Incidental Mutation 'R0584:H1f7'
ID 56581
Institutional Source Beutler Lab
Gene Symbol H1f7
Ensembl Gene ENSMUSG00000048077
Gene Name H1.7 linker histone
Synonyms H1T2, 1700026P10Rik, H1-7, H1fnt
MMRRC Submission 038774-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.224) question?
Stock # R0584 (G1)
Quality Score 174
Status Validated
Chromosome 15
Chromosomal Location 98153867-98155174 bp(-) (GRCm39)
Type of Mutation nonsense
DNA Base Change (assembly) C to A at 98154958 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Glutamic Acid to Stop codon at position 64 (E64*)
Ref Sequence ENSEMBL: ENSMUSP00000127616 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000060855]
AlphaFold Q8CJI4
Predicted Effect probably null
Transcript: ENSMUST00000060855
AA Change: E64*
SMART Domains Protein: ENSMUSP00000127616
Gene: ENSMUSG00000048077
AA Change: E64*

DomainStartEndE-ValueType
low complexity region 134 245 N/A INTRINSIC
low complexity region 253 276 N/A INTRINSIC
Meta Mutation Damage Score 0.9716 question?
Coding Region Coverage
  • 1x: 99.4%
  • 3x: 98.8%
  • 10x: 97.2%
  • 20x: 93.7%
Validation Efficiency 98% (47/48)
MGI Phenotype FUNCTION: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family. This gene encodes a testis specific protein that is required for spermatogenesis and male fertility. [provided by RefSeq, Oct 2015]
PHENOTYPE: Homozygous null male mice display reduced fertility with asthenozoospermia, oligozoospermia, and teratozoospermia. [provided by MGI curators]
Allele List at MGI

All alleles(2) : Targeted, knock-out(2)
Other mutations in this stock
Total: 45 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca7 T C 10: 79,847,564 (GRCm39) S1745P probably damaging Het
Agfg2 G A 5: 137,665,992 (GRCm39) T89I probably damaging Het
Agtr1a A G 13: 30,565,017 (GRCm39) I27M probably damaging Het
Armh1 A T 4: 117,087,047 (GRCm39) L206Q probably damaging Het
Asxl2 A T 12: 3,546,632 (GRCm39) E472V probably damaging Het
Atp2c2 T A 8: 120,465,157 (GRCm39) V313E probably benign Het
Casp12 A G 9: 5,352,268 (GRCm39) I87V probably null Het
Ccl25 C T 8: 4,404,085 (GRCm39) probably benign Het
Col9a1 T C 1: 24,263,571 (GRCm39) probably benign Het
Cyth4 A G 15: 78,494,078 (GRCm39) probably null Het
Dnah9 A G 11: 65,881,315 (GRCm39) Y2587H probably damaging Het
Dppa3 T C 6: 122,606,951 (GRCm39) I147T probably benign Het
Fam120b T C 17: 15,622,384 (GRCm39) S121P probably damaging Het
Fam209 C T 2: 172,316,081 (GRCm39) T152M probably benign Het
Fam98a A G 17: 75,851,772 (GRCm39) L103P probably damaging Het
Fcho1 T C 8: 72,168,369 (GRCm39) Y218C probably damaging Het
Fitm1 T C 14: 55,814,113 (GRCm39) V203A probably benign Het
Gcn1 G A 5: 115,733,074 (GRCm39) R1037Q probably damaging Het
Gm11563 A G 11: 99,549,451 (GRCm39) I101T unknown Het
Gpd1l A G 9: 114,743,412 (GRCm39) F163L probably damaging Het
Grp C A 18: 66,006,766 (GRCm39) A30E possibly damaging Het
Icosl T C 10: 77,907,709 (GRCm39) Y90H possibly damaging Het
Itsn2 A G 12: 4,747,180 (GRCm39) T1194A probably benign Het
Lrp6 A G 6: 134,433,039 (GRCm39) S1431P probably damaging Het
Ltbp1 T C 17: 75,670,467 (GRCm39) S1503P probably damaging Het
Mef2a G T 7: 66,884,896 (GRCm39) S406* probably null Het
Myh13 G T 11: 67,251,200 (GRCm39) E1360* probably null Het
Nop58 A G 1: 59,745,919 (GRCm39) D400G probably benign Het
Or5p79 T C 7: 108,221,622 (GRCm39) L201P probably benign Het
Or6c205 T C 10: 129,086,817 (GRCm39) V138A probably benign Het
Oscp1 A G 4: 125,977,387 (GRCm39) probably null Het
Pde9a A T 17: 31,678,951 (GRCm39) Y264F probably damaging Het
Pkhd1 T A 1: 20,309,660 (GRCm39) K2763* probably null Het
Prkci T A 3: 31,079,289 (GRCm39) C42* probably null Het
Ptprr T C 10: 116,087,063 (GRCm39) V270A probably damaging Het
Rsf1 T A 7: 97,311,335 (GRCm39) N688K possibly damaging Het
S1pr3 A T 13: 51,573,697 (GRCm39) M293L probably benign Het
Scn10a A C 9: 119,499,597 (GRCm39) L232R probably damaging Het
Sirt5 T A 13: 43,548,204 (GRCm39) probably null Het
Sp6 C T 11: 96,913,091 (GRCm39) T268M probably damaging Het
Spag5 A G 11: 78,194,921 (GRCm39) N76S possibly damaging Het
Tecta T C 9: 42,259,204 (GRCm39) N1560D possibly damaging Het
Togaram1 T C 12: 65,014,279 (GRCm39) L510P probably damaging Het
Umps A G 16: 33,779,494 (GRCm39) I401T probably damaging Het
Vars2 A G 17: 35,977,578 (GRCm39) V118A possibly damaging Het
Other mutations in H1f7
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01693:H1f7 APN 15 98,154,262 (GRCm39) missense unknown
IGL02088:H1f7 APN 15 98,155,059 (GRCm39) missense probably damaging 0.98
IGL02322:H1f7 APN 15 98,154,757 (GRCm39) missense possibly damaging 0.94
IGL02697:H1f7 APN 15 98,155,050 (GRCm39) missense probably benign 0.02
F2404:H1f7 UTSW 15 98,155,090 (GRCm39) missense possibly damaging 0.95
R1022:H1f7 UTSW 15 98,154,636 (GRCm39) missense unknown
R1024:H1f7 UTSW 15 98,154,636 (GRCm39) missense unknown
R1462:H1f7 UTSW 15 98,154,454 (GRCm39) missense unknown
R1462:H1f7 UTSW 15 98,154,454 (GRCm39) missense unknown
R1665:H1f7 UTSW 15 98,154,796 (GRCm39) missense probably benign 0.00
R1733:H1f7 UTSW 15 98,154,016 (GRCm39) missense unknown
R2213:H1f7 UTSW 15 98,154,219 (GRCm39) missense unknown
R4683:H1f7 UTSW 15 98,154,921 (GRCm39) missense probably damaging 0.99
R4969:H1f7 UTSW 15 98,154,216 (GRCm39) missense unknown
R6241:H1f7 UTSW 15 98,154,652 (GRCm39) missense unknown
R6489:H1f7 UTSW 15 98,154,888 (GRCm39) nonsense probably null
R6703:H1f7 UTSW 15 98,155,153 (GRCm39) start gained probably benign
R7131:H1f7 UTSW 15 98,154,250 (GRCm39) nonsense probably null
R7750:H1f7 UTSW 15 98,154,565 (GRCm39) missense unknown
R9454:H1f7 UTSW 15 98,154,823 (GRCm39) missense probably benign 0.01
R9461:H1f7 UTSW 15 98,155,138 (GRCm39) missense probably damaging 0.99
Z1177:H1f7 UTSW 15 98,155,128 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- CCCTCGGCTTTGAAATCTTCCAGAC -3'
(R):5'- TGCATCTACCTTTCCACCAGGACG -3'

Sequencing Primer
(F):5'- TGAAATCTTCCAGACCCTGAAGTAG -3'
(R):5'- ATCAAAGCAGGTGGCCC -3'
Posted On 2013-07-11