Mutagenetix > Incidental Mutation

Incidental Mutation 'R7259:Stk10'

564490

Institutional Source

Beutler Lab

Gene Symbol

Stk10

Ensembl Gene

ENSMUSG00000020272

Gene Name

serine/threonine kinase 10

Synonyms

Lok, Gek1

MMRRC Submission

045351-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.107) question?

Stock #

R7259 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

32483305-32574587 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 32548497 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 355 (N355S)

Ref Sequence

ENSEMBL: ENSMUSP00000099885 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000102821]

AlphaFold

O55098

Predicted Effect

probably benign
Transcript: ENSMUST00000102821
AA Change: N355S

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000099885
Gene: ENSMUSG00000020272
AA Change: N355S

Domain	Start	End	E-Value	Type
S_TKc	36	294	8.66e-92	SMART
low complexity region	316	334	N/A	INTRINSIC
low complexity region	544	577	N/A	INTRINSIC
Pfam:PKK	586	724	1.9e-41	PFAM
Pfam:PKK	754	894	2.2e-37	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (64/64)

MGI Phenotype

FUNCTION: This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. Mice deficient for this gene product are viable, but exhibit altered integrin-mediated lymphocyte adhesion characteristics. The orthologous gene product in humans can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. [provided by RefSeq, Jul 2008]
PHENOTYPE: Targeted disruption of this gene results in enhanced cell adhesion in mitogen-stimulated T cells. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adarb2	A	G	13: 8,620,288 (GRCm39)	Y258C	probably benign	Het
Aebp2	A	G	6: 140,579,494 (GRCm39)	S244G	probably benign	Het
Agl	A	T	3: 116,578,230 (GRCm39)	Y495N	probably damaging	Het
Arhgap33	A	G	7: 30,231,625 (GRCm39)	V120A	probably damaging	Het
Asb14	C	T	14: 26,625,412 (GRCm39)	H256Y	probably benign	Het
Atp2a2	A	G	5: 122,604,132 (GRCm39)	Y434H	probably benign	Het
Atrnl1	C	T	19: 57,924,038 (GRCm39)	R1268*	probably null	Het
AW554918	A	G	18: 25,422,906 (GRCm39)		probably null	Het
Canx	A	G	11: 50,192,643 (GRCm39)	W324R	probably damaging	Het
Col9a1	A	G	1: 24,224,424 (GRCm39)	E202G	unknown	Het
Crh	C	A	3: 19,748,418 (GRCm39)	A75S	possibly damaging	Het
Ctnnal1	T	A	4: 56,817,299 (GRCm39)		probably null	Het
Dmrta2	T	A	4: 109,839,104 (GRCm39)	S284T	possibly damaging	Het
Dock1	T	C	7: 134,384,477 (GRCm39)	V680A	probably damaging	Het
Duox2	G	A	2: 122,125,657 (GRCm39)	R317C	probably damaging	Het
Elmod2	A	G	8: 84,048,138 (GRCm39)	L108P	probably damaging	Het
Exoc6b	A	G	6: 84,825,792 (GRCm39)	S540P	probably benign	Het
Fsip2	C	T	2: 82,812,474 (GRCm39)	A2931V	possibly damaging	Het
Gipc2	A	T	3: 151,833,693 (GRCm39)	I196K	probably damaging	Het
Gli2	A	G	1: 118,764,264 (GRCm39)	S1296P	probably benign	Het
Gm21103	T	A	14: 17,482,882 (GRCm39)	Q173L	probably damaging	Het
Gpatch1	A	T	7: 34,986,546 (GRCm39)		probably null	Het
Grm8	G	A	6: 27,760,175 (GRCm39)	T385I	probably null	Het
Gtf2f1	C	A	17: 57,311,562 (GRCm39)	D283Y	probably damaging	Het
Hcar1	T	A	5: 124,017,275 (GRCm39)	T139S	possibly damaging	Het
Hcrtr1	T	A	4: 130,029,611 (GRCm39)	T157S	possibly damaging	Het
Htra2	T	G	6: 83,028,520 (GRCm39)	Q436P	possibly damaging	Het
Igkv1-133	A	G	6: 67,702,522 (GRCm39)	D80G	probably benign	Het
Il21	C	A	3: 37,281,803 (GRCm39)		probably null	Het
Inpp5f	T	A	7: 128,271,681 (GRCm39)	C359S	probably benign	Het
Itgb6	A	T	2: 60,480,355 (GRCm39)	I327N	probably damaging	Het
Itgbl1	A	T	14: 124,081,316 (GRCm39)	T232S	probably damaging	Het
Kif1a	T	A	1: 93,001,532 (GRCm39)	K266*	probably null	Het
Mcm10	C	A	2: 5,011,328 (GRCm39)	E214D	probably benign	Het
Mefv	T	A	16: 3,530,917 (GRCm39)	I522F	probably damaging	Het
Naaladl2	T	A	3: 24,112,340 (GRCm39)	I581F	possibly damaging	Het
Naip6	A	T	13: 100,440,863 (GRCm39)	Y306N	probably damaging	Het
Opn1sw	T	A	6: 29,378,911 (GRCm39)	H247L	probably benign	Het
Or4c119	A	C	2: 88,986,854 (GRCm39)	F222V	probably benign	Het
Or51v15-ps1	A	T	7: 103,278,966 (GRCm39)	M67K	probably damaging	Het
Or5k1b	T	C	16: 58,580,868 (GRCm39)	T224A	possibly damaging	Het
Or6c68	T	A	10: 129,157,663 (GRCm39)	M57K	probably benign	Het
Pbxip1	C	A	3: 89,352,940 (GRCm39)	F195L	probably benign	Het
Pcdh12	A	T	18: 38,414,677 (GRCm39)	F816Y	probably benign	Het
Pi4k2b	C	A	5: 52,910,587 (GRCm39)	N290K	probably damaging	Het
Pias2	T	C	18: 77,240,416 (GRCm39)	S598P	possibly damaging	Het
Ppfia4	A	G	1: 134,240,838 (GRCm39)	V835A	probably damaging	Het
Ptch1	T	C	13: 63,721,108 (GRCm39)	K54E	not run	Het
Rnf213	A	G	11: 119,343,401 (GRCm39)	I3589V		Het
Scg2	T	C	1: 79,414,702 (GRCm39)	Y7C	probably benign	Het
Sec16a	A	G	2: 26,331,604 (GRCm39)	V137A	probably benign	Het
Setdb1	G	A	3: 95,247,224 (GRCm39)	T525I	probably benign	Het
Sgcg	A	T	14: 61,462,666 (GRCm39)	I227N	probably benign	Het
Slc35e4	A	T	11: 3,862,530 (GRCm39)		probably null	Het
Smarca2	C	T	19: 26,631,864 (GRCm39)	Q560*	probably null	Het
Supt6	A	T	11: 78,098,442 (GRCm39)	D1716E	probably damaging	Het
Taok3	C	T	5: 117,390,608 (GRCm39)	A523V	probably benign	Het
Tekt3	A	G	11: 62,974,592 (GRCm39)	T304A	possibly damaging	Het
Ttc6	G	T	12: 57,622,970 (GRCm39)	R123L	probably benign	Het
Tyw1	T	A	5: 130,296,713 (GRCm39)		probably null	Het
Ulk2	A	G	11: 61,672,909 (GRCm39)	L895P	probably damaging	Het
Unc13c	A	T	9: 73,424,645 (GRCm39)	N1990K	probably benign	Het
Vmn2r111	T	C	17: 22,778,032 (GRCm39)	N549S	possibly damaging	Het
Wipf1	A	G	2: 73,265,425 (GRCm39)	S393P	probably damaging	Het
Yju2b	T	C	8: 84,986,946 (GRCm39)	S184G	probably damaging	Het

Other mutations in Stk10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01105:Stk10	APN	11	32,527,740 (GRCm39)	missense	probably benign	0.33
IGL01285:Stk10	APN	11	32,560,653 (GRCm39)	missense	possibly damaging	0.91
IGL01983:Stk10	APN	11	32,539,460 (GRCm39)	missense	probably benign	0.05
IGL03177:Stk10	APN	11	32,564,592 (GRCm39)	missense	probably damaging	1.00
IGL03183:Stk10	APN	11	32,554,143 (GRCm39)	missense	possibly damaging	0.50
coquet	UTSW	11	32,527,764 (GRCm39)	missense
legacy	UTSW	11	32,554,166 (GRCm39)	nonsense	probably null
mignon	UTSW	11	32,537,363 (GRCm39)	missense	probably damaging	1.00
R0481_stk10_383	UTSW	11	32,564,708 (GRCm39)	missense	probably damaging	1.00
FR4976:Stk10	UTSW	11	32,564,520 (GRCm39)	critical splice acceptor site	probably benign
R0003:Stk10	UTSW	11	32,539,460 (GRCm39)	missense	probably benign	0.05
R0008:Stk10	UTSW	11	32,537,305 (GRCm39)	splice site	probably benign
R0056:Stk10	UTSW	11	32,567,851 (GRCm39)	missense	possibly damaging	0.95
R0076:Stk10	UTSW	11	32,553,722 (GRCm39)	missense	probably benign
R0227:Stk10	UTSW	11	32,567,859 (GRCm39)	missense	probably damaging	1.00
R0440:Stk10	UTSW	11	32,554,190 (GRCm39)	missense	probably damaging	1.00
R0454:Stk10	UTSW	11	32,546,724 (GRCm39)	missense	probably damaging	0.99
R0481:Stk10	UTSW	11	32,564,708 (GRCm39)	missense	probably damaging	1.00
R0504:Stk10	UTSW	11	32,567,882 (GRCm39)	missense	probably benign	0.04
R0790:Stk10	UTSW	11	32,548,653 (GRCm39)	missense	probably benign	0.00
R1439:Stk10	UTSW	11	32,567,919 (GRCm39)	missense	probably damaging	0.98
R1539:Stk10	UTSW	11	32,483,440 (GRCm39)	missense	possibly damaging	0.85
R1770:Stk10	UTSW	11	32,572,464 (GRCm39)	missense	possibly damaging	0.94
R4304:Stk10	UTSW	11	32,560,634 (GRCm39)	missense	probably damaging	0.97
R4430:Stk10	UTSW	11	32,483,552 (GRCm39)	missense	possibly damaging	0.81
R4702:Stk10	UTSW	11	32,505,172 (GRCm39)	missense	probably benign	0.28
R4797:Stk10	UTSW	11	32,548,471 (GRCm39)	missense	probably benign	0.01
R5447:Stk10	UTSW	11	32,554,166 (GRCm39)	nonsense	probably null
R5801:Stk10	UTSW	11	32,546,748 (GRCm39)	missense	probably benign	0.01
R5802:Stk10	UTSW	11	32,546,748 (GRCm39)	missense	probably benign	0.01
R6129:Stk10	UTSW	11	32,565,871 (GRCm39)	missense	probably damaging	1.00
R6154:Stk10	UTSW	11	32,553,654 (GRCm39)	splice site	probably null
R6175:Stk10	UTSW	11	32,553,761 (GRCm39)	missense	possibly damaging	0.46
R6185:Stk10	UTSW	11	32,527,749 (GRCm39)	missense	probably benign	0.13
R6520:Stk10	UTSW	11	32,538,839 (GRCm39)	missense	probably damaging	1.00
R6824:Stk10	UTSW	11	32,537,363 (GRCm39)	missense	probably damaging	1.00
R7649:Stk10	UTSW	11	32,527,764 (GRCm39)	missense
R8331:Stk10	UTSW	11	32,538,928 (GRCm39)	missense
R8847:Stk10	UTSW	11	32,539,427 (GRCm39)	missense
R9252:Stk10	UTSW	11	32,538,915 (GRCm39)	missense
R9367:Stk10	UTSW	11	32,538,878 (GRCm39)	missense
X0027:Stk10	UTSW	11	32,537,361 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CCTGTTTAAGAAAGGCTGTGG -3'
(R):5'- ATCCATCTGAATTCTGGCATCC -3'

Sequencing Primer
(F):5'- TTTAAGAAAGGCTGTGGGGAGTG -3'
(R):5'- ATTCTGGCATCCATGGACAG -3'

Posted On

2019-06-26