Mutagenetix > Incidental Mutation

Incidental Mutation 'R7301:Rasl2-9'

566975

Institutional Source

Beutler Lab

Gene Symbol

Rasl2-9

Ensembl Gene

ENSMUSG00000083649

Gene Name

RAS-like, family 2, locus 9

Synonyms

Rasl2-9-ps, Ran/M2

MMRRC Submission

045405-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.905) question?

Stock #

R7301 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

5124938-5125950 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 5125740 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Arginine at position 64 (W64R)

Ref Sequence

ENSEMBL: ENSMUSP00000129559 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000147835]

AlphaFold

Q61820

Predicted Effect

probably damaging
Transcript: ENSMUST00000147835
AA Change: W64R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000129559
Gene: ENSMUSG00000083649
AA Change: W64R

Domain	Start	End	E-Value	Type
RAN	16	216	1.25e-161	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 72 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acsm3	G	A	7: 119,777,085 (GRCm38)	S345N	possibly damaging	Het
Agk	A	T	6: 40,329,517 (GRCm38)	T7S	possibly damaging	Het
Ankrd26	T	C	6: 118,511,663 (GRCm38)	E1345G	possibly damaging	Het
Atp1a3	T	A	7: 24,990,515 (GRCm38)	Y493F	probably benign	Het
Atxn2l	G	T	7: 126,494,211 (GRCm38)	Y791*	probably null	Het
Cacng8	C	A	7: 3,415,421 (GRCm38)	T363K	probably benign	Het
Camkmt	A	G	17: 85,431,493 (GRCm38)	T216A	probably benign	Het
Cd2ap	G	A	17: 42,830,013 (GRCm38)	R212*	probably null	Het
Cnppd1	A	G	1: 75,136,424 (GRCm38)	L400P	probably damaging	Het
Csmd2	C	A	4: 128,528,262 (GRCm38)	D2797E		Het
Ddx24	A	G	12: 103,419,450 (GRCm38)	M298T	possibly damaging	Het
Dpyd	T	C	3: 118,899,284 (GRCm38)	V359A	possibly damaging	Het
Dscam	T	C	16: 97,056,532 (GRCm38)	T93A	probably benign	Het
Eif2b3	T	A	4: 117,052,822 (GRCm38)	S185T	probably benign	Het
Entpd2	T	A	2: 25,400,909 (GRCm38)	I475N	possibly damaging	Het
Ercc2	C	A	7: 19,394,135 (GRCm38)	Q715K	probably benign	Het
Fam122a	T	A	19: 24,477,346 (GRCm38)	E4V	probably damaging	Het
Fam122a	T	A	19: 24,477,124 (GRCm38)	H78L	probably benign	Het
Fam186b	T	C	15: 99,278,748 (GRCm38)	R754G	probably benign	Het
Fcgbp	T	A	7: 28,093,436 (GRCm38)	V955E	possibly damaging	Het
Frrs1	T	C	3: 116,895,563 (GRCm38)	V361A	possibly damaging	Het
Gabrr2	T	A	4: 33,095,284 (GRCm38)	M391K	probably benign	Het
Gm12394	T	C	4: 42,792,923 (GRCm38)	N403S	possibly damaging	Het
Gm3409	T	A	5: 146,539,547 (GRCm38)	D169E	probably benign	Het
Gm4779	TCGGGGCCGGGGCCGGGGCCG	TCGGGGCCGGGGCCGGGGCCGGGGCCG	X: 101,794,171 (GRCm38)		probably benign	Het
Greb1l	C	G	18: 10,544,970 (GRCm38)	Q1433E	probably damaging	Het
Hal	T	C	10: 93,492,561 (GRCm38)	V233A	probably benign	Het
Ighv1-58	A	T	12: 115,312,295 (GRCm38)	N74K	probably benign	Het
Il12rb1	A	G	8: 70,813,699 (GRCm38)	I229M	possibly damaging	Het
Il17rd	T	C	14: 27,076,391 (GRCm38)	I56T	possibly damaging	Het
Itpr1	T	C	6: 108,542,024 (GRCm38)	V2708A	possibly damaging	Het
Klhl38	G	A	15: 58,322,980 (GRCm38)	R118W	probably damaging	Het
Lmf2	C	A	15: 89,355,530 (GRCm38)		probably benign	Het
Lrrc3b	T	C	14: 15,357,934 (GRCm38)	Y224C	probably damaging	Het
Med1	A	C	11: 98,152,808 (GRCm38)	F599C	probably benign	Het
Mrgprb4	A	G	7: 48,198,758 (GRCm38)	S141P	probably damaging	Het
Mst1r	G	T	9: 107,914,790 (GRCm38)	A842S	possibly damaging	Het
Myo3a	T	C	2: 22,544,466 (GRCm38)		probably null	Het
Nos1	A	T	5: 117,867,905 (GRCm38)	D230V	possibly damaging	Het
Nppb	T	C	4: 147,986,323 (GRCm38)	S52P	probably benign	Het
Nqo1	A	G	8: 107,392,648 (GRCm38)	I99T	probably damaging	Het
Olfr346	T	A	2: 36,688,011 (GRCm38)	M3K	probably benign	Het
Olfr769	T	C	10: 129,111,699 (GRCm38)	H242R	probably damaging	Het
Pcdha3	G	A	18: 36,946,924 (GRCm38)	E240K	possibly damaging	Het
Plpp7	T	G	2: 32,096,055 (GRCm38)	F82V	probably benign	Het
Podxl	G	T	6: 31,524,436 (GRCm38)	P395T	probably damaging	Het
Prr5l	T	A	2: 101,717,286 (GRCm38)	D298V	probably damaging	Het
Rad9b	A	G	5: 122,352,614 (GRCm38)	V13A	possibly damaging	Het
Rilp	G	T	11: 75,510,116 (GRCm38)		probably benign	Het
Ripor2	T	C	13: 24,725,001 (GRCm38)	I1034T	possibly damaging	Het
Rtn4ip1	T	C	10: 43,936,020 (GRCm38)	Y338H	probably damaging	Het
Shisa5	G	T	9: 109,054,884 (GRCm38)		probably benign	Het
Slc27a4	C	T	2: 29,812,932 (GRCm38)	T591I	probably null	Het
Snx24	G	T	18: 53,340,172 (GRCm38)	V63F	probably damaging	Het
Sptbn1	A	T	11: 30,117,798 (GRCm38)	Y1805*	probably null	Het
Svep1	G	A	4: 58,046,587 (GRCm38)	Q3515*	probably null	Het
Synpo2	A	C	3: 123,114,053 (GRCm38)	M538R	probably benign	Het
Tfap2a	T	C	13: 40,721,308 (GRCm38)	K276E	probably damaging	Het
Tmem158	C	A	9: 123,260,301 (GRCm38)	S82I	probably damaging	Het
Tmtc3	G	T	10: 100,447,474 (GRCm38)	H740N	not run	Het
Top3a	A	T	11: 60,748,148 (GRCm38)	F559I	probably damaging	Het
Tysnd1	C	A	10: 61,696,549 (GRCm38)	P327T	possibly damaging	Het
Ulk4	T	A	9: 121,145,059 (GRCm38)	D969V	probably benign	Het
Vcan	T	A	13: 89,705,266 (GRCm38)	Y525F	probably benign	Het
Vmn1r127	A	G	7: 21,319,053 (GRCm38)	F270S	probably benign	Het
Vmn1r204	G	A	13: 22,556,805 (GRCm38)	S202N	probably damaging	Het
Vmn2r107	A	G	17: 20,345,616 (GRCm38)	I64M	probably benign	Het
Zfp280b	C	G	10: 76,038,703 (GRCm38)	Q139E	probably damaging	Het
Zfp322a	C	T	13: 23,357,144 (GRCm38)	G143S	probably benign	Het
Zfp322a	C	A	13: 23,357,143 (GRCm38)	G143V	probably damaging	Het
Zfyve26	A	T	12: 79,282,984 (GRCm38)	V476D	probably benign	Het
Zkscan6	A	T	11: 65,828,225 (GRCm38)	H357L	probably benign	Het

Other mutations in Rasl2-9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02110:Rasl2-9	APN	7	5,125,347 (GRCm38)	missense	probably benign	0.00
R1500:Rasl2-9	UTSW	7	5,125,442 (GRCm38)	nonsense	probably null
R4566:Rasl2-9	UTSW	7	5,125,375 (GRCm38)	frame shift	probably null
R4567:Rasl2-9	UTSW	7	5,125,375 (GRCm38)	frame shift	probably null
R4568:Rasl2-9	UTSW	7	5,125,375 (GRCm38)	frame shift	probably null
R4745:Rasl2-9	UTSW	7	5,125,703 (GRCm38)	missense	possibly damaging	0.90
R8025:Rasl2-9	UTSW	7	5,125,482 (GRCm38)	missense	probably damaging	1.00
R8281:Rasl2-9	UTSW	7	5,125,352 (GRCm38)	nonsense	probably null
R9272:Rasl2-9	UTSW	7	5,125,449 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCTTTCGGTGGAAGAGAATAGG -3'
(R):5'- AGGTCCAGTTCAAGGTCGTC -3'

Sequencing Primer
(F):5'- CACTTTGTTGCCACACAATACAATGG -3'
(R):5'- CAGTTCAAGGTCGTCCTGGTG -3'

Posted On

2019-06-26