Mutagenetix > Incidental Mutation

Incidental Mutation 'R7250:Vcan'

563867

Institutional Source

Beutler Lab

Gene Symbol

Vcan

Ensembl Gene

ENSMUSG00000021614

Gene Name

versican

Synonyms

PG-M, hdf, heart defect, Cspg2, DPEAAE, 5430420N07Rik

MMRRC Submission

045350-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7250 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

89803431-89890628 bp(-) (GRCm39)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

A to T at 89879576 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000105173 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109543] [ENSMUST00000109544] [ENSMUST00000109546] [ENSMUST00000159337] [ENSMUST00000159910] [ENSMUST00000162715]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000109543

SMART Domains

Protein: ENSMUSP00000105170
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
EGF	351	384	2.72e-7	SMART
EGF_CA	386	422	1.16e-10	SMART
CLECT	428	549	3.08e-34	SMART
CCP	555	611	1.04e-8	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000109544

SMART Domains

Protein: ENSMUSP00000105171
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
EGF	1311	1344	2.72e-7	SMART
EGF_CA	1346	1382	1.16e-10	SMART
CLECT	1388	1509	3.08e-34	SMART
CCP	1515	1571	1.04e-8	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000109546

SMART Domains

Protein: ENSMUSP00000105173
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
low complexity region	1322	1333	N/A	INTRINSIC
low complexity region	1546	1569	N/A	INTRINSIC
low complexity region	1837	1852	N/A	INTRINSIC
low complexity region	2013	2026	N/A	INTRINSIC
low complexity region	2354	2367	N/A	INTRINSIC
low complexity region	2468	2482	N/A	INTRINSIC
low complexity region	2719	2728	N/A	INTRINSIC
EGF	3050	3083	2.72e-7	SMART
EGF_CA	3085	3121	1.16e-10	SMART
CLECT	3127	3248	3.08e-34	SMART
CCP	3254	3310	1.04e-8	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000159337

SMART Domains

Protein: ENSMUSP00000125432
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	354	365	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000159910

SMART Domains

Protein: ENSMUSP00000125446
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	362	373	N/A	INTRINSIC
low complexity region	586	609	N/A	INTRINSIC
low complexity region	877	892	N/A	INTRINSIC
low complexity region	1053	1066	N/A	INTRINSIC
low complexity region	1394	1407	N/A	INTRINSIC
low complexity region	1508	1522	N/A	INTRINSIC
low complexity region	1759	1768	N/A	INTRINSIC
EGF	2090	2123	2.72e-7	SMART
EGF_CA	2125	2161	1.16e-10	SMART
CLECT	2167	2288	3.08e-34	SMART
CCP	2294	2350	1.04e-8	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000162715

SMART Domains

Protein: ENSMUSP00000125521
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
Pfam:V-set	23	100	3.9e-6	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
PHENOTYPE: Homozygotes for an insertional mutation exhibit anterior-posterior segmental defects of the heart, lack endocardial cushions of the conus and atrioventricular region, and die and around embryonic day 10.5. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 101 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930480E11Rik	G	T	X: 77,414,311 (GRCm39)	M345I	probably benign	Het
Actl7b	T	A	4: 56,741,035 (GRCm39)	M108L	probably benign	Het
Adgrf3	T	A	5: 30,400,680 (GRCm39)	I934L	probably damaging	Het
Adprm	A	G	11: 66,932,450 (GRCm39)	V153A	probably benign	Het
Aif1l	G	A	2: 31,859,764 (GRCm39)	V109M	probably damaging	Het
Asphd1	T	A	7: 126,545,942 (GRCm39)	E307V	probably damaging	Het
Ate1	T	A	7: 130,121,701 (GRCm39)		probably benign	Het
Borcs7	A	G	19: 46,688,047 (GRCm39)	H64R	probably damaging	Het
C1qtnf1	A	G	11: 118,339,176 (GRCm39)	*282W	probably null	Het
Cacna1c	A	G	6: 118,574,966 (GRCm39)	C1985R		Het
Cacna1c	A	T	6: 118,673,412 (GRCm39)	V647E		Het
Cacna1d	A	G	14: 29,797,108 (GRCm39)	S1497P	probably damaging	Het
Cacna1e	T	A	1: 154,576,235 (GRCm39)	I133F	possibly damaging	Het
Ccdc63	A	T	5: 122,260,906 (GRCm39)	L206H	probably damaging	Het
Cenatac	A	G	9: 44,323,748 (GRCm39)		probably null	Het
Cspg4b	T	G	13: 113,455,349 (GRCm39)	I465R		Het
D430041D05Rik	T	C	2: 104,086,961 (GRCm39)	T672A	possibly damaging	Het
D630045J12Rik	G	T	6: 38,119,546 (GRCm39)	T1732K	possibly damaging	Het
D930020B18Rik	T	C	10: 121,507,736 (GRCm39)	I158T	probably damaging	Het
Ddx52	G	A	11: 83,835,392 (GRCm39)	G106D	probably benign	Het
Dock2	A	G	11: 34,586,032 (GRCm39)	V550A	probably benign	Het
Dock2	C	T	11: 34,586,120 (GRCm39)	D521N	probably damaging	Het
F13b	T	C	1: 139,444,227 (GRCm39)		probably null	Het
Fchsd2	A	G	7: 100,908,892 (GRCm39)	K431R	possibly damaging	Het
Fez1	C	T	9: 36,779,090 (GRCm39)	R256C	probably damaging	Het
Gjd4	T	A	18: 9,280,391 (GRCm39)	Q229L	probably benign	Het
Gpr45	T	A	1: 43,071,531 (GRCm39)	I58N	probably damaging	Het
Gstm1	T	A	3: 107,923,709 (GRCm39)	I99F	probably damaging	Het
Gtpbp8	T	A	16: 44,564,225 (GRCm39)	T149S	probably damaging	Het
H2-Ab1	A	G	17: 34,486,481 (GRCm39)	D180G	probably damaging	Het
Hdhd5	A	G	6: 120,494,016 (GRCm39)	I188T	possibly damaging	Het
Hfm1	T	C	5: 107,052,197 (GRCm39)	I300V	probably benign	Het
Hnrnpr	T	G	4: 136,059,746 (GRCm39)	D283E	probably benign	Het
Hsp90b1	T	C	10: 86,527,572 (GRCm39)	E768G	unknown	Het
Kcna4	C	A	2: 107,126,663 (GRCm39)	Q466K	possibly damaging	Het
Kcnk6	A	G	7: 28,931,619 (GRCm39)	L97P	probably benign	Het
Kics2	T	C	10: 121,581,376 (GRCm39)	S126P	possibly damaging	Het
Klf5	A	G	14: 99,536,455 (GRCm39)	S9G	probably benign	Het
Kmt2c	C	A	5: 25,504,489 (GRCm39)	K3606N	probably damaging	Het
Kmt2c	T	C	5: 25,514,805 (GRCm39)	T3013A	probably benign	Het
Lancl1	A	G	1: 67,048,458 (GRCm39)	Y207H	possibly damaging	Het
Lipe	G	C	7: 25,088,085 (GRCm39)		probably benign	Het
Lrrc66	T	A	5: 73,768,224 (GRCm39)	H239L	probably benign	Het
Ltbp2	C	T	12: 84,834,166 (GRCm39)	W1441*	probably null	Het
Man2a1	T	C	17: 64,943,583 (GRCm39)	S213P	probably benign	Het
Mapre2	C	T	18: 23,991,119 (GRCm39)	A171V	possibly damaging	Het
Mdn1	T	A	4: 32,695,427 (GRCm39)	D1155E	probably damaging	Het
Mki67	T	C	7: 135,301,053 (GRCm39)	D1327G	possibly damaging	Het
Mx2	T	C	16: 97,348,664 (GRCm39)	I279T	probably damaging	Het
Myo5c	A	G	9: 75,169,497 (GRCm39)	T441A	probably damaging	Het
Nlrp9a	T	G	7: 26,258,143 (GRCm39)	V587G	possibly damaging	Het
Npas2	A	T	1: 39,377,188 (GRCm39)	T517S	probably damaging	Het
Npy1r	T	C	8: 67,157,712 (GRCm39)	S341P	probably benign	Het
Ntm	A	G	9: 29,322,988 (GRCm39)	W11R	probably benign	Het
Nxpe2	A	T	9: 48,238,096 (GRCm39)	I53N	possibly damaging	Het
Onecut2	T	C	18: 64,519,511 (GRCm39)	F443L	probably benign	Het
Or10g6	T	A	9: 39,934,050 (GRCm39)	Y120*	probably null	Het
Or2r2	A	G	6: 42,463,689 (GRCm39)	V146A	probably benign	Het
Or52ac1	A	T	7: 104,245,738 (GRCm39)	Y217N	probably damaging	Het
Parp2	T	A	14: 51,054,801 (GRCm39)	V248E	probably benign	Het
Pcdh12	C	A	18: 38,415,029 (GRCm39)	V699L	probably benign	Het
Pja2	G	A	17: 64,616,451 (GRCm39)	P148L	probably benign	Het
Poglut3	C	T	9: 53,301,821 (GRCm39)	Q158*	probably null	Het
Ppip5k2	T	G	1: 97,673,187 (GRCm39)	D415A	probably benign	Het
Prss47	A	G	13: 65,200,355 (GRCm39)	S74P	probably benign	Het
Ptprg	G	T	14: 12,166,767 (GRCm38)	M723I	probably benign	Het
Qsox2	A	T	2: 26,118,444 (GRCm39)	I109N	probably damaging	Het
Ranbp3l	A	G	15: 9,041,853 (GRCm39)	E217G	probably benign	Het
Rgl2	C	T	17: 34,152,403 (GRCm39)	R367W	probably damaging	Het
Rgs12	T	A	5: 35,122,841 (GRCm39)	F208Y	probably damaging	Het
Rin3	C	T	12: 102,334,893 (GRCm39)	T268I	unknown	Het
Rttn	T	A	18: 89,007,647 (GRCm39)	D427E	probably benign	Het
Samd13	G	A	3: 146,352,079 (GRCm39)	P91S	probably benign	Het
Samd9l	T	C	6: 3,374,201 (GRCm39)	D1020G	possibly damaging	Het
Sec62	T	G	3: 30,866,496 (GRCm39)	L201V	possibly damaging	Het
Setdb1	C	T	3: 95,261,852 (GRCm39)		probably null	Het
Sf3a3	A	T	4: 124,616,708 (GRCm39)	T197S	probably benign	Het
Slc20a1	A	T	2: 129,051,844 (GRCm39)	I618F	possibly damaging	Het
Slc38a3	A	T	9: 107,533,865 (GRCm39)	M186K	probably benign	Het
Slc39a13	T	C	2: 90,893,503 (GRCm39)	T319A	probably benign	Het
Slc44a4	T	C	17: 35,137,520 (GRCm39)		probably null	Het
St3gal1	A	C	15: 66,978,578 (GRCm39)	D314E	possibly damaging	Het
Suclg1	A	G	6: 73,248,074 (GRCm39)	N265S	probably benign	Het
Sult2b1	A	T	7: 45,433,361 (GRCm39)	V2D	unknown	Het
Supv3l1	T	A	10: 62,280,846 (GRCm39)	I182F	probably damaging	Het
Tcp11l2	T	C	10: 84,423,105 (GRCm39)		probably null	Het
Tenm2	C	A	11: 35,963,625 (GRCm39)	L935F	probably damaging	Het
Tnks	T	A	8: 35,318,912 (GRCm39)	I790F	probably damaging	Het
Top2b	C	T	14: 16,420,411 (GRCm38)	T1274I	probably benign	Het
Tpi1	A	T	6: 124,789,441 (GRCm39)	I178N	probably damaging	Het
Trav9d-1	A	T	14: 53,030,153 (GRCm39)	S86C	probably damaging	Het
Treml2	A	G	17: 48,616,155 (GRCm39)	E265G	probably benign	Het
Trpm7	A	T	2: 126,668,685 (GRCm39)	S744T	possibly damaging	Het
Usp33	T	A	3: 152,097,999 (GRCm39)	L909*	probably null	Het
Vsig10l	A	T	7: 43,113,099 (GRCm39)	D17V	probably benign	Het
Zbtb7b	T	A	3: 89,286,976 (GRCm39)	T498S	probably benign	Het
Zfp366	T	A	13: 99,366,076 (GRCm39)	H412Q	probably damaging	Het
Zfp53	C	A	17: 21,729,840 (GRCm39)	D624E	probably damaging	Het
Zfp827	A	T	8: 79,916,721 (GRCm39)	D432V		Het
Zic1	T	C	9: 91,247,028 (GRCm39)	T15A	probably damaging	Het
Zswim8	T	C	14: 20,770,036 (GRCm39)	C1368R	probably damaging	Het

Other mutations in Vcan

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00434:Vcan	APN	13	89,852,821 (GRCm39)	missense	probably damaging	1.00
IGL00502:Vcan	APN	13	89,840,438 (GRCm39)	missense	probably benign
IGL00504:Vcan	APN	13	89,839,394 (GRCm39)	missense	possibly damaging	0.70
IGL00566:Vcan	APN	13	89,837,098 (GRCm39)	missense	probably benign	0.01
IGL00701:Vcan	APN	13	89,851,845 (GRCm39)	missense	probably benign
IGL00743:Vcan	APN	13	89,873,425 (GRCm39)	missense	probably damaging	0.98
IGL00962:Vcan	APN	13	89,810,171 (GRCm39)	missense	probably damaging	1.00
IGL01085:Vcan	APN	13	89,828,077 (GRCm39)	missense	probably damaging	1.00
IGL01317:Vcan	APN	13	89,839,787 (GRCm39)	missense	probably benign	0.00
IGL01349:Vcan	APN	13	89,852,062 (GRCm39)	missense	probably damaging	0.98
IGL01391:Vcan	APN	13	89,852,288 (GRCm39)	missense	probably benign	0.19
IGL01644:Vcan	APN	13	89,836,794 (GRCm39)	missense	probably benign	0.13
IGL01657:Vcan	APN	13	89,838,705 (GRCm39)	missense	probably damaging	1.00
IGL01707:Vcan	APN	13	89,837,864 (GRCm39)	missense	probably damaging	1.00
IGL01764:Vcan	APN	13	89,873,507 (GRCm39)	missense	probably damaging	1.00
IGL01920:Vcan	APN	13	89,837,324 (GRCm39)	missense	probably benign	0.04
IGL01989:Vcan	APN	13	89,837,478 (GRCm39)	missense	possibly damaging	0.86
IGL01999:Vcan	APN	13	89,832,557 (GRCm39)	missense	probably damaging	1.00
IGL02083:Vcan	APN	13	89,873,684 (GRCm39)	missense	probably damaging	1.00
IGL02160:Vcan	APN	13	89,832,612 (GRCm39)	missense	probably damaging	1.00
IGL02217:Vcan	APN	13	89,851,196 (GRCm39)	missense	probably damaging	1.00
IGL02522:Vcan	APN	13	89,852,968 (GRCm39)	missense	probably benign	0.00
IGL02527:Vcan	APN	13	89,838,776 (GRCm39)	missense	possibly damaging	0.95
IGL02926:Vcan	APN	13	89,836,742 (GRCm39)	missense	probably damaging	0.98
IGL03061:Vcan	APN	13	89,851,394 (GRCm39)	missense	probably benign	0.25
IGL03331:Vcan	APN	13	89,810,051 (GRCm39)	missense	probably damaging	1.00
IGL03352:Vcan	APN	13	89,853,125 (GRCm39)	missense	probably benign	0.00
R0041:Vcan	UTSW	13	89,810,104 (GRCm39)	missense	probably damaging	1.00
R0102:Vcan	UTSW	13	89,851,787 (GRCm39)	missense	probably benign	0.01
R0102:Vcan	UTSW	13	89,851,787 (GRCm39)	missense	probably benign	0.01
R0109:Vcan	UTSW	13	89,826,192 (GRCm39)	critical splice donor site	probably null
R0139:Vcan	UTSW	13	89,839,380 (GRCm39)	missense	probably damaging	1.00
R0295:Vcan	UTSW	13	89,860,310 (GRCm39)	missense	probably benign	0.06
R0375:Vcan	UTSW	13	89,839,394 (GRCm39)	missense	probably damaging	0.99
R0379:Vcan	UTSW	13	89,851,665 (GRCm39)	missense	probably damaging	0.99
R0457:Vcan	UTSW	13	89,851,318 (GRCm39)	missense	possibly damaging	0.78
R0482:Vcan	UTSW	13	89,826,264 (GRCm39)	missense	probably damaging	1.00
R0485:Vcan	UTSW	13	89,852,779 (GRCm39)	missense	possibly damaging	0.92
R0532:Vcan	UTSW	13	89,851,891 (GRCm39)	missense	probably damaging	0.99
R0561:Vcan	UTSW	13	89,879,583 (GRCm39)	missense	possibly damaging	0.86
R0561:Vcan	UTSW	13	89,860,372 (GRCm39)	missense	probably damaging	1.00
R0636:Vcan	UTSW	13	89,860,386 (GRCm39)	missense	probably damaging	1.00
R0636:Vcan	UTSW	13	89,852,825 (GRCm39)	missense	probably damaging	0.99
R0680:Vcan	UTSW	13	89,827,941 (GRCm39)	missense	probably damaging	1.00
R0849:Vcan	UTSW	13	89,853,072 (GRCm39)	missense	possibly damaging	0.75
R1006:Vcan	UTSW	13	89,833,196 (GRCm39)	critical splice donor site	probably null
R1104:Vcan	UTSW	13	89,840,529 (GRCm39)	missense	probably damaging	1.00
R1118:Vcan	UTSW	13	89,853,782 (GRCm39)	missense	probably damaging	1.00
R1137:Vcan	UTSW	13	89,852,422 (GRCm39)	missense	probably damaging	1.00
R1199:Vcan	UTSW	13	89,827,913 (GRCm39)	splice site	probably null
R1219:Vcan	UTSW	13	89,828,023 (GRCm39)	missense	probably damaging	1.00
R1296:Vcan	UTSW	13	89,805,675 (GRCm39)	missense	probably damaging	1.00
R1332:Vcan	UTSW	13	89,841,174 (GRCm39)	missense	probably damaging	1.00
R1336:Vcan	UTSW	13	89,841,174 (GRCm39)	missense	probably damaging	1.00
R1403:Vcan	UTSW	13	89,836,603 (GRCm39)	missense	probably benign	0.00
R1403:Vcan	UTSW	13	89,836,603 (GRCm39)	missense	probably benign	0.00
R1546:Vcan	UTSW	13	89,841,075 (GRCm39)	missense	probably damaging	0.99
R1604:Vcan	UTSW	13	89,837,780 (GRCm39)	missense	probably benign	0.42
R1616:Vcan	UTSW	13	89,853,782 (GRCm39)	missense	probably damaging	1.00
R1636:Vcan	UTSW	13	89,851,786 (GRCm39)	missense	possibly damaging	0.90
R1654:Vcan	UTSW	13	89,810,065 (GRCm39)	missense	probably damaging	1.00
R1680:Vcan	UTSW	13	89,851,666 (GRCm39)	missense	probably benign	0.19
R1694:Vcan	UTSW	13	89,836,602 (GRCm39)	missense	probably damaging	0.98
R1712:Vcan	UTSW	13	89,869,894 (GRCm39)	missense	probably damaging	1.00
R1754:Vcan	UTSW	13	89,852,854 (GRCm39)	missense	probably benign	0.01
R1756:Vcan	UTSW	13	89,839,800 (GRCm39)	missense	probably benign	0.05
R1824:Vcan	UTSW	13	89,853,331 (GRCm39)	missense	possibly damaging	0.75
R1852:Vcan	UTSW	13	89,853,511 (GRCm39)	missense	probably damaging	0.99
R1868:Vcan	UTSW	13	89,838,990 (GRCm39)	missense	probably benign	0.12
R1920:Vcan	UTSW	13	89,841,134 (GRCm39)	missense	probably damaging	1.00
R1932:Vcan	UTSW	13	89,853,653 (GRCm39)	missense	possibly damaging	0.78
R1934:Vcan	UTSW	13	89,851,045 (GRCm39)	missense	probably damaging	1.00
R1942:Vcan	UTSW	13	89,851,543 (GRCm39)	missense	probably benign	0.01
R1964:Vcan	UTSW	13	89,840,861 (GRCm39)	missense	probably benign	0.02
R1970:Vcan	UTSW	13	89,837,157 (GRCm39)	missense	probably damaging	1.00
R2045:Vcan	UTSW	13	89,839,104 (GRCm39)	missense	probably benign	0.00
R2110:Vcan	UTSW	13	89,841,422 (GRCm39)	missense	probably damaging	1.00
R2111:Vcan	UTSW	13	89,841,422 (GRCm39)	missense	probably damaging	1.00
R2112:Vcan	UTSW	13	89,841,422 (GRCm39)	missense	probably damaging	1.00
R2136:Vcan	UTSW	13	89,837,856 (GRCm39)	missense	probably damaging	1.00
R2158:Vcan	UTSW	13	89,851,648 (GRCm39)	missense	possibly damaging	0.68
R2376:Vcan	UTSW	13	89,851,529 (GRCm39)	missense	possibly damaging	0.80
R2385:Vcan	UTSW	13	89,837,568 (GRCm39)	missense	probably damaging	1.00
R2443:Vcan	UTSW	13	89,852,794 (GRCm39)	missense	probably damaging	1.00
R2876:Vcan	UTSW	13	89,852,356 (GRCm39)	missense	probably damaging	1.00
R3607:Vcan	UTSW	13	89,851,420 (GRCm39)	missense	probably damaging	0.98
R4042:Vcan	UTSW	13	89,840,662 (GRCm39)	missense	probably benign	0.35
R4043:Vcan	UTSW	13	89,840,662 (GRCm39)	missense	probably benign	0.35
R4044:Vcan	UTSW	13	89,840,662 (GRCm39)	missense	probably benign	0.35
R4065:Vcan	UTSW	13	89,828,006 (GRCm39)	missense	probably damaging	1.00
R4161:Vcan	UTSW	13	89,833,277 (GRCm39)	missense	probably damaging	1.00
R4178:Vcan	UTSW	13	89,873,666 (GRCm39)	missense	probably damaging	1.00
R4290:Vcan	UTSW	13	89,873,605 (GRCm39)	missense	probably damaging	1.00
R4530:Vcan	UTSW	13	89,852,147 (GRCm39)	missense	probably damaging	0.97
R4666:Vcan	UTSW	13	89,828,053 (GRCm39)	missense	probably damaging	1.00
R4785:Vcan	UTSW	13	89,853,908 (GRCm39)	missense	probably damaging	1.00
R4870:Vcan	UTSW	13	89,852,858 (GRCm39)	missense	probably benign	0.01
R4973:Vcan	UTSW	13	89,836,961 (GRCm39)	missense	probably benign	0.30
R5037:Vcan	UTSW	13	89,852,096 (GRCm39)	missense	probably damaging	1.00
R5104:Vcan	UTSW	13	89,805,591 (GRCm39)	intron	probably benign
R5124:Vcan	UTSW	13	89,873,636 (GRCm39)	missense	probably damaging	1.00
R5129:Vcan	UTSW	13	89,838,359 (GRCm39)	missense	probably damaging	1.00
R5198:Vcan	UTSW	13	89,838,991 (GRCm39)	missense	probably damaging	1.00
R5240:Vcan	UTSW	13	89,840,651 (GRCm39)	missense	probably benign	0.08
R5254:Vcan	UTSW	13	89,839,719 (GRCm39)	missense	probably damaging	0.99
R5280:Vcan	UTSW	13	89,838,405 (GRCm39)	missense	probably benign	0.00
R5522:Vcan	UTSW	13	89,839,929 (GRCm39)	missense	possibly damaging	0.62
R5557:Vcan	UTSW	13	89,851,231 (GRCm39)	missense	possibly damaging	0.77
R5568:Vcan	UTSW	13	89,836,790 (GRCm39)	missense	probably damaging	1.00
R5578:Vcan	UTSW	13	89,839,622 (GRCm39)	missense	probably benign	0.01
R5627:Vcan	UTSW	13	89,839,254 (GRCm39)	frame shift	probably null
R5687:Vcan	UTSW	13	89,826,253 (GRCm39)	missense	probably damaging	1.00
R5752:Vcan	UTSW	13	89,828,069 (GRCm39)	missense	probably damaging	1.00
R5879:Vcan	UTSW	13	89,852,071 (GRCm39)	missense	probably damaging	0.99
R5941:Vcan	UTSW	13	89,840,810 (GRCm39)	missense	probably damaging	0.98
R6113:Vcan	UTSW	13	89,805,655 (GRCm39)	nonsense	probably null
R6135:Vcan	UTSW	13	89,838,045 (GRCm39)	missense	probably benign	0.36
R6252:Vcan	UTSW	13	89,839,339 (GRCm39)	nonsense	probably null
R6280:Vcan	UTSW	13	89,873,492 (GRCm39)	missense	probably damaging	1.00
R6317:Vcan	UTSW	13	89,839,716 (GRCm39)	missense	probably benign	0.22
R6327:Vcan	UTSW	13	89,852,951 (GRCm39)	missense	probably damaging	0.99
R6460:Vcan	UTSW	13	89,838,806 (GRCm39)	missense	possibly damaging	0.61
R6669:Vcan	UTSW	13	89,852,850 (GRCm39)	missense	probably benign	0.21
R6744:Vcan	UTSW	13	89,853,301 (GRCm39)	missense	probably damaging	1.00
R6819:Vcan	UTSW	13	89,853,244 (GRCm39)	missense	probably benign	0.00
R6880:Vcan	UTSW	13	89,860,500 (GRCm39)	missense	probably damaging	1.00
R6956:Vcan	UTSW	13	89,837,550 (GRCm39)	missense	probably damaging	0.99
R6971:Vcan	UTSW	13	89,826,252 (GRCm39)	missense	probably damaging	1.00
R6985:Vcan	UTSW	13	89,828,075 (GRCm39)	missense	probably damaging	1.00
R6994:Vcan	UTSW	13	89,841,526 (GRCm39)	missense	possibly damaging	0.94
R6997:Vcan	UTSW	13	89,838,737 (GRCm39)	missense	probably damaging	0.98
R7029:Vcan	UTSW	13	89,838,360 (GRCm39)	missense	probably damaging	1.00
R7066:Vcan	UTSW	13	89,853,805 (GRCm39)	missense	probably damaging	1.00
R7156:Vcan	UTSW	13	89,837,229 (GRCm39)	missense	possibly damaging	0.95
R7171:Vcan	UTSW	13	89,873,710 (GRCm39)	missense	probably damaging	1.00
R7176:Vcan	UTSW	13	89,837,055 (GRCm39)	missense	probably benign	0.01
R7229:Vcan	UTSW	13	89,853,389 (GRCm39)	missense	possibly damaging	0.87
R7250:Vcan	UTSW	13	89,869,805 (GRCm39)	missense	probably damaging	1.00
R7262:Vcan	UTSW	13	89,853,280 (GRCm39)	missense	possibly damaging	0.62
R7289:Vcan	UTSW	13	89,840,852 (GRCm39)	nonsense	probably null
R7299:Vcan	UTSW	13	89,853,385 (GRCm39)	missense	probably benign
R7301:Vcan	UTSW	13	89,853,385 (GRCm39)	missense	probably benign
R7425:Vcan	UTSW	13	89,837,951 (GRCm39)	missense	probably damaging	0.99
R7514:Vcan	UTSW	13	89,852,237 (GRCm39)	missense	probably damaging	0.97
R7579:Vcan	UTSW	13	89,840,577 (GRCm39)	missense	probably damaging	1.00
R7618:Vcan	UTSW	13	89,840,342 (GRCm39)	missense	probably damaging	0.99
R7655:Vcan	UTSW	13	89,833,233 (GRCm39)	missense	probably damaging	1.00
R7656:Vcan	UTSW	13	89,833,233 (GRCm39)	missense	probably damaging	1.00
R7676:Vcan	UTSW	13	89,839,908 (GRCm39)	missense	probably damaging	1.00
R7719:Vcan	UTSW	13	89,852,738 (GRCm39)	missense	probably damaging	0.98
R7753:Vcan	UTSW	13	89,837,442 (GRCm39)	missense	probably damaging	1.00
R7762:Vcan	UTSW	13	89,841,056 (GRCm39)	missense	probably damaging	1.00
R7778:Vcan	UTSW	13	89,836,773 (GRCm39)	missense	probably damaging	1.00
R7824:Vcan	UTSW	13	89,836,773 (GRCm39)	missense	probably damaging	1.00
R7995:Vcan	UTSW	13	89,839,977 (GRCm39)	missense	probably benign
R7998:Vcan	UTSW	13	89,852,446 (GRCm39)	missense	probably damaging	1.00
R8033:Vcan	UTSW	13	89,852,479 (GRCm39)	missense	probably benign	0.04
R8061:Vcan	UTSW	13	89,805,409 (GRCm39)	missense	probably benign	0.45
R8103:Vcan	UTSW	13	89,851,439 (GRCm39)	nonsense	probably null
R8103:Vcan	UTSW	13	89,805,777 (GRCm39)	missense	probably damaging	1.00
R8124:Vcan	UTSW	13	89,852,373 (GRCm39)	missense	possibly damaging	0.93
R8162:Vcan	UTSW	13	89,853,106 (GRCm39)	nonsense	probably null
R8166:Vcan	UTSW	13	89,840,855 (GRCm39)	missense	probably benign	0.02
R8274:Vcan	UTSW	13	89,853,089 (GRCm39)	missense	probably benign	0.02
R8284:Vcan	UTSW	13	89,852,454 (GRCm39)	missense	possibly damaging	0.68
R8417:Vcan	UTSW	13	89,836,862 (GRCm39)	missense	probably benign	0.19
R8696:Vcan	UTSW	13	89,839,217 (GRCm39)	missense	probably benign	0.00
R8738:Vcan	UTSW	13	89,840,439 (GRCm39)	missense	probably benign	0.17
R8792:Vcan	UTSW	13	89,840,230 (GRCm39)	missense	possibly damaging	0.91
R8887:Vcan	UTSW	13	89,853,026 (GRCm39)	missense	probably benign
R9049:Vcan	UTSW	13	89,826,224 (GRCm39)	missense	probably damaging	1.00
R9074:Vcan	UTSW	13	89,839,146 (GRCm39)	missense	possibly damaging	0.95
R9095:Vcan	UTSW	13	89,852,644 (GRCm39)	missense	probably benign	0.32
R9172:Vcan	UTSW	13	89,828,050 (GRCm39)	missense	probably damaging	1.00
R9199:Vcan	UTSW	13	89,838,615 (GRCm39)	nonsense	probably null
R9259:Vcan	UTSW	13	89,838,989 (GRCm39)	missense	probably damaging	0.99
R9455:Vcan	UTSW	13	89,837,452 (GRCm39)	missense	probably damaging	1.00
R9476:Vcan	UTSW	13	89,851,531 (GRCm39)	missense	possibly damaging	0.95
R9477:Vcan	UTSW	13	89,841,128 (GRCm39)	missense	probably damaging	1.00
R9555:Vcan	UTSW	13	89,839,659 (GRCm39)	missense
R9579:Vcan	UTSW	13	89,837,713 (GRCm39)	missense	possibly damaging	0.67
R9606:Vcan	UTSW	13	89,853,491 (GRCm39)	missense	probably damaging	1.00
R9645:Vcan	UTSW	13	89,841,081 (GRCm39)	missense	probably benign	0.00
R9659:Vcan	UTSW	13	89,839,860 (GRCm39)	missense	probably damaging	0.99
R9766:Vcan	UTSW	13	89,839,247 (GRCm39)	missense	probably benign	0.00
R9778:Vcan	UTSW	13	89,837,930 (GRCm39)	missense	probably damaging	1.00
X0058:Vcan	UTSW	13	89,840,612 (GRCm39)	missense	probably benign	0.21
X0065:Vcan	UTSW	13	89,853,868 (GRCm39)	missense	probably damaging	0.96
Z1176:Vcan	UTSW	13	89,840,690 (GRCm39)	missense	probably benign	0.10
Z1177:Vcan	UTSW	13	89,852,192 (GRCm39)	missense	probably damaging	1.00
Z1177:Vcan	UTSW	13	89,851,907 (GRCm39)	nonsense	probably null
Z1177:Vcan	UTSW	13	89,851,643 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GTTGCACAGTTCAACTTGAGG -3'
(R):5'- GTGTTTACAACCAGCAGATGAC -3'

Sequencing Primer
(F):5'- CTCATGAGTTGAGCCTACCTAGAG -3'
(R):5'- GTACTCGGTTCCTTATTAACG -3'

Posted On

2019-06-26