Incidental Mutation 'R7219:Try5'
ID561652
Institutional Source Beutler Lab
Gene Symbol Try5
Ensembl Gene ENSMUSG00000036938
Gene Nametrypsin 5
Synonyms1810049H19Rik, Tc
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.076) question?
Stock #R7219 (G1)
Quality Score225.009
Status Validated
Chromosome6
Chromosomal Location41311233-41316841 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to A at 41311703 bp
ZygosityHeterozygous
Amino Acid Change Aspartic acid to Valine at position 194 (D194V)
Ref Sequence ENSEMBL: ENSMUSP00000064498 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000064324] [ENSMUST00000173916]
Predicted Effect probably damaging
Transcript: ENSMUST00000064324
AA Change: D194V

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000064498
Gene: ENSMUSG00000036938
AA Change: D194V

DomainStartEndE-ValueType
low complexity region 4 16 N/A INTRINSIC
Tryp_SPc 23 239 1.47e-104 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000173916
AA Change: D86V

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000133640
Gene: ENSMUSG00000036938
AA Change: D86V

DomainStartEndE-ValueType
Tryp_SPc 1 131 1.21e-27 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.7%
  • 20x: 99.0%
Validation Efficiency 97% (77/79)
Allele List at MGI
Other mutations in this stock
Total: 79 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930562C15Rik T C 16: 4,849,644 S300P unknown Het
Abhd17a T A 10: 80,584,174 K226* probably null Het
Alkbh7 T A 17: 56,998,508 H108Q probably damaging Het
Ankrd44 A C 1: 54,766,910 H112Q probably damaging Het
Capn3 A G 2: 120,503,454 E790G probably damaging Het
Cd47 C A 16: 49,908,077 N330K possibly damaging Het
Cd55 A G 1: 130,462,606 S22P possibly damaging Het
Cdc25a A G 9: 109,889,086 I373V probably damaging Het
Cfap43 T C 19: 47,791,473 I514V probably benign Het
Cfap73 A T 5: 120,630,135 M186K probably benign Het
Chd9 A G 8: 91,001,766 D1270G unknown Het
Ciita A G 16: 10,512,257 T802A probably benign Het
Dlgap2 A G 8: 14,743,296 E430G probably benign Het
Dlx1 C A 2: 71,530,169 S59* probably null Het
Dnaaf3 T C 7: 4,528,077 N119S probably damaging Het
Dnah11 T G 12: 118,041,095 I2164L possibly damaging Het
Dnah11 T C 12: 118,126,889 K1079R probably benign Het
Dnah12 C T 14: 26,854,880 T3029I probably damaging Het
Dppa3 A T 6: 122,629,959 Y136F probably damaging Het
Enox1 T A 14: 77,720,844 M611K probably benign Het
Fam149a A T 8: 45,350,563 I378N possibly damaging Het
Fam208b T A 13: 3,590,521 L205F probably damaging Het
Farp2 T C 1: 93,560,318 F89S probably damaging Het
Fbn2 C T 18: 58,053,027 V1750M probably benign Het
Frs3 A G 17: 47,702,695 T181A probably damaging Het
Heatr4 T A 12: 83,957,870 I726F possibly damaging Het
Ighg1 T G 12: 113,326,596 E375A Het
Ikzf4 G T 10: 128,634,383 Q476K possibly damaging Het
Kcnh1 G T 1: 192,505,637 C829F probably benign Het
Krtap19-4 T C 16: 88,884,909 Y53C unknown Het
Lor C A 3: 92,081,398 G194C unknown Het
Lrp1 A C 10: 127,557,228 D2720E probably benign Het
Lrp4 A G 2: 91,492,023 Y1068C probably damaging Het
Mbnl1 A G 3: 60,603,823 N67D probably benign Het
Mrpl21 A G 19: 3,286,998 E123G probably benign Het
Mst1 A T 9: 108,081,286 D65V probably damaging Het
Myo15b T A 11: 115,877,095 probably null Het
Myo5a A G 9: 75,120,770 Y79C probably damaging Het
Oas1c A T 5: 120,802,892 W279R probably damaging Het
Olfr1283 A T 2: 111,369,537 I302L probably benign Het
Olfr1291-ps1 A T 2: 111,500,187 M312L probably benign Het
Olfr561 A G 7: 102,781,706 I77V probably benign Het
Pcdh9 C T 14: 93,015,780 G1149D possibly damaging Het
Pcid2 G A 8: 13,079,907 T283I probably benign Het
Pdhx A G 2: 103,028,415 V348A probably benign Het
Pi16 C A 17: 29,319,234 P7Q possibly damaging Het
Psmb3 T A 11: 97,711,197 M131K probably null Het
Raph1 A G 1: 60,502,873 Y309H unknown Het
Rasgrf1 A G 9: 89,984,288 N593S probably damaging Het
Rbm38 A C 2: 173,022,197 E53A possibly damaging Het
Rufy3 A G 5: 88,649,856 T631A probably benign Het
Sbno1 A T 5: 124,405,659 D272E probably benign Het
Scamp1 T A 13: 94,224,907 Y207F probably damaging Het
Scn8a T A 15: 100,969,103 S263R probably damaging Het
Setbp1 T G 18: 78,755,745 T1407P probably damaging Het
Skor2 T C 18: 76,860,401 L606P possibly damaging Het
Slc35f6 A G 5: 30,657,452 N241S probably benign Het
Slc36a2 T G 11: 55,168,918 D247A probably benign Het
Smim10l1 T A 6: 133,107,932 F87L unknown Het
Son T C 16: 91,665,001 S2265P unknown Het
Spta1 A G 1: 174,222,637 N1748D probably damaging Het
Sptbn2 A G 19: 4,724,173 D84G probably damaging Het
Tbc1d24 G A 17: 24,185,292 R293C probably damaging Het
Tex15 A G 8: 33,546,240 T65A probably benign Het
Thyn1 A G 9: 27,005,210 Y97C probably damaging Het
Tmem64 T C 4: 15,266,700 L250P probably damaging Het
Tnfrsf9 A T 4: 150,935,534 K217N probably damaging Het
Tnxb A G 17: 34,679,065 T896A probably benign Het
Trpm1 T A 7: 64,204,585 I285N possibly damaging Het
U2surp G A 9: 95,490,162 R316* probably null Het
Ubr2 G T 17: 46,935,434 S1618* probably null Het
Ugp2 T C 11: 21,323,271 I449M probably damaging Het
Umodl1 A G 17: 30,982,262 probably null Het
Vmn2r31 C T 7: 7,387,106 V538I probably benign Het
Vmn2r31 A T 7: 7,394,398 M287K probably damaging Het
Wwc2 A G 8: 47,858,884 V748A unknown Het
Zfp800 A C 6: 28,243,663 H434Q probably benign Het
Zfp869 A G 8: 69,706,706 C406R probably damaging Het
Zhx1 A G 15: 58,054,337 V171A probably benign Het
Other mutations in Try5
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01837:Try5 APN 6 41313424 missense probably benign 0.31
IGL01885:Try5 APN 6 41311738 missense possibly damaging 0.93
IGL02652:Try5 APN 6 41311408 missense probably benign 0.01
R1955:Try5 UTSW 6 41311769 missense probably benign 0.17
R2014:Try5 UTSW 6 41314651 splice site probably null
R2015:Try5 UTSW 6 41314651 splice site probably null
R2848:Try5 UTSW 6 41313476 missense probably benign 0.01
R4227:Try5 UTSW 6 41313467 missense possibly damaging 0.65
R4685:Try5 UTSW 6 41311299 missense possibly damaging 0.59
R4816:Try5 UTSW 6 41313415 missense probably benign 0.18
R5230:Try5 UTSW 6 41312378 missense probably benign 0.19
R5658:Try5 UTSW 6 41312427 missense probably damaging 1.00
R6518:Try5 UTSW 6 41314679 missense probably benign
R6910:Try5 UTSW 6 41311799 missense possibly damaging 0.62
R6913:Try5 UTSW 6 41311332 missense probably damaging 1.00
R7242:Try5 UTSW 6 41313454 missense probably benign 0.09
R7444:Try5 UTSW 6 41311365 missense probably benign 0.00
R7575:Try5 UTSW 6 41311814 missense probably benign 0.05
R7585:Try5 UTSW 6 41311814 missense probably benign 0.14
R8011:Try5 UTSW 6 41313487 missense probably benign 0.12
Predicted Primers PCR Primer
(F):5'- ATGCCATTCTAGCCATGCTTTG -3'
(R):5'- TGAAGGATGCTCCAGGAACC -3'

Sequencing Primer
(F):5'- ATGCTTTGCCTCTCTTGGTTAG -3'
(R):5'- GGAACCATAGAGTTTCTCCACTGG -3'
Posted On2019-06-26