Mutagenetix > Incidental Mutation

Incidental Mutation 'R7214:Olfr1474'

561353

Institutional Source

Beutler Lab

Gene Symbol

Olfr1474

Ensembl Gene

ENSMUSG00000096273

Gene Name

olfactory receptor 1474

Synonyms

MOR202-42, MOR202-26P, GA_x6K02T2RE5P-3803583-3804527

MMRRC Submission

Accession Numbers

Genbank: NM_001011842.1

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R7214 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

13469565-13472157 bp(+) (GRCm38)

Type of Mutation

start codon destroyed

DNA Base Change (assembly)

T to A at 13470973 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 1 (M1K)

Ref Sequence

ENSEMBL: ENSMUSP00000151810 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000096202] [ENSMUST00000207529] [ENSMUST00000220113]

AlphaFold

Q7TQQ8

Predicted Effect

probably null
Transcript: ENSMUST00000096202
AA Change: M1K

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000093916
Gene: ENSMUSG00000096273
AA Change: M1K

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	306	1.6e-52	PFAM
Pfam:7TM_GPCR_Srsx	33	303	1e-7	PFAM
Pfam:7tm_1	39	288	8.7e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000207529

Predicted Effect

probably null
Transcript: ENSMUST00000220113
AA Change: M1K

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

none

Other mutations in this stock

Total: 94 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrg5	C	T	8: 94,934,018	T95I		Het
Arap2	C	T	5: 62,749,338	V113I	probably benign	Het
Arntl	T	A	7: 113,299,403	I346K	probably benign	Het
Atraid	C	T	5: 31,052,246	Q85*	probably null	Het
BC005561	A	G	5: 104,522,363	T1584A	probably benign	Het
Bcdin3d	T	C	15: 99,470,463	D285G	probably benign	Het
Carmil3	T	C	14: 55,498,612	W604R	probably damaging	Het
Ccl6	C	T	11: 83,589,756		probably null	Het
Cct4	C	T	11: 22,990,616		probably benign	Het
Cd2ap	C	T	17: 42,845,394	R84Q	possibly damaging	Het
Cdt1	T	A	8: 122,568,273		probably null	Het
Cln3	C	T	7: 126,582,770	G40D	probably damaging	Het
Crlf3	T	C	11: 80,064,390	S47G	possibly damaging	Het
Csrp3	T	C	7: 48,830,637	K193R	probably benign	Het
Cxcl3	A	C	5: 90,786,360	E33A	probably damaging	Het
Cyp11b1	T	C	15: 74,836,859	D362G	probably benign	Het
Dnah2	T	C	11: 69,431,109	D3795G	probably damaging	Het
Dnah3	G	A	7: 119,922,742	A4076V	probably damaging	Het
Dnaja3	T	A	16: 4,701,182	I380N	possibly damaging	Het
Dock10	T	A	1: 80,568,529	H785L	probably benign	Het
Dopey2	T	G	16: 93,810,135	F2226V	possibly damaging	Het
Dscaml1	A	T	9: 45,670,139	I419F	probably benign	Het
Dyrk4	T	A	6: 126,885,237	I431F	probably benign	Het
E430018J23Rik	A	G	7: 127,391,523	S431P	probably benign	Het
Endog	C	T	2: 30,172,890	R181C	probably damaging	Het
Fam90a1a	A	T	8: 21,963,625	Y332F	probably benign	Het
Fastkd3	T	C	13: 68,589,380	I588T	probably benign	Het
Galnt9	G	A	5: 110,589,828	V217I	probably benign	Het
Gm973	A	T	1: 59,562,729	R501*	probably null	Het
Gnptab	C	T	10: 88,379,157		probably benign	Het
Gpn1	T	C	5: 31,503,417	F184S	probably damaging	Het
Imp3	G	T	9: 56,937,723	V73L	probably benign	Het
Ipo11	T	C	13: 106,895,857	D259G	probably null	Het
Jag1	T	C	2: 137,106,882	S142G	probably benign	Het
Klhl5	A	T	5: 65,131,755	E120V	probably benign	Het
Lct	C	A	1: 128,300,460	V1099L	probably benign	Het
Lrba	G	C	3: 86,328,326	W912C	probably damaging	Het
Lss	C	T	10: 76,547,471	T535I	probably damaging	Het
Mcts2	T	C	2: 152,687,377	I36T	probably benign	Het
Meikin	T	A	11: 54,411,912	N383K	probably benign	Het
Myo1g	T	C	11: 6,511,055	Y663C	probably damaging	Het
Nadk2	T	A	15: 9,108,254	M419K	probably damaging	Het
Nbeal1	G	C	1: 60,237,151	V684L	probably benign	Het
Nfat5	T	C	8: 107,293,883	S20P	probably damaging	Het
Nlrp1a	C	T	11: 71,123,293	C377Y	probably damaging	Het
Nlrp9a	T	C	7: 26,551,038	V76A	probably damaging	Het
Nrap	C	A	19: 56,378,135	A341S	probably benign	Het
Ntng2	T	C	2: 29,227,720	S239G	probably damaging	Het
Nup188	T	A	2: 30,307,554	C207S	possibly damaging	Het
Olfr1077-ps1	C	T	2: 86,525,806	V124I	probably damaging	Het
Olfr1328	T	A	4: 118,933,949	I300F	possibly damaging	Het
Olfr17	T	C	7: 107,098,412	*316R	probably null	Het
Olfr346	T	C	2: 36,688,095	I31T	probably benign	Het
Olfr705	T	C	7: 106,874,267		probably benign	Het
Olfr891	A	C	9: 38,180,022	V267G	probably damaging	Het
Pcdhgb2	C	A	18: 37,690,106	A50E	probably damaging	Het
Pgap1	A	T	1: 54,543,061	M209K	possibly damaging	Het
Plbd1	T	A	6: 136,612,831	D463V	probably damaging	Het
Plcg2	T	A	8: 117,583,549	I380N	probably damaging	Het
Pnpt1	T	C	11: 29,137,285	W184R	probably damaging	Het
Pptc7	G	A	5: 122,313,777	V202I	probably benign	Het
Prpf6	C	T	2: 181,640,596	A510V	probably damaging	Het
Prr5l	T	C	2: 101,729,432	Y235C	probably benign	Het
Ptprk	T	C	10: 28,574,909	V1022A	probably benign	Het
Rbl2	T	C	8: 91,083,429		probably null	Het
Rmnd1	T	C	10: 4,410,753	K348E	probably benign	Het
Rsf1	CGGCGGC	CGGCGGCGGGGGCGGC	7: 97,579,929		probably benign	Het
Sacs	T	A	14: 61,191,792	N433K	probably benign	Het
Scyl1	T	C	19: 5,760,029	T590A	probably benign	Het
Sdhd	A	G	9: 50,597,233	V111A	possibly damaging	Het
Sec24b	G	T	3: 130,033,860	P330Q	probably benign	Het
Serpinb6d	C	T	13: 33,664,145	P31S	probably damaging	Het
Slc26a9	A	T	1: 131,759,473	R457W	probably damaging	Het
Slc5a8	G	A	10: 88,919,502	M490I	probably benign	Het
Slx4	A	G	16: 3,988,980	I533T	probably benign	Het
Slx4ip	T	A	2: 137,046,730	F110L	probably benign	Het
Smchd1	T	C	17: 71,345,364	R2000G	probably benign	Het
Snx8	T	C	5: 140,360,253	E75G	possibly damaging	Het
Stab2	C	A	10: 86,899,841	C1292F	probably damaging	Het
Swt1	A	T	1: 151,394,613	M617K	possibly damaging	Het
Tiam2	T	A	17: 3,518,412	I1611N	possibly damaging	Het
Tmem63b	T	G	17: 45,661,822	N682T	probably benign	Het
Trio	C	T	15: 27,871,187	V674M	probably damaging	Het
Trpc3	G	A	3: 36,650,137	T557M	possibly damaging	Het
Vac14	T	G	8: 110,671,042	L463R	probably damaging	Het
Vmn2r43	C	T	7: 8,253,380		probably null	Het
Wdr49	A	T	3: 75,358,444	Y232N	possibly damaging	Het
Wdr90	T	C	17: 25,845,393	M1835V	probably benign	Het
Xpc	T	C	6: 91,492,338	E809G	probably damaging	Het
Zfhx3	A	T	8: 108,948,861	Q2181L	probably damaging	Het
Zfp442	T	C	2: 150,409,281	T234A	probably benign	Het
Zfp764	A	T	7: 127,405,278	M227K	probably benign	Het
Zfp957	A	G	14: 79,213,310	S350P	unknown	Het
Zscan29	T	A	2: 121,169,280	K147*	probably null	Het

Other mutations in Olfr1474

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03256:Olfr1474	APN	19	13471267	missense	probably damaging	0.99
D605:Olfr1474	UTSW	19	13471157	nonsense	probably null
R0173:Olfr1474	UTSW	19	13471701	missense	probably benign	0.02
R1102:Olfr1474	UTSW	19	13471407	missense	probably damaging	0.97
R1515:Olfr1474	UTSW	19	13471680	missense	probably damaging	0.97
R1780:Olfr1474	UTSW	19	13471362	missense	probably benign	0.14
R2061:Olfr1474	UTSW	19	13471241	missense	probably damaging	0.98
R4016:Olfr1474	UTSW	19	13471197	missense	possibly damaging	0.95
R4485:Olfr1474	UTSW	19	13471555	missense	probably benign	0.08
R5119:Olfr1474	UTSW	19	13471546	missense	probably benign	0.00
R5150:Olfr1474	UTSW	19	13471430	missense	probably benign	0.01
R5156:Olfr1474	UTSW	19	13471673	missense	probably damaging	1.00
R5699:Olfr1474	UTSW	19	13470972	start codon destroyed	probably null	0.78
R5800:Olfr1474	UTSW	19	13471896	missense	probably benign	0.06
R5840:Olfr1474	UTSW	19	13471878	missense	probably benign	0.01
R5953:Olfr1474	UTSW	19	13471368	missense	possibly damaging	0.92
R5997:Olfr1474	UTSW	19	13471506	missense	probably benign	0.12
R6233:Olfr1474	UTSW	19	13471740	missense	probably damaging	1.00
R6488:Olfr1474	UTSW	19	13471617	missense	probably damaging	1.00
R6847:Olfr1474	UTSW	19	13471038	missense	probably benign	0.03
R6964:Olfr1474	UTSW	19	13471361	nonsense	probably null
R8001:Olfr1474	UTSW	19	13471422	missense	probably benign	0.03
R8035:Olfr1474	UTSW	19	13471899	missense	probably benign
R8129:Olfr1474	UTSW	19	13471144	missense	probably damaging	1.00
R9018:Olfr1474	UTSW	19	13471357	missense	possibly damaging	0.60
R9061:Olfr1474	UTSW	19	13471159	missense	probably damaging	0.98
R9065:Olfr1474	UTSW	19	13471306	missense	probably damaging	0.97
R9373:Olfr1474	UTSW	19	13471852	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGTTGTATTAGGCACCCCTGG -3'
(R):5'- CACCACCGAGAGATTACAGAGG -3'

Sequencing Primer
(F):5'- GCACCCCTGGTATCATATATTCTGAG -3'
(R):5'- CCGAGAGATTACAGAGGAAAAAGTAC -3'

Posted On

2019-06-26