Incidental Mutation 'R7251:Nek10'
| ID |
563947 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Nek10
|
| Ensembl Gene |
ENSMUSG00000042567 |
| Gene Name |
NIMA (never in mitosis gene a)- related kinase 10 |
| Synonyms |
LOC238944 |
| MMRRC Submission |
045313-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R7251 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
14 |
| Chromosomal Location |
7457704-7666183 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 14853965 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Threonine to Alanine
at position 384
(T384A)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000108249
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000112630]
[ENSMUST00000112631]
[ENSMUST00000224491]
|
| AlphaFold |
Q3UGM2 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000112630
AA Change: T384A
PolyPhen 2
Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
|
| SMART Domains |
Protein: ENSMUSP00000108249
Gene: ENSMUSG00000042567
AA Change: T384A
| Domain | Start | End | E-Value | Type |
|---|
|
ARM
|
197 |
238 |
8.23e1 |
SMART |
|
ARM
|
278 |
320 |
5.18e0 |
SMART |
|
low complexity region
|
387 |
400 |
N/A |
INTRINSIC |
|
ARM
|
401 |
448 |
7.09e1 |
SMART |
|
S_TKc
|
519 |
791 |
2.36e-75 |
SMART |
|
low complexity region
|
799 |
811 |
N/A |
INTRINSIC |
|
low complexity region
|
839 |
863 |
N/A |
INTRINSIC |
|
low complexity region
|
908 |
926 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000112631
AA Change: T384A
PolyPhen 2
Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
|
| SMART Domains |
Protein: ENSMUSP00000108250
Gene: ENSMUSG00000042567
AA Change: T384A
| Domain | Start | End | E-Value | Type |
|---|
|
ARM
|
197 |
238 |
8.23e1 |
SMART |
|
ARM
|
278 |
320 |
5.18e0 |
SMART |
|
low complexity region
|
387 |
400 |
N/A |
INTRINSIC |
|
ARM
|
401 |
448 |
7.09e1 |
SMART |
|
S_TKc
|
519 |
791 |
2.36e-75 |
SMART |
|
low complexity region
|
799 |
811 |
N/A |
INTRINSIC |
|
low complexity region
|
839 |
863 |
N/A |
INTRINSIC |
|
low complexity region
|
908 |
926 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000224491
AA Change: T384A
PolyPhen 2
Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
|
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 99.9%
- 10x: 99.7%
- 20x: 98.9%
|
| Validation Efficiency |
97% (66/68) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 67 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4930480E11Rik |
G |
T |
X: 77,414,311 (GRCm39) |
M345I |
probably benign |
Het |
| Adamts19 |
A |
T |
18: 58,970,974 (GRCm39) |
D186V |
probably damaging |
Het |
| Agrn |
T |
C |
4: 156,259,063 (GRCm39) |
D884G |
probably damaging |
Het |
| Akr1c6 |
T |
A |
13: 4,497,019 (GRCm39) |
C154S |
probably damaging |
Het |
| Apob |
T |
C |
12: 8,057,037 (GRCm39) |
Y1840H |
probably damaging |
Het |
| Arfgef1 |
T |
C |
1: 10,269,200 (GRCm39) |
E399G |
possibly damaging |
Het |
| Arg2 |
G |
A |
12: 79,197,572 (GRCm39) |
G197S |
probably damaging |
Het |
| Arhgap32 |
T |
C |
9: 32,119,481 (GRCm39) |
V308A |
probably damaging |
Het |
| Atcay |
A |
T |
10: 81,046,366 (GRCm39) |
C319* |
probably null |
Het |
| Bend4 |
C |
G |
5: 67,584,876 (GRCm39) |
R16P |
unknown |
Het |
| Braf |
A |
G |
6: 39,654,504 (GRCm39) |
|
probably null |
Het |
| Camsap1 |
T |
C |
2: 25,828,898 (GRCm39) |
E942G |
probably damaging |
Het |
| Cdpf1 |
C |
T |
15: 85,693,494 (GRCm39) |
G11D |
probably damaging |
Het |
| Cgn |
T |
C |
3: 94,683,509 (GRCm39) |
E382G |
possibly damaging |
Het |
| Cndp1 |
T |
A |
18: 84,640,322 (GRCm39) |
E294D |
probably benign |
Het |
| Cnn1 |
T |
A |
9: 22,019,513 (GRCm39) |
Y294N |
unknown |
Het |
| Cyp2ab1 |
A |
G |
16: 20,134,646 (GRCm39) |
F104S |
possibly damaging |
Het |
| Cyp2t4 |
G |
A |
7: 26,857,144 (GRCm39) |
V336M |
possibly damaging |
Het |
| D430041D05Rik |
T |
A |
2: 104,051,511 (GRCm39) |
D782V |
probably damaging |
Het |
| Ddx39b |
A |
G |
17: 35,472,464 (GRCm39) |
*429W |
probably null |
Het |
| Dgkb |
T |
C |
12: 38,031,985 (GRCm39) |
S16P |
possibly damaging |
Het |
| Dll4 |
T |
C |
2: 119,162,773 (GRCm39) |
C465R |
probably damaging |
Het |
| Dsp |
A |
G |
13: 38,377,524 (GRCm39) |
I1770V |
probably benign |
Het |
| Fbxw11 |
A |
G |
11: 32,681,370 (GRCm39) |
N250S |
probably benign |
Het |
| Fsip2 |
G |
A |
2: 82,809,425 (GRCm39) |
V1915M |
possibly damaging |
Het |
| Greb1l |
G |
A |
18: 10,515,319 (GRCm39) |
V704M |
probably damaging |
Het |
| Hgf |
T |
A |
5: 16,798,942 (GRCm39) |
N323K |
possibly damaging |
Het |
| Hhatl |
A |
T |
9: 121,614,116 (GRCm39) |
|
probably null |
Het |
| Hip1r |
T |
C |
5: 124,132,813 (GRCm39) |
S204P |
probably damaging |
Het |
| Igsf10 |
T |
A |
3: 59,226,875 (GRCm39) |
N2266I |
probably damaging |
Het |
| Krtap15-1 |
A |
G |
16: 88,625,982 (GRCm39) |
|
probably null |
Het |
| Lrp1 |
T |
C |
10: 127,408,423 (GRCm39) |
D1751G |
probably damaging |
Het |
| Madd |
A |
T |
2: 90,992,521 (GRCm39) |
D1050E |
probably benign |
Het |
| Man1c1 |
C |
T |
4: 134,308,147 (GRCm39) |
G323R |
probably damaging |
Het |
| Mgll |
A |
G |
6: 88,800,357 (GRCm39) |
E252G |
probably benign |
Het |
| Muc2 |
A |
G |
7: 141,278,965 (GRCm39) |
N145S |
possibly damaging |
Het |
| Ncoa2 |
C |
T |
1: 13,218,599 (GRCm39) |
S1410N |
probably benign |
Het |
| Nexn |
T |
C |
3: 151,952,832 (GRCm39) |
E310G |
probably damaging |
Het |
| Nol10 |
T |
C |
12: 17,452,108 (GRCm39) |
L354P |
probably damaging |
Het |
| Npy4r |
T |
C |
14: 33,868,872 (GRCm39) |
R139G |
probably damaging |
Het |
| Nup160 |
A |
G |
2: 90,530,518 (GRCm39) |
E463G |
probably damaging |
Het |
| Or14c44 |
T |
C |
7: 86,062,209 (GRCm39) |
V213A |
probably benign |
Het |
| Or52a33 |
C |
T |
7: 103,288,909 (GRCm39) |
G146D |
probably damaging |
Het |
| Or8j3c |
G |
A |
2: 86,253,940 (GRCm39) |
L27F |
probably benign |
Het |
| Pdzd8 |
A |
T |
19: 59,289,077 (GRCm39) |
N774K |
possibly damaging |
Het |
| Pmpca |
G |
C |
2: 26,285,046 (GRCm39) |
E424Q |
possibly damaging |
Het |
| Pot1a |
T |
C |
6: 25,752,497 (GRCm39) |
|
probably null |
Het |
| Ppip5k1 |
T |
A |
2: 121,178,052 (GRCm39) |
E283D |
probably benign |
Het |
| Ptpn2 |
A |
C |
18: 67,808,862 (GRCm39) |
I318R |
possibly damaging |
Het |
| Raph1 |
A |
G |
1: 60,529,027 (GRCm39) |
F745L |
unknown |
Het |
| Rgs19 |
C |
T |
2: 181,331,541 (GRCm39) |
V88I |
probably benign |
Het |
| Ripk4 |
A |
G |
16: 97,544,449 (GRCm39) |
S733P |
probably benign |
Het |
| Rprd1b |
T |
A |
2: 157,870,899 (GRCm39) |
W29R |
probably damaging |
Het |
| Rtkn |
A |
G |
6: 83,112,943 (GRCm39) |
N5S |
probably damaging |
Het |
| Sh3bp5l |
A |
T |
11: 58,232,128 (GRCm39) |
Q131L |
probably damaging |
Het |
| Slain2 |
T |
A |
5: 73,131,891 (GRCm39) |
F461I |
possibly damaging |
Het |
| Stk24 |
A |
T |
14: 121,545,434 (GRCm39) |
L108Q |
probably damaging |
Het |
| Syne3 |
TC |
T |
12: 104,927,830 (GRCm39) |
|
probably null |
Het |
| Tapbpl |
A |
T |
6: 125,203,558 (GRCm39) |
V374E |
probably damaging |
Het |
| Tax1bp1 |
A |
G |
6: 52,698,341 (GRCm39) |
I18V |
possibly damaging |
Het |
| Tecta |
T |
A |
9: 42,299,048 (GRCm39) |
I233F |
probably damaging |
Het |
| Tet3 |
A |
G |
6: 83,381,038 (GRCm39) |
S377P |
probably benign |
Het |
| Uty |
A |
G |
Y: 1,154,262 (GRCm39) |
S721P |
probably benign |
Het |
| Wnk4 |
C |
A |
11: 101,155,979 (GRCm39) |
T412K |
possibly damaging |
Het |
| Zdhhc11 |
G |
T |
13: 74,140,216 (GRCm39) |
V336L |
probably benign |
Het |
| Zfp605 |
T |
A |
5: 110,275,826 (GRCm39) |
S315T |
probably damaging |
Het |
| Zfp746 |
A |
G |
6: 48,041,811 (GRCm39) |
L305P |
probably damaging |
Het |
|
| Other mutations in Nek10 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01361:Nek10
|
APN |
14 |
14,850,957 (GRCm38) |
missense |
probably damaging |
0.99 |
|
IGL02067:Nek10
|
APN |
14 |
14,861,639 (GRCm38) |
missense |
probably benign |
0.12 |
|
IGL02361:Nek10
|
APN |
14 |
14,843,856 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL02687:Nek10
|
APN |
14 |
14,840,570 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL02929:Nek10
|
APN |
14 |
14,821,119 (GRCm38) |
missense |
possibly damaging |
0.82 |
|
IGL03229:Nek10
|
APN |
14 |
14,986,686 (GRCm38) |
missense |
probably benign |
0.10 |
|
P0041:Nek10
|
UTSW |
14 |
14,861,603 (GRCm38) |
missense |
probably benign |
0.01 |
|
R0007:Nek10
|
UTSW |
14 |
14,840,574 (GRCm38) |
missense |
probably benign |
0.10 |
|
R0007:Nek10
|
UTSW |
14 |
14,840,574 (GRCm38) |
missense |
probably benign |
0.10 |
|
R0142:Nek10
|
UTSW |
14 |
14,861,560 (GRCm38) |
missense |
possibly damaging |
0.96 |
|
R0433:Nek10
|
UTSW |
14 |
14,860,927 (GRCm38) |
missense |
probably benign |
0.32 |
|
R0633:Nek10
|
UTSW |
14 |
14,857,782 (GRCm38) |
critical splice acceptor site |
probably null |
|
|
R1087:Nek10
|
UTSW |
14 |
14,827,059 (GRCm38) |
missense |
possibly damaging |
0.59 |
|
R1184:Nek10
|
UTSW |
14 |
14,931,325 (GRCm38) |
splice site |
probably benign |
|
|
R1250:Nek10
|
UTSW |
14 |
14,853,887 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R1371:Nek10
|
UTSW |
14 |
14,850,983 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R1506:Nek10
|
UTSW |
14 |
14,999,078 (GRCm38) |
splice site |
probably benign |
|
|
R1829:Nek10
|
UTSW |
14 |
14,863,454 (GRCm38) |
critical splice acceptor site |
probably null |
|
|
R1831:Nek10
|
UTSW |
14 |
14,842,789 (GRCm38) |
missense |
probably benign |
|
|
R1833:Nek10
|
UTSW |
14 |
14,842,789 (GRCm38) |
missense |
probably benign |
|
|
R1990:Nek10
|
UTSW |
14 |
14,860,764 (GRCm38) |
missense |
probably benign |
|
|
R1997:Nek10
|
UTSW |
14 |
14,827,003 (GRCm38) |
missense |
probably benign |
0.09 |
|
R2011:Nek10
|
UTSW |
14 |
14,885,122 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R2158:Nek10
|
UTSW |
14 |
14,885,047 (GRCm38) |
splice site |
probably null |
|
|
R2288:Nek10
|
UTSW |
14 |
14,853,956 (GRCm38) |
nonsense |
probably null |
|
|
R2568:Nek10
|
UTSW |
14 |
14,999,112 (GRCm38) |
missense |
possibly damaging |
0.89 |
|
R2907:Nek10
|
UTSW |
14 |
14,980,613 (GRCm38) |
missense |
possibly damaging |
0.81 |
|
R2965:Nek10
|
UTSW |
14 |
14,836,202 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R3922:Nek10
|
UTSW |
14 |
14,861,585 (GRCm38) |
missense |
possibly damaging |
0.88 |
|
R4032:Nek10
|
UTSW |
14 |
14,853,877 (GRCm38) |
splice site |
probably null |
|
|
R4700:Nek10
|
UTSW |
14 |
14,842,841 (GRCm38) |
missense |
possibly damaging |
0.69 |
|
R4742:Nek10
|
UTSW |
14 |
14,861,624 (GRCm38) |
missense |
probably null |
0.03 |
|
R4785:Nek10
|
UTSW |
14 |
14,855,714 (GRCm38) |
missense |
probably benign |
|
|
R4890:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4891:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4920:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4924:Nek10
|
UTSW |
14 |
14,846,594 (GRCm38) |
splice site |
probably null |
|
|
R4928:Nek10
|
UTSW |
14 |
14,930,577 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4948:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4952:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R4953:Nek10
|
UTSW |
14 |
14,860,986 (GRCm38) |
missense |
possibly damaging |
0.47 |
|
R5092:Nek10
|
UTSW |
14 |
14,820,851 (GRCm38) |
missense |
possibly damaging |
0.81 |
|
R5097:Nek10
|
UTSW |
14 |
14,857,851 (GRCm38) |
missense |
probably benign |
0.00 |
|
R5593:Nek10
|
UTSW |
14 |
14,980,544 (GRCm38) |
nonsense |
probably null |
|
|
R5696:Nek10
|
UTSW |
14 |
14,860,736 (GRCm38) |
splice site |
probably null |
|
|
R5813:Nek10
|
UTSW |
14 |
14,986,704 (GRCm38) |
missense |
probably benign |
0.01 |
|
R5829:Nek10
|
UTSW |
14 |
14,865,404 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R5872:Nek10
|
UTSW |
14 |
14,850,896 (GRCm38) |
missense |
probably benign |
0.06 |
|
R5939:Nek10
|
UTSW |
14 |
14,931,290 (GRCm38) |
missense |
possibly damaging |
0.58 |
|
R6025:Nek10
|
UTSW |
14 |
14,865,633 (GRCm38) |
missense |
probably benign |
0.41 |
|
R6235:Nek10
|
UTSW |
14 |
14,821,113 (GRCm38) |
nonsense |
probably null |
|
|
R6539:Nek10
|
UTSW |
14 |
14,860,789 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R6542:Nek10
|
UTSW |
14 |
14,999,108 (GRCm38) |
missense |
probably benign |
0.44 |
|
R6561:Nek10
|
UTSW |
14 |
14,828,448 (GRCm38) |
missense |
possibly damaging |
0.48 |
|
R6659:Nek10
|
UTSW |
14 |
14,861,684 (GRCm38) |
missense |
probably benign |
0.29 |
|
R7039:Nek10
|
UTSW |
14 |
14,986,700 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R7039:Nek10
|
UTSW |
14 |
14,826,946 (GRCm38) |
missense |
possibly damaging |
0.63 |
|
R7102:Nek10
|
UTSW |
14 |
14,828,517 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R7185:Nek10
|
UTSW |
14 |
14,846,621 (GRCm38) |
missense |
probably benign |
0.03 |
|
R7198:Nek10
|
UTSW |
14 |
14,850,947 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R7202:Nek10
|
UTSW |
14 |
14,836,171 (GRCm38) |
missense |
probably benign |
0.01 |
|
R7345:Nek10
|
UTSW |
14 |
14,955,503 (GRCm38) |
missense |
probably benign |
|
|
R7590:Nek10
|
UTSW |
14 |
15,006,693 (GRCm38) |
makesense |
probably null |
|
|
R7593:Nek10
|
UTSW |
14 |
14,826,955 (GRCm38) |
missense |
probably benign |
0.04 |
|
R7616:Nek10
|
UTSW |
14 |
14,937,759 (GRCm38) |
missense |
probably benign |
0.27 |
|
R7635:Nek10
|
UTSW |
14 |
14,850,932 (GRCm38) |
missense |
probably benign |
0.01 |
|
R7817:Nek10
|
UTSW |
14 |
15,001,017 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7826:Nek10
|
UTSW |
14 |
14,860,846 (GRCm38) |
splice site |
probably null |
|
|
R7986:Nek10
|
UTSW |
14 |
15,001,020 (GRCm38) |
missense |
probably benign |
0.17 |
|
R8765:Nek10
|
UTSW |
14 |
14,999,104 (GRCm38) |
missense |
probably damaging |
0.97 |
|
R8856:Nek10
|
UTSW |
14 |
14,937,610 (GRCm38) |
missense |
probably damaging |
0.96 |
|
R8973:Nek10
|
UTSW |
14 |
14,931,321 (GRCm38) |
critical splice donor site |
probably null |
|
|
R9002:Nek10
|
UTSW |
14 |
14,980,590 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9088:Nek10
|
UTSW |
14 |
14,931,314 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9195:Nek10
|
UTSW |
14 |
14,821,139 (GRCm38) |
missense |
probably benign |
0.03 |
|
R9464:Nek10
|
UTSW |
14 |
14,937,766 (GRCm38) |
missense |
probably benign |
|
|
R9511:Nek10
|
UTSW |
14 |
14,828,511 (GRCm38) |
missense |
probably benign |
0.05 |
|
R9529:Nek10
|
UTSW |
14 |
14,850,833 (GRCm38) |
missense |
probably benign |
|
|
R9590:Nek10
|
UTSW |
14 |
14,853,888 (GRCm38) |
missense |
probably damaging |
1.00 |
|
Z1177:Nek10
|
UTSW |
14 |
15,001,157 (GRCm38) |
nonsense |
probably null |
|
|
Z1177:Nek10
|
UTSW |
14 |
14,853,948 (GRCm38) |
missense |
probably benign |
0.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- TTGTGTTTGAGGCACAGAAGAG -3'
(R):5'- ACGACTTTGTGCAGTTAACCTC -3'
Sequencing Primer
(F):5'- CACAGAAGAGAAAGAATAAATGGCC -3'
(R):5'- CCTGTACTGAAGAAGGCTCTG -3'
|
| Posted On |
2019-06-26 |
Incidental Mutation