Mutagenetix > Incidental Mutation

Incidental Mutation 'R0635:Vmn2r77'

56603

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r77

Ensembl Gene

ENSMUSG00000090949

Gene Name

vomeronasal 2, receptor 77

Synonyms

EG546983

MMRRC Submission

038824-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.090) question?

Stock #

R0635 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

86795141-86812032 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 86811175 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Isoleucine at position 570 (F570I)

Ref Sequence

ENSEMBL: ENSMUSP00000129540 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164996]

AlphaFold

L7N2B7

Predicted Effect

probably benign
Transcript: ENSMUST00000164996
AA Change: F570I

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000129540
Gene: ENSMUSG00000090949
AA Change: F570I

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	78	467	1.4e-30	PFAM
Pfam:NCD3G	510	562	1e-20	PFAM
Pfam:7tm_3	594	830	2.6e-52	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.4%
20x: 94.9%

Validation Efficiency

99% (68/69)

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3632451O06Rik	A	G	14: 49,773,143	L369S	probably benign	Het
4932429P05Rik	T	C	X: 89,752,522		probably benign	Het
5730455P16Rik	A	T	11: 80,374,065		probably benign	Het
Adamts15	A	G	9: 30,904,770	L631P	probably damaging	Het
Adamts17	T	C	7: 66,908,605	F266L	probably damaging	Het
Adgrb1	C	A	15: 74,540,892	Q488K	possibly damaging	Het
Cep290	A	G	10: 100,492,676	D109G	probably damaging	Het
Chil5	A	T	3: 106,017,203	Y229N	possibly damaging	Het
Cntnap1	A	G	11: 101,183,459	T742A	probably benign	Het
Col6a3	A	T	1: 90,808,086		probably null	Het
Col6a5	G	A	9: 105,928,606	P1034S	unknown	Het
Daxx	T	A	17: 33,912,644	D442E	probably benign	Het
Dmxl1	T	C	18: 49,851,423		probably benign	Het
Dnah11	A	G	12: 118,007,996	F2942S	probably damaging	Het
Fam71a	T	C	1: 191,163,727	T240A	probably benign	Het
Glg1	A	G	8: 111,163,764		probably benign	Het
Gm10272	G	A	10: 77,706,701		probably benign	Het
Gm17333	AAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA	AAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA	16: 77,852,878		noncoding transcript	Het
Haao	A	G	17: 83,838,574	F83S	probably damaging	Het
Hdgfl2	T	A	17: 56,096,057	L177Q	probably damaging	Het
Hrh1	T	C	6: 114,480,145	V129A	probably damaging	Het
Ift43	T	A	12: 86,085,081		probably benign	Het
Il21r	T	C	7: 125,632,506	Y369H	probably damaging	Het
Il2ra	C	T	2: 11,680,366	T171M	probably benign	Het
Lao1	C	T	4: 118,968,296	R438C	probably benign	Het
Lrrcc1	G	A	3: 14,559,228	S350N	probably benign	Het
Mageb5	T	A	X: 91,779,993	Y260F	probably benign	Het
March5	A	T	19: 37,220,408	I159F	possibly damaging	Het
Mgat4a	G	A	1: 37,452,294	A282V	probably benign	Het
Mipep	G	A	14: 60,829,390	V420I	probably damaging	Het
Morc2b	A	T	17: 33,137,687	F370L	possibly damaging	Het
Mt1	A	T	8: 94,179,821		probably null	Het
Ncapd2	A	G	6: 125,173,036	V943A	probably benign	Het
Nkd2	T	C	13: 73,826,894	D58G	probably benign	Het
Nol8	C	G	13: 49,676,758	S1106C	probably benign	Het
Nrm	C	A	17: 35,864,264	Y61*	probably null	Het
Nusap1	A	G	2: 119,627,667	T95A	probably damaging	Het
Ocln	T	A	13: 100,506,236	Q197L	probably damaging	Het
Olfr495	T	A	7: 108,395,764	F215I	probably benign	Het
Oxtr	A	G	6: 112,489,200	Y200H	probably damaging	Het
Paip2b	T	C	6: 83,809,909	E115G	possibly damaging	Het
Pcm1	T	A	8: 41,267,179		probably benign	Het
Pcnt	T	C	10: 76,404,585	D1205G	probably damaging	Het
Phka1	G	A	X: 102,621,400	R186C	probably damaging	Het
Pik3cb	A	G	9: 99,064,218		probably benign	Het
Pik3r1	C	T	13: 101,757,418	R81K	probably benign	Het
Ppa1	A	G	10: 61,665,440	D162G	probably benign	Het
Ppa1	A	G	10: 61,666,970	R191G	probably damaging	Het
Prss22	T	A	17: 23,996,688	T87S	probably benign	Het
Rgr	T	A	14: 37,038,947	R218*	probably null	Het
Rreb1	A	T	13: 37,941,564	Q1282L	possibly damaging	Het
Scel	T	A	14: 103,583,139		probably null	Het
Sema6b	A	G	17: 56,129,971		probably null	Het
Slc4a1	T	C	11: 102,352,672	E711G	possibly damaging	Het
Snx19	C	A	9: 30,428,810	L415M	probably damaging	Het
Snx19	T	G	9: 30,428,811	L415R	probably damaging	Het
Specc1	G	A	11: 62,118,903	R495Q	probably damaging	Het
Tead1	T	C	7: 112,891,706		probably benign	Het
Timm10b	A	C	7: 105,640,688		probably benign	Het
Ubxn7	T	A	16: 32,367,417		probably benign	Het
Vmn2r116	T	A	17: 23,386,887	Y258N	possibly damaging	Het
Vmn2r98	T	C	17: 19,080,497	V587A	probably benign	Het
Zfp398	T	C	6: 47,863,140	I101T	probably damaging	Het
Zfp808	T	A	13: 62,172,419	H487Q	probably damaging	Het

Other mutations in Vmn2r77

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00954:Vmn2r77	APN	7	86800767	missense	probably benign	0.06
IGL01105:Vmn2r77	APN	7	86811664	missense	probably damaging	0.99
IGL01367:Vmn2r77	APN	7	86811916	missense	probably damaging	0.98
IGL01634:Vmn2r77	APN	7	86811649	missense	probably benign
IGL01805:Vmn2r77	APN	7	86811187	missense	probably benign	0.18
IGL01868:Vmn2r77	APN	7	86803016	missense	probably benign	0.00
IGL01980:Vmn2r77	APN	7	86801470	missense	probably benign	0.14
IGL02055:Vmn2r77	APN	7	86801555	missense	probably benign	0.00
IGL02066:Vmn2r77	APN	7	86803628	nonsense	probably null
IGL02185:Vmn2r77	APN	7	86795152	missense	unknown
IGL02200:Vmn2r77	APN	7	86801979	missense	probably benign	0.04
IGL02336:Vmn2r77	APN	7	86802016	missense	probably damaging	0.99
IGL02445:Vmn2r77	APN	7	86803640	nonsense	probably null
IGL02557:Vmn2r77	APN	7	86795134	unclassified	probably benign
IGL02659:Vmn2r77	APN	7	86800771	missense	probably benign	0.32
IGL02978:Vmn2r77	APN	7	86811347	missense	probably benign
IGL03180:Vmn2r77	APN	7	86801635	missense	possibly damaging	0.85
IGL03255:Vmn2r77	APN	7	86811923	missense	probably benign	0.04
IGL03273:Vmn2r77	APN	7	86811286	missense	probably damaging	0.99
R0046:Vmn2r77	UTSW	7	86801938	missense	possibly damaging	0.73
R0047:Vmn2r77	UTSW	7	86811650	missense	probably benign	0.01
R0066:Vmn2r77	UTSW	7	86800756	missense	probably benign	0.17
R0066:Vmn2r77	UTSW	7	86800756	missense	probably benign	0.17
R0389:Vmn2r77	UTSW	7	86801494	missense	probably benign	0.29
R0689:Vmn2r77	UTSW	7	86811664	missense	probably damaging	0.99
R0827:Vmn2r77	UTSW	7	86802016	missense	probably damaging	1.00
R1167:Vmn2r77	UTSW	7	86801746	missense	probably benign	0.02
R1228:Vmn2r77	UTSW	7	86801034	critical splice donor site	probably null
R1353:Vmn2r77	UTSW	7	86802186	missense	probably benign	0.29
R1392:Vmn2r77	UTSW	7	86801622	missense	probably benign	0.00
R1392:Vmn2r77	UTSW	7	86801622	missense	probably benign	0.00
R1613:Vmn2r77	UTSW	7	86811148	missense	probably damaging	1.00
R1654:Vmn2r77	UTSW	7	86811915	missense	probably damaging	1.00
R1742:Vmn2r77	UTSW	7	86795335	missense	probably benign	0.35
R1827:Vmn2r77	UTSW	7	86801613	missense	probably damaging	0.99
R1911:Vmn2r77	UTSW	7	86811793	missense	probably damaging	1.00
R1974:Vmn2r77	UTSW	7	86800756	missense	probably benign	0.17
R2008:Vmn2r77	UTSW	7	86801713	missense	probably benign	0.31
R2093:Vmn2r77	UTSW	7	86801494	missense	probably benign	0.29
R2143:Vmn2r77	UTSW	7	86811944	missense	probably damaging	1.00
R2269:Vmn2r77	UTSW	7	86811689	missense	probably benign	0.03
R2972:Vmn2r77	UTSW	7	86803685	missense	probably benign	0.01
R2974:Vmn2r77	UTSW	7	86803685	missense	probably benign	0.01
R3037:Vmn2r77	UTSW	7	86800983	missense	probably benign
R3694:Vmn2r77	UTSW	7	86800836	missense	probably damaging	1.00
R3695:Vmn2r77	UTSW	7	86800836	missense	probably damaging	1.00
R3805:Vmn2r77	UTSW	7	86795160	nonsense	probably null
R3870:Vmn2r77	UTSW	7	86811842	missense	probably damaging	1.00
R4732:Vmn2r77	UTSW	7	86800987	missense	probably benign	0.00
R4733:Vmn2r77	UTSW	7	86800987	missense	probably benign	0.00
R5009:Vmn2r77	UTSW	7	86801807	missense	possibly damaging	0.82
R5201:Vmn2r77	UTSW	7	86811638	missense	probably damaging	0.98
R5218:Vmn2r77	UTSW	7	86802133	missense	probably damaging	0.98
R5469:Vmn2r77	UTSW	7	86802063	missense	probably benign	0.01
R5673:Vmn2r77	UTSW	7	86812006	missense	probably benign	0.05
R5771:Vmn2r77	UTSW	7	86812027	missense	probably benign	0.06
R5832:Vmn2r77	UTSW	7	86811462	nonsense	probably null
R5899:Vmn2r77	UTSW	7	86811716	missense	probably damaging	1.00
R6151:Vmn2r77	UTSW	7	86801670	missense	probably benign	0.00
R6182:Vmn2r77	UTSW	7	86811749	missense	probably damaging	1.00
R6326:Vmn2r77	UTSW	7	86801823	missense	probably benign
R6419:Vmn2r77	UTSW	7	86811559	missense	probably damaging	0.99
R6549:Vmn2r77	UTSW	7	86800857	missense	probably benign	0.06
R6874:Vmn2r77	UTSW	7	86802078	missense	probably benign	0.00
R6972:Vmn2r77	UTSW	7	86802994	missense	probably damaging	1.00
R7056:Vmn2r77	UTSW	7	86801815	missense	probably benign	0.06
R7185:Vmn2r77	UTSW	7	86801827	missense	probably benign	0.00
R7261:Vmn2r77	UTSW	7	86811310	nonsense	probably null
R7298:Vmn2r77	UTSW	7	86800771	missense	probably benign	0.00
R7662:Vmn2r77	UTSW	7	86811284	nonsense	probably null
R8182:Vmn2r77	UTSW	7	86811593	missense	probably damaging	1.00
R8327:Vmn2r77	UTSW	7	86801472	missense	probably benign	0.08
R8387:Vmn2r77	UTSW	7	86801739	missense	probably benign	0.00
R8825:Vmn2r77	UTSW	7	86803647	missense	probably benign
R8898:Vmn2r77	UTSW	7	86795222	missense	probably damaging	1.00
R8973:Vmn2r77	UTSW	7	86802942	missense	possibly damaging	0.93
R9258:Vmn2r77	UTSW	7	86803094	missense	possibly damaging	0.88
R9338:Vmn2r77	UTSW	7	86811786	missense	probably damaging	1.00
R9358:Vmn2r77	UTSW	7	86803028	missense	probably benign	0.00
R9377:Vmn2r77	UTSW	7	86795234	missense	probably benign	0.05
R9404:Vmn2r77	UTSW	7	86802039	missense	probably benign
R9673:Vmn2r77	UTSW	7	86800963	missense	possibly damaging	0.75
R9679:Vmn2r77	UTSW	7	86811533	missense	probably benign	0.07

Predicted Primers

PCR Primer
(F):5'- TTGCTGACAAATTGCCCTCTAATGTCT -3'
(R):5'- AGGATGCAGCTAACCATAGTGGGAT -3'

Sequencing Primer
(F):5'- GCTTGCATAAAGAATGGAAGCATTTC -3'
(R):5'- GCTAACCATAGTGGGATAACCAATG -3'

Posted On

2013-07-11