Mutagenetix > Incidental Mutation

Incidental Mutation 'R0576:Tas2r129'

56229

Institutional Source

Beutler Lab

Gene Symbol

Tas2r129

Ensembl Gene

ENSMUSG00000063762

Gene Name

taste receptor, type 2, member 129

Synonyms

Tas2r29, mt2r60, mGR29, T2R29

MMRRC Submission

038766-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.048) question?

Stock #

R0576 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

132928065-132929027 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 132928497 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 145 (T145A)

Ref Sequence

ENSEMBL: ENSMUSP00000067640 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000070991]

AlphaFold

Q7M709

Predicted Effect

probably benign
Transcript: ENSMUST00000070991
AA Change: T145A

PolyPhen 2 Score 0.383 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000067640
Gene: ENSMUSG00000063762
AA Change: T145A

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	302	2.3e-74	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.7%
3x: 99.1%
10x: 97.6%
20x: 94.9%

Validation Efficiency

92% (47/51)

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ccdc77	T	A	6: 120,308,809 (GRCm39)	L335F	probably benign	Het
Ccr3	T	A	9: 123,829,046 (GRCm39)	F127Y	probably damaging	Het
Cfap43	T	C	19: 47,785,579 (GRCm39)	N437S	probably benign	Het
Cfh	A	G	1: 140,064,553 (GRCm39)	V365A	probably damaging	Het
Copg1	T	A	6: 87,874,945 (GRCm39)	V380D	probably damaging	Het
Cxxc1	T	C	18: 74,353,256 (GRCm39)	I497T	possibly damaging	Het
Disp3	G	A	4: 148,326,047 (GRCm39)	T1237I	possibly damaging	Het
Dnaaf9	A	T	2: 130,555,390 (GRCm39)	F839L	probably benign	Het
Dnah7a	A	T	1: 53,675,246 (GRCm39)	F360L	probably benign	Het
Dnhd1	C	T	7: 105,363,252 (GRCm39)	A3938V	probably damaging	Het
Eif4g1	A	G	16: 20,502,818 (GRCm39)	D1000G	probably damaging	Het
Emsy	A	T	7: 98,242,983 (GRCm39)	V1052D	probably damaging	Het
Ep400	A	G	5: 110,858,959 (GRCm39)		probably benign	Het
Fa2h	T	C	8: 112,082,779 (GRCm39)	H146R	probably damaging	Het
Gad1	G	A	2: 70,424,996 (GRCm39)	C430Y	probably benign	Het
Gtse1	T	C	15: 85,753,252 (GRCm39)	S456P	probably damaging	Het
Gucy2g	T	C	19: 55,187,202 (GRCm39)	T1073A	probably damaging	Het
Hectd2	T	G	19: 36,562,897 (GRCm39)	N3K	probably benign	Het
Hmcn1	A	T	1: 150,525,768 (GRCm39)	C3318*	probably null	Het
Lipo2	C	T	19: 33,726,824 (GRCm39)	S71N	probably benign	Het
Mynn	G	T	3: 30,661,217 (GRCm39)	D100Y	probably damaging	Het
Myo16	G	A	8: 10,612,318 (GRCm39)		probably null	Het
Npr2	G	T	4: 43,640,947 (GRCm39)	K384N	probably benign	Het
Nrde2	A	G	12: 100,098,492 (GRCm39)	V725A	possibly damaging	Het
Or11h23	T	A	14: 50,948,661 (GRCm39)	S291R	probably damaging	Het
Or2l13	A	G	16: 19,305,938 (GRCm39)	M117V	probably damaging	Het
Otud7a	C	T	7: 63,335,266 (GRCm39)	P101S	possibly damaging	Het
Pcdhb7	T	A	18: 37,475,410 (GRCm39)	L182Q	probably benign	Het
Pdss1	A	G	2: 22,805,425 (GRCm39)		probably null	Het
Ppargc1b	T	A	18: 61,444,512 (GRCm39)	H233L	probably damaging	Het
Ppm1b	A	G	17: 85,320,987 (GRCm39)		probably null	Het
Prdm14	A	T	1: 13,195,949 (GRCm39)	S37R	possibly damaging	Het
Prss45	A	G	9: 110,667,497 (GRCm39)	T39A	probably benign	Het
Qars1	T	C	9: 108,392,161 (GRCm39)		probably benign	Het
Rxfp2	T	G	5: 149,961,712 (GRCm39)	H77Q	probably benign	Het
Scd4	A	G	19: 44,329,685 (GRCm39)	M219V	probably benign	Het
Sec24b	G	T	3: 129,834,985 (GRCm39)	P71Q	probably benign	Het
Snd1	T	G	6: 28,886,576 (GRCm39)	V861G	probably benign	Het
Sspo	A	G	6: 48,441,876 (GRCm39)		probably null	Het
Tbc1d31	T	A	15: 57,833,120 (GRCm39)	I953N	possibly damaging	Het
Tlr4	A	G	4: 66,757,732 (GRCm39)	N175S	probably benign	Het
Tspyl4	A	G	10: 34,174,518 (GRCm39)	N337D	probably damaging	Het
Ttn	A	T	2: 76,642,545 (GRCm39)	L13330H	probably damaging	Het
Usp33	T	A	3: 152,089,756 (GRCm39)	Y765*	probably null	Het
Vmn2r59	T	A	7: 41,696,529 (GRCm39)	Y71F	probably benign	Het
Zbed6	A	G	1: 133,585,576 (GRCm39)	F587S	probably benign	Het
Zfhx4	T	C	3: 5,467,161 (GRCm39)	S2465P	probably damaging	Het

Other mutations in Tas2r129

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02227:Tas2r129	APN	6	132,928,357 (GRCm39)	nonsense	probably null
R0389:Tas2r129	UTSW	6	132,928,159 (GRCm39)	missense	probably benign	0.26
R0443:Tas2r129	UTSW	6	132,928,159 (GRCm39)	missense	probably benign	0.26
R0487:Tas2r129	UTSW	6	132,928,907 (GRCm39)	missense	probably benign	0.31
R0487:Tas2r129	UTSW	6	132,928,906 (GRCm39)	missense	probably benign	0.26
R1253:Tas2r129	UTSW	6	132,928,999 (GRCm39)	missense	probably benign	0.11
R3124:Tas2r129	UTSW	6	132,928,411 (GRCm39)	missense	probably damaging	1.00
R4493:Tas2r129	UTSW	6	132,928,317 (GRCm39)	missense	probably benign	0.31
R4494:Tas2r129	UTSW	6	132,928,317 (GRCm39)	missense	probably benign	0.31
R4591:Tas2r129	UTSW	6	132,928,574 (GRCm39)	missense	probably benign	0.10
R6156:Tas2r129	UTSW	6	132,928,455 (GRCm39)	missense	probably benign	0.31
R6495:Tas2r129	UTSW	6	132,928,128 (GRCm39)	missense	probably benign	0.00
R7600:Tas2r129	UTSW	6	132,928,137 (GRCm39)	nonsense	probably null
R7608:Tas2r129	UTSW	6	132,928,156 (GRCm39)	missense	probably damaging	0.98
R8498:Tas2r129	UTSW	6	132,928,815 (GRCm39)	missense	probably benign	0.01
R8870:Tas2r129	UTSW	6	132,928,522 (GRCm39)	missense	probably damaging	0.98
R9276:Tas2r129	UTSW	6	132,928,576 (GRCm39)	missense	probably benign	0.12

Predicted Primers

PCR Primer
(F):5'- ACAGCCTTGGCTTTCTCCAGAATC -3'
(R):5'- GCATCTGCAAGGACATCTCTGCATC -3'

Sequencing Primer
(F):5'- GTGTCTACGCTATACACACACG -3'
(R):5'- CTTGTAATGTCTCCACAGGGAGAAG -3'

Posted On

2013-07-11