Mutagenetix > Incidental Mutation

Incidental Mutation 'R7230:Zfp58'

562432

Institutional Source

Beutler Lab

Gene Symbol

Zfp58

Ensembl Gene

ENSMUSG00000071291

Gene Name

zinc finger protein 58

Synonyms

Mfg1, Zfp817, A530094I17Rik, Mfg-1

MMRRC Submission

045302-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.116) question?

Stock #

R7230 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

67638286-67648641 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 67640082 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 136 (C136*)

Ref Sequence

ENSEMBL: ENSMUSP00000075487 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076123] [ENSMUST00000091523] [ENSMUST00000163534] [ENSMUST00000167914] [ENSMUST00000171518]

AlphaFold

P16372

Predicted Effect

probably null
Transcript: ENSMUST00000076123
AA Change: C136*

SMART Domains

Protein: ENSMUSP00000075487
Gene: ENSMUSG00000071291
AA Change: C136*

Domain	Start	End	E-Value	Type
KRAB	2	62	2.19e-29	SMART
ZnF_C2H2	106	128	4.01e-5	SMART
ZnF_C2H2	134	156	9.73e-4	SMART
ZnF_C2H2	162	184	5.67e-5	SMART
ZnF_C2H2	190	212	3.21e-4	SMART
ZnF_C2H2	218	240	2.57e-3	SMART
ZnF_C2H2	246	268	2.91e-2	SMART
ZnF_C2H2	274	296	9.73e-4	SMART
ZnF_C2H2	302	324	6.23e-2	SMART
ZnF_C2H2	330	352	1.03e-2	SMART
ZnF_C2H2	358	380	1.45e-2	SMART
ZnF_C2H2	386	408	2.99e-4	SMART
ZnF_C2H2	410	432	4.87e-4	SMART
ZnF_C2H2	438	460	2.75e-3	SMART
ZnF_C2H2	466	488	1.58e-3	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000091523
AA Change: C136*

SMART Domains

Protein: ENSMUSP00000089108
Gene: ENSMUSG00000071291
AA Change: C136*

Domain	Start	End	E-Value	Type
KRAB	2	62	2.19e-29	SMART
ZnF_C2H2	106	128	4.01e-5	SMART
ZnF_C2H2	134	156	9.73e-4	SMART
ZnF_C2H2	162	184	5.67e-5	SMART
ZnF_C2H2	190	212	3.21e-4	SMART
ZnF_C2H2	218	240	2.57e-3	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000163534
AA Change: C136*

SMART Domains

Protein: ENSMUSP00000129177
Gene: ENSMUSG00000071291
AA Change: C136*

Domain	Start	End	E-Value	Type
KRAB	2	62	2.19e-29	SMART
ZnF_C2H2	106	128	4.01e-5	SMART
ZnF_C2H2	134	156	9.73e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000167914

SMART Domains

Protein: ENSMUSP00000137830
Gene: ENSMUSG00000097565

Domain	Start	End	E-Value	Type
KRAB	5	65	4.41e-34	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000171518
AA Change: C136*

SMART Domains

Protein: ENSMUSP00000132285
Gene: ENSMUSG00000071291
AA Change: C136*

Domain	Start	End	E-Value	Type
KRAB	2	62	2.19e-29	SMART
ZnF_C2H2	106	128	4.01e-5	SMART
ZnF_C2H2	134	156	9.73e-4	SMART
ZnF_C2H2	162	184	5.67e-5	SMART
ZnF_C2H2	190	212	3.21e-4	SMART
ZnF_C2H2	218	240	2.57e-3	SMART
ZnF_C2H2	246	268	2.91e-2	SMART
ZnF_C2H2	274	296	9.73e-4	SMART
ZnF_C2H2	302	324	6.23e-2	SMART
ZnF_C2H2	330	352	1.03e-2	SMART
ZnF_C2H2	358	380	1.45e-2	SMART
ZnF_C2H2	386	408	2.99e-4	SMART
ZnF_C2H2	410	432	4.87e-4	SMART
ZnF_C2H2	438	460	2.75e-3	SMART
ZnF_C2H2	466	488	1.58e-3	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.4%

Validation Efficiency

100% (50/50)

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc8	A	C	7: 45,766,812 (GRCm39)	D989E	probably benign	Het
Adad1	A	G	3: 37,119,315 (GRCm39)	Y132C	probably damaging	Het
Adam33	A	T	2: 130,895,483 (GRCm39)	C579S	probably damaging	Het
Adam6a	A	C	12: 113,509,202 (GRCm39)	Q525P	probably damaging	Het
Alpk3	C	T	7: 80,743,042 (GRCm39)	P953L	probably damaging	Het
Arb2a	G	A	13: 77,907,591 (GRCm39)	E5K	probably damaging	Het
Atat1	A	G	17: 36,220,331 (GRCm39)	S54P	probably damaging	Het
Bpgm	A	G	6: 34,464,502 (GRCm39)	E73G	possibly damaging	Het
Cab39	T	A	1: 85,775,880 (GRCm39)		probably null	Het
Ccdc162	A	G	10: 41,554,809 (GRCm39)	L285P	probably damaging	Het
Ccdc30	T	C	4: 119,196,979 (GRCm39)	E429G	possibly damaging	Het
Cct3	C	T	3: 88,220,567 (GRCm39)	R260W	probably damaging	Het
Chd1	C	A	17: 15,927,199 (GRCm39)		probably null	Het
Cxcr4	T	G	1: 128,517,527 (GRCm39)	T45P	probably damaging	Het
Disp2	G	T	2: 118,622,286 (GRCm39)	R1006L	probably damaging	Het
Dlec1	T	G	9: 118,953,606 (GRCm39)		probably null	Het
Dram2	T	G	3: 106,480,294 (GRCm39)	Y202*	probably null	Het
Etl4	C	A	2: 20,802,799 (GRCm39)	T1035K	probably damaging	Het
F5	T	C	1: 164,012,522 (GRCm39)	F479L	probably benign	Het
Frrs1l	C	A	4: 56,972,372 (GRCm39)	G110W	probably damaging	Het
Gpbp1l1	T	A	4: 116,445,807 (GRCm39)	I303N	probably damaging	Het
Grik5	A	G	7: 24,722,495 (GRCm39)	F538S	probably damaging	Het
Hgsnat	C	A	8: 26,444,860 (GRCm39)		probably null	Het
Hs2st1	T	C	3: 144,140,307 (GRCm39)	D338G	probably benign	Het
Impdh1	T	C	6: 29,206,062 (GRCm39)		probably null	Het
Ipo9	T	C	1: 135,334,496 (GRCm39)		probably benign	Het
Kdm4b	T	G	17: 56,676,155 (GRCm39)	L220R	probably damaging	Het
Map1a	T	A	2: 121,131,299 (GRCm39)	F705Y	probably damaging	Het
Med22	C	T	2: 26,798,223 (GRCm39)	D99N	probably benign	Het
Muc6	T	C	7: 141,235,479 (GRCm39)	Y519C	probably damaging	Het
Myt1l	A	G	12: 29,833,873 (GRCm39)	I25M	probably damaging	Het
Ncam1	T	A	9: 49,421,123 (GRCm39)	I731F	probably benign	Het
Nlrp4f	T	A	13: 65,342,715 (GRCm39)	H310L	probably benign	Het
Or2ag1b	A	T	7: 106,288,731 (GRCm39)	M69K	possibly damaging	Het
Or2ag2b	C	A	7: 106,417,386 (GRCm39)	T32K	possibly damaging	Het
Or4f14	T	A	2: 111,742,906 (GRCm39)	Y123F	probably damaging	Het
Or4k40	A	T	2: 111,251,261 (GRCm39)	F12I	probably damaging	Het
Prl8a6	T	A	13: 27,617,021 (GRCm39)	Y223F	probably benign	Het
Prss39	A	G	1: 34,541,228 (GRCm39)	D244G	probably damaging	Het
Ptx4	A	T	17: 25,342,077 (GRCm39)	Q184L	possibly damaging	Het
Slc26a1	A	T	5: 108,819,611 (GRCm39)	D545E	probably damaging	Het
Slc7a12	T	C	3: 14,570,441 (GRCm39)	S398P	probably damaging	Het
Slc9a4	T	C	1: 40,639,931 (GRCm39)	V241A	probably damaging	Het
Snw1	T	C	12: 87,511,324 (GRCm39)	D109G	probably damaging	Het
Syne2	T	A	12: 75,980,674 (GRCm39)	I1477K	probably benign	Het
Timd4	A	T	11: 46,701,691 (GRCm39)	Y18F	probably benign	Het
Tmprss2	A	G	16: 97,379,797 (GRCm39)	Y168H	probably benign	Het
Ttn	A	T	2: 76,569,044 (GRCm39)	I27283K	probably damaging	Het
Tulp4	C	T	17: 6,282,055 (GRCm39)	H695Y	probably damaging	Het
Vasn	C	T	16: 4,467,486 (GRCm39)	R478C	probably benign	Het

Other mutations in Zfp58

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00091:Zfp58	APN	13	67,639,114 (GRCm39)	missense	probably benign	0.29
IGL02618:Zfp58	APN	13	67,639,475 (GRCm39)	missense	possibly damaging	0.92
IGL03188:Zfp58	APN	13	67,639,528 (GRCm39)	missense	probably benign	0.03
R0535:Zfp58	UTSW	13	67,640,201 (GRCm39)	nonsense	probably null
R1470:Zfp58	UTSW	13	67,640,144 (GRCm39)	missense	possibly damaging	0.71
R1470:Zfp58	UTSW	13	67,640,144 (GRCm39)	missense	possibly damaging	0.71
R1750:Zfp58	UTSW	13	67,639,598 (GRCm39)	nonsense	probably null
R1862:Zfp58	UTSW	13	67,639,307 (GRCm39)	missense	probably damaging	1.00
R2697:Zfp58	UTSW	13	67,639,124 (GRCm39)	missense	probably damaging	1.00
R3031:Zfp58	UTSW	13	67,640,231 (GRCm39)	missense	probably benign	0.06
R3033:Zfp58	UTSW	13	67,639,741 (GRCm39)	missense	probably damaging	1.00
R4200:Zfp58	UTSW	13	67,639,440 (GRCm39)	missense	probably benign	0.25
R5827:Zfp58	UTSW	13	67,639,412 (GRCm39)	missense	probably damaging	0.99
R6723:Zfp58	UTSW	13	67,642,192 (GRCm39)	missense	probably damaging	1.00
R7890:Zfp58	UTSW	13	67,640,114 (GRCm39)	missense	possibly damaging	0.95
R9189:Zfp58	UTSW	13	67,640,035 (GRCm39)	missense	possibly damaging	0.93
R9338:Zfp58	UTSW	13	67,639,394 (GRCm39)	missense	probably benign	0.43
R9477:Zfp58	UTSW	13	67,640,158 (GRCm39)	missense	probably damaging	1.00
R9587:Zfp58	UTSW	13	67,639,823 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TCCCCAGAATGACGCAATTTA -3'
(R):5'- AATGTAAAGATTTCAGCAAGGCC -3'

Sequencing Primer
(F):5'- ACATTCTGCACACTTGTAGGG -3'
(R):5'- GGGAGAAAGCCTTCAAGT -3'

Posted On

2019-06-26