|Institutional Source||Beutler Lab|
|Gene Name||transmembrane protein 158|
|Is this an essential gene?||Non essential (E-score: 0.000)|
|Stock #||R7301 (G1)|
|Chromosomal Location||123259053-123260764 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||C to A at 123260301 bp|
|Amino Acid Change||Serine to Isoleucine at position 82 (S82I)|
|Ref Sequence||ENSEMBL: ENSMUSP00000069161 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000068140]|
|Predicted Effect||probably damaging
AA Change: S82I
PolyPhen 2 Score 0.959 (Sensitivity: 0.78; Specificity: 0.95)
AA Change: S82I
|Coding Region Coverage||
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Constitutive activation of the Ras pathway triggers an irreversible proliferation arrest reminiscent of replicative senescence. Transcription of this gene is upregulated in response to activation of the Ras pathway, but not under other conditions that induce senescence. The encoded protein is similar to a rat cell surface receptor proposed to function in a neuronal survival pathway. An allelic polymorphism in this gene results in both functional and non-functional (frameshifted) alleles; the reference genome represents the functional allele. [provided by RefSeq, Jul 2015]
PHENOTYPE: Mice homozygous for a knock-out allele are viable, fertile and developmentally normal, and exhibit a normal lifespan and no predisposition to spontaneous or chemically-induced tumors. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Tmem158||
(F):5'- CAGCAGAAGTGACGAGGCTC -3'
(R):5'- AATACACTGGCAGAGTTCCG -3'
(F):5'- TCGCCCTGCAGCCACAG -3'
(R):5'- TGGCAGAGTTCCGCCTCG -3'