|Institutional Source||Beutler Lab|
|Gene Name||REV1, DNA directed polymerase|
|Synonyms||REV1, Rev1l, 1110027I23Rik|
|Is this an essential gene?||Probably essential (E-score: 0.927)|
|Stock #||R7298 (G1)|
|Chromosomal Location||38052786-38129801 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to C at 38053104 bp|
|Amino Acid Change||Threonine to Alanine at position 1245 (T1245A)|
|Ref Sequence||ENSEMBL: ENSMUSP00000027251 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000027251] [ENSMUST00000027252]|
|Predicted Effect||probably damaging
AA Change: T1245A
PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
AA Change: T1245A
|Predicted Effect||probably benign
|Coding Region Coverage||
|Validation Efficiency||96% (52/54)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. [provided by RefSeq, Mar 2016]
PHENOTYPE: Mice homozygous for a knock-in allele exhibit abnormal somatic hypermutation frequency of the Ig gene. Mice homozygous for a knock-out allele exhibit background-sensitive prenatal lethality and abnormal somatic hypermutation frequency. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Rev1||
(F):5'- GCAATACTGACAAATTTGGCAC -3'
(R):5'- GGCCTTTAAACTCAGTGGCTG -3'
(F):5'- CAGAGCATCACTATCATGC -3'
(R):5'- TTCAGGTTGATGCAGCAG -3'