Mutagenetix > Incidental Mutation

Incidental Mutation 'R7235:Or52n2'

562765

Institutional Source

Beutler Lab

Gene Symbol

Or52n2

Ensembl Gene

ENSMUSG00000063582

Gene Name

olfactory receptor family 52 subfamily N member 2

Synonyms

Olfr666, MOR34-1, GA_x6K02T2PBJ9-7522449-7521493

MMRRC Submission

045305-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.077) question?

Stock #

R7235 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

104541877-104542833 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 104541926 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Glutamine at position 303 (R303Q)

Ref Sequence

ENSEMBL: ENSMUSP00000148919 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000081116] [ENSMUST00000214757]

AlphaFold

Q8VH00

Predicted Effect

probably benign
Transcript: ENSMUST00000081116
AA Change: R303Q

PolyPhen 2 Score 0.015 (Sensitivity: 0.96; Specificity: 0.79)

SMART Domains

Protein: ENSMUSP00000079893
Gene: ENSMUSG00000063582
AA Change: R303Q

Domain	Start	End	E-Value	Type
Pfam:7tm_4	33	313	2.7e-104	PFAM
Pfam:7TM_GPCR_Srsx	37	209	4.1e-11	PFAM
Pfam:7tm_1	43	295	1.4e-19	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000214757
AA Change: R303Q

PolyPhen 2 Score 0.015 (Sensitivity: 0.96; Specificity: 0.79)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

98% (61/62)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ahnak	T	A	19: 8,989,852 (GRCm39)	M3712K	unknown	Het
Bri3bp	A	G	5: 125,518,748 (GRCm39)	E8G	unknown	Het
Brip1	C	T	11: 86,029,701 (GRCm39)	R611Q	possibly damaging	Het
Carm1	T	G	9: 21,498,701 (GRCm39)		probably benign	Het
Catsper4	A	T	4: 133,939,892 (GRCm39)		probably null	Het
Ccng2	C	G	5: 93,421,202 (GRCm39)	S237R	probably benign	Het
Cip2a	T	A	16: 48,821,422 (GRCm39)	L176H	probably damaging	Het
Clk4	A	G	11: 51,167,012 (GRCm39)	D330G	probably damaging	Het
Dcdc2c	A	G	12: 28,520,718 (GRCm39)	S453P		Het
Ddx46	G	A	13: 55,811,053 (GRCm39)	V550I	probably benign	Het
Dennd1a	T	C	2: 37,691,073 (GRCm39)	N676D	probably benign	Het
Dnah3	T	C	7: 119,631,893 (GRCm39)	N1365S	probably damaging	Het
Dsg1b	T	C	18: 20,532,480 (GRCm39)	V508A	probably benign	Het
Dus2	T	C	8: 106,742,587 (GRCm39)	V39A	possibly damaging	Het
Dync1li1	T	C	9: 114,544,231 (GRCm39)	V301A	possibly damaging	Het
Efcc1	T	G	6: 87,730,780 (GRCm39)	S513A	probably benign	Het
Eif3f	T	C	7: 108,537,295 (GRCm39)	V167A	possibly damaging	Het
Ephb2	A	G	4: 136,421,139 (GRCm39)	Y404H	probably damaging	Het
Eqtn	A	T	4: 94,811,936 (GRCm39)	D152E	probably damaging	Het
Ercc5	A	C	1: 44,217,363 (GRCm39)	K902T	possibly damaging	Het
Erlec1	T	A	11: 30,900,751 (GRCm39)	E139V	possibly damaging	Het
Evl	G	A	12: 108,614,719 (GRCm39)	G38R	probably damaging	Het
Fads2b	T	G	2: 85,330,563 (GRCm39)	H248P	probably damaging	Het
Foxd2	T	C	4: 114,765,468 (GRCm39)	N184S	unknown	Het
Frem3	T	C	8: 81,417,354 (GRCm39)	Y2020H	probably benign	Het
Ganc	T	C	2: 120,264,198 (GRCm39)	F384L	probably damaging	Het
Grin2a	A	G	16: 9,397,129 (GRCm39)	L986P	probably damaging	Het
Ift140	A	T	17: 25,239,619 (GRCm39)	D92V	possibly damaging	Het
Inpp5b	A	G	4: 124,645,185 (GRCm39)	K158E	probably benign	Het
Iscu	T	A	5: 113,914,943 (GRCm39)	S152T	probably benign	Het
Isl2	C	T	9: 55,451,455 (GRCm39)	T115I	probably benign	Het
Kalrn	T	A	16: 33,996,131 (GRCm39)	T1542S	probably benign	Het
Kat6a	A	G	8: 23,404,285 (GRCm39)	D454G	possibly damaging	Het
Kcnj10	T	C	1: 172,196,993 (GRCm39)	I169T	probably damaging	Het
Klk12	T	C	7: 43,422,723 (GRCm39)	S217P	probably damaging	Het
Lancl1	T	C	1: 67,077,694 (GRCm39)	N14S	probably benign	Het
Map2	A	G	1: 66,453,807 (GRCm39)	Y899C	probably damaging	Het
Msantd5f9	G	T	4: 73,835,808 (GRCm39)	L219M	probably benign	Het
Nr1h5	C	T	3: 102,856,358 (GRCm39)		probably null	Het
Nup98	T	C	7: 101,774,491 (GRCm39)	T1515A	probably damaging	Het
Obscn	A	C	11: 58,971,666 (GRCm39)	V2183G	probably damaging	Het
Osbpl8	A	G	10: 111,105,288 (GRCm39)	T248A	probably benign	Het
Pias3	T	A	3: 96,611,679 (GRCm39)	S533T	probably benign	Het
Pkd1l3	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	8: 110,350,827 (GRCm39)		probably benign	Het
Plxna1	A	G	6: 89,317,573 (GRCm39)	Y680H	probably damaging	Het
Pnliprp2	A	G	19: 58,763,659 (GRCm39)	N436S	probably benign	Het
Pold1	T	A	7: 44,191,244 (GRCm39)	M196L	probably benign	Het
Prkdc	G	A	16: 15,532,127 (GRCm39)	V1464I	probably benign	Het
Ptk2b	G	A	14: 66,394,536 (GRCm39)	Q857*	probably null	Het
Qars1	T	A	9: 108,387,331 (GRCm39)	L185Q	probably damaging	Het
Rabep1	A	G	11: 70,831,290 (GRCm39)	N859S	probably benign	Het
Rnf19b	A	G	4: 128,977,571 (GRCm39)	H156R		Het
Sart3	T	C	5: 113,891,703 (GRCm39)	Q423R	probably damaging	Het
Selplg	GTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCT	GTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCT	5: 113,957,756 (GRCm39)		probably benign	Het
Slc6a21	C	T	7: 44,930,182 (GRCm39)	H194Y	probably damaging	Het
Tcf4	G	A	18: 69,790,866 (GRCm39)	V420I	probably damaging	Het
Tjp1	T	C	7: 64,968,321 (GRCm39)	D701G	probably benign	Het
Trim42	T	C	9: 97,251,761 (GRCm39)	D46G	probably damaging	Het
Usp19	C	T	9: 108,372,123 (GRCm39)	R429*	probably null	Het
Uxs1	T	C	1: 43,804,087 (GRCm39)	N276S	probably damaging	Het
Vps50	A	G	6: 3,588,078 (GRCm39)	I684V	probably benign	Het
Yars2	T	A	16: 16,122,556 (GRCm39)	D307E	probably benign	Het
Zfp934	A	T	13: 62,665,964 (GRCm39)	C258S		Het

Other mutations in Or52n2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00326:Or52n2	APN	7	104,542,179 (GRCm39)	missense	probably damaging	1.00
IGL01016:Or52n2	APN	7	104,542,243 (GRCm39)	missense	probably damaging	1.00
IGL01577:Or52n2	APN	7	104,542,730 (GRCm39)	missense	probably benign
IGL01775:Or52n2	APN	7	104,542,499 (GRCm39)	missense	possibly damaging	0.95
IGL01932:Or52n2	APN	7	104,542,425 (GRCm39)	missense	probably damaging	0.99
IGL02559:Or52n2	APN	7	104,542,161 (GRCm39)	missense	probably benign	0.01
IGL03193:Or52n2	APN	7	104,542,263 (GRCm39)	missense	probably benign	0.31
R0359:Or52n2	UTSW	7	104,542,521 (GRCm39)	missense	probably damaging	0.99
R0494:Or52n2	UTSW	7	104,542,478 (GRCm39)	missense	probably damaging	1.00
R0680:Or52n2	UTSW	7	104,542,211 (GRCm39)	missense	probably benign	0.23
R1476:Or52n2	UTSW	7	104,542,444 (GRCm39)	nonsense	probably null
R1988:Or52n2	UTSW	7	104,542,110 (GRCm39)	missense	probably damaging	1.00
R2520:Or52n2	UTSW	7	104,542,080 (GRCm39)	missense	probably benign	0.01
R3690:Or52n2	UTSW	7	104,542,724 (GRCm39)	missense	possibly damaging	0.66
R5220:Or52n2	UTSW	7	104,542,104 (GRCm39)	missense	possibly damaging	0.94
R5322:Or52n2	UTSW	7	104,542,371 (GRCm39)	missense	probably benign	0.14
R5728:Or52n2	UTSW	7	104,542,436 (GRCm39)	missense	possibly damaging	0.88
R6865:Or52n2	UTSW	7	104,542,719 (GRCm39)	missense	probably benign	0.00
R7404:Or52n2	UTSW	7	104,542,181 (GRCm39)	missense	possibly damaging	0.61
R9146:Or52n2	UTSW	7	104,542,452 (GRCm39)	missense	probably damaging	1.00
R9569:Or52n2	UTSW	7	104,542,525 (GRCm39)	missense	possibly damaging	0.59

Predicted Primers

PCR Primer
(F):5'- CTACATCCTATACTTTGGCAGAGC -3'
(R):5'- CACATCTGTGCTATTGTGATCAC -3'

Sequencing Primer
(F):5'- ATCCTATACTTTGGCAGAGCATTCC -3'
(R):5'- ATTGTGATCACTTATGTGCCTGC -3'

Posted On

2019-06-26