|Institutional Source||Beutler Lab|
|Gene Name||CCR4-NOT transcription complex, subunit 9|
|Synonyms||FL10, Rqcd1, 2610007F23Rik|
|Is this an essential gene?||Essential (E-score: 1.000)|
|Stock #||R7224 (G1)|
|Chromosomal Location||74506058-74530842 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to G at 74517229 bp (GRCm38)|
|Amino Acid Change||Threonine to Alanine at position 62 (T62A)|
|Ref Sequence||ENSEMBL: ENSMUSP00000084466 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000087215] [ENSMUST00000189257]|
AA Change: T62A
PolyPhen 2 Score 0.164 (Sensitivity: 0.92; Specificity: 0.87)
AA Change: T62A
|Coding Region Coverage||
|Validation Efficiency||99% (79/80)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the highly conserved RCD1 protein family. The encoded protein is a transcriptional cofactor and a core protein of the CCR4-NOT complex. It may be involved in signal transduction as well as retinoic acid-regulated cell differentiation and development. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2012]
PHENOTYPE: Embryos homozygous for an ENU-induced allele exhibit abnormal nervous system and brain morphology, cranioedema, and caudal body truncation. Mice carrying a targeted allele exhibit embryonic lethality. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Cnot9||
(F):5'- CCTAAAGCCTGTGCCTACTG -3'
(R):5'- TTTAAGGCCTGCTTCTCAGATC -3'
(F):5'- TACTGCACTAGCCCAAGTGG -3'
(R):5'- CTGGAACTCATTCTGTAGACCAGG -3'