Incidental Mutation 'R7301:Rasl2-9'
ID 566975
Institutional Source Beutler Lab
Gene Symbol Rasl2-9
Ensembl Gene ENSMUSG00000083649
Gene Name RAS-like, family 2, locus 9
Synonyms Rasl2-9-ps, Ran/M2
MMRRC Submission 045405-MU
Accession Numbers
Essential gene? Probably essential (E-score: 0.905) question?
Stock # R7301 (G1)
Quality Score 225.009
Status Not validated
Chromosome 7
Chromosomal Location 5124938-5125950 bp(-) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) A to G at 5125740 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Tryptophan to Arginine at position 64 (W64R)
Ref Sequence ENSEMBL: ENSMUSP00000129559 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000147835]
AlphaFold Q61820
Predicted Effect probably damaging
Transcript: ENSMUST00000147835
AA Change: W64R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000129559
Gene: ENSMUSG00000083649
AA Change: W64R

RAN 16 216 1.25e-161 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.7%
  • 20x: 99.0%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 72 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acsm3 G A 7: 119,777,085 (GRCm38) S345N possibly damaging Het
Agk A T 6: 40,329,517 (GRCm38) T7S possibly damaging Het
Ankrd26 T C 6: 118,511,663 (GRCm38) E1345G possibly damaging Het
Atp1a3 T A 7: 24,990,515 (GRCm38) Y493F probably benign Het
Atxn2l G T 7: 126,494,211 (GRCm38) Y791* probably null Het
Cacng8 C A 7: 3,415,421 (GRCm38) T363K probably benign Het
Camkmt A G 17: 85,431,493 (GRCm38) T216A probably benign Het
Cd2ap G A 17: 42,830,013 (GRCm38) R212* probably null Het
Cnppd1 A G 1: 75,136,424 (GRCm38) L400P probably damaging Het
Csmd2 C A 4: 128,528,262 (GRCm38) D2797E Het
Ddx24 A G 12: 103,419,450 (GRCm38) M298T possibly damaging Het
Dpyd T C 3: 118,899,284 (GRCm38) V359A possibly damaging Het
Dscam T C 16: 97,056,532 (GRCm38) T93A probably benign Het
Eif2b3 T A 4: 117,052,822 (GRCm38) S185T probably benign Het
Entpd2 T A 2: 25,400,909 (GRCm38) I475N possibly damaging Het
Ercc2 C A 7: 19,394,135 (GRCm38) Q715K probably benign Het
Fam122a T A 19: 24,477,346 (GRCm38) E4V probably damaging Het
Fam122a T A 19: 24,477,124 (GRCm38) H78L probably benign Het
Fam186b T C 15: 99,278,748 (GRCm38) R754G probably benign Het
Fcgbp T A 7: 28,093,436 (GRCm38) V955E possibly damaging Het
Frrs1 T C 3: 116,895,563 (GRCm38) V361A possibly damaging Het
Gabrr2 T A 4: 33,095,284 (GRCm38) M391K probably benign Het
Gm12394 T C 4: 42,792,923 (GRCm38) N403S possibly damaging Het
Gm3409 T A 5: 146,539,547 (GRCm38) D169E probably benign Het
Greb1l C G 18: 10,544,970 (GRCm38) Q1433E probably damaging Het
Hal T C 10: 93,492,561 (GRCm38) V233A probably benign Het
Ighv1-58 A T 12: 115,312,295 (GRCm38) N74K probably benign Het
Il12rb1 A G 8: 70,813,699 (GRCm38) I229M possibly damaging Het
Il17rd T C 14: 27,076,391 (GRCm38) I56T possibly damaging Het
Itpr1 T C 6: 108,542,024 (GRCm38) V2708A possibly damaging Het
Klhl38 G A 15: 58,322,980 (GRCm38) R118W probably damaging Het
Lmf2 C A 15: 89,355,530 (GRCm38) probably benign Het
Lrrc3b T C 14: 15,357,934 (GRCm38) Y224C probably damaging Het
Med1 A C 11: 98,152,808 (GRCm38) F599C probably benign Het
Mrgprb4 A G 7: 48,198,758 (GRCm38) S141P probably damaging Het
Mst1r G T 9: 107,914,790 (GRCm38) A842S possibly damaging Het
Myo3a T C 2: 22,544,466 (GRCm38) probably null Het
Nos1 A T 5: 117,867,905 (GRCm38) D230V possibly damaging Het
Nppb T C 4: 147,986,323 (GRCm38) S52P probably benign Het
Nqo1 A G 8: 107,392,648 (GRCm38) I99T probably damaging Het
Olfr346 T A 2: 36,688,011 (GRCm38) M3K probably benign Het
Olfr769 T C 10: 129,111,699 (GRCm38) H242R probably damaging Het
Pcdha3 G A 18: 36,946,924 (GRCm38) E240K possibly damaging Het
Plpp7 T G 2: 32,096,055 (GRCm38) F82V probably benign Het
Podxl G T 6: 31,524,436 (GRCm38) P395T probably damaging Het
Prr5l T A 2: 101,717,286 (GRCm38) D298V probably damaging Het
Rad9b A G 5: 122,352,614 (GRCm38) V13A possibly damaging Het
Rilp G T 11: 75,510,116 (GRCm38) probably benign Het
Ripor2 T C 13: 24,725,001 (GRCm38) I1034T possibly damaging Het
Rtn4ip1 T C 10: 43,936,020 (GRCm38) Y338H probably damaging Het
Shisa5 G T 9: 109,054,884 (GRCm38) probably benign Het
Slc27a4 C T 2: 29,812,932 (GRCm38) T591I probably null Het
Snx24 G T 18: 53,340,172 (GRCm38) V63F probably damaging Het
Sptbn1 A T 11: 30,117,798 (GRCm38) Y1805* probably null Het
Svep1 G A 4: 58,046,587 (GRCm38) Q3515* probably null Het
Synpo2 A C 3: 123,114,053 (GRCm38) M538R probably benign Het
Tfap2a T C 13: 40,721,308 (GRCm38) K276E probably damaging Het
Tmem158 C A 9: 123,260,301 (GRCm38) S82I probably damaging Het
Tmtc3 G T 10: 100,447,474 (GRCm38) H740N not run Het
Top3a A T 11: 60,748,148 (GRCm38) F559I probably damaging Het
Tysnd1 C A 10: 61,696,549 (GRCm38) P327T possibly damaging Het
Ulk4 T A 9: 121,145,059 (GRCm38) D969V probably benign Het
Vcan T A 13: 89,705,266 (GRCm38) Y525F probably benign Het
Vmn1r127 A G 7: 21,319,053 (GRCm38) F270S probably benign Het
Vmn1r204 G A 13: 22,556,805 (GRCm38) S202N probably damaging Het
Vmn2r107 A G 17: 20,345,616 (GRCm38) I64M probably benign Het
Zfp280b C G 10: 76,038,703 (GRCm38) Q139E probably damaging Het
Zfp322a C T 13: 23,357,144 (GRCm38) G143S probably benign Het
Zfp322a C A 13: 23,357,143 (GRCm38) G143V probably damaging Het
Zfyve26 A T 12: 79,282,984 (GRCm38) V476D probably benign Het
Zkscan6 A T 11: 65,828,225 (GRCm38) H357L probably benign Het
Other mutations in Rasl2-9
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02110:Rasl2-9 APN 7 5,125,347 (GRCm38) missense probably benign 0.00
R1500:Rasl2-9 UTSW 7 5,125,442 (GRCm38) nonsense probably null
R4566:Rasl2-9 UTSW 7 5,125,375 (GRCm38) frame shift probably null
R4567:Rasl2-9 UTSW 7 5,125,375 (GRCm38) frame shift probably null
R4568:Rasl2-9 UTSW 7 5,125,375 (GRCm38) frame shift probably null
R4745:Rasl2-9 UTSW 7 5,125,703 (GRCm38) missense possibly damaging 0.90
R8025:Rasl2-9 UTSW 7 5,125,482 (GRCm38) missense probably damaging 1.00
R8281:Rasl2-9 UTSW 7 5,125,352 (GRCm38) nonsense probably null
R9272:Rasl2-9 UTSW 7 5,125,449 (GRCm38) missense probably damaging 1.00
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2019-06-26