Mutagenetix > Incidental Mutation

Incidental Mutation 'R7240:Zfp777'

563138

Institutional Source

Beutler Lab

Gene Symbol

Zfp777

Ensembl Gene

ENSMUSG00000071477

Gene Name

zinc finger protein 777

Synonyms

MMRRC Submission

Accession Numbers

Essential gene?

Probably essential (E-score: 0.856) question?

Stock #

R7240 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

48024188-48048911 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 48044449 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 80 (S80P)

Ref Sequence

ENSEMBL: ENSMUSP00000093637 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000095944] [ENSMUST00000114583] [ENSMUST00000125385] [ENSMUST00000147281]

AlphaFold

B9EKF4

Predicted Effect

probably benign
Transcript: ENSMUST00000095944
AA Change: S80P

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000093637
Gene: ENSMUSG00000071477
AA Change: S80P

Domain	Start	End	E-Value	Type
Pfam:DUF3669	177	256	4.3e-12	PFAM
KRAB	284	344	1.6e-29	SMART
low complexity region	422	433	N/A	INTRINSIC
coiled coil region	454	477	N/A	INTRINSIC
low complexity region	489	505	N/A	INTRINSIC
low complexity region	520	532	N/A	INTRINSIC
low complexity region	539	555	N/A	INTRINSIC
ZnF_C2H2	557	579	1.2e-5	SMART
ZnF_C2H2	585	607	3.8e-5	SMART
ZnF_C2H2	646	668	1.1e-6	SMART
ZnF_C2H2	674	696	4.6e-6	SMART
ZnF_C2H2	704	726	3.2e-7	SMART
ZnF_C2H2	732	754	3.3e-6	SMART
ZnF_C2H2	760	782	8.4e-6	SMART
ZnF_C2H2	788	810	4.9e-5	SMART
ZnF_C2H2	816	838	1.1e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000114583
AA Change: S124P

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000110230
Gene: ENSMUSG00000071477
AA Change: S124P

Domain	Start	End	E-Value	Type
low complexity region	2	24	N/A	INTRINSIC
Pfam:DUF3669	231	298	4.7e-12	PFAM
KRAB	328	388	3.96e-27	SMART
low complexity region	466	477	N/A	INTRINSIC
coiled coil region	498	521	N/A	INTRINSIC
low complexity region	533	549	N/A	INTRINSIC
low complexity region	564	576	N/A	INTRINSIC
low complexity region	583	599	N/A	INTRINSIC
ZnF_C2H2	601	623	2.95e-3	SMART
ZnF_C2H2	629	651	8.94e-3	SMART
ZnF_C2H2	690	712	2.43e-4	SMART
ZnF_C2H2	718	740	1.12e-3	SMART
ZnF_C2H2	748	770	7.49e-5	SMART
ZnF_C2H2	776	798	7.9e-4	SMART
ZnF_C2H2	804	826	1.95e-3	SMART
ZnF_C2H2	832	854	1.18e-2	SMART
ZnF_C2H2	860	882	2.53e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000125385
AA Change: S80P

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

Predicted Effect

probably benign
Transcript: ENSMUST00000147281
AA Change: S80P

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

Predicted Effect

probably benign
Transcript: ENSMUST00000148362

SMART Domains

Protein: ENSMUSP00000116303
Gene: ENSMUSG00000071477

Domain	Start	End	E-Value	Type
Pfam:DUF3669	40	107	6.1e-13	PFAM
KRAB	137	197	3.96e-27	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

96% (50/52)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210017I01Rik	A	G	3: 92,605,075	I59T	unknown	Het
Aspm	C	T	1: 139,478,651	Q1759*	probably null	Het
Atn1	A	T	6: 124,747,898	I124K	unknown	Het
Catsper2	TAGGATGGCTTTTCTCAGGATAGCTTTTCTCAGGATGGCTTTTCTCAGGATAGCTTTTCTCAGGATGGCTTTTCTCAGGATAGCTTTTCT	TAGGATGGCTTTTCTCAGGATAGCTTTTCTCAGGATGGCTTTTCTCAGGATAGCTTTTCT	2: 121,397,572		probably benign	Het
Ccdc88c	C	T	12: 100,944,939	V879M	probably benign	Het
Cd300c	A	G	11: 114,959,783	C65R	possibly damaging	Het
Cd69	A	T	6: 129,270,042	S112T	possibly damaging	Het
Cdh19	T	C	1: 110,893,407	T534A	probably benign	Het
Cdk5rap2	T	A	4: 70,291,908	D701V	probably damaging	Het
D130052B06Rik	A	G	11: 33,623,874	H157R	possibly damaging	Het
Dpf1	T	A	7: 29,311,627	F150L	probably benign	Het
Dsg1c	T	C	18: 20,283,109	L689P	probably damaging	Het
Dstyk	G	A	1: 132,454,123	M538I	probably benign	Het
E4f1	T	A	17: 24,444,325	I669F	probably damaging	Het
Gm7138	T	C	10: 77,776,755	T64A	unknown	Het
Gnai2	A	C	9: 107,615,773	D310E		Het
Iqca	A	G	1: 90,070,550	V567A	possibly damaging	Het
Iqgap1	A	T	7: 80,759,839	N249K	probably benign	Het
Lamc1	A	G	1: 153,234,650	V1093A	possibly damaging	Het
Mfap3	A	G	11: 57,529,756	K188E	probably damaging	Het
N4bp2	T	A	5: 65,794,545	V431D	probably damaging	Het
Notch4	A	G	17: 34,576,471	T792A	probably benign	Het
Ntn4	A	G	10: 93,745,741	H592R	probably damaging	Het
Ofcc1	A	G	13: 40,208,841	C202R	probably benign	Het
Olfr13	A	G	6: 43,174,501	K172E	probably benign	Het
Olfr418	T	C	1: 173,270,994	I273T	probably benign	Het
Olfr476	T	C	7: 107,968,188	S264P	probably benign	Het
Olfr497	A	G	7: 108,422,933	M121V	probably damaging	Het
Olfr922	A	G	9: 38,815,713	D70G	probably benign	Het
Olfr938	A	T	9: 39,078,610	M45K	probably damaging	Het
Parpbp	G	A	10: 88,124,940	T228I	probably damaging	Het
Pcdhgb8	G	A	18: 37,763,703	V609M	probably damaging	Het
Pla2g4d	T	C	2: 120,270,349	N543S	probably damaging	Het
Puf60	A	G	15: 76,072,539		probably benign	Het
Rbm6	A	T	9: 107,852,896	D184E	probably damaging	Het
Rnase9	A	C	14: 51,038,979	S181A	probably benign	Het
Rnpc3	T	A	3: 113,616,831	R270S	probably damaging	Het
Rundc1	T	C	11: 101,431,548		probably null	Het
Ryr1	T	C	7: 29,052,015	S3715G	possibly damaging	Het
Scn3a	A	T	2: 65,469,042	D1373E	possibly damaging	Het
Serpina3k	T	G	12: 104,340,602	I31S	probably benign	Het
Sipa1l2	A	G	8: 125,469,860	F712L	probably damaging	Het
Slc22a29	A	G	19: 8,161,511	V529A	probably damaging	Het
Tdrd3	A	G	14: 87,458,803	N58S	probably benign	Het
Tmc2	C	T	2: 130,234,804	T350I	possibly damaging	Het
Tpst2	T	C	5: 112,307,678	C28R	probably benign	Het
Trbv21	A	T	6: 41,202,958	K69N	probably benign	Het
Trpm2	A	G	10: 77,935,876		probably null	Het
Ttn	A	G	2: 76,848,990	V10796A	unknown	Het
Ush2a	A	G	1: 188,911,661	T4407A	possibly damaging	Het
Vmn2r95	A	G	17: 18,451,963	H726R	probably benign	Het

Other mutations in Zfp777

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01530:Zfp777	APN	6	48043984	missense	probably damaging	1.00
IGL01916:Zfp777	APN	6	48025342	missense	probably damaging	1.00
IGL01959:Zfp777	APN	6	48044341	missense	probably benign
IGL02167:Zfp777	APN	6	48044526	missense	probably damaging	0.98
IGL03150:Zfp777	APN	6	48044125	missense	probably damaging	1.00
R0238:Zfp777	UTSW	6	48024969	missense	probably damaging	0.99
R0238:Zfp777	UTSW	6	48024969	missense	probably damaging	0.99
R0372:Zfp777	UTSW	6	48044476	missense	possibly damaging	0.62
R0762:Zfp777	UTSW	6	48029360	missense	probably damaging	1.00
R1300:Zfp777	UTSW	6	48025770	missense	probably benign	0.43
R1727:Zfp777	UTSW	6	48043890	missense	probably damaging	0.99
R1906:Zfp777	UTSW	6	48042061	missense	probably damaging	0.99
R2047:Zfp777	UTSW	6	48044346	missense	probably benign
R2097:Zfp777	UTSW	6	48044242	missense	probably benign	0.08
R2211:Zfp777	UTSW	6	48043885	missense	possibly damaging	0.79
R2898:Zfp777	UTSW	6	48025660	missense	probably damaging	0.97
R3123:Zfp777	UTSW	6	48029116	unclassified	probably benign
R3832:Zfp777	UTSW	6	48044215	missense	probably benign	0.00
R4019:Zfp777	UTSW	6	48042112	missense	probably damaging	1.00
R4077:Zfp777	UTSW	6	48025522	missense	probably benign
R4471:Zfp777	UTSW	6	48042107	missense	probably damaging	1.00
R5021:Zfp777	UTSW	6	48042127	missense	probably damaging	0.99
R5030:Zfp777	UTSW	6	48037667	missense	probably damaging	0.99
R5819:Zfp777	UTSW	6	48037588	missense	probably damaging	0.99
R6544:Zfp777	UTSW	6	48044485	missense	probably damaging	0.98
R6736:Zfp777	UTSW	6	48024856	missense	probably damaging	0.99
R6971:Zfp777	UTSW	6	48024691	missense	probably damaging	1.00
R7258:Zfp777	UTSW	6	48025797	missense	probably damaging	0.99
R7586:Zfp777	UTSW	6	48029218	missense	probably benign	0.33
R7833:Zfp777	UTSW	6	48025138	missense	probably damaging	0.99
R7947:Zfp777	UTSW	6	48024711	missense	probably damaging	1.00
R8136:Zfp777	UTSW	6	48044625	missense	probably benign	0.25
R8151:Zfp777	UTSW	6	48029141	nonsense	probably null
R8348:Zfp777	UTSW	6	48029167	missense	probably damaging	0.99
R8448:Zfp777	UTSW	6	48029167	missense	probably damaging	0.99
R8942:Zfp777	UTSW	6	48029191	missense	probably benign	0.25
R8983:Zfp777	UTSW	6	48029224	missense	probably damaging	1.00
R9205:Zfp777	UTSW	6	48025587	missense	probably benign	0.07
R9397:Zfp777	UTSW	6	48044256	missense	probably benign	0.00
R9562:Zfp777	UTSW	6	48044646	missense	possibly damaging	0.84
R9565:Zfp777	UTSW	6	48044646	missense	possibly damaging	0.84
RF008:Zfp777	UTSW	6	48042048	nonsense	probably null
Z1177:Zfp777	UTSW	6	48025234	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTCTCAGGAACCTCAGGAGAG -3'
(R):5'- CTTGAACACAGGTCCAGCAG -3'

Sequencing Primer
(F):5'- AGTGGAGTGGAGCTTCCTGAC -3'
(R):5'- TGTCCCACACGAAGAGTCTGTG -3'

Posted On

2019-06-26