Mutagenetix > Incidental Mutation

Incidental Mutation 'R7215:Olfr918'

561396

Institutional Source

Beutler Lab

Gene Symbol

Olfr918

Ensembl Gene

ENSMUSG00000046150

Gene Name

olfactory receptor 918

Synonyms

GA_x6K02T2PVTD-32375756-32374818, MOR164-3

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.242) question?

Stock #

R7215 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

38669861-38674601 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 38673447 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 12 (I12T)

Ref Sequence

ENSEMBL: ENSMUSP00000057210 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055099] [ENSMUST00000215461]

AlphaFold

E9PVZ7

Predicted Effect

probably benign
Transcript: ENSMUST00000055099
AA Change: I12T

PolyPhen 2 Score 0.053 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000057210
Gene: ENSMUSG00000046150
AA Change: I12T

Domain	Start	End	E-Value	Type
transmembrane domain	9	31	N/A	INTRINSIC
Pfam:7tm_4	44	319	6.5e-48	PFAM
Pfam:7tm_1	54	301	3.9e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215461

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

98% (79/81)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 82 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210408I21Rik	G	A	13: 77,323,571	V1032M	possibly damaging	Het
Abca13	T	A	11: 9,288,405		probably null	Het
Adamts14	T	A	10: 61,211,596	H739L	possibly damaging	Het
Adgrl3	A	G	5: 81,693,550	E758G	probably damaging	Het
Ano3	T	A	2: 110,665,932	T826S	probably damaging	Het
Arhgap45	C	T	10: 80,025,482	T493I	possibly damaging	Het
Atg9b	A	C	5: 24,388,041	W455G	probably damaging	Het
Atp4a	A	G	7: 30,717,360	N496S	possibly damaging	Het
Bckdk	T	A	7: 127,905,110	D60E	possibly damaging	Het
Blmh	A	T	11: 76,965,899	K244*	probably null	Het
Btbd17	T	C	11: 114,791,465	I474V	possibly damaging	Het
C87436	A	G	6: 86,462,680	E451G	possibly damaging	Het
Camta1	T	C	4: 151,144,737	E546G	probably damaging	Het
Casp1	A	G	9: 5,298,523		probably null	Het
Ccdc114	C	T	7: 45,936,622	R148C	probably damaging	Het
Ccdc116	A	G	16: 17,139,928	Y456H	probably damaging	Het
Cep350	A	C	1: 155,894,707	S1812R	possibly damaging	Het
Chrna10	A	G	7: 102,112,208	L392P	possibly damaging	Het
Col22a1	A	T	15: 71,970,332	C434*	probably null	Het
Cxcl9	G	A	5: 92,323,888	Q98*	probably null	Het
Cyp2c54	G	A	19: 40,046,182	T348I	probably damaging	Het
Dnah7a	G	A	1: 53,618,350	R756C	probably damaging	Het
Dnajc18	T	C	18: 35,681,981	T239A	probably benign	Het
Dnase2a	A	T	8: 84,909,770		probably null	Het
Dpyd	A	G	3: 119,266,032	T793A	probably benign	Het
E430018J23Rik	A	G	7: 127,391,523	S431P	probably benign	Het
Edil3	T	C	13: 88,822,050		probably null	Het
Ehd1	T	A	19: 6,297,642	I342N	possibly damaging	Het
Erbb4	A	T	1: 68,339,460	S341T	probably benign	Het
Ezh1	T	A	11: 101,215,299	T87S	probably benign	Het
Fam20b	A	T	1: 156,690,553	W224R	probably damaging	Het
Galns	A	T	8: 122,599,348		probably null	Het
Gm13283	C	T	4: 88,760,730		probably benign	Het
Gm15448	C	T	7: 3,822,311	C444Y	unknown	Het
Gm49342	A	T	14: 50,944,583	M23L	probably benign	Het
Gm5114	T	A	7: 39,411,371	H18L	probably benign	Het
Gpr89	A	G	3: 96,880,088	W299R	probably damaging	Het
Hadha	G	T	5: 30,119,842	N755K	probably benign	Het
Inpp5d	A	T	1: 87,701,218	H620L	probably benign	Het
Klk1b3	T	A	7: 44,200,404		probably null	Het
Macf1	T	C	4: 123,507,304	T663A	probably damaging	Het
Man1b1	A	G	2: 25,350,390	N601S	probably benign	Het
Mbtps1	A	G	8: 119,524,568	V605A	possibly damaging	Het
Med23	C	G	10: 24,888,429	D311E	probably benign	Het
Myo3a	G	T	2: 22,245,567	D82Y	possibly damaging	Het
Nsd1	T	A	13: 55,247,641	D1121E	probably benign	Het
Olfr1042	C	T	2: 86,159,456	V305I	probably benign	Het
Olfr1221	G	A	2: 89,112,501	Q4*	probably null	Het
Otoa	T	C	7: 121,118,572	V19A	unknown	Het
Pcdhb20	A	T	18: 37,505,386	T322S	probably benign	Het
Pecam1	T	C	11: 106,695,919	T257A	probably benign	Het
Pi16	G	T	17: 29,319,098		probably benign	Het
Pik3c2g	C	T	6: 139,754,863	T293M		Het
Pkhd1l1	T	A	15: 44,528,163	C1542S	possibly damaging	Het
Prrc2b	G	A	2: 32,229,297	G2172R	probably damaging	Het
Prrt1	A	T	17: 34,629,703		probably null	Het
Ptprb	T	A	10: 116,338,776	N784K	possibly damaging	Het
Rem1	C	A	2: 152,628,149	S18R	probably damaging	Het
Ripk4	G	A	16: 97,747,323		probably null	Het
Scn8a	G	A	15: 101,029,830	V1397I	possibly damaging	Het
Setbp1	T	A	18: 78,856,837	H1205L	probably damaging	Het
Shmt1	T	C	11: 60,801,535	I132V	probably damaging	Het
Slc24a1	T	A	9: 64,928,503	T781S	unknown	Het
Sncaip	C	T	18: 52,907,343	Q870*	probably null	Het
Stab1	A	T	14: 31,160,797	N416K	possibly damaging	Het
Tcea1	A	G	1: 4,867,483	D26G	probably damaging	Het
Tcf20	A	T	15: 82,853,489	S1254T	probably benign	Het
Tead4	T	A	6: 128,228,678	I354F	probably damaging	Het
Tex36	G	A	7: 133,587,418	R142*	probably null	Het
Trav6d-3	T	A	14: 52,725,342	L12Q	probably damaging	Het
Trpc4	A	G	3: 54,194,896	T72A	possibly damaging	Het
Trrap	G	A	5: 144,797,135	A933T	probably benign	Het
Tspoap1	T	A	11: 87,770,489	I589N	probably benign	Het
Ttll5	T	A	12: 85,933,396	V918E	probably benign	Het
Txn2	A	G	15: 77,927,686		probably null	Het
Ucn3	T	G	13: 3,941,365	T96P	probably benign	Het
Usp36	T	C	11: 118,265,154	E764G	possibly damaging	Het
Vmn2r23	A	T	6: 123,704,364	H77L	probably benign	Het
Vmn2r57	T	C	7: 41,400,286	T680A	probably benign	Het
Vwa3a	T	C	7: 120,795,630	I891T	possibly damaging	Het
Zcchc11	T	C	4: 108,527,008	Y1091H	probably damaging	Het
Zhx2	A	G	15: 57,823,643	I803V	probably benign	Het

Other mutations in Olfr918

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00958:Olfr918	APN	9	38673024	missense	probably benign	0.01
IGL01388:Olfr918	APN	9	38673083	nonsense	probably null
IGL01516:Olfr918	APN	9	38672863	missense	probably benign	0.09
IGL02121:Olfr918	APN	9	38673415	missense	probably damaging	0.98
IGL02209:Olfr918	APN	9	38673046	missense	possibly damaging	0.84
IGL02256:Olfr918	APN	9	38673480	start codon destroyed	probably null
IGL02517:Olfr918	APN	9	38672913	missense	probably damaging	1.00
IGL02648:Olfr918	APN	9	38673016	missense	probably benign
IGL02747:Olfr918	APN	9	38673084	missense	probably benign	0.11
IGL02971:Olfr918	APN	9	38673268	missense	probably damaging	0.96
E0370:Olfr918	UTSW	9	38672561	missense	probably damaging	0.99
R0616:Olfr918	UTSW	9	38673480	start codon destroyed	probably null
R2173:Olfr918	UTSW	9	38672944	missense	probably benign	0.03
R2989:Olfr918	UTSW	9	38672535	missense	probably benign
R3430:Olfr918	UTSW	9	38673139	missense	probably damaging	1.00
R3809:Olfr918	UTSW	9	38672863	missense	probably benign	0.09
R4688:Olfr918	UTSW	9	38673363	missense	probably damaging	1.00
R4702:Olfr918	UTSW	9	38673480	start codon destroyed	probably null
R5548:Olfr918	UTSW	9	38673304	missense	probably benign	0.00
R5590:Olfr918	UTSW	9	38672965	missense	probably damaging	1.00
R6082:Olfr918	UTSW	9	38672570	missense	probably damaging	1.00
R6214:Olfr918	UTSW	9	38673214	missense	probably benign	0.13
R6215:Olfr918	UTSW	9	38673214	missense	probably benign	0.13
R6893:Olfr918	UTSW	9	38673059	missense	possibly damaging	0.95
R7624:Olfr918	UTSW	9	38672623	missense	probably benign	0.18
R7862:Olfr918	UTSW	9	38673328	missense	probably benign	0.01
R8116:Olfr918	UTSW	9	38673168	missense	possibly damaging	0.93
R8897:Olfr918	UTSW	9	38672851	missense	probably damaging	1.00
R8899:Olfr918	UTSW	9	38672851	missense	probably damaging	1.00
R9114:Olfr918	UTSW	9	38672596	missense	probably benign
R9293:Olfr918	UTSW	9	38673118	missense	probably damaging	1.00
R9311:Olfr918	UTSW	9	38672629	missense	probably damaging	1.00
R9690:Olfr918	UTSW	9	38673181	nonsense	probably null
R9734:Olfr918	UTSW	9	38672943	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TACAGGTGAGGATTGAGGCC -3'
(R):5'- CCTCAATAAGTTTGCCAGTGG -3'

Sequencing Primer
(F):5'- TTGAGGCCAATCAGGATGATC -3'
(R):5'- GCCAGTGGGTTTTATTTGATCATTC -3'

Posted On

2019-06-26