Mutagenetix > Incidental Mutation

Incidental Mutation 'R7239:Olfr855'

563083

Institutional Source

Beutler Lab

Gene Symbol

Olfr855

Ensembl Gene

ENSMUSG00000043087

Gene Name

olfactory receptor 855

Synonyms

MOR148-1, GA_x6K02T2PVTD-13330461-13331399

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.103) question?

Stock #

R7239 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

19582617-19586030 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 19585191 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Phenylalanine at position 218 (Y218F)

Ref Sequence

ENSEMBL: ENSMUSP00000150218 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061693] [ENSMUST00000215587]

AlphaFold

Q7TRF8

Predicted Effect

probably damaging
Transcript: ENSMUST00000061693
AA Change: Y218F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000054790
Gene: ENSMUSG00000043087
AA Change: Y218F

Domain	Start	End	E-Value	Type
Pfam:7tm_4	33	310	1.7e-54	PFAM
Pfam:7TM_GPCR_Srsx	37	182	5.2e-8	PFAM
Pfam:7tm_1	43	292	2.5e-24	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000215587
AA Change: Y218F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

99% (76/77)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2510009E07Rik	A	G	16: 21,653,518	V144A	probably damaging	Het
4930402H24Rik	C	A	2: 130,806,788	R258L	unknown	Het
Abca5	A	T	11: 110,326,704	Y109N	possibly damaging	Het
Abcb5	T	C	12: 118,928,725	Q433R	probably benign	Het
Adamts7	G	A	9: 90,186,557		probably null	Het
Adgrv1	T	C	13: 81,476,612	D3746G	possibly damaging	Het
Arhgap29	C	T	3: 121,988,950	S159L	probably benign	Het
Arhgap5	T	A	12: 52,517,376	C377S	probably benign	Het
Atp1a3	G	A	7: 25,000,704	P77L	probably damaging	Het
B4galt1	T	C	4: 40,812,754	D257G	probably damaging	Het
Bin1	T	A	18: 32,406,171	N52K	probably damaging	Het
Catsperg2	A	G	7: 29,710,082	M562T	probably benign	Het
Ccdc33	G	A	9: 58,032,909	Q713*	probably null	Het
Cd96	A	T	16: 46,109,114	L156Q	probably damaging	Het
Clpb	A	G	7: 101,711,455	T231A	probably damaging	Het
Dnmt3a	A	T	12: 3,872,850	Q151L	probably benign	Het
Dock2	C	T	11: 34,231,677	V1629M	probably benign	Het
Edem1	G	A	6: 108,854,380	D601N	probably benign	Het
Eno2	C	T	6: 124,768,265	V20M	probably damaging	Het
Eny2	A	C	15: 44,433,854	D52A	probably benign	Het
Esp36	T	C	17: 38,417,241	R93G	possibly damaging	Het
Fam227a	A	G	15: 79,634,062		probably null	Het
Fat4	C	A	3: 38,983,840	H3880Q	possibly damaging	Het
Fbxw24	A	T	9: 109,605,530	V334E	possibly damaging	Het
Flrt1	T	C	19: 7,095,964	Q406R	probably benign	Het
Frmpd2	A	G	14: 33,552,077	N1092S	probably benign	Het
Gm904	C	A	13: 50,645,251	T82K	probably benign	Het
Gnas	G	C	2: 174,298,615	G252R	unknown	Het
Gpatch2l	A	G	12: 86,260,575		probably null	Het
Hist1h2ac	A	T	13: 23,683,610	I103N	probably damaging	Het
Ift74	T	C	4: 94,652,950	V204A	probably benign	Het
Ikzf4	A	G	10: 128,641,244	L119P	probably damaging	Het
Ing3	G	A	6: 21,952,194	E56K	probably damaging	Het
Klhl25	T	C	7: 75,866,768	I474T	probably benign	Het
Klhl5	T	C	5: 65,161,186	V556A	probably damaging	Het
Krt14	A	T	11: 100,204,255	M382K	probably benign	Het
Lmcd1	T	C	6: 112,315,784	V199A	possibly damaging	Het
Lpcat4	T	A	2: 112,242,707	F200I	possibly damaging	Het
Lrp1b	T	C	2: 41,004,713	T2282A		Het
Lrrk1	T	C	7: 66,262,155	T1886A	probably benign	Het
Meis2	T	C	2: 116,059,003		probably null	Het
Mki67	C	T	7: 135,700,176	R1043K	possibly damaging	Het
Myct1	G	T	10: 5,604,601	R156L	possibly damaging	Het
Nckap5l	G	T	15: 99,426,209	H804Q	probably damaging	Het
Ndst3	T	G	3: 123,606,906	E450D	probably damaging	Het
Nipbl	A	T	15: 8,292,135		probably null	Het
Npy	A	G	6: 49,823,607	N4D	probably benign	Het
Osgin2	T	C	4: 16,008,748	S18G	probably benign	Het
Pcdha9	T	G	18: 36,998,498	F207V	probably benign	Het
Pcdhb13	A	T	18: 37,444,644	I692F	probably damaging	Het
Pde3b	T	A	7: 114,416,149	V200E	probably damaging	Het
Ppp6r3	A	T	19: 3,493,981	L345I	probably benign	Het
Psd2	G	A	18: 35,980,419	A315T	probably damaging	Het
Rnf111	G	A	9: 70,469,373	T328I	probably damaging	Het
Rnf213	T	C	11: 119,458,788	L3825P		Het
Rtl1	C	T	12: 109,592,475	V977I	probably benign	Het
Scn1a	G	A	2: 66,277,656		probably null	Het
Slc25a2	G	T	18: 37,637,695	N260K	probably benign	Het
Sprtn	T	C	8: 124,900,244	V121A	probably damaging	Het
Ssx2ip	T	G	3: 146,428,016	L260W	probably damaging	Het
Syde1	G	A	10: 78,588,781	R406C	probably damaging	Het
Tab1	A	G	15: 80,133,171	R6G	probably benign	Het
Tenm4	G	A	7: 96,553,496	R106H	probably benign	Het
Tenm4	G	A	7: 96,735,813	V526I	possibly damaging	Het
Tfap2b	T	C	1: 19,234,180	F405L	probably damaging	Het
Trbv13-1	C	A	6: 41,116,391	T87K	probably benign	Het
Trmt44	G	T	5: 35,574,786	A87E	probably benign	Het
Ttll3	CAAAGTAA	CAAAGTAAAGTAA	6: 113,399,157		probably null	Het
Ttn	T	C	2: 76,787,290	D16279G	probably damaging	Het
Ttn	G	A	2: 76,881,328	R8290C	unknown	Het
Ugt2b37	T	A	5: 87,254,731	I14L	probably benign	Het
Vmn1r13	A	T	6: 57,210,626	M257L	probably benign	Het
Vmn1r184	C	T	7: 26,267,177	P116L	possibly damaging	Het
Zfp426	T	C	9: 20,470,591	T367A	probably benign	Het
Zfp647	A	T	15: 76,911,756	C235S	probably damaging	Het

Other mutations in Olfr855

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01020:Olfr855	APN	9	19585320	missense	possibly damaging	0.74
IGL01405:Olfr855	APN	9	19585205	missense	probably benign	0.23
IGL01775:Olfr855	APN	9	19584705	missense	probably benign	0.04
IGL01920:Olfr855	APN	9	19585022	missense	probably benign	0.01
R0501:Olfr855	UTSW	9	19584618	missense	probably damaging	1.00
R0600:Olfr855	UTSW	9	19585304	missense	possibly damaging	0.47
R0667:Olfr855	UTSW	9	19585447	missense	probably benign
R1769:Olfr855	UTSW	9	19585386	missense	probably damaging	0.98
R3117:Olfr855	UTSW	9	19584941	missense	probably damaging	0.99
R4002:Olfr855	UTSW	9	19584714	missense	probably damaging	1.00
R4003:Olfr855	UTSW	9	19584714	missense	probably damaging	1.00
R4043:Olfr855	UTSW	9	19584995	missense	probably benign	0.16
R4243:Olfr855	UTSW	9	19584558	missense	probably damaging	1.00
R4672:Olfr855	UTSW	9	19585430	missense	possibly damaging	0.74
R4673:Olfr855	UTSW	9	19585430	missense	possibly damaging	0.74
R4959:Olfr855	UTSW	9	19585208	missense	probably benign
R4973:Olfr855	UTSW	9	19585208	missense	probably benign
R5223:Olfr855	UTSW	9	19585026	missense	probably benign	0.16
R5681:Olfr855	UTSW	9	19584899	missense	probably damaging	1.00
R6005:Olfr855	UTSW	9	19584885	missense	probably benign	0.45
R6017:Olfr855	UTSW	9	19585434	missense	probably benign	0.00
R6145:Olfr855	UTSW	9	19584888	missense	probably benign	0.02
R6615:Olfr855	UTSW	9	19584989	missense	probably benign	0.05
R6771:Olfr855	UTSW	9	19585379	missense	probably benign	0.16
R6969:Olfr855	UTSW	9	19584590	missense	possibly damaging	0.77
R7313:Olfr855	UTSW	9	19584642	missense	probably damaging	1.00
R7361:Olfr855	UTSW	9	19584560	missense	probably benign	0.00
R8112:Olfr855	UTSW	9	19584724	missense	probably benign	0.44
R8470:Olfr855	UTSW	9	19584969	missense	probably damaging	0.99
R9155:Olfr855	UTSW	9	19585083	missense	probably benign	0.00
R9187:Olfr855	UTSW	9	19584654	missense	probably benign	0.03
R9422:Olfr855	UTSW	9	19584672	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAGCTATTTGTCACCCATTAAGG -3'
(R):5'- GCCACTGCAGATTTTCCTGG -3'

Sequencing Primer
(F):5'- AGTGTTATGGATTCCCTGATTCACAC -3'
(R):5'- CAGATTTTCCTGGTGAGGCAC -3'

Posted On

2019-06-26