Mutagenetix > Incidental Mutation

Incidental Mutation 'R7216:Lama3'

561515

Institutional Source

Beutler Lab

Gene Symbol

Lama3

Ensembl Gene

ENSMUSG00000024421

Gene Name

laminin, alpha 3

Synonyms

[a]3B, nicein, 150kDa

MMRRC Submission

045288-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7216 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

12333819-12583013 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 12430000 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Isoleucine at position 527 (F527I)

Ref Sequence

ENSEMBL: ENSMUSP00000089703 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000092070]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000092070
AA Change: F527I

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000089703
Gene: ENSMUSG00000024421
AA Change: F527I

Domain	Start	End	E-Value	Type
signal peptide	1	31	N/A	INTRINSIC
LamNT	38	294	1.46e-153	SMART
EGF_Lam	296	350	1.39e-4	SMART
EGF_Lam	353	420	2.66e-10	SMART
EGF_Lam	423	464	3.51e-10	SMART
EGF_Lam	488	530	1.73e-9	SMART
EGF_Lam	533	576	3.81e-11	SMART
EGF_like	579	625	1.82e-1	SMART
EGF_Lam	628	678	5.15e-8	SMART
EGF_Lam	681	725	3.54e-6	SMART
low complexity region	768	781	N/A	INTRINSIC
EGF_Lam	1263	1306	3.15e-12	SMART
EGF_Lam	1309	1350	6.3e-3	SMART
EGF_Lam	1353	1399	1.49e-13	SMART
EGF_Lam	1402	1450	8.18e-11	SMART
LamB	1509	1638	4.34e-55	SMART
Pfam:Laminin_EGF	1647	1681	7.9e-5	PFAM
EGF_Lam	1684	1728	2.66e-10	SMART
EGF_Lam	1731	1781	7.81e-8	SMART
Pfam:Laminin_I	1836	2102	2.7e-93	PFAM
low complexity region	2185	2200	N/A	INTRINSIC
coiled coil region	2211	2238	N/A	INTRINSIC
LamG	2406	2566	1.67e-2	SMART
LamG	2614	2742	1.72e-17	SMART
LamG	2785	2900	3.96e-17	SMART
LamG	3005	3133	1.12e-34	SMART
LamG	3175	3308	3.41e-30	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]
PHENOTYPE: Mice homozygous for a targeted null mutation develop a lethal blistering phenotype similar to human junctional epidermolysis bullosa, and die 2-3 days after birth from a failure to thrive. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 88 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Akap6	T	A	12: 53,140,457 (GRCm38)	N1551K	probably benign	Het
Amy2a1	T	C	3: 113,530,441 (GRCm38)	D150G	possibly damaging	Het
Atp2b1	T	A	10: 98,986,977 (GRCm38)	C165S	probably benign	Het
Atp2c1	A	T	9: 105,467,731 (GRCm38)	D102E	probably benign	Het
Capn8	C	A	1: 182,598,798 (GRCm38)	R233S	possibly damaging	Het
Cd48	T	C	1: 171,695,822 (GRCm38)	S78P	probably damaging	Het
Clasp1	T	A	1: 118,547,918 (GRCm38)	D944E	probably benign	Het
Clip2	A	G	5: 134,502,917 (GRCm38)	M678T	probably benign	Het
Clmp	T	C	9: 40,760,909 (GRCm38)	Y12H	possibly damaging	Het
Col22a1	A	T	15: 71,973,845 (GRCm38)	V356D	probably damaging	Het
Copg2	A	T	6: 30,885,600 (GRCm38)	D101E	probably damaging	Het
Cpsf6	G	A	10: 117,362,023 (GRCm38)	P229S	unknown	Het
Dgke	T	C	11: 89,050,337 (GRCm38)	D340G	probably benign	Het
Dlg1	TAAA	TAA	16: 31,796,918 (GRCm38)		probably null	Het
Dlg5	G	A	14: 24,136,638 (GRCm38)	Q1865*	probably null	Het
Dnajc2	C	T	5: 21,776,779 (GRCm38)	R123Q	probably damaging	Het
E430018J23Rik	A	G	7: 127,391,523 (GRCm38)	S431P	probably benign	Het
Egln2	T	C	7: 27,159,829 (GRCm38)	D365G	probably damaging	Het
Epha4	T	C	1: 77,444,984 (GRCm38)	D287G	probably damaging	Het
Fat4	A	G	3: 38,891,043 (GRCm38)	T1362A	probably damaging	Het
Flt4	A	G	11: 49,634,681 (GRCm38)	T685A	possibly damaging	Het
Fras1	T	C	5: 96,739,314 (GRCm38)	Y2652H	probably damaging	Het
Gas2	A	G	7: 51,897,257 (GRCm38)	E52G	possibly damaging	Het
Gm11639	A	T	11: 104,880,549 (GRCm38)	K2633N	possibly damaging	Het
Gm13103	A	G	4: 143,851,829 (GRCm38)	I220V	probably damaging	Het
Gm5093	A	T	17: 46,440,088 (GRCm38)	D4E	not run	Het
Gm6370	A	T	5: 146,493,913 (GRCm38)	T303S	probably benign	Het
Gpr161	A	G	1: 165,306,546 (GRCm38)	I126V	probably benign	Het
Gpr63	T	C	4: 25,008,038 (GRCm38)	L254P	probably damaging	Het
H2-M9	C	T	17: 36,640,702 (GRCm38)	V294I	probably benign	Het
Insr	T	A	8: 3,203,034 (GRCm38)	N375I	possibly damaging	Het
Ints1	T	C	5: 139,768,984 (GRCm38)	N600S	possibly damaging	Het
Ints10	A	G	8: 68,822,157 (GRCm38)	N628S	probably damaging	Het
Irf2	A	G	8: 46,793,556 (GRCm38)	T20A	probably benign	Het
Irs1	T	A	1: 82,289,755 (GRCm38)	T247S	probably damaging	Het
Islr	C	T	9: 58,156,967 (GRCm38)	S419N	unknown	Het
Kcna2	T	A	3: 107,104,793 (GRCm38)	I230N	probably damaging	Het
Kif3c	C	A	12: 3,366,126 (GRCm38)	A49E	probably benign	Het
Kifap3	A	T	1: 163,795,989 (GRCm38)	K108N	probably damaging	Het
Layn	A	G	9: 51,077,052 (GRCm38)		probably benign	Het
Lims2	G	T	18: 31,957,262 (GRCm38)	W276L	probably damaging	Het
Lsp1	T	C	7: 142,488,442 (GRCm38)	L133S	probably damaging	Het
Map3k6	T	G	4: 133,246,900 (GRCm38)	V536G	probably damaging	Het
Med7	T	A	11: 46,440,854 (GRCm38)	L92H	probably damaging	Het
Mllt1	A	G	17: 56,927,042 (GRCm38)	V48A	probably damaging	Het
Mpp5	G	A	12: 78,797,232 (GRCm38)	R70H	probably damaging	Het
Neurl4	T	A	11: 69,910,262 (GRCm38)	V1153E	probably damaging	Het
Nkx2-1	A	C	12: 56,534,802 (GRCm38)	C87G	probably damaging	Het
Nsun7	T	C	5: 66,278,657 (GRCm38)	S291P	probably damaging	Het
Olfr148	A	T	9: 39,614,494 (GRCm38)	Q309L	probably benign	Het
Olfr1508	G	A	14: 52,463,488 (GRCm38)	P174S	probably damaging	Het
Olfr406	A	T	11: 74,269,724 (GRCm38)	I112F	probably damaging	Het
Olfr46	A	T	7: 140,610,460 (GRCm38)	N90I	possibly damaging	Het
Olfr829	T	A	9: 18,857,336 (GRCm38)	M237K	probably benign	Het
Pbrm1	A	G	14: 31,045,422 (GRCm38)	Y331C	possibly damaging	Het
Pirb	T	A	7: 3,716,274 (GRCm38)	T539S	probably benign	Het
Plch2	T	G	4: 154,984,228 (GRCm38)	T1314P	probably benign	Het
Polk	T	C	13: 96,508,220 (GRCm38)	S133G	probably benign	Het
Prdm5	A	T	6: 65,927,983 (GRCm38)	K533*	probably null	Het
Prmt1	T	A	7: 44,983,573 (GRCm38)	Q35H	probably benign	Het
Pum3	A	T	19: 27,424,225 (GRCm38)	W142R	probably damaging	Het
Rest	G	A	5: 77,282,608 (GRCm38)	R958H	probably benign	Het
Rictor	C	T	15: 6,769,301 (GRCm38)	T343M	probably damaging	Het
Ripor2	G	A	13: 24,671,903 (GRCm38)	G109R	probably damaging	Het
Sec62	C	T	3: 30,818,829 (GRCm38)	Q354*	probably null	Het
Snx14	T	C	9: 88,381,791 (GRCm38)	Y847C	probably damaging	Het
Soga1	T	C	2: 157,018,370 (GRCm38)	R1650G	possibly damaging	Het
Sox7	A	T	14: 63,947,989 (GRCm38)	D158V	probably benign	Het
Spag8	A	G	4: 43,652,034 (GRCm38)	V350A	possibly damaging	Het
Syngr1	A	T	15: 80,111,733 (GRCm38)	I158F	probably damaging	Het
Tacstd2	C	A	6: 67,534,995 (GRCm38)	V238L	probably benign	Het
Tex15	T	C	8: 33,572,986 (GRCm38)	S815P	possibly damaging	Het
Tmem117	A	T	15: 94,714,912 (GRCm38)	T110S	possibly damaging	Het
Tnrc6a	T	C	7: 123,171,495 (GRCm38)	I836T	probably benign	Het
Trim30b	C	A	7: 104,357,362 (GRCm38)	V96L	probably benign	Het
Tssk3	TGCTATCATGGGG	TG	4: 129,489,313 (GRCm38)		probably null	Het
Tyk2	G	T	9: 21,120,526 (GRCm38)	H418N	probably benign	Het
Uap1	G	T	1: 170,158,903 (GRCm38)	T170K	probably damaging	Het
Vmn1r40	T	C	6: 89,714,624 (GRCm38)	V141A	not run	Het
Vmn1r54	T	A	6: 90,269,665 (GRCm38)	I187K	probably damaging	Het
Vmp1	C	T	11: 86,602,033 (GRCm38)	V317M	probably damaging	Het
Wnt8b	A	G	19: 44,512,072 (GRCm38)	K366R	probably benign	Het
Zfp207	C	T	11: 80,395,178 (GRCm38)	P415L	unknown	Het
Zfp263	A	G	16: 3,744,571 (GRCm38)	E66G	probably damaging	Het
Zfp985	T	A	4: 147,583,456 (GRCm38)	H260Q	probably damaging	Het
Zmiz1	A	C	14: 25,576,207 (GRCm38)	Q46P	probably damaging	Het
Zmiz1	A	G	14: 25,576,209 (GRCm38)	R47G	probably damaging	Het
Zmiz1	GCC	GC	14: 25,576,200 (GRCm38)		probably null	Het

Other mutations in Lama3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00091:Lama3	APN	18	12,580,292 (GRCm38)	missense	probably benign
IGL00272:Lama3	APN	18	12,491,548 (GRCm38)	missense	probably damaging	1.00
IGL00335:Lama3	APN	18	12,449,588 (GRCm38)	splice site	probably benign
IGL00836:Lama3	APN	18	12,472,228 (GRCm38)	missense	probably benign	0.01
IGL01017:Lama3	APN	18	12,441,143 (GRCm38)	critical splice donor site	probably null
IGL01025:Lama3	APN	18	12,481,037 (GRCm38)	missense	probably benign	0.09
IGL01394:Lama3	APN	18	12,531,926 (GRCm38)	missense	probably null	0.39
IGL01545:Lama3	APN	18	12,441,131 (GRCm38)	missense	probably benign	0.01
IGL01685:Lama3	APN	18	12,453,880 (GRCm38)	splice site	probably benign
IGL01863:Lama3	APN	18	12,419,936 (GRCm38)	splice site	probably benign
IGL01869:Lama3	APN	18	12,524,763 (GRCm38)	missense	possibly damaging	0.94
IGL01894:Lama3	APN	18	12,572,064 (GRCm38)	missense	probably benign	0.09
IGL02027:Lama3	APN	18	12,516,513 (GRCm38)	missense	probably damaging	1.00
IGL02106:Lama3	APN	18	12,468,314 (GRCm38)	missense	probably damaging	0.98
IGL02307:Lama3	APN	18	12,581,783 (GRCm38)	missense	probably benign	0.09
IGL02342:Lama3	APN	18	12,491,476 (GRCm38)	missense	probably damaging	1.00
IGL02377:Lama3	APN	18	12,556,750 (GRCm38)	missense	possibly damaging	0.49
IGL02401:Lama3	APN	18	12,557,727 (GRCm38)	missense	probably benign	0.02
IGL02517:Lama3	APN	18	12,537,858 (GRCm38)	critical splice donor site	probably null
IGL02644:Lama3	APN	18	12,525,853 (GRCm38)	missense	probably benign	0.12
IGL02733:Lama3	APN	18	12,578,127 (GRCm38)	missense	probably damaging	0.99
IGL02932:Lama3	APN	18	12,528,801 (GRCm38)	missense	probably damaging	1.00
IGL03006:Lama3	APN	18	12,468,368 (GRCm38)	splice site	probably benign
IGL03038:Lama3	APN	18	12,419,250 (GRCm38)	missense	probably damaging	0.99
IGL03064:Lama3	APN	18	12,439,349 (GRCm38)	missense	possibly damaging	0.72
IGL03146:Lama3	APN	18	12,527,624 (GRCm38)	missense	possibly damaging	0.66
IGL03233:Lama3	APN	18	12,481,038 (GRCm38)	missense	probably damaging	1.00
IGL03255:Lama3	APN	18	12,539,703 (GRCm38)	missense	probably damaging	1.00
IGL03369:Lama3	APN	18	12,553,283 (GRCm38)	missense	probably benign	0.05
IGL03412:Lama3	APN	18	12,419,182 (GRCm38)	missense	probably damaging	0.99
IGL02980:Lama3	UTSW	18	12,553,231 (GRCm38)	missense	probably benign	0.01
IGL03014:Lama3	UTSW	18	12,539,967 (GRCm38)	missense	possibly damaging	0.95
R0007:Lama3	UTSW	18	12,497,881 (GRCm38)	splice site	probably benign
R0007:Lama3	UTSW	18	12,497,881 (GRCm38)	splice site	probably benign
R0050:Lama3	UTSW	18	12,404,103 (GRCm38)	missense	probably damaging	1.00
R0050:Lama3	UTSW	18	12,404,103 (GRCm38)	missense	probably damaging	1.00
R0063:Lama3	UTSW	18	12,528,705 (GRCm38)	splice site	probably benign
R0063:Lama3	UTSW	18	12,528,705 (GRCm38)	splice site	probably benign
R0106:Lama3	UTSW	18	12,403,982 (GRCm38)	missense	probably damaging	0.96
R0148:Lama3	UTSW	18	12,448,272 (GRCm38)	missense	probably damaging	1.00
R0165:Lama3	UTSW	18	12,524,810 (GRCm38)	missense	probably damaging	0.99
R0240:Lama3	UTSW	18	12,539,823 (GRCm38)	splice site	probably null
R0240:Lama3	UTSW	18	12,539,823 (GRCm38)	splice site	probably null
R0316:Lama3	UTSW	18	12,519,877 (GRCm38)	missense	probably benign	0.09
R0325:Lama3	UTSW	18	12,482,126 (GRCm38)	missense	probably damaging	1.00
R0365:Lama3	UTSW	18	12,507,007 (GRCm38)	missense	probably damaging	0.96
R0390:Lama3	UTSW	18	12,407,563 (GRCm38)	missense	probably benign	0.10
R0408:Lama3	UTSW	18	12,456,837 (GRCm38)	missense	probably benign
R0449:Lama3	UTSW	18	12,500,512 (GRCm38)	splice site	probably null
R0453:Lama3	UTSW	18	12,465,478 (GRCm38)	missense	possibly damaging	0.63
R0480:Lama3	UTSW	18	12,450,424 (GRCm38)	missense	possibly damaging	0.81
R0536:Lama3	UTSW	18	12,525,894 (GRCm38)	missense	probably damaging	1.00
R0545:Lama3	UTSW	18	12,561,701 (GRCm38)	missense	possibly damaging	0.90
R0567:Lama3	UTSW	18	12,549,252 (GRCm38)	missense	probably benign
R0605:Lama3	UTSW	18	12,506,949 (GRCm38)	missense	probably benign	0.02
R0617:Lama3	UTSW	18	12,419,258 (GRCm38)	critical splice donor site	probably null
R0629:Lama3	UTSW	18	12,419,245 (GRCm38)	missense	possibly damaging	0.79
R0671:Lama3	UTSW	18	12,477,590 (GRCm38)	missense	possibly damaging	0.80
R0730:Lama3	UTSW	18	12,456,850 (GRCm38)	splice site	probably benign
R1216:Lama3	UTSW	18	12,421,134 (GRCm38)	splice site	probably benign
R1356:Lama3	UTSW	18	12,500,577 (GRCm38)	unclassified	probably benign
R1386:Lama3	UTSW	18	12,477,370 (GRCm38)	missense	probably benign	0.04
R1424:Lama3	UTSW	18	12,519,991 (GRCm38)	missense	probably benign	0.13
R1426:Lama3	UTSW	18	12,481,098 (GRCm38)	critical splice donor site	probably null
R1437:Lama3	UTSW	18	12,549,227 (GRCm38)	missense	possibly damaging	0.46
R1468:Lama3	UTSW	18	12,441,107 (GRCm38)	missense	probably benign	0.00
R1468:Lama3	UTSW	18	12,441,107 (GRCm38)	missense	probably benign	0.00
R1472:Lama3	UTSW	18	12,482,045 (GRCm38)	missense	probably benign	0.23
R1557:Lama3	UTSW	18	12,513,731 (GRCm38)	splice site	probably benign
R1571:Lama3	UTSW	18	12,539,717 (GRCm38)	missense	probably damaging	0.98
R1599:Lama3	UTSW	18	12,450,400 (GRCm38)	nonsense	probably null
R1631:Lama3	UTSW	18	12,407,494 (GRCm38)	missense	probably damaging	1.00
R1647:Lama3	UTSW	18	12,532,199 (GRCm38)	missense	possibly damaging	0.90
R1648:Lama3	UTSW	18	12,532,199 (GRCm38)	missense	possibly damaging	0.90
R1719:Lama3	UTSW	18	12,479,872 (GRCm38)	critical splice donor site	probably null
R1757:Lama3	UTSW	18	12,465,499 (GRCm38)	missense	probably benign	0.10
R1766:Lama3	UTSW	18	12,402,062 (GRCm38)	missense	probably damaging	1.00
R1853:Lama3	UTSW	18	12,513,705 (GRCm38)	missense	possibly damaging	0.75
R1856:Lama3	UTSW	18	12,537,781 (GRCm38)	nonsense	probably null
R1909:Lama3	UTSW	18	12,581,798 (GRCm38)	missense	probably benign	0.19
R1913:Lama3	UTSW	18	12,495,279 (GRCm38)	missense	probably benign	0.15
R1975:Lama3	UTSW	18	12,453,863 (GRCm38)	missense	probably damaging	1.00
R2014:Lama3	UTSW	18	12,524,721 (GRCm38)	splice site	probably benign
R2059:Lama3	UTSW	18	12,528,333 (GRCm38)	missense	probably damaging	0.98
R2060:Lama3	UTSW	18	12,528,726 (GRCm38)	missense	probably benign	0.30
R2086:Lama3	UTSW	18	12,524,830 (GRCm38)	missense	probably benign	0.39
R2115:Lama3	UTSW	18	12,402,849 (GRCm38)	missense	possibly damaging	0.94
R2291:Lama3	UTSW	18	12,525,079 (GRCm38)	missense	probably damaging	0.98
R2860:Lama3	UTSW	18	12,453,750 (GRCm38)	missense	probably damaging	1.00
R2861:Lama3	UTSW	18	12,453,750 (GRCm38)	missense	probably damaging	1.00
R2862:Lama3	UTSW	18	12,453,750 (GRCm38)	missense	probably damaging	1.00
R3410:Lama3	UTSW	18	12,413,858 (GRCm38)	critical splice donor site	probably null
R3614:Lama3	UTSW	18	12,448,288 (GRCm38)	missense	probably benign	0.03
R3696:Lama3	UTSW	18	12,439,475 (GRCm38)	splice site	probably benign
R3752:Lama3	UTSW	18	12,507,029 (GRCm38)	missense	probably damaging	1.00
R3967:Lama3	UTSW	18	12,580,341 (GRCm38)	missense	probably damaging	1.00
R3968:Lama3	UTSW	18	12,580,341 (GRCm38)	missense	probably damaging	1.00
R3969:Lama3	UTSW	18	12,580,341 (GRCm38)	missense	probably damaging	1.00
R3970:Lama3	UTSW	18	12,580,341 (GRCm38)	missense	probably damaging	1.00
R4088:Lama3	UTSW	18	12,504,308 (GRCm38)	nonsense	probably null
R4118:Lama3	UTSW	18	12,450,431 (GRCm38)	missense	probably benign	0.01
R4222:Lama3	UTSW	18	12,450,403 (GRCm38)	missense	probably damaging	1.00
R4223:Lama3	UTSW	18	12,450,403 (GRCm38)	missense	probably damaging	1.00
R4224:Lama3	UTSW	18	12,450,403 (GRCm38)	missense	probably damaging	1.00
R4225:Lama3	UTSW	18	12,450,403 (GRCm38)	missense	probably damaging	1.00
R4367:Lama3	UTSW	18	12,513,690 (GRCm38)	missense	probably damaging	1.00
R4404:Lama3	UTSW	18	12,582,531 (GRCm38)	missense	probably benign	0.01
R4424:Lama3	UTSW	18	12,519,872 (GRCm38)	nonsense	probably null
R4483:Lama3	UTSW	18	12,549,253 (GRCm38)	missense	probably benign	0.32
R4484:Lama3	UTSW	18	12,481,088 (GRCm38)	missense	probably benign
R4516:Lama3	UTSW	18	12,495,358 (GRCm38)	missense	probably damaging	1.00
R4556:Lama3	UTSW	18	12,479,759 (GRCm38)	missense	possibly damaging	0.63
R4616:Lama3	UTSW	18	12,504,397 (GRCm38)	critical splice donor site	probably null
R4702:Lama3	UTSW	18	12,578,029 (GRCm38)	nonsense	probably null
R4704:Lama3	UTSW	18	12,553,223 (GRCm38)	missense	probably benign	0.08
R4750:Lama3	UTSW	18	12,504,359 (GRCm38)	missense	probably benign	0.25
R4753:Lama3	UTSW	18	12,482,084 (GRCm38)	missense	probably damaging	1.00
R4767:Lama3	UTSW	18	12,500,563 (GRCm38)	missense	probably benign	0.32
R4777:Lama3	UTSW	18	12,413,771 (GRCm38)	missense	probably damaging	1.00
R4782:Lama3	UTSW	18	12,411,570 (GRCm38)	nonsense	probably null
R4784:Lama3	UTSW	18	12,449,544 (GRCm38)	missense	probably benign	0.20
R4816:Lama3	UTSW	18	12,477,604 (GRCm38)	missense	possibly damaging	0.93
R4833:Lama3	UTSW	18	12,441,131 (GRCm38)	missense	probably benign	0.01
R4854:Lama3	UTSW	18	12,411,542 (GRCm38)	missense	probably benign	0.00
R4863:Lama3	UTSW	18	12,498,678 (GRCm38)	intron	probably benign
R4863:Lama3	UTSW	18	12,539,793 (GRCm38)	missense	probably damaging	0.99
R4953:Lama3	UTSW	18	12,448,305 (GRCm38)	missense	probably damaging	1.00
R4974:Lama3	UTSW	18	12,552,826 (GRCm38)	missense	probably damaging	0.98
R4996:Lama3	UTSW	18	12,518,743 (GRCm38)	missense	probably benign	0.24
R5049:Lama3	UTSW	18	12,582,611 (GRCm38)	missense	probably benign	0.19
R5057:Lama3	UTSW	18	12,531,948 (GRCm38)	missense	probably null	0.82
R5090:Lama3	UTSW	18	12,542,402 (GRCm38)	missense	possibly damaging	0.94
R5122:Lama3	UTSW	18	12,539,766 (GRCm38)	missense	possibly damaging	0.53
R5215:Lama3	UTSW	18	12,577,900 (GRCm38)	missense	probably damaging	1.00
R5245:Lama3	UTSW	18	12,419,893 (GRCm38)	missense	probably damaging	1.00
R5259:Lama3	UTSW	18	12,465,508 (GRCm38)	missense	probably damaging	1.00
R5320:Lama3	UTSW	18	12,552,855 (GRCm38)	missense	probably damaging	0.99
R5377:Lama3	UTSW	18	12,453,746 (GRCm38)	missense	probably damaging	0.99
R5432:Lama3	UTSW	18	12,572,066 (GRCm38)	missense	probably damaging	1.00
R5500:Lama3	UTSW	18	12,456,764 (GRCm38)	missense	possibly damaging	0.93
R5534:Lama3	UTSW	18	12,553,210 (GRCm38)	missense	probably benign	0.00
R5589:Lama3	UTSW	18	12,472,220 (GRCm38)	missense	possibly damaging	0.46
R5604:Lama3	UTSW	18	12,439,348 (GRCm38)	missense	probably benign
R5617:Lama3	UTSW	18	12,498,936 (GRCm38)	intron	probably benign
R5709:Lama3	UTSW	18	12,539,799 (GRCm38)	missense	probably damaging	1.00
R5965:Lama3	UTSW	18	12,429,887 (GRCm38)	missense	possibly damaging	0.67
R6042:Lama3	UTSW	18	12,574,254 (GRCm38)	missense	probably damaging	1.00
R6065:Lama3	UTSW	18	12,469,928 (GRCm38)	missense	possibly damaging	0.53
R6085:Lama3	UTSW	18	12,482,099 (GRCm38)	missense	probably benign	0.01
R6212:Lama3	UTSW	18	12,513,645 (GRCm38)	missense	probably damaging	1.00
R6268:Lama3	UTSW	18	12,524,737 (GRCm38)	missense	probably damaging	0.98
R6276:Lama3	UTSW	18	12,506,949 (GRCm38)	missense	probably benign	0.02
R6366:Lama3	UTSW	18	12,482,137 (GRCm38)	missense	probably damaging	1.00
R6393:Lama3	UTSW	18	12,479,756 (GRCm38)	missense	probably benign	0.44
R6493:Lama3	UTSW	18	12,482,148 (GRCm38)	critical splice donor site	probably null
R6505:Lama3	UTSW	18	12,495,348 (GRCm38)	missense	probably benign	0.02
R6563:Lama3	UTSW	18	12,537,766 (GRCm38)	missense	probably damaging	1.00
R6582:Lama3	UTSW	18	12,577,840 (GRCm38)	missense	probably damaging	1.00
R6585:Lama3	UTSW	18	12,419,257 (GRCm38)	critical splice donor site	probably null
R6609:Lama3	UTSW	18	12,513,678 (GRCm38)	missense	probably damaging	0.99
R6656:Lama3	UTSW	18	12,549,226 (GRCm38)	missense	possibly damaging	0.66
R6833:Lama3	UTSW	18	12,491,548 (GRCm38)	missense	probably damaging	1.00
R6834:Lama3	UTSW	18	12,491,548 (GRCm38)	missense	probably damaging	1.00
R7019:Lama3	UTSW	18	12,528,418 (GRCm38)	missense	probably damaging	0.97
R7026:Lama3	UTSW	18	12,516,548 (GRCm38)	missense	probably damaging	0.98
R7088:Lama3	UTSW	18	12,582,545 (GRCm38)	missense	possibly damaging	0.90
R7100:Lama3	UTSW	18	12,582,644 (GRCm38)	missense	possibly damaging	0.80
R7102:Lama3	UTSW	18	12,552,813 (GRCm38)	missense	possibly damaging	0.66
R7103:Lama3	UTSW	18	12,531,879 (GRCm38)	missense	probably benign	0.00
R7121:Lama3	UTSW	18	12,462,782 (GRCm38)	missense	probably benign	0.06
R7133:Lama3	UTSW	18	12,539,786 (GRCm38)	missense	probably benign	0.05
R7150:Lama3	UTSW	18	12,468,289 (GRCm38)	missense	probably damaging	1.00
R7158:Lama3	UTSW	18	12,456,812 (GRCm38)	missense	probably benign	0.20
R7170:Lama3	UTSW	18	12,404,076 (GRCm38)	missense	probably benign	0.26
R7223:Lama3	UTSW	18	12,582,608 (GRCm38)	missense	possibly damaging	0.53
R7243:Lama3	UTSW	18	12,419,845 (GRCm38)	missense	probably damaging	1.00
R7282:Lama3	UTSW	18	12,439,392 (GRCm38)	missense	probably damaging	0.99
R7337:Lama3	UTSW	18	12,507,040 (GRCm38)	splice site	probably null
R7442:Lama3	UTSW	18	12,472,181 (GRCm38)	critical splice acceptor site	probably null
R7487:Lama3	UTSW	18	12,419,237 (GRCm38)	missense	probably benign
R7604:Lama3	UTSW	18	12,500,493 (GRCm38)	missense	possibly damaging	0.93
R7609:Lama3	UTSW	18	12,531,834 (GRCm38)	critical splice acceptor site	probably null
R7650:Lama3	UTSW	18	12,537,838 (GRCm38)	missense	probably benign	0.01
R7894:Lama3	UTSW	18	12,462,807 (GRCm38)	missense	probably benign	0.07
R7975:Lama3	UTSW	18	12,537,739 (GRCm38)	missense	probably damaging	1.00
R8099:Lama3	UTSW	18	12,534,063 (GRCm38)	missense	probably damaging	0.97
R8168:Lama3	UTSW	18	12,506,942 (GRCm38)	missense	probably null
R8219:Lama3	UTSW	18	12,439,360 (GRCm38)	missense	probably benign	0.07
R8227:Lama3	UTSW	18	12,407,551 (GRCm38)	missense	probably benign
R8229:Lama3	UTSW	18	12,407,551 (GRCm38)	missense	probably benign
R8298:Lama3	UTSW	18	12,525,853 (GRCm38)	missense	probably benign	0.12
R8351:Lama3	UTSW	18	12,540,613 (GRCm38)	missense	probably damaging	1.00
R8364:Lama3	UTSW	18	12,528,347 (GRCm38)	missense	probably damaging	0.99
R8463:Lama3	UTSW	18	12,449,839 (GRCm38)	missense	probably damaging	0.96
R8515:Lama3	UTSW	18	12,411,631 (GRCm38)	missense	probably null	0.01
R8784:Lama3	UTSW	18	12,421,155 (GRCm38)	missense	probably benign
R8799:Lama3	UTSW	18	12,490,943 (GRCm38)	missense	probably damaging	0.96
R8874:Lama3	UTSW	18	12,449,586 (GRCm38)	critical splice donor site	probably null
R8938:Lama3	UTSW	18	12,556,705 (GRCm38)	missense	probably damaging	1.00
R8967:Lama3	UTSW	18	12,532,039 (GRCm38)	missense	possibly damaging	0.46
R9039:Lama3	UTSW	18	12,481,063 (GRCm38)	nonsense	probably null
R9126:Lama3	UTSW	18	12,450,470 (GRCm38)	missense	probably damaging	1.00
R9200:Lama3	UTSW	18	12,472,240 (GRCm38)	missense	probably benign	0.00
R9203:Lama3	UTSW	18	12,462,812 (GRCm38)	missense	probably benign	0.04
R9246:Lama3	UTSW	18	12,577,902 (GRCm38)	missense	probably damaging	0.99
R9284:Lama3	UTSW	18	12,450,484 (GRCm38)	nonsense	probably null
R9553:Lama3	UTSW	18	12,429,962 (GRCm38)	missense	probably damaging	1.00
R9716:Lama3	UTSW	18	12,450,403 (GRCm38)	missense	probably damaging	1.00
R9734:Lama3	UTSW	18	12,549,263 (GRCm38)	missense	possibly damaging	0.94
X0019:Lama3	UTSW	18	12,582,574 (GRCm38)	missense	possibly damaging	0.94
Z1177:Lama3	UTSW	18	12,429,879 (GRCm38)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- TGGGAGAAGCATGAAGTCTATTTGG -3'
(R):5'- ACGCACAGTCCAGGTACTTG -3'

Sequencing Primer
(F):5'- CATGAAGTCTATTTGGGCACAG -3'
(R):5'- GTCCAGGTACTTGACTACCATAGAG -3'

Posted On

2019-06-26