Mutagenetix > Incidental Mutations

Incidental Mutation 'R7234:Olfr620'

562702

Institutional Source

Beutler Lab

Gene Symbol

Olfr620

Ensembl Gene

ENSMUSG00000045132

Gene Name

olfactory receptor 620

Synonyms

MOR4-1, GA_x6K02T2PBJ9-6335095-6334154

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.087) question?

Stock #

R7234 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

103604895-103612534 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 103611882 bp

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 157 (L157P)

Ref Sequence

ENSEMBL: ENSMUSP00000061764 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000052152] [ENSMUST00000217603]

Predicted Effect

probably damaging
Transcript: ENSMUST00000052152
AA Change: L157P

PolyPhen 2 Score 0.967 (Sensitivity: 0.77; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000061764
Gene: ENSMUSG00000045132
AA Change: L157P

Domain	Start	End	E-Value	Type
Pfam:7tm_4	33	312	1.6e-137	PFAM
Pfam:7tm_1	43	294	4.5e-19	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000217603
AA Change: L157P

PolyPhen 2 Score 0.967 (Sensitivity: 0.77; Specificity: 0.95)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

100% (66/66)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930402H24Rik	C	A	2: 130,806,788	R258L	unknown	Het
Acy3	T	A	19: 3,987,758	Y88*	probably null	Het
AI606181	T	A	19: 41,593,637	I82N	unknown	Het
Akap1	T	C	11: 88,838,982	Y638C	probably damaging	Het
Angpt1	T	C	15: 42,459,725	N383D	probably benign	Het
Ank	T	C	15: 27,571,656		probably null	Het
Aoc1	C	T	6: 48,905,816	Q209*	probably null	Het
Apol7c	A	T	15: 77,525,675	L357*	probably null	Het
Atxn2l	A	G	7: 126,493,201	L958P	probably damaging	Het
Cab39l	T	A	14: 59,496,946		probably null	Het
Cdk5rap2	A	G	4: 70,376,787		probably null	Het
Cdkn3	T	C	14: 46,771,461	S204P	unknown	Het
Cds2	T	C	2: 132,304,480		probably null	Het
Cideb	C	T	14: 55,754,560	R179H	probably benign	Het
Cped1	A	G	6: 22,254,626	Q1006R	probably damaging	Het
Csmd2	A	G	4: 128,456,779	Y1547C		Het
Dicer1	A	G	12: 104,708,849	L718S	probably damaging	Het
Dyrk2	T	C	10: 118,860,231	H374R	possibly damaging	Het
Edem2	A	T	2: 155,710,966	Y283N	probably benign	Het
Ero1lb	A	T	13: 12,600,314	S345C	possibly damaging	Het
Fam83g	T	C	11: 61,702,516	V292A	possibly damaging	Het
Farp2	A	G	1: 93,580,119	D513G	possibly damaging	Het
Fbxl5	A	G	5: 43,758,220	W617R	probably benign	Het
Fzd7	G	A	1: 59,483,284	V109M	probably damaging	Het
Gbp5	A	T	3: 142,521,137	H583L	probably benign	Het
Gm10306	T	A	4: 94,556,795	L84M	unknown	Het
Gm11756	G	T	4: 73,917,571	L219M	probably benign	Het
Gsap	A	G	5: 21,186,435	T25A	probably benign	Het
Hectd4	G	T	5: 121,329,073	R2466L	possibly damaging	Het
Ide	C	A	19: 37,290,785	C557F		Het
Igdcc4	T	A	9: 65,135,468	C1234*	probably null	Het
Ints4	A	G	7: 97,530,300	I701V	probably benign	Het
Kalrn	T	A	16: 34,176,422	I1467F	possibly damaging	Het
Kcnip3	C	A	2: 127,521,336	R2M	unknown	Het
Kcnrg	A	T	14: 61,608,082	E190D	unknown	Het
Klk13	T	C	7: 43,721,417	L131P	probably damaging	Het
Lhcgr	A	T	17: 88,791,931	L14Q	possibly damaging	Het
Mdm4	A	T	1: 133,011,115	D80E	probably damaging	Het
Mib2	G	T	4: 155,657,893	Q311K	probably damaging	Het
Mycbp2	A	T	14: 103,215,337	S1703T	probably damaging	Het
Naip6	A	T	13: 100,315,503	C200*	probably null	Het
Ncapd3	T	A	9: 27,050,359	I361N	probably damaging	Het
Nom1	A	G	5: 29,435,453	E259G	probably benign	Het
Olfr16	T	A	1: 172,957,106	F104I	probably damaging	Het
Plxna2	A	T	1: 194,806,390	H1658L	probably damaging	Het
Ranbp6	T	C	19: 29,812,062	T297A	possibly damaging	Het
Rap1gap	T	A	4: 137,728,540	C722*	probably null	Het
Ribc2	A	G	15: 85,135,532	K172E	probably benign	Het
Scamp5	C	A	9: 57,447,140	W77L	probably damaging	Het
Scin	T	A	12: 40,080,958	K319*	probably null	Het
Sec16a	G	T	2: 26,439,768	T745K	probably damaging	Het
Slc15a2	G	A	16: 36,757,811	A403V	probably benign	Het
Slco6c1	A	G	1: 97,125,741	V145A	probably benign	Het
Sncaip	C	T	18: 52,915,344	H951Y	probably benign	Het
Spem1	A	G	11: 69,821,804		probably null	Het
Spen	C	T	4: 141,479,135	R727Q	unknown	Het
Thra	T	C	11: 98,763,718	S305P	probably damaging	Het
Tlr1	A	G	5: 64,926,724	V170A	probably damaging	Het
Tmem260	T	A	14: 48,505,329	C388*	probably null	Het
Tmem60	T	A	5: 20,886,621	V128D	possibly damaging	Het
Tpo	G	A	12: 30,092,686	P680S	probably benign	Het
Ttc30a2	A	T	2: 75,976,196	Y657*	probably null	Het
Umodl1	A	G	17: 30,986,621	E730G	possibly damaging	Het
Vmn2r69	T	C	7: 85,407,107	T608A	probably benign	Het
Xrra1	G	A	7: 99,914,249	S481N	possibly damaging	Het
Zc3h4	G	A	7: 16,429,036	V446I	unknown	Het

Other mutations in Olfr620

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02807:Olfr620	APN	7	103611991	missense	probably benign	0.34
R0496:Olfr620	UTSW	7	103611997	missense	probably benign	0.09
R0834:Olfr620	UTSW	7	103612237	missense	probably benign	0.00
R1957:Olfr620	UTSW	7	103611411	makesense	probably null
R1958:Olfr620	UTSW	7	103611411	makesense	probably null
R2158:Olfr620	UTSW	7	103612236	missense	possibly damaging	0.66
R3797:Olfr620	UTSW	7	103611447	missense	probably benign	0.37
R5389:Olfr620	UTSW	7	103611590	nonsense	probably null
R6292:Olfr620	UTSW	7	103612179	missense	probably damaging	0.99
R6374:Olfr620	UTSW	7	103611921	missense	probably benign	0.23
R6422:Olfr620	UTSW	7	103612014	missense	probably damaging	1.00
R6586:Olfr620	UTSW	7	103611976	missense	possibly damaging	0.81
R7393:Olfr620	UTSW	7	103611991	missense	possibly damaging	0.90
R7855:Olfr620	UTSW	7	103611772	missense	possibly damaging	0.88
R7900:Olfr620	UTSW	7	103611693	missense	possibly damaging	0.82
R8159:Olfr620	UTSW	7	103612140	missense	possibly damaging	0.63
R8265:Olfr620	UTSW	7	103611841	missense	possibly damaging	0.95
R8314:Olfr620	UTSW	7	103612047	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGAGTCCACTAACAAGTTGCTG -3'
(R):5'- TGTGCACACGGTAATGGGTATC -3'

Sequencing Primer
(F):5'- TTGCTGATCACCAGAGCTAG -3'
(R):5'- TCAAGAGATCAGCCTTGATGCCTG -3'

Posted On

2019-06-26