Incidental Mutation 'R0583:Crlf3'
ID 56520
Institutional Source Beutler Lab
Gene Symbol Crlf3
Ensembl Gene ENSMUSG00000017561
Gene Name cytokine receptor-like factor 3
Synonyms Creme9, cytor4
MMRRC Submission 038773-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R0583 (G1)
Quality Score 166
Status Not validated
Chromosome 11
Chromosomal Location 79937319-79971817 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 79950107 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Histidine to Glutamine at position 174 (H174Q)
Ref Sequence ENSEMBL: ENSMUSP00000099523 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000061283] [ENSMUST00000103233] [ENSMUST00000177825] [ENSMUST00000178893] [ENSMUST00000179855]
AlphaFold Q9Z2L7
Predicted Effect probably damaging
Transcript: ENSMUST00000061283
AA Change: H174Q

PolyPhen 2 Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000060028
Gene: ENSMUSG00000017561
AA Change: H174Q

DomainStartEndE-ValueType
coiled coil region 10 43 N/A INTRINSIC
low complexity region 112 124 N/A INTRINSIC
FN3 179 260 1.66e0 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000092858
Predicted Effect probably damaging
Transcript: ENSMUST00000103233
AA Change: H174Q

PolyPhen 2 Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000099523
Gene: ENSMUSG00000017561
AA Change: H174Q

DomainStartEndE-ValueType
coiled coil region 10 43 N/A INTRINSIC
low complexity region 112 124 N/A INTRINSIC
FN3 179 260 1.66e0 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000147164
Predicted Effect probably benign
Transcript: ENSMUST00000177825
SMART Domains Protein: ENSMUSP00000136473
Gene: ENSMUSG00000017561

DomainStartEndE-ValueType
coiled coil region 10 43 N/A INTRINSIC
low complexity region 112 124 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000178280
Predicted Effect probably benign
Transcript: ENSMUST00000178893
SMART Domains Protein: ENSMUSP00000137371
Gene: ENSMUSG00000017561

DomainStartEndE-ValueType
coiled coil region 10 39 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000179855
Coding Region Coverage
  • 1x: 99.4%
  • 3x: 98.8%
  • 10x: 97.1%
  • 20x: 93.2%
Validation Efficiency
MGI Phenotype FUNCTION: This gene encodes a cytokine receptor-like factor that contains a fibronectin type 3 domain. The encoded protein may act as a negative regulator of the cell cycle. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit decreased lean body mass, decreased platelet cell number and increased circulating fructosamine level. [provided by MGI curators]
Allele List at MGI

All alleles(39) : Targeted, other(2) Gene trapped(37)
Other mutations in this stock
Total: 52 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700017B05Rik A G 9: 57,164,926 (GRCm39) S483P probably benign Het
5730480H06Rik A G 5: 48,537,470 (GRCm39) H169R probably damaging Het
Actn1 T A 12: 80,245,803 (GRCm39) I127F probably damaging Het
Cadm3 T G 1: 173,168,738 (GRCm39) T277P probably benign Het
Cast T C 13: 74,861,797 (GRCm39) T629A probably damaging Het
Cblc C A 7: 19,526,486 (GRCm39) C201F probably benign Het
Ccdc154 T A 17: 25,387,398 (GRCm39) D375E possibly damaging Het
Cdk6 A G 5: 3,523,183 (GRCm39) D201G probably damaging Het
Cep95 T C 11: 106,705,449 (GRCm39) V478A probably benign Het
Ciita T C 16: 10,341,668 (GRCm39) probably null Het
Clec4e A G 6: 123,260,653 (GRCm39) F135S probably damaging Het
Cntn6 A G 6: 104,753,275 (GRCm39) D337G possibly damaging Het
Cyb5r1 T A 1: 134,335,339 (GRCm39) F93I probably damaging Het
Dop1b T C 16: 93,552,374 (GRCm39) I271T probably benign Het
Duxf1 G A 10: 58,059,210 (GRCm39) L515F probably damaging Het
Fcho1 T C 8: 72,168,369 (GRCm39) Y218C probably damaging Het
Fhad1 C T 4: 141,631,301 (GRCm39) M1297I probably benign Het
Igdcc4 T C 9: 65,029,095 (GRCm39) V244A possibly damaging Het
Ikzf5 A G 7: 130,993,514 (GRCm39) probably null Het
Ilvbl T A 10: 78,419,101 (GRCm39) V450E probably damaging Het
Kcns3 T G 12: 11,141,479 (GRCm39) N407H probably damaging Het
Klhl11 T C 11: 100,355,150 (GRCm39) K224E possibly damaging Het
Klra17 T A 6: 129,845,656 (GRCm39) D186V probably damaging Het
Lrrc37a T C 11: 103,389,263 (GRCm39) D2054G probably benign Het
Mef2a G T 7: 66,884,896 (GRCm39) S406* probably null Het
Mrgbp A G 2: 180,226,239 (GRCm39) N104S probably benign Het
Mroh2a GT GTT 1: 88,183,888 (GRCm39) probably null Het
Muc5ac C A 7: 141,361,345 (GRCm39) T1552N probably damaging Het
Muc5b T A 7: 141,410,435 (GRCm39) Y1269* probably null Het
Myef2 T C 2: 124,939,901 (GRCm39) probably null Het
Myg1 C T 15: 102,246,225 (GRCm39) Q367* probably null Het
Nalcn T C 14: 123,531,755 (GRCm39) N1365S possibly damaging Het
Nfu1 T C 6: 86,986,934 (GRCm39) C18R probably benign Het
Nkx2-6 A T 14: 69,412,228 (GRCm39) Q132L probably damaging Het
Or10a3b C T 7: 108,444,621 (GRCm39) A199T possibly damaging Het
Or8k38 T A 2: 86,488,704 (GRCm39) I33F probably benign Het
Pak3 TTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC TTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC X: 142,526,889 (GRCm39) probably benign Het
Prh1 A T 6: 132,548,796 (GRCm39) Q101L unknown Het
Ribc2 A T 15: 85,017,115 (GRCm39) probably null Het
Rnf19a C A 15: 36,253,151 (GRCm39) R396L probably damaging Het
Sdad1 A G 5: 92,452,923 (GRCm39) I105T probably damaging Het
Sec24b G T 3: 129,834,960 (GRCm39) Y79* probably null Het
Tatdn2 A G 6: 113,679,486 (GRCm39) E277G possibly damaging Het
Tex10 A C 4: 48,451,952 (GRCm39) F725V probably damaging Het
Themis3 T C 17: 66,866,748 (GRCm39) D164G probably benign Het
Ubxn7 T C 16: 32,194,732 (GRCm39) W220R probably damaging Het
Usp33 C A 3: 152,073,891 (GRCm39) R246S probably damaging Het
Vmn2r102 T A 17: 19,897,043 (GRCm39) V130E probably benign Het
Vmn2r112 C T 17: 22,837,930 (GRCm39) P797L probably damaging Het
Vmn2r114 ATTT ATT 17: 23,509,906 (GRCm39) probably null Het
Yme1l1 T C 2: 23,076,262 (GRCm39) V340A probably damaging Het
Zfta A G 19: 7,397,639 (GRCm39) D62G probably damaging Het
Other mutations in Crlf3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00690:Crlf3 APN 11 79,950,163 (GRCm39) missense probably damaging 1.00
IGL00835:Crlf3 APN 11 79,938,501 (GRCm39) missense probably benign 0.03
IGL01520:Crlf3 APN 11 79,950,972 (GRCm39) missense probably benign 0.01
IGL01998:Crlf3 APN 11 79,948,845 (GRCm39) unclassified probably benign
IGL02622:Crlf3 APN 11 79,950,150 (GRCm39) missense probably damaging 1.00
IGL02748:Crlf3 APN 11 79,950,145 (GRCm39) missense probably damaging 0.98
R0064:Crlf3 UTSW 11 79,948,728 (GRCm39) missense possibly damaging 0.81
R0064:Crlf3 UTSW 11 79,948,728 (GRCm39) missense possibly damaging 0.81
R0076:Crlf3 UTSW 11 79,947,427 (GRCm39) unclassified probably benign
R1753:Crlf3 UTSW 11 79,948,698 (GRCm39) missense probably damaging 1.00
R2125:Crlf3 UTSW 11 79,950,081 (GRCm39) missense probably benign 0.21
R2571:Crlf3 UTSW 11 79,938,339 (GRCm39) missense probably benign 0.11
R5894:Crlf3 UTSW 11 79,948,678 (GRCm39) missense probably damaging 1.00
R6086:Crlf3 UTSW 11 79,939,436 (GRCm39) missense possibly damaging 0.81
R7214:Crlf3 UTSW 11 79,955,216 (GRCm39) missense possibly damaging 0.92
R7238:Crlf3 UTSW 11 79,947,351 (GRCm39) missense possibly damaging 0.94
R7511:Crlf3 UTSW 11 79,954,812 (GRCm39) splice site probably null
R8745:Crlf3 UTSW 11 79,955,100 (GRCm39) missense probably damaging 1.00
R8872:Crlf3 UTSW 11 79,938,440 (GRCm39) missense
R9297:Crlf3 UTSW 11 79,950,031 (GRCm39) missense probably damaging 1.00
X0064:Crlf3 UTSW 11 79,955,013 (GRCm39) splice site probably null
Predicted Primers PCR Primer
(F):5'- GCTGAAAATGCACTATCATCTTCTGGC -3'
(R):5'- GTGTTCTTCTAGCGTGTGACCAGTC -3'

Sequencing Primer
(F):5'- agaaagaaaggaaggatggaagg -3'
(R):5'- GCGTGTGACCAGTCTAAAATAGTC -3'
Posted On 2013-07-11