Mutagenetix > Incidental Mutation

Incidental Mutation 'R0582:Vmn2r27'

56464

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r27

Ensembl Gene

ENSMUSG00000072778

Gene Name

vomeronasal 2, receptor27

Synonyms

EG232367

MMRRC Submission

038772-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.061) question?

Stock #

R0582 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

124168555-124208743 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 124201249 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 236 (D236G)

Ref Sequence

ENSEMBL: ENSMUSP00000098528 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000100968]

AlphaFold

D3YUK6

Predicted Effect

probably benign
Transcript: ENSMUST00000100968
AA Change: D236G

PolyPhen 2 Score 0.019 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000098528
Gene: ENSMUSG00000072778
AA Change: D236G

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	81	475	1.1e-27	PFAM
Pfam:NCD3G	519	570	1.3e-18	PFAM
Pfam:7tm_3	603	838	2.6e-50	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.4%
20x: 94.6%

Validation Efficiency

100% (45/45)

Allele List at MGI

Other mutations in this stock

Total: 40 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb5	A	T	12: 118,904,147 (GRCm39)	M186K	probably benign	Het
Afm	T	C	5: 90,672,639 (GRCm39)		probably benign	Het
Arfgef3	G	A	10: 18,487,038 (GRCm39)	A1332V	probably damaging	Het
Atp11a	T	C	8: 12,881,214 (GRCm39)	S451P	probably benign	Het
Birc6	T	A	17: 74,950,332 (GRCm39)	V3189E	probably damaging	Het
Ccdc150	C	T	1: 54,368,670 (GRCm39)	A626V	probably benign	Het
Ccdc50	G	A	16: 27,263,409 (GRCm39)		probably benign	Het
Cntln	T	C	4: 84,802,978 (GRCm39)	S93P	probably damaging	Het
Ctnna2	C	A	6: 77,735,400 (GRCm39)	V106L	probably benign	Het
Ctnnal1	G	C	4: 56,813,228 (GRCm39)	Q668E	probably damaging	Het
Cyp1a2	G	A	9: 57,587,529 (GRCm39)		probably benign	Het
Dnah8	A	G	17: 30,937,935 (GRCm39)	D1604G	probably benign	Het
Dscaml1	A	T	9: 45,579,562 (GRCm39)	I370F	possibly damaging	Het
Duxf4	A	T	10: 58,071,508 (GRCm39)	S235R	probably benign	Het
Ears2	T	C	7: 121,654,881 (GRCm39)	E129G	probably benign	Het
Igsf10	T	C	3: 59,227,188 (GRCm39)	I2162V	probably benign	Het
Ints9	C	T	14: 65,217,598 (GRCm39)	P42S	probably damaging	Het
Ipp	T	C	4: 116,372,664 (GRCm39)	L231S	probably damaging	Het
Lyn	T	A	4: 3,743,296 (GRCm39)	L72Q	probably damaging	Het
Nfe2l2	T	A	2: 75,507,112 (GRCm39)	E329D	probably damaging	Het
Or2h2c	G	C	17: 37,422,347 (GRCm39)	L176V	probably benign	Het
Or52a24	G	A	7: 103,381,880 (GRCm39)	C249Y	possibly damaging	Het
Pdyn	A	C	2: 129,531,658 (GRCm39)	L44R	probably damaging	Het
Pkd1l1	A	G	11: 8,881,699 (GRCm39)		probably benign	Het
Prpf40a	A	T	2: 53,035,704 (GRCm39)	F695L	probably damaging	Het
Rnf217	A	G	10: 31,484,763 (GRCm39)	Y140H	possibly damaging	Het
Sema6c	C	T	3: 95,076,508 (GRCm39)	R265C	probably damaging	Het
Slc7a8	C	A	14: 54,995,901 (GRCm39)	C167F	probably damaging	Het
Snap47	A	T	11: 59,319,259 (GRCm39)	L293*	probably null	Het
Snx3	A	T	10: 42,409,276 (GRCm39)		probably benign	Het
Sycp2l	T	A	13: 41,291,431 (GRCm39)		probably benign	Het
Taar3	A	G	10: 23,825,715 (GRCm39)	Y87C	probably damaging	Het
Tm4sf4	T	C	3: 57,341,278 (GRCm39)		probably benign	Het
Tssc4	T	C	7: 142,624,246 (GRCm39)	S185P	probably damaging	Het
Ttc28	G	T	5: 111,331,162 (GRCm39)	A430S	probably damaging	Het
Ulbp3	G	A	10: 3,075,082 (GRCm39)		noncoding transcript	Het
Vps54	G	T	11: 21,250,137 (GRCm39)	D508Y	probably damaging	Het
Wdr53	G	A	16: 32,070,726 (GRCm39)	V24M	probably damaging	Het
Xirp2	T	G	2: 67,339,210 (GRCm39)	L484V	probably benign	Het
Zfyve26	T	C	12: 79,292,996 (GRCm39)	D2051G	probably damaging	Het

Other mutations in Vmn2r27

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01285:Vmn2r27	APN	6	124,169,370 (GRCm39)	missense	possibly damaging	0.86
IGL01388:Vmn2r27	APN	6	124,200,791 (GRCm39)	missense	possibly damaging	0.55
IGL01923:Vmn2r27	APN	6	124,177,484 (GRCm39)	missense	probably benign	0.20
IGL01954:Vmn2r27	APN	6	124,169,207 (GRCm39)	missense	probably damaging	1.00
IGL02105:Vmn2r27	APN	6	124,174,308 (GRCm39)	splice site	probably benign
IGL02586:Vmn2r27	APN	6	124,201,434 (GRCm39)	nonsense	probably null
IGL03130:Vmn2r27	APN	6	124,169,276 (GRCm39)	missense	possibly damaging	0.82
IGL03330:Vmn2r27	APN	6	124,207,139 (GRCm39)	nonsense	probably null
R0124:Vmn2r27	UTSW	6	124,208,578 (GRCm39)	missense	probably benign
R0234:Vmn2r27	UTSW	6	124,208,578 (GRCm39)	missense	probably benign
R0234:Vmn2r27	UTSW	6	124,208,578 (GRCm39)	missense	probably benign
R0384:Vmn2r27	UTSW	6	124,200,871 (GRCm39)	missense	probably benign	0.01
R0733:Vmn2r27	UTSW	6	124,169,147 (GRCm39)	missense	probably benign	0.18
R0738:Vmn2r27	UTSW	6	124,200,661 (GRCm39)	missense	possibly damaging	0.48
R0835:Vmn2r27	UTSW	6	124,177,583 (GRCm39)	missense	probably damaging	0.99
R1183:Vmn2r27	UTSW	6	124,177,491 (GRCm39)	missense	probably benign
R1401:Vmn2r27	UTSW	6	124,168,591 (GRCm39)	nonsense	probably null
R1484:Vmn2r27	UTSW	6	124,177,474 (GRCm39)	missense	probably damaging	0.96
R1536:Vmn2r27	UTSW	6	124,177,649 (GRCm39)	missense	probably damaging	1.00
R1539:Vmn2r27	UTSW	6	124,168,730 (GRCm39)	missense	probably damaging	1.00
R1565:Vmn2r27	UTSW	6	124,208,593 (GRCm39)	missense	probably benign
R1595:Vmn2r27	UTSW	6	124,208,574 (GRCm39)	missense	probably benign	0.00
R1614:Vmn2r27	UTSW	6	124,200,893 (GRCm39)	missense	probably benign	0.01
R1742:Vmn2r27	UTSW	6	124,177,636 (GRCm39)	missense	possibly damaging	0.48
R1816:Vmn2r27	UTSW	6	124,207,330 (GRCm39)	nonsense	probably null
R1822:Vmn2r27	UTSW	6	124,208,593 (GRCm39)	missense	probably benign
R1824:Vmn2r27	UTSW	6	124,208,593 (GRCm39)	missense	probably benign
R1870:Vmn2r27	UTSW	6	124,201,170 (GRCm39)	missense	probably benign	0.11
R1942:Vmn2r27	UTSW	6	124,200,722 (GRCm39)	missense	probably damaging	1.00
R1962:Vmn2r27	UTSW	6	124,200,793 (GRCm39)	missense	possibly damaging	0.70
R2069:Vmn2r27	UTSW	6	124,201,442 (GRCm39)	missense	probably damaging	1.00
R2075:Vmn2r27	UTSW	6	124,177,510 (GRCm39)	missense	possibly damaging	0.85
R2379:Vmn2r27	UTSW	6	124,201,342 (GRCm39)	missense	possibly damaging	0.89
R3748:Vmn2r27	UTSW	6	124,207,351 (GRCm39)	missense	probably benign	0.35
R4384:Vmn2r27	UTSW	6	124,201,115 (GRCm39)	missense	probably benign	0.05
R4392:Vmn2r27	UTSW	6	124,207,135 (GRCm39)	missense	probably benign	0.01
R4758:Vmn2r27	UTSW	6	124,208,596 (GRCm39)	missense	possibly damaging	0.87
R5018:Vmn2r27	UTSW	6	124,201,141 (GRCm39)	missense	probably benign	0.02
R5235:Vmn2r27	UTSW	6	124,169,013 (GRCm39)	missense	probably damaging	0.99
R5718:Vmn2r27	UTSW	6	124,169,103 (GRCm39)	missense	possibly damaging	0.66
R5859:Vmn2r27	UTSW	6	124,177,647 (GRCm39)	missense	probably damaging	1.00
R5958:Vmn2r27	UTSW	6	124,208,686 (GRCm39)	missense	probably benign	0.00
R6044:Vmn2r27	UTSW	6	124,208,731 (GRCm39)	missense	probably benign
R6086:Vmn2r27	UTSW	6	124,168,958 (GRCm39)	missense	probably damaging	1.00
R6396:Vmn2r27	UTSW	6	124,201,125 (GRCm39)	nonsense	probably null
R6546:Vmn2r27	UTSW	6	124,169,369 (GRCm39)	missense	possibly damaging	0.49
R6746:Vmn2r27	UTSW	6	124,177,552 (GRCm39)	missense	possibly damaging	0.47
R6976:Vmn2r27	UTSW	6	124,201,312 (GRCm39)	nonsense	probably null
R7091:Vmn2r27	UTSW	6	124,200,904 (GRCm39)	missense	possibly damaging	0.85
R7145:Vmn2r27	UTSW	6	124,168,711 (GRCm39)	missense	probably benign
R7176:Vmn2r27	UTSW	6	124,168,995 (GRCm39)	missense	probably benign	0.01
R7382:Vmn2r27	UTSW	6	124,174,276 (GRCm39)	missense	probably damaging	1.00
R7482:Vmn2r27	UTSW	6	124,201,220 (GRCm39)	missense	probably damaging	1.00
R7853:Vmn2r27	UTSW	6	124,168,980 (GRCm39)	missense	probably damaging	1.00
R7859:Vmn2r27	UTSW	6	124,201,201 (GRCm39)	missense	probably benign	0.00
R7959:Vmn2r27	UTSW	6	124,169,040 (GRCm39)	missense	probably benign
R8266:Vmn2r27	UTSW	6	124,168,937 (GRCm39)	missense	probably benign	0.00
R8353:Vmn2r27	UTSW	6	124,169,404 (GRCm39)	missense	probably damaging	0.99
R8394:Vmn2r27	UTSW	6	124,168,776 (GRCm39)	missense	possibly damaging	0.71
R8463:Vmn2r27	UTSW	6	124,169,168 (GRCm39)	missense	probably damaging	1.00
R8477:Vmn2r27	UTSW	6	124,201,200 (GRCm39)	missense	probably benign	0.11
R8705:Vmn2r27	UTSW	6	124,207,188 (GRCm39)	missense	probably damaging	1.00
R8752:Vmn2r27	UTSW	6	124,201,018 (GRCm39)	missense	probably benign	0.00
R9109:Vmn2r27	UTSW	6	124,174,224 (GRCm39)	missense	possibly damaging	0.95
R9140:Vmn2r27	UTSW	6	124,169,207 (GRCm39)	missense	probably damaging	1.00
R9157:Vmn2r27	UTSW	6	124,201,244 (GRCm39)	missense	probably benign	0.09
R9431:Vmn2r27	UTSW	6	124,168,856 (GRCm39)	missense	probably damaging	1.00
R9477:Vmn2r27	UTSW	6	124,168,910 (GRCm39)	missense	probably damaging	0.99
R9758:Vmn2r27	UTSW	6	124,168,637 (GRCm39)	missense	possibly damaging	0.89
Z1177:Vmn2r27	UTSW	6	124,168,860 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATGTGGACACTCAAATACGATGGACC -3'
(R):5'- GTACACAGCGATGAGTCAGTGACATAG -3'

Sequencing Primer
(F):5'- TTCTGAGAAACTCCTTGAATCCCAG -3'
(R):5'- TTTCTCCTAGAGTACACAAACAGAG -3'

Posted On

2013-07-11