Mutagenetix > Incidental Mutation

Incidental Mutation 'R0582:Vmn2r27'

56464

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r27

Ensembl Gene

ENSMUSG00000072778

Gene Name

vomeronasal 2, receptor27

Synonyms

EG232367

MMRRC Submission

038772-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.077) question?

Stock #

R0582 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

124191596-124231784 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 124224290 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 236 (D236G)

Ref Sequence

ENSEMBL: ENSMUSP00000098528 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000100968]

AlphaFold

D3YUK6

Predicted Effect

probably benign
Transcript: ENSMUST00000100968
AA Change: D236G

PolyPhen 2 Score 0.019 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000098528
Gene: ENSMUSG00000072778
AA Change: D236G

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	81	475	1.1e-27	PFAM
Pfam:NCD3G	519	570	1.3e-18	PFAM
Pfam:7tm_3	603	838	2.6e-50	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.4%
20x: 94.6%

Validation Efficiency

100% (45/45)

Allele List at MGI

Other mutations in this stock

Total: 40 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9230019H11Rik	G	A	10: 3,125,082		noncoding transcript	Het
Abcb5	A	T	12: 118,940,412	M186K	probably benign	Het
Afm	T	C	5: 90,524,780		probably benign	Het
Arfgef3	G	A	10: 18,611,290	A1332V	probably damaging	Het
Atp11a	T	C	8: 12,831,214	S451P	probably benign	Het
Birc6	T	A	17: 74,643,337	V3189E	probably damaging	Het
Ccdc150	C	T	1: 54,329,511	A626V	probably benign	Het
Ccdc50	G	A	16: 27,444,659		probably benign	Het
Cntln	T	C	4: 84,884,741	S93P	probably damaging	Het
Ctnna2	C	A	6: 77,758,417	V106L	probably benign	Het
Ctnnal1	G	C	4: 56,813,228	Q668E	probably damaging	Het
Cyp1a2	G	A	9: 57,680,246		probably benign	Het
Dnah8	A	G	17: 30,718,961	D1604G	probably benign	Het
Dscaml1	A	T	9: 45,668,264	I370F	possibly damaging	Het
Ears2	T	C	7: 122,055,658	E129G	probably benign	Het
Gm4981	A	T	10: 58,235,686	S235R	probably benign	Het
Igsf10	T	C	3: 59,319,767	I2162V	probably benign	Het
Ints9	C	T	14: 64,980,149	P42S	probably damaging	Het
Ipp	T	C	4: 116,515,467	L231S	probably damaging	Het
Lyn	T	A	4: 3,743,296	L72Q	probably damaging	Het
Nfe2l2	T	A	2: 75,676,768	E329D	probably damaging	Het
Olfr628	G	A	7: 103,732,673	C249Y	possibly damaging	Het
Olfr92	G	C	17: 37,111,455	L176V	probably benign	Het
Pdyn	A	C	2: 129,689,738	L44R	probably damaging	Het
Pkd1l1	A	G	11: 8,931,699		probably benign	Het
Prpf40a	A	T	2: 53,145,692	F695L	probably damaging	Het
Rnf217	A	G	10: 31,608,767	Y140H	possibly damaging	Het
Sema6c	C	T	3: 95,169,197	R265C	probably damaging	Het
Slc7a8	C	A	14: 54,758,444	C167F	probably damaging	Het
Snap47	A	T	11: 59,428,433	L293*	probably null	Het
Snx3	A	T	10: 42,533,280		probably benign	Het
Sycp2l	T	A	13: 41,137,955		probably benign	Het
Taar3	A	G	10: 23,949,817	Y87C	probably damaging	Het
Tm4sf4	T	C	3: 57,433,857		probably benign	Het
Tssc4	T	C	7: 143,070,509	S185P	probably damaging	Het
Ttc28	G	T	5: 111,183,296	A430S	probably damaging	Het
Vps54	G	T	11: 21,300,137	D508Y	probably damaging	Het
Wdr53	G	A	16: 32,251,908	V24M	probably damaging	Het
Xirp2	T	G	2: 67,508,866	L484V	probably benign	Het
Zfyve26	T	C	12: 79,246,222	D2051G	probably damaging	Het

Other mutations in Vmn2r27

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01285:Vmn2r27	APN	6	124192411	missense	possibly damaging	0.86
IGL01388:Vmn2r27	APN	6	124223832	missense	possibly damaging	0.55
IGL01923:Vmn2r27	APN	6	124200525	missense	probably benign	0.20
IGL01954:Vmn2r27	APN	6	124192248	missense	probably damaging	1.00
IGL02105:Vmn2r27	APN	6	124197349	splice site	probably benign
IGL02586:Vmn2r27	APN	6	124224475	nonsense	probably null
IGL03130:Vmn2r27	APN	6	124192317	missense	possibly damaging	0.82
IGL03330:Vmn2r27	APN	6	124230180	nonsense	probably null
R0124:Vmn2r27	UTSW	6	124231619	missense	probably benign
R0234:Vmn2r27	UTSW	6	124231619	missense	probably benign
R0234:Vmn2r27	UTSW	6	124231619	missense	probably benign
R0384:Vmn2r27	UTSW	6	124223912	missense	probably benign	0.01
R0733:Vmn2r27	UTSW	6	124192188	missense	probably benign	0.18
R0738:Vmn2r27	UTSW	6	124223702	missense	possibly damaging	0.48
R0835:Vmn2r27	UTSW	6	124200624	missense	probably damaging	0.99
R1183:Vmn2r27	UTSW	6	124200532	missense	probably benign
R1401:Vmn2r27	UTSW	6	124191632	nonsense	probably null
R1484:Vmn2r27	UTSW	6	124200515	missense	probably damaging	0.96
R1536:Vmn2r27	UTSW	6	124200690	missense	probably damaging	1.00
R1539:Vmn2r27	UTSW	6	124191771	missense	probably damaging	1.00
R1565:Vmn2r27	UTSW	6	124231634	missense	probably benign
R1595:Vmn2r27	UTSW	6	124231615	missense	probably benign	0.00
R1614:Vmn2r27	UTSW	6	124223934	missense	probably benign	0.01
R1742:Vmn2r27	UTSW	6	124200677	missense	possibly damaging	0.48
R1816:Vmn2r27	UTSW	6	124230371	nonsense	probably null
R1822:Vmn2r27	UTSW	6	124231634	missense	probably benign
R1824:Vmn2r27	UTSW	6	124231634	missense	probably benign
R1870:Vmn2r27	UTSW	6	124224211	missense	probably benign	0.11
R1942:Vmn2r27	UTSW	6	124223763	missense	probably damaging	1.00
R1962:Vmn2r27	UTSW	6	124223834	missense	possibly damaging	0.70
R2069:Vmn2r27	UTSW	6	124224483	missense	probably damaging	1.00
R2075:Vmn2r27	UTSW	6	124200551	missense	possibly damaging	0.85
R2379:Vmn2r27	UTSW	6	124224383	missense	possibly damaging	0.89
R3748:Vmn2r27	UTSW	6	124230392	missense	probably benign	0.35
R4384:Vmn2r27	UTSW	6	124224156	missense	probably benign	0.05
R4392:Vmn2r27	UTSW	6	124230176	missense	probably benign	0.01
R4758:Vmn2r27	UTSW	6	124231637	missense	possibly damaging	0.87
R5018:Vmn2r27	UTSW	6	124224182	missense	probably benign	0.02
R5235:Vmn2r27	UTSW	6	124192054	missense	probably damaging	0.99
R5718:Vmn2r27	UTSW	6	124192144	missense	possibly damaging	0.66
R5859:Vmn2r27	UTSW	6	124200688	missense	probably damaging	1.00
R5958:Vmn2r27	UTSW	6	124231727	missense	probably benign	0.00
R6044:Vmn2r27	UTSW	6	124231772	missense	probably benign
R6086:Vmn2r27	UTSW	6	124191999	missense	probably damaging	1.00
R6396:Vmn2r27	UTSW	6	124224166	nonsense	probably null
R6546:Vmn2r27	UTSW	6	124192410	missense	possibly damaging	0.49
R6746:Vmn2r27	UTSW	6	124200593	missense	possibly damaging	0.47
R6976:Vmn2r27	UTSW	6	124224353	nonsense	probably null
R7091:Vmn2r27	UTSW	6	124223945	missense	possibly damaging	0.85
R7145:Vmn2r27	UTSW	6	124191752	missense	probably benign
R7176:Vmn2r27	UTSW	6	124192036	missense	probably benign	0.01
R7382:Vmn2r27	UTSW	6	124197317	missense	probably damaging	1.00
R7482:Vmn2r27	UTSW	6	124224261	missense	probably damaging	1.00
R7853:Vmn2r27	UTSW	6	124192021	missense	probably damaging	1.00
R7859:Vmn2r27	UTSW	6	124224242	missense	probably benign	0.00
R7959:Vmn2r27	UTSW	6	124192081	missense	probably benign
R8266:Vmn2r27	UTSW	6	124191978	missense	probably benign	0.00
R8353:Vmn2r27	UTSW	6	124192445	missense	probably damaging	0.99
R8394:Vmn2r27	UTSW	6	124191817	missense	possibly damaging	0.71
R8463:Vmn2r27	UTSW	6	124192209	missense	probably damaging	1.00
R8477:Vmn2r27	UTSW	6	124224241	missense	probably benign	0.11
R8705:Vmn2r27	UTSW	6	124230229	missense	probably damaging	1.00
R8752:Vmn2r27	UTSW	6	124224059	missense	probably benign	0.00
R9109:Vmn2r27	UTSW	6	124197265	missense	possibly damaging	0.95
R9140:Vmn2r27	UTSW	6	124192248	missense	probably damaging	1.00
R9157:Vmn2r27	UTSW	6	124224285	missense	probably benign	0.09
R9431:Vmn2r27	UTSW	6	124191897	missense	probably damaging	1.00
R9477:Vmn2r27	UTSW	6	124191951	missense	probably damaging	0.99
R9758:Vmn2r27	UTSW	6	124191678	missense	possibly damaging	0.89
Z1177:Vmn2r27	UTSW	6	124191901	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATGTGGACACTCAAATACGATGGACC -3'
(R):5'- GTACACAGCGATGAGTCAGTGACATAG -3'

Sequencing Primer
(F):5'- TTCTGAGAAACTCCTTGAATCCCAG -3'
(R):5'- TTTCTCCTAGAGTACACAAACAGAG -3'

Posted On

2013-07-11