25	G	T	11: 97,754,029 (GRCm38)	Q168K	probably benign	Het
Dcbld2	C	T	16: 58,450,763 (GRCm38)	A301V	probably benign	Het
Dclre1a	T	C	19: 56,529,635 (GRCm38)	Y1004C	probably damaging	Het
Ddhd1	A	C	14: 45,657,753 (GRCm38)	S87A	probably benign	Het
Ear1	A	G	14: 43,819,154 (GRCm38)	C86R	probably damaging	Het
Epha5	T	C	5: 84,233,923 (GRCm38)		probably null	Het
Fat2	A	T	11: 55,281,045 (GRCm38)	Y2947*	probably null	Het
Fat4	G	A	3: 38,999,087 (GRCm38)	V4077M	possibly damaging	Het
Fbxl12	A	T	9: 20,639,008 (GRCm38)	V140E	probably damaging	Het
Fbxo16	A	G	14: 65,299,419 (GRCm38)		probably null	Het
Fto	A	T	8: 91,391,507 (GRCm38)	Q29L	probably benign	Het
Gk5	C	T	9: 96,145,712 (GRCm38)	T200M	probably damaging	Het
Gm5916	C	T	9: 36,128,650 (GRCm38)	G14E	possibly damaging	Het
Gm9972	A	T	11: 43,036,408 (GRCm38)		probably benign	Het
Gpr139	T	A	7: 119,145,099 (GRCm38)	M88L	probably benign	Het
Gpr26	T	C	7: 131,967,490 (GRCm38)	L188P	probably damaging	Het
Hbp1	A	T	12: 31,937,197 (GRCm38)	S219T	probably benign	Het
Hist1h2ab	G	T	13: 23,751,163 (GRCm38)	A11S	unknown	Het
Hr	A	T	14: 70,558,350 (GRCm38)	E445V	probably damaging	Het
Il12rb1	A	G	8: 70,816,453 (GRCm38)	K426E	probably benign	Het
Itgbl1	G	A	14: 123,973,297 (GRCm38)	C469Y	probably damaging	Het
Kdm2b	A	T	5: 122,921,469 (GRCm38)	N523K	probably damaging	Het
Kptn	A	G	7: 16,120,779 (GRCm38)	N125S	possibly damaging	Het
Krt17	T	C	11: 100,258,530 (GRCm38)	N238S	probably benign	Het
Lemd3	G	A	10: 120,978,240 (GRCm38)	R363*	probably null	Het
Mmrn1	G	A	6: 60,944,543 (GRCm38)		probably benign	Het
Mtus1	T	C	8: 41,084,487 (GRCm38)	D64G	probably damaging	Het
Muc16	A	G	9: 18,641,416 (GRCm38)	V4527A	probably benign	Het
Naip2	T	A	13: 100,187,483 (GRCm38)	D193V	probably damaging	Het
Nbeal1	G	C	1: 60,237,151 (GRCm38)	V684L	probably benign	Het
Nf1	A	G	11: 79,469,819 (GRCm38)	H1462R	probably benign	Het
Olfr133	T	C	17: 38,149,074 (GRCm38)	V162A	probably benign	Het
Olfr988	A	T	2: 85,353,556 (GRCm38)	Y123*	probably null	Het
Pappa2	T	A	1: 158,936,886 (GRCm38)	T352S	possibly damaging	Het
Pcnt	A	G	10: 76,408,904 (GRCm38)	L1114P	probably damaging	Het
Pde6b	T	C	5: 108,404,090 (GRCm38)	Y212H	probably damaging	Het
Pik3c2g	A	T	6: 139,860,264 (GRCm38)	I604F		Het
Pld2	A	G	11: 70,553,372 (GRCm38)	D498G	probably benign	Het
Prag1	A	T	8: 36,146,615 (GRCm38)	Q1107L	probably damaging	Het
Pwp1	A	T	10: 85,876,309 (GRCm38)	I110F	probably benign	Het
Rims4	C	T	2: 163,864,061 (GRCm38)	V218I	probably benign	Het
Rnf10	T	C	5: 115,242,474 (GRCm38)	S754G	probably damaging	Het
Rnf10	C	T	5: 115,242,473 (GRCm38)	S754N	probably damaging	Het
Sel1l2	A	T	2: 140,244,135 (GRCm38)	V512E	probably damaging	Het
Shank2	T	A	7: 144,031,409 (GRCm38)	M49K	probably benign	Het
Sipa1	T	C	19: 5,660,523 (GRCm38)	D153G	probably damaging	Het
Slc46a2	C	T	4: 59,914,279 (GRCm38)	V215I	possibly damaging	Het
Slco6c1	G	A	1: 97,127,946 (GRCm38)	L77F	probably benign	Het
Smarca2	T	C	19: 26,647,131 (GRCm38)	L397P	possibly damaging	Het
Spg7	C	T	8: 123,090,232 (GRCm38)	A554V	probably damaging	Het
Stab1	T	A	14: 31,143,673 (GRCm38)	M1753L	probably benign	Het
Stk11	A	G	10: 80,116,618 (GRCm38)	M1V	probably null	Het
Supt6	C	A	11: 78,232,150 (GRCm38)	G136C	probably damaging	Het
Svil	G	T	18: 5,094,574 (GRCm38)	R1418L	probably damaging	Het
Tfg	A	G	16: 56,701,153 (GRCm38)	S167P	probably benign	Het
Timeless	T	C	10: 128,243,289 (GRCm38)	V335A	probably benign	Het
Tll2	C	A	19: 41,120,227 (GRCm38)	R328L	probably damaging	Het
Tomm7	T	C	5: 23,844,061 (GRCm38)	S5G	possibly damaging	Het
Ttc39c	G	A	18: 12,687,081 (GRCm38)		probably null	Het
Ttn	T	C	2: 76,725,761 (GRCm38)	E30300G	probably benign	Het
Tuba4a	A	T	1: 75,215,697 (GRCm38)	D452E	possibly damaging	Het
Tubgcp2	T	C	7: 140,008,014 (GRCm38)	I233V	probably benign	Het
Tubgcp5	T	A	7: 55,806,112 (GRCm38)	V296E	probably damaging	Het
Xrcc6	T	C	15: 82,016,826 (GRCm38)		probably benign	Het
Zfhx4	C	A	3: 5,396,644 (GRCm38)	D1192E	probably benign	Het
Zfp180	G	T	7: 24,104,513 (GRCm38)	W119L	possibly damaging	Het
Zfp3	A	G	11: 70,772,525 (GRCm38)	I437V	probably benign	Het
Zfp616	A	T	11: 74,085,863 (GRCm38)	Q986L	probably benign	Het
Zfp937	T	A	2: 150,239,465 (GRCm38)	C472S	probably damaging	Het

Other mutations in Vmn2r53

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01090:Vmn2r53	APN	7	12,600,908 (GRCm38)	missense	possibly damaging	0.70
IGL01997:Vmn2r53	APN	7	12,582,446 (GRCm38)	missense	possibly damaging	0.54
IGL02442:Vmn2r53	APN	7	12,581,729 (GRCm38)	missense	probably damaging	1.00
IGL02449:Vmn2r53	APN	7	12,582,361 (GRCm38)	missense	probably damaging	1.00
IGL02589:Vmn2r53	APN	7	12,581,945 (GRCm38)	missense	possibly damaging	0.93
IGL02986:Vmn2r53	APN	7	12,581,466 (GRCm38)	unclassified	probably benign
IGL03064:Vmn2r53	APN	7	12,601,010 (GRCm38)	missense	possibly damaging	0.89
IGL03093:Vmn2r53	APN	7	12,600,864 (GRCm38)	missense	probably benign	0.03
IGL03244:Vmn2r53	APN	7	12,606,508 (GRCm38)	missense	probably damaging	1.00
IGL03252:Vmn2r53	APN	7	12,606,391 (GRCm38)	missense	probably damaging	1.00
IGL03264:Vmn2r53	APN	7	12,581,892 (GRCm38)	missense	possibly damaging	0.95
IGL03293:Vmn2r53	APN	7	12,598,422 (GRCm38)	missense	probably benign	0.34
R0109:Vmn2r53	UTSW	7	12,582,066 (GRCm38)	missense	probably damaging	1.00
R0453:Vmn2r53	UTSW	7	12,582,411 (GRCm38)	missense	probably damaging	1.00
R0735:Vmn2r53	UTSW	7	12,581,780 (GRCm38)	missense	probably benign
R0881:Vmn2r53	UTSW	7	12,600,932 (GRCm38)	missense	probably benign	0.01
R0894:Vmn2r53	UTSW	7	12,601,214 (GRCm38)	missense	probably benign	0.00
R0973:Vmn2r53	UTSW	7	12,601,392 (GRCm38)	missense	probably damaging	1.00
R0973:Vmn2r53	UTSW	7	12,601,392 (GRCm38)	missense	probably damaging	1.00
R0974:Vmn2r53	UTSW	7	12,601,392 (GRCm38)	missense	probably damaging	1.00
R0990:Vmn2r53	UTSW	7	12,581,502 (GRCm38)	missense	probably benign
R1102:Vmn2r53	UTSW	7	12,598,483 (GRCm38)	missense	possibly damaging	0.94
R1141:Vmn2r53	UTSW	7	12,600,746 (GRCm38)	missense	possibly damaging	0.54
R1263:Vmn2r53	UTSW	7	12,581,606 (GRCm38)	missense	probably benign	0.41
R1343:Vmn2r53	UTSW	7	12,584,774 (GRCm38)	missense	probably benign	0.08
R1750:Vmn2r53	UTSW	7	12,581,705 (GRCm38)	missense	probably damaging	1.00
R1836:Vmn2r53	UTSW	7	12,600,885 (GRCm38)	missense	probably damaging	1.00
R2035:Vmn2r53	UTSW	7	12,598,511 (GRCm38)	missense	possibly damaging	0.76
R2202:Vmn2r53	UTSW	7	12,601,439 (GRCm38)	missense	probably damaging	1.00
R3707:Vmn2r53	UTSW	7	12,582,054 (GRCm38)	missense	possibly damaging	0.95
R4372:Vmn2r53	UTSW	7	12,581,729 (GRCm38)	missense	probably damaging	0.98
R4615:Vmn2r53	UTSW	7	12,582,302 (GRCm38)	missense	probably damaging	1.00
R4655:Vmn2r53	UTSW	7	12,582,005 (GRCm38)	missense	possibly damaging	0.83
R4663:Vmn2r53	UTSW	7	12,600,974 (GRCm38)	missense	probably benign	0.21
R4708:Vmn2r53	UTSW	7	12,601,202 (GRCm38)	missense	probably benign
R4710:Vmn2r53	UTSW	7	12,601,202 (GRCm38)	missense	probably benign
R4774:Vmn2r53	UTSW	7	12,600,765 (GRCm38)	nonsense	probably null
R4859:Vmn2r53	UTSW	7	12,601,403 (GRCm38)	missense	probably damaging	1.00
R5061:Vmn2r53	UTSW	7	12,581,814 (GRCm38)	missense	probably benign	0.01
R5561:Vmn2r53	UTSW	7	12,601,420 (GRCm38)	missense	probably damaging	1.00
R5729:Vmn2r53	UTSW	7	12,600,806 (GRCm38)	missense	probably damaging	1.00
R6004:Vmn2r53	UTSW	7	12,582,401 (GRCm38)	missense	probably benign	0.12
R6083:Vmn2r53	UTSW	7	12,581,881 (GRCm38)	missense	probably benign
R6312:Vmn2r53	UTSW	7	12,598,639 (GRCm38)	critical splice acceptor site	probably null
R6700:Vmn2r53	UTSW	7	12,581,706 (GRCm38)	missense	probably damaging	0.96
R6783:Vmn2r53	UTSW	7	12,601,433 (GRCm38)	missense	probably damaging	1.00
R6852:Vmn2r53	UTSW	7	12,606,514 (GRCm38)	missense	probably damaging	0.99
R6889:Vmn2r53	UTSW	7	12,601,142 (GRCm38)	missense	probably benign	0.10
R6940:Vmn2r53	UTSW	7	12,582,416 (GRCm38)	missense	probably benign	0.19
R7100:Vmn2r53	UTSW	7	12,581,586 (GRCm38)	nonsense	probably null
R7174:Vmn2r53	UTSW	7	12,581,701 (GRCm38)	missense	probably benign	0.01
R7276:Vmn2r53	UTSW	7	12,606,432 (GRCm38)	missense	probably damaging	0.99
R7515:Vmn2r53	UTSW	7	12,581,919 (GRCm38)	missense	probably benign	0.05
R7678:Vmn2r53	UTSW	7	12,598,498 (GRCm38)	missense	probably benign	0.04
R7714:Vmn2r53	UTSW	7	12,606,491 (GRCm38)	missense	probably damaging	1.00
R7843:Vmn2r53	UTSW	7	12,582,099 (GRCm38)	missense	probably damaging	1.00
R8208:Vmn2r53	UTSW	7	12,601,395 (GRCm38)	missense	probably damaging	1.00
R8211:Vmn2r53	UTSW	7	12,581,916 (GRCm38)	missense	probably benign	0.01
R8478:Vmn2r53	UTSW	7	12,606,354 (GRCm38)	missense	probably benign	0.01
R8853:Vmn2r53	UTSW	7	12,581,810 (GRCm38)	missense	probably damaging	1.00
R8924:Vmn2r53	UTSW	7	12,600,825 (GRCm38)	missense	probably benign	0.17
R8963:Vmn2r53	UTSW	7	12,581,999 (GRCm38)	missense	probably damaging	1.00
R9042:Vmn2r53	UTSW	7	12,581,508 (GRCm38)	missense	probably benign
R9076:Vmn2r53	UTSW	7	12,606,304 (GRCm38)	missense	probably damaging	1.00
R9407:Vmn2r53	UTSW	7	12,601,197 (GRCm38)	missense	probably damaging	0.99
R9690:Vmn2r53	UTSW	7	12,581,985 (GRCm38)	missense	probably damaging	1.00
Z1176:Vmn2r53	UTSW	7	12,601,304 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GCCATGTACAATCAAAGGTGAAC -3'
(R):5'- CACACAGATGTTGACCCCAG -3'

Sequencing Primer
(F):5'- TGTACAATCAAAGGTGAACTCCCAG -3'
(R):5'- AGATGTTGACCCCAGCTGGTG -3'

Posted On

2019-06-26