Mutagenetix > Incidental Mutation

Incidental Mutation 'R7218:Wnk1'

561585

Institutional Source

Beutler Lab

Gene Symbol

Wnk1

Ensembl Gene

ENSMUSG00000045962

Gene Name

WNK lysine deficient protein kinase 1

Synonyms

6430573H23Rik, Hsn2, EG406236, Prkwnk1

MMRRC Submission

Accession Numbers

Is this an essential gene?

Essential (E-score: 1.000) question?

Stock #

R7218 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

119923969-120038672 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to C at 120002273 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 284 (Y284*)

Ref Sequence

ENSEMBL: ENSMUSP00000063001 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000060043] [ENSMUST00000088644] [ENSMUST00000088646] [ENSMUST00000177761] [ENSMUST00000203030]

AlphaFold

P83741

Predicted Effect

probably null
Transcript: ENSMUST00000060043
AA Change: Y284*

SMART Domains

Protein: ENSMUSP00000063001
Gene: ENSMUSG00000045962
AA Change: Y284*

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.5e-44	PFAM
Pfam:Pkinase	221	479	4.4e-58	PFAM
Pfam:OSR1_C	500	537	2e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	865	876	N/A	INTRINSIC
low complexity region	1018	1028	N/A	INTRINSIC
low complexity region	1042	1058	N/A	INTRINSIC
internal_repeat_1	1136	1178	2.15e-5	PROSPERO
low complexity region	1289	1305	N/A	INTRINSIC
low complexity region	1345	1357	N/A	INTRINSIC
low complexity region	1379	1396	N/A	INTRINSIC
low complexity region	1398	1428	N/A	INTRINSIC
low complexity region	1429	1454	N/A	INTRINSIC
low complexity region	1496	1515	N/A	INTRINSIC
low complexity region	1542	1556	N/A	INTRINSIC
low complexity region	1586	1609	N/A	INTRINSIC
low complexity region	1670	1691	N/A	INTRINSIC
low complexity region	1698	1712	N/A	INTRINSIC
low complexity region	1738	1764	N/A	INTRINSIC
low complexity region	1882	1900	N/A	INTRINSIC
coiled coil region	2065	2092	N/A	INTRINSIC
low complexity region	2103	2114	N/A	INTRINSIC
low complexity region	2116	2140	N/A	INTRINSIC
low complexity region	2208	2232	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000088644
AA Change: Y284*

SMART Domains

Protein: ENSMUSP00000086017
Gene: ENSMUSG00000045962
AA Change: Y284*

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	5.5e-44	PFAM
Pfam:Pkinase	221	479	4.3e-56	PFAM
Pfam:OSR1_C	500	537	1.9e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	742	753	N/A	INTRINSIC
low complexity region	919	935	N/A	INTRINSIC
low complexity region	1050	1063	N/A	INTRINSIC
low complexity region	1067	1076	N/A	INTRINSIC
low complexity region	1146	1158	N/A	INTRINSIC
low complexity region	1276	1286	N/A	INTRINSIC
low complexity region	1300	1316	N/A	INTRINSIC
internal_repeat_1	1394	1436	2.19e-5	PROSPERO
low complexity region	1547	1563	N/A	INTRINSIC
low complexity region	1603	1615	N/A	INTRINSIC
low complexity region	1637	1654	N/A	INTRINSIC
low complexity region	1656	1686	N/A	INTRINSIC
low complexity region	1687	1712	N/A	INTRINSIC
low complexity region	1754	1773	N/A	INTRINSIC
low complexity region	1800	1814	N/A	INTRINSIC
low complexity region	1844	1867	N/A	INTRINSIC
low complexity region	1928	1949	N/A	INTRINSIC
low complexity region	1956	1970	N/A	INTRINSIC
low complexity region	1996	2022	N/A	INTRINSIC
low complexity region	2140	2158	N/A	INTRINSIC
coiled coil region	2323	2350	N/A	INTRINSIC
low complexity region	2361	2372	N/A	INTRINSIC
low complexity region	2374	2398	N/A	INTRINSIC
low complexity region	2466	2490	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000088646
AA Change: Y284*

SMART Domains

Protein: ENSMUSP00000086019
Gene: ENSMUSG00000045962
AA Change: Y284*

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.3e-44	PFAM
Pfam:Pkinase	221	479	4e-58	PFAM
Pfam:OSR1_C	500	537	1.8e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	864	874	N/A	INTRINSIC
low complexity region	888	904	N/A	INTRINSIC
internal_repeat_1	982	1024	7.56e-6	PROSPERO
low complexity region	1135	1151	N/A	INTRINSIC
low complexity region	1191	1203	N/A	INTRINSIC
low complexity region	1225	1242	N/A	INTRINSIC
low complexity region	1244	1274	N/A	INTRINSIC
low complexity region	1275	1300	N/A	INTRINSIC
low complexity region	1342	1361	N/A	INTRINSIC
low complexity region	1388	1402	N/A	INTRINSIC
low complexity region	1432	1455	N/A	INTRINSIC
low complexity region	1516	1537	N/A	INTRINSIC
low complexity region	1544	1558	N/A	INTRINSIC
low complexity region	1584	1610	N/A	INTRINSIC
low complexity region	1700	1718	N/A	INTRINSIC
coiled coil region	1883	1910	N/A	INTRINSIC
low complexity region	1921	1932	N/A	INTRINSIC
low complexity region	1934	1958	N/A	INTRINSIC
low complexity region	2026	2050	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000177761
AA Change: Y284*

SMART Domains

Protein: ENSMUSP00000136777
Gene: ENSMUSG00000045962
AA Change: Y284*

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.7e-44	PFAM
Pfam:Pkinase	221	479	5.1e-58	PFAM
Pfam:OSR1_C	500	537	2.2e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	710	722	N/A	INTRINSIC
low complexity region	828	839	N/A	INTRINSIC
low complexity region	1005	1021	N/A	INTRINSIC
low complexity region	1136	1149	N/A	INTRINSIC
low complexity region	1153	1162	N/A	INTRINSIC
low complexity region	1232	1244	N/A	INTRINSIC
low complexity region	1291	1307	N/A	INTRINSIC
internal_repeat_1	1385	1427	1.91e-5	PROSPERO
low complexity region	1538	1554	N/A	INTRINSIC
low complexity region	1594	1606	N/A	INTRINSIC
low complexity region	1628	1645	N/A	INTRINSIC
low complexity region	1647	1677	N/A	INTRINSIC
low complexity region	1678	1703	N/A	INTRINSIC
low complexity region	1745	1764	N/A	INTRINSIC
low complexity region	1791	1805	N/A	INTRINSIC
low complexity region	1835	1858	N/A	INTRINSIC
low complexity region	1919	1940	N/A	INTRINSIC
low complexity region	1947	1961	N/A	INTRINSIC
low complexity region	1987	2013	N/A	INTRINSIC
low complexity region	2131	2149	N/A	INTRINSIC
coiled coil region	2314	2341	N/A	INTRINSIC
low complexity region	2352	2363	N/A	INTRINSIC
low complexity region	2365	2389	N/A	INTRINSIC
low complexity region	2457	2481	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000203030
AA Change: Y284*

SMART Domains

Protein: ENSMUSP00000145304
Gene: ENSMUSG00000045962
AA Change: Y284*

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	4.1e-44	PFAM
Pfam:Pkinase	221	479	3.2e-56	PFAM
Pfam:OSR1_C	500	537	1.5e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	793	809	N/A	INTRINSIC
internal_repeat_1	887	929	5.8e-6	PROSPERO
low complexity region	1040	1056	N/A	INTRINSIC
low complexity region	1096	1108	N/A	INTRINSIC
low complexity region	1130	1147	N/A	INTRINSIC
low complexity region	1149	1179	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1247	1266	N/A	INTRINSIC
low complexity region	1293	1307	N/A	INTRINSIC
low complexity region	1337	1360	N/A	INTRINSIC
low complexity region	1421	1442	N/A	INTRINSIC
low complexity region	1449	1463	N/A	INTRINSIC
low complexity region	1489	1515	N/A	INTRINSIC
low complexity region	1633	1651	N/A	INTRINSIC
coiled coil region	1816	1843	N/A	INTRINSIC
low complexity region	1854	1865	N/A	INTRINSIC
low complexity region	1867	1891	N/A	INTRINSIC
low complexity region	1959	1983	N/A	INTRINSIC

Meta Mutation Damage Score

0.9711

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (69/69)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.[provided by RefSeq, May 2010]
PHENOTYPE: Homozygous mutant mice die before birth, whereas heterozygotes survive and exhibit decreased blood pressure. Mice homozygous for an allele that does not produce the kidney isoform exhibit a slight increase in systemic arterial diastolic blood pressure and reduced sensitivity to amiloride. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1600014C23Rik	T	C	17: 45,733,051	T94A	unknown	Het
1700025G04Rik	T	C	1: 151,915,508	R101G	probably damaging	Het
6430548M08Rik	T	A	8: 120,145,583	S83R	probably damaging	Het
9130019O22Rik	A	T	7: 127,384,680	S417T	probably benign	Het
A430089I19Rik	C	A	5: 94,303,254	V338F	probably benign	Het
Actn2	A	G	13: 12,278,913	S574P	probably benign	Het
Ank	A	T	15: 27,544,321	Y56F	probably damaging	Het
Ano7	A	G	1: 93,380,469	D74G	probably benign	Het
Apaf1	T	A	10: 91,037,002	T738S	probably damaging	Het
Apcs	A	T	1: 172,894,664	D38E	possibly damaging	Het
Arntl	A	T	7: 113,287,183	H149L	probably damaging	Het
Asap1	G	A	15: 64,130,250	T404M	probably damaging	Het
Atp8a1	T	A	5: 67,702,981	D717V		Het
Baiap2l1	A	G	5: 144,275,877	S443P	probably benign	Het
Brix1	T	C	15: 10,483,292		probably null	Het
C1qtnf6	T	C	15: 78,527,374	E34G	probably benign	Het
Chil3	A	C	3: 106,160,537		probably null	Het
Chmp4c	T	A	3: 10,367,138	L36Q	probably damaging	Het
Chrna2	T	A	14: 66,143,871		probably null	Het
Clec1a	C	T	6: 129,436,955	C57Y	probably damaging	Het
Clec7a	G	T	6: 129,468,922	T95K	probably damaging	Het
Csf1r	C	A	18: 61,130,324	S926R	probably damaging	Het
Dnajc9	A	G	14: 20,388,439	I61T	probably benign	Het
Extl1	T	G	4: 134,359,769	S493R	probably benign	Het
Fance	A	G	17: 28,326,174	D143G	probably benign	Het
Fcho2	C	T	13: 98,753,613		probably null	Het
Filip1	T	C	9: 79,818,074	S1088G	probably benign	Het
Gm10696	A	T	3: 94,175,549	H318Q	possibly damaging	Het
Gnat1	A	C	9: 107,675,985	M319R	possibly damaging	Het
Gpr132	A	T	12: 112,852,429	V259E	probably damaging	Het
Gprc5d	T	A	6: 135,116,454	M152L	probably benign	Het
Hif3a	C	T	7: 17,050,588	R244H	probably damaging	Het
Hivep3	T	C	4: 120,095,452	S322P	possibly damaging	Het
Il33	T	A	19: 29,958,925	F229I	probably damaging	Het
Il4ra	A	G	7: 125,575,778	D386G	probably benign	Het
Ino80	G	T	2: 119,458,127	H33N	probably benign	Het
Ip6k1	A	G	9: 108,045,582	D228G	unknown	Het
Mamdc2	C	T	19: 23,447,610	A40T	probably benign	Het
Meis3	T	C	7: 16,184,701	V357A	probably benign	Het
Mycbp2	T	C	14: 103,133,846	T4199A	probably benign	Het
Myo7b	A	T	18: 31,981,001	M1099K	probably benign	Het
Mzb1	A	T	18: 35,647,922	H104Q	probably benign	Het
Nfatc2	A	G	2: 168,571,264	L167P	probably benign	Het
Numa1	A	T	7: 102,000,910	S1283C	probably benign	Het
Nup98	T	G	7: 102,191,900		probably null	Het
Olfr1342	T	C	4: 118,690,018	I145V	probably benign	Het
Olfr476	T	C	7: 107,967,667	L90P	probably benign	Het
Pkhd1l1	A	G	15: 44,522,695	T1243A	possibly damaging	Het
Ptpro	C	T	6: 137,454,598	R1152W	probably damaging	Het
Ptprt	T	C	2: 161,547,364	T1270A	probably damaging	Het
Pwwp2b	A	C	7: 139,256,133	T497P	probably damaging	Het
Rab44	T	A	17: 29,139,444	V202E		Het
Rbfox1	C	T	16: 7,294,083	T191I	probably damaging	Het
Rufy4	A	G	1: 74,133,015	K299R	probably damaging	Het
Snip1	T	A	4: 125,072,919	S381T	probably damaging	Het
Spen	T	C	4: 141,472,650	I2889V	possibly damaging	Het
St3gal3	T	A	4: 117,957,442	D218V		Het
Sun1	A	G	5: 139,226,687	T70A	unknown	Het
Tbc1d23	C	T	16: 57,170,382	V678M	probably damaging	Het
Tdh	T	A	14: 63,495,757	Y195F	probably damaging	Het
Tescl	C	T	7: 24,333,861	R13H	possibly damaging	Het
Tfap2c	T	A	2: 172,557,357	M508K	probably benign	Het
Trappc4	A	G	9: 44,405,290	M136T	probably benign	Het
Tyk2	A	G	9: 21,105,054	C1207R	probably damaging	Het
Ugt2b36	T	C	5: 87,081,539	Y355C	probably damaging	Het
Vmn1r119	T	A	7: 21,011,647	H270L	probably benign	Het
Vmn2r20	G	A	6: 123,386,115	P570L	probably damaging	Het
Yars2	C	T	16: 16,303,318	A112V	probably damaging	Het
Zer1	T	C	2: 30,105,012	N470S	probably damaging	Het
Zfp879	A	C	11: 50,832,681	V516G	possibly damaging	Het

Other mutations in Wnk1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00901:Wnk1	APN	6	119960708	missense	probably damaging	1.00
IGL01364:Wnk1	APN	6	119937411	missense	probably damaging	1.00
IGL01715:Wnk1	APN	6	119948397	missense	probably damaging	1.00
IGL01951:Wnk1	APN	6	119963485	missense	probably damaging	0.99
IGL02043:Wnk1	APN	6	119949078	unclassified	probably benign
IGL02268:Wnk1	APN	6	119937373	nonsense	probably null
IGL02348:Wnk1	APN	6	119963328	missense	probably damaging	0.98
IGL02425:Wnk1	APN	6	119963454	missense	probably damaging	1.00
IGL02850:Wnk1	APN	6	119937862	missense	probably benign	0.26
IGL03160:Wnk1	APN	6	119926633	missense	probably damaging	1.00
IGL03387:Wnk1	APN	6	119954187	missense	possibly damaging	0.76
IGL03405:Wnk1	APN	6	119953895	missense	probably benign	0.41
Actor	UTSW	6	119970978	missense	probably damaging	1.00
Bad	UTSW	6	119965678	intron	probably benign
Blink	UTSW	6	120002273	nonsense	probably null
Knock	UTSW	6	119943878	splice site	probably null
Narrow	UTSW	6	119992408	missense	probably damaging	1.00
nictitate	UTSW	6	120002280	missense	possibly damaging	0.88
opportunity	UTSW	6	119951068	missense	probably damaging	1.00
path	UTSW	6	120037149	missense	probably damaging	0.99
Stormy	UTSW	6	119992447	missense	probably damaging	1.00
tear	UTSW	6	119963470	missense	probably damaging	0.98
Tic	UTSW	6	119950083	missense	probably damaging	1.00
Unforgiving	UTSW	6	119948282	missense	probably damaging	1.00
Window	UTSW	6	119992453	missense	probably damaging	1.00
Woke	UTSW	6	119962955	missense	probably benign	0.12
IGL03052:Wnk1	UTSW	6	119944799	splice site	probably benign
PIT4480001:Wnk1	UTSW	6	119963367	nonsense	probably null
R0044:Wnk1	UTSW	6	120037149	missense	probably damaging	0.99
R0207:Wnk1	UTSW	6	119952733	missense	probably damaging	1.00
R0333:Wnk1	UTSW	6	119928163	intron	probably benign
R0453:Wnk1	UTSW	6	119963151	missense	probably damaging	0.99
R0457:Wnk1	UTSW	6	119969332	missense	probably damaging	1.00
R0501:Wnk1	UTSW	6	119962803	missense	probably damaging	1.00
R0525:Wnk1	UTSW	6	119926564	missense	probably damaging	1.00
R0526:Wnk1	UTSW	6	119951992	missense	probably damaging	0.99
R0606:Wnk1	UTSW	6	119926683	missense	probably damaging	1.00
R0658:Wnk1	UTSW	6	119948505	missense	probably damaging	0.98
R1148:Wnk1	UTSW	6	119952006	splice site	probably benign
R1188:Wnk1	UTSW	6	119948709	nonsense	probably null
R1245:Wnk1	UTSW	6	119948457	missense	probably benign	0.26
R1449:Wnk1	UTSW	6	119952818	missense	probably damaging	1.00
R1469:Wnk1	UTSW	6	119950684	splice site	probably benign
R1869:Wnk1	UTSW	6	119951089	missense	probably damaging	1.00
R1871:Wnk1	UTSW	6	119951089	missense	probably damaging	1.00
R1928:Wnk1	UTSW	6	119952923	missense	probably damaging	1.00
R1959:Wnk1	UTSW	6	119969247	missense	probably damaging	0.98
R1961:Wnk1	UTSW	6	119969247	missense	probably damaging	0.98
R1964:Wnk1	UTSW	6	119934382	missense	possibly damaging	0.86
R1983:Wnk1	UTSW	6	119937578	missense	probably damaging	1.00
R2062:Wnk1	UTSW	6	119928157	splice site	probably null
R2144:Wnk1	UTSW	6	119948988	unclassified	probably benign
R2186:Wnk1	UTSW	6	119948567	missense	probably benign	0.26
R2281:Wnk1	UTSW	6	119963640	splice site	probably null
R2338:Wnk1	UTSW	6	119969534	missense	probably benign	0.42
R2420:Wnk1	UTSW	6	119936367	critical splice donor site	probably null
R3727:Wnk1	UTSW	6	119992453	missense	probably damaging	1.00
R3773:Wnk1	UTSW	6	120002280	missense	possibly damaging	0.88
R3836:Wnk1	UTSW	6	119950043	missense	probably damaging	1.00
R3837:Wnk1	UTSW	6	119950043	missense	probably damaging	1.00
R3847:Wnk1	UTSW	6	119969354	missense	possibly damaging	0.70
R3903:Wnk1	UTSW	6	119949051	missense	probably damaging	1.00
R4031:Wnk1	UTSW	6	119951068	missense	probably damaging	1.00
R4095:Wnk1	UTSW	6	119948126	missense	probably damaging	1.00
R4232:Wnk1	UTSW	6	119949261	missense	possibly damaging	0.90
R4422:Wnk1	UTSW	6	119953895	missense	probably benign	0.41
R4423:Wnk1	UTSW	6	119926426	missense	probably damaging	1.00
R4572:Wnk1	UTSW	6	119951911	missense	possibly damaging	0.49
R4704:Wnk1	UTSW	6	119965744	missense	possibly damaging	0.83
R4755:Wnk1	UTSW	6	119963470	missense	probably damaging	0.98
R4812:Wnk1	UTSW	6	119952771	missense	probably benign	0.16
R4822:Wnk1	UTSW	6	119962438	missense	probably benign	0.02
R4879:Wnk1	UTSW	6	119949377	missense	probably damaging	1.00
R4970:Wnk1	UTSW	6	119965735	intron	probably benign
R5002:Wnk1	UTSW	6	119937963	missense	probably benign	0.13
R5037:Wnk1	UTSW	6	119965735	intron	probably benign
R5152:Wnk1	UTSW	6	120002280	missense	possibly damaging	0.88
R5257:Wnk1	UTSW	6	120037188	missense	probably benign	0.00
R5354:Wnk1	UTSW	6	119968523	missense	probably benign	0.01
R5421:Wnk1	UTSW	6	119952818	missense	probably damaging	1.00
R5564:Wnk1	UTSW	6	119948891	unclassified	probably benign
R5600:Wnk1	UTSW	6	119949358	missense	probably damaging	1.00
R5847:Wnk1	UTSW	6	119992408	missense	probably damaging	1.00
R6083:Wnk1	UTSW	6	120037601	missense	probably damaging	0.99
R6110:Wnk1	UTSW	6	119972997	intron	probably benign
R6128:Wnk1	UTSW	6	119963786	splice site	probably null
R6237:Wnk1	UTSW	6	119952767	missense	probably damaging	1.00
R6341:Wnk1	UTSW	6	119948585	missense	probably damaging	1.00
R6467:Wnk1	UTSW	6	119962955	missense	probably benign	0.12
R6696:Wnk1	UTSW	6	119948282	missense	probably damaging	1.00
R6888:Wnk1	UTSW	6	119948781	missense	probably benign	0.26
R6923:Wnk1	UTSW	6	119965678	intron	probably benign
R7024:Wnk1	UTSW	6	119965726	intron	probably benign
R7072:Wnk1	UTSW	6	119937861	missense	unknown
R7087:Wnk1	UTSW	6	120037530	missense	possibly damaging	0.94
R7102:Wnk1	UTSW	6	119948307	missense	unknown
R7134:Wnk1	UTSW	6	119926428	missense	unknown
R7137:Wnk1	UTSW	6	120038212	unclassified	probably benign
R7142:Wnk1	UTSW	6	119949279	missense	probably benign	0.09
R7174:Wnk1	UTSW	6	119970978	missense	probably damaging	1.00
R7205:Wnk1	UTSW	6	119943878	splice site	probably null
R7498:Wnk1	UTSW	6	119927196	missense	unknown
R7599:Wnk1	UTSW	6	119929828	missense	possibly damaging	0.83
R7615:Wnk1	UTSW	6	119932738	missense	probably benign	0.27
R7799:Wnk1	UTSW	6	119949176	missense	probably benign	0.04
R7979:Wnk1	UTSW	6	120037448	missense	probably damaging	1.00
R8075:Wnk1	UTSW	6	119932714	missense	probably damaging	0.99
R8331:Wnk1	UTSW	6	119953833	missense	probably benign	0.09
R8343:Wnk1	UTSW	6	119963493	missense	probably damaging	0.99
R8348:Wnk1	UTSW	6	119929999	splice site	probably null
R8359:Wnk1	UTSW	6	119992447	missense	probably damaging	1.00
R8424:Wnk1	UTSW	6	119934427	missense	unknown
R8519:Wnk1	UTSW	6	119950083	missense	probably damaging	1.00
R8930:Wnk1	UTSW	6	119963265	missense	probably damaging	0.99
R8932:Wnk1	UTSW	6	119963265	missense	probably damaging	0.99
R8933:Wnk1	UTSW	6	120036998	missense	probably damaging	1.00
R9005:Wnk1	UTSW	6	119962432	missense	probably damaging	1.00
R9147:Wnk1	UTSW	6	119948670	missense	unknown
R9148:Wnk1	UTSW	6	119948670	missense	unknown
R9354:Wnk1	UTSW	6	119965699	missense	unknown
R9379:Wnk1	UTSW	6	119951717	missense	probably damaging	1.00
X0064:Wnk1	UTSW	6	120037032	missense	possibly damaging	0.93
Z1177:Wnk1	UTSW	6	119948205	missense	unknown

Predicted Primers

PCR Primer
(F):5'- GTTTATTCAGCAACAAACTCATCCC -3'
(R):5'- ATGTGCCATCTTGACCAGC -3'

Sequencing Primer
(F):5'- TGTGGGTAGTAAAAACACATTGGTTG -3'
(R):5'- GTGCCATCTTGACCAGCTTATTTAG -3'

Posted On

2019-06-26