Incidental Mutation 'R7235:Eif3f'
ID562766
Institutional Source Beutler Lab
Gene Symbol Eif3f
Ensembl Gene ENSMUSG00000031029
Gene Nameeukaryotic translation initiation factor 3, subunit F
SynonymsEif3s5, 0610037M02Rik
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably essential (E-score: 0.961) question?
Stock #R7235 (G1)
Quality Score225.009
Status Validated
Chromosome7
Chromosomal Location108934417-108942951 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 108938088 bp
ZygosityHeterozygous
Amino Acid Change Valine to Alanine at position 167 (V167A)
Ref Sequence ENSEMBL: ENSMUSP00000033342 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000033342]
Predicted Effect possibly damaging
Transcript: ENSMUST00000033342
AA Change: V167A

PolyPhen 2 Score 0.778 (Sensitivity: 0.85; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000033342
Gene: ENSMUSG00000031029
AA Change: V167A

DomainStartEndE-ValueType
low complexity region 2 93 N/A INTRINSIC
JAB_MPN 95 225 3.11e-42 SMART
Pfam:MitMem_reg 247 359 7e-27 PFAM
Meta Mutation Damage Score 0.4307 question?
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.6%
  • 20x: 98.9%
Validation Efficiency 98% (61/62)
Allele List at MGI
Other mutations in this stock
Total: 63 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4833423E24Rik T G 2: 85,500,219 H248P probably damaging Het
Ahnak T A 19: 9,012,488 M3712K unknown Het
Bri3bp A G 5: 125,441,684 E8G unknown Het
Brip1 C T 11: 86,138,875 R611Q possibly damaging Het
C330027C09Rik T A 16: 49,001,059 L176H probably damaging Het
Carm1 T G 9: 21,587,405 probably benign Het
Catsper4 A T 4: 134,212,581 probably null Het
Ccng2 C G 5: 93,273,343 S237R probably benign Het
Clk4 A G 11: 51,276,185 D330G probably damaging Het
Dcdc2c A G 12: 28,470,719 S453P Het
Ddx46 G A 13: 55,663,240 V550I probably benign Het
Dennd1a T C 2: 37,801,061 N676D probably benign Het
Dnah3 T C 7: 120,032,670 N1365S probably damaging Het
Dsg1b T C 18: 20,399,423 V508A probably benign Het
Dus2 T C 8: 106,015,955 V39A possibly damaging Het
Dync1li1 T C 9: 114,715,163 V301A possibly damaging Het
Efcc1 T G 6: 87,753,798 S513A probably benign Het
Ephb2 A G 4: 136,693,828 Y404H probably damaging Het
Eqtn A T 4: 94,923,699 D152E probably damaging Het
Ercc5 A C 1: 44,178,203 K902T possibly damaging Het
Erlec1 T A 11: 30,950,751 E139V possibly damaging Het
Evl G A 12: 108,648,460 G38R probably damaging Het
Foxd2 T C 4: 114,908,271 N184S unknown Het
Frem3 T C 8: 80,690,725 Y2020H probably benign Het
Ganc T C 2: 120,433,717 F384L probably damaging Het
Gm11756 G T 4: 73,917,571 L219M probably benign Het
Grin2a A G 16: 9,579,265 L986P probably damaging Het
Ift140 A T 17: 25,020,645 D92V possibly damaging Het
Inpp5b A G 4: 124,751,392 K158E probably benign Het
Iscu T A 5: 113,776,882 S152T probably benign Het
Isl2 C T 9: 55,544,171 T115I probably benign Het
Kalrn T A 16: 34,175,761 T1542S probably benign Het
Kat6a A G 8: 22,914,269 D454G possibly damaging Het
Kcnj10 T C 1: 172,369,426 I169T probably damaging Het
Klk12 T C 7: 43,773,299 S217P probably damaging Het
Lancl1 T C 1: 67,038,535 N14S probably benign Het
Map2 A G 1: 66,414,648 Y899C probably damaging Het
Nr1h5 C T 3: 102,949,042 probably null Het
Nup98 T C 7: 102,125,284 T1515A probably damaging Het
Obscn A C 11: 59,080,840 V2183G probably damaging Het
Olfr666 C T 7: 104,892,719 R303Q probably benign Het
Osbpl8 A G 10: 111,269,427 T248A probably benign Het
Pias3 T A 3: 96,704,363 S533T probably benign Het
Pkd1l3 GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA 8: 109,624,195 probably benign Het
Plxna1 A G 6: 89,340,591 Y680H probably damaging Het
Pnliprp2 A G 19: 58,775,227 N436S probably benign Het
Pold1 T A 7: 44,541,820 M196L probably benign Het
Prkdc G A 16: 15,714,263 V1464I probably benign Het
Ptk2b G A 14: 66,157,087 Q857* probably null Het
Qars T A 9: 108,510,132 L185Q probably damaging Het
Rabep1 A G 11: 70,940,464 N859S probably benign Het
Rnf19b A G 4: 129,083,778 H156R Het
Sart3 T C 5: 113,753,642 Q423R probably damaging Het
Selplg GTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCT GTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCT 5: 113,819,695 probably benign Het
Slc6a21 C T 7: 45,280,758 H194Y probably damaging Het
Tcf4 G A 18: 69,657,795 V420I probably damaging Het
Tjp1 T C 7: 65,318,573 D701G probably benign Het
Trim42 T C 9: 97,369,708 D46G probably damaging Het
Usp19 C T 9: 108,494,924 R429* probably null Het
Uxs1 T C 1: 43,764,927 N276S probably damaging Het
Vps50 A G 6: 3,588,078 I684V probably benign Het
Yars2 T A 16: 16,304,692 D307E probably benign Het
Zfp934 A T 13: 62,518,150 C258S Het
Other mutations in Eif3f
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02690:Eif3f APN 7 108934718 missense probably damaging 1.00
IGL02821:Eif3f APN 7 108934674 unclassified probably benign
IGL02821:Eif3f APN 7 108934675 unclassified probably benign
R0010:Eif3f UTSW 7 108941005 missense possibly damaging 0.93
R1054:Eif3f UTSW 7 108937817 critical splice donor site probably null
R1886:Eif3f UTSW 7 108940751 missense probably benign 0.08
R2877:Eif3f UTSW 7 108934812 splice site probably null
R3700:Eif3f UTSW 7 108940275 missense probably benign 0.23
R3882:Eif3f UTSW 7 108940955 missense possibly damaging 0.88
R4471:Eif3f UTSW 7 108940946 missense possibly damaging 0.79
R4472:Eif3f UTSW 7 108940946 missense possibly damaging 0.79
R4817:Eif3f UTSW 7 108937775 missense probably damaging 0.99
R4953:Eif3f UTSW 7 108934640 unclassified probably benign
R5744:Eif3f UTSW 7 108938417 missense probably damaging 0.99
R7162:Eif3f UTSW 7 108940731 missense probably benign 0.02
R7445:Eif3f UTSW 7 108934658 missense unknown
Predicted Primers PCR Primer
(F):5'- CCACTGTGCTGTGTCTGTAG -3'
(R):5'- GGTTCTCACACCCATTTTAGCAG -3'

Sequencing Primer
(F):5'- TCTGTAGCCAGAGCTGC -3'
(R):5'- TCACACCCATTTTAGCAGTAATTAAC -3'
Posted On2019-06-26