Mutagenetix > Incidental Mutation

Incidental Mutation 'R7301:Vcan'

567008

Institutional Source

Beutler Lab

Gene Symbol

Vcan

Ensembl Gene

ENSMUSG00000021614

Gene Name

versican

Synonyms

PG-M, hdf, DPEAAE, heart defect, Cspg2, 5430420N07Rik

MMRRC Submission

045405-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7301 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

89655312-89742509 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 89705266 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Phenylalanine at position 525 (Y525F)

Ref Sequence

ENSEMBL: ENSMUSP00000105173 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109543] [ENSMUST00000109544] [ENSMUST00000109546] [ENSMUST00000159910]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000109543

SMART Domains

Protein: ENSMUSP00000105170
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
EGF	351	384	2.72e-7	SMART
EGF_CA	386	422	1.16e-10	SMART
CLECT	428	549	3.08e-34	SMART
CCP	555	611	1.04e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000109544
AA Change: Y525F

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000105171
Gene: ENSMUSG00000021614
AA Change: Y525F

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
EGF	1311	1344	2.72e-7	SMART
EGF_CA	1346	1382	1.16e-10	SMART
CLECT	1388	1509	3.08e-34	SMART
CCP	1515	1571	1.04e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000109546
AA Change: Y525F

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000105173
Gene: ENSMUSG00000021614
AA Change: Y525F

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
low complexity region	1322	1333	N/A	INTRINSIC
low complexity region	1546	1569	N/A	INTRINSIC
low complexity region	1837	1852	N/A	INTRINSIC
low complexity region	2013	2026	N/A	INTRINSIC
low complexity region	2354	2367	N/A	INTRINSIC
low complexity region	2468	2482	N/A	INTRINSIC
low complexity region	2719	2728	N/A	INTRINSIC
EGF	3050	3083	2.72e-7	SMART
EGF_CA	3085	3121	1.16e-10	SMART
CLECT	3127	3248	3.08e-34	SMART
CCP	3254	3310	1.04e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000159910

SMART Domains

Protein: ENSMUSP00000125446
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	362	373	N/A	INTRINSIC
low complexity region	586	609	N/A	INTRINSIC
low complexity region	877	892	N/A	INTRINSIC
low complexity region	1053	1066	N/A	INTRINSIC
low complexity region	1394	1407	N/A	INTRINSIC
low complexity region	1508	1522	N/A	INTRINSIC
low complexity region	1759	1768	N/A	INTRINSIC
EGF	2090	2123	2.72e-7	SMART
EGF_CA	2125	2161	1.16e-10	SMART
CLECT	2167	2288	3.08e-34	SMART
CCP	2294	2350	1.04e-8	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
PHENOTYPE: Homozygotes for an insertional mutation exhibit anterior-posterior segmental defects of the heart, lack endocardial cushions of the conus and atrioventricular region, and die and around embryonic day 10.5. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 72 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acsm3	G	A	7: 119,777,085	S345N	possibly damaging	Het
Agk	A	T	6: 40,329,517	T7S	possibly damaging	Het
Ankrd26	T	C	6: 118,511,663	E1345G	possibly damaging	Het
Atp1a3	T	A	7: 24,990,515	Y493F	probably benign	Het
Atxn2l	G	T	7: 126,494,211	Y791*	probably null	Het
Cacng8	C	A	7: 3,415,421	T363K	probably benign	Het
Camkmt	A	G	17: 85,431,493	T216A	probably benign	Het
Cd2ap	G	A	17: 42,830,013	R212*	probably null	Het
Cnppd1	A	G	1: 75,136,424	L400P	probably damaging	Het
Csmd2	C	A	4: 128,528,262	D2797E		Het
Ddx24	A	G	12: 103,419,450	M298T	possibly damaging	Het
Dpyd	T	C	3: 118,899,284	V359A	possibly damaging	Het
Dscam	T	C	16: 97,056,532	T93A	probably benign	Het
Eif2b3	T	A	4: 117,052,822	S185T	probably benign	Het
Entpd2	T	A	2: 25,400,909	I475N	possibly damaging	Het
Ercc2	C	A	7: 19,394,135	Q715K	probably benign	Het
Fam122a	T	A	19: 24,477,124	H78L	probably benign	Het
Fam122a	T	A	19: 24,477,346	E4V	probably damaging	Het
Fam186b	T	C	15: 99,278,748	R754G	probably benign	Het
Fcgbp	T	A	7: 28,093,436	V955E	possibly damaging	Het
Frrs1	T	C	3: 116,895,563	V361A	possibly damaging	Het
Gabrr2	T	A	4: 33,095,284	M391K	probably benign	Het
Gm12394	T	C	4: 42,792,923	N403S	possibly damaging	Het
Gm3409	T	A	5: 146,539,547	D169E	probably benign	Het
Gm4779	TCGGGGCCGGGGCCGGGGCCG	TCGGGGCCGGGGCCGGGGCCGGGGCCG	X: 101,794,171		probably benign	Het
Greb1l	C	G	18: 10,544,970	Q1433E	probably damaging	Het
Hal	T	C	10: 93,492,561	V233A	probably benign	Het
Ighv1-58	A	T	12: 115,312,295	N74K	probably benign	Het
Il12rb1	A	G	8: 70,813,699	I229M	possibly damaging	Het
Il17rd	T	C	14: 27,076,391	I56T	possibly damaging	Het
Itpr1	T	C	6: 108,542,024	V2708A	possibly damaging	Het
Klhl38	G	A	15: 58,322,980	R118W	probably damaging	Het
Lmf2	C	A	15: 89,355,530		probably benign	Het
Lrrc3b	T	C	14: 15,357,934	Y224C	probably damaging	Het
Med1	A	C	11: 98,152,808	F599C	probably benign	Het
Mrgprb4	A	G	7: 48,198,758	S141P	probably damaging	Het
Mst1r	G	T	9: 107,914,790	A842S	possibly damaging	Het
Myo3a	T	C	2: 22,544,466		probably null	Het
Nos1	A	T	5: 117,867,905	D230V	possibly damaging	Het
Nppb	T	C	4: 147,986,323	S52P	probably benign	Het
Nqo1	A	G	8: 107,392,648	I99T	probably damaging	Het
Olfr346	T	A	2: 36,688,011	M3K	probably benign	Het
Olfr769	T	C	10: 129,111,699	H242R	probably damaging	Het
Pcdha3	G	A	18: 36,946,924	E240K	possibly damaging	Het
Plpp7	T	G	2: 32,096,055	F82V	probably benign	Het
Podxl	G	T	6: 31,524,436	P395T	probably damaging	Het
Prr5l	T	A	2: 101,717,286	D298V	probably damaging	Het
Rad9b	A	G	5: 122,352,614	V13A	possibly damaging	Het
Rasl2-9	A	G	7: 5,125,740	W64R	probably damaging	Het
Rilp	G	T	11: 75,510,116		probably benign	Het
Ripor2	T	C	13: 24,725,001	I1034T	possibly damaging	Het
Rtn4ip1	T	C	10: 43,936,020	Y338H	probably damaging	Het
Shisa5	G	T	9: 109,054,884		probably benign	Het
Slc27a4	C	T	2: 29,812,932	T591I	probably null	Het
Snx24	G	T	18: 53,340,172	V63F	probably damaging	Het
Sptbn1	A	T	11: 30,117,798	Y1805*	probably null	Het
Svep1	G	A	4: 58,046,587	Q3515*	probably null	Het
Synpo2	A	C	3: 123,114,053	M538R	probably benign	Het
Tfap2a	T	C	13: 40,721,308	K276E	probably damaging	Het
Tmem158	C	A	9: 123,260,301	S82I	probably damaging	Het
Tmtc3	G	T	10: 100,447,474	H740N	not run	Het
Top3a	A	T	11: 60,748,148	F559I	probably damaging	Het
Tysnd1	C	A	10: 61,696,549	P327T	possibly damaging	Het
Ulk4	T	A	9: 121,145,059	D969V	probably benign	Het
Vmn1r127	A	G	7: 21,319,053	F270S	probably benign	Het
Vmn1r204	G	A	13: 22,556,805	S202N	probably damaging	Het
Vmn2r107	A	G	17: 20,345,616	I64M	probably benign	Het
Zfp280b	C	G	10: 76,038,703	Q139E	probably damaging	Het
Zfp322a	C	A	13: 23,357,143	G143V	probably damaging	Het
Zfp322a	C	T	13: 23,357,144	G143S	probably benign	Het
Zfyve26	A	T	12: 79,282,984	V476D	probably benign	Het
Zkscan6	A	T	11: 65,828,225	H357L	probably benign	Het

Other mutations in Vcan

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00434:Vcan	APN	13	89704702	missense	probably damaging	1.00
IGL00502:Vcan	APN	13	89692319	missense	probably benign
IGL00504:Vcan	APN	13	89691275	missense	possibly damaging	0.70
IGL00566:Vcan	APN	13	89688979	missense	probably benign	0.01
IGL00701:Vcan	APN	13	89703726	missense	probably benign
IGL00743:Vcan	APN	13	89725306	missense	probably damaging	0.98
IGL00962:Vcan	APN	13	89662052	missense	probably damaging	1.00
IGL01085:Vcan	APN	13	89679958	missense	probably damaging	1.00
IGL01317:Vcan	APN	13	89691668	missense	probably benign	0.00
IGL01349:Vcan	APN	13	89703943	missense	probably damaging	0.98
IGL01391:Vcan	APN	13	89704169	missense	probably benign	0.19
IGL01644:Vcan	APN	13	89688675	missense	probably benign	0.13
IGL01657:Vcan	APN	13	89690586	missense	probably damaging	1.00
IGL01707:Vcan	APN	13	89689745	missense	probably damaging	1.00
IGL01764:Vcan	APN	13	89725388	missense	probably damaging	1.00
IGL01920:Vcan	APN	13	89689205	missense	probably benign	0.04
IGL01989:Vcan	APN	13	89689359	missense	possibly damaging	0.86
IGL01999:Vcan	APN	13	89684438	missense	probably damaging	1.00
IGL02083:Vcan	APN	13	89725565	missense	probably damaging	1.00
IGL02160:Vcan	APN	13	89684493	missense	probably damaging	1.00
IGL02217:Vcan	APN	13	89703077	missense	probably damaging	1.00
IGL02522:Vcan	APN	13	89704849	missense	probably benign	0.00
IGL02527:Vcan	APN	13	89690657	missense	possibly damaging	0.95
IGL02926:Vcan	APN	13	89688623	missense	probably damaging	0.98
IGL03061:Vcan	APN	13	89703275	missense	probably benign	0.25
IGL03331:Vcan	APN	13	89661932	missense	probably damaging	1.00
IGL03352:Vcan	APN	13	89705006	missense	probably benign	0.00
R0041:Vcan	UTSW	13	89661985	missense	probably damaging	1.00
R0102:Vcan	UTSW	13	89703668	missense	probably benign	0.01
R0102:Vcan	UTSW	13	89703668	missense	probably benign	0.01
R0109:Vcan	UTSW	13	89678073	critical splice donor site	probably null
R0139:Vcan	UTSW	13	89691261	missense	probably damaging	1.00
R0295:Vcan	UTSW	13	89712191	missense	probably benign	0.06
R0375:Vcan	UTSW	13	89691275	missense	probably damaging	0.99
R0379:Vcan	UTSW	13	89703546	missense	probably damaging	0.99
R0457:Vcan	UTSW	13	89703199	missense	possibly damaging	0.78
R0482:Vcan	UTSW	13	89678145	missense	probably damaging	1.00
R0485:Vcan	UTSW	13	89704660	missense	possibly damaging	0.92
R0532:Vcan	UTSW	13	89703772	missense	probably damaging	0.99
R0561:Vcan	UTSW	13	89712253	missense	probably damaging	1.00
R0561:Vcan	UTSW	13	89731464	missense	possibly damaging	0.86
R0636:Vcan	UTSW	13	89704706	missense	probably damaging	0.99
R0636:Vcan	UTSW	13	89712267	missense	probably damaging	1.00
R0680:Vcan	UTSW	13	89679822	missense	probably damaging	1.00
R0849:Vcan	UTSW	13	89704953	missense	possibly damaging	0.75
R1006:Vcan	UTSW	13	89685077	critical splice donor site	probably null
R1104:Vcan	UTSW	13	89692410	missense	probably damaging	1.00
R1118:Vcan	UTSW	13	89705663	missense	probably damaging	1.00
R1137:Vcan	UTSW	13	89704303	missense	probably damaging	1.00
R1199:Vcan	UTSW	13	89679794	splice site	probably null
R1219:Vcan	UTSW	13	89679904	missense	probably damaging	1.00
R1296:Vcan	UTSW	13	89657556	missense	probably damaging	1.00
R1332:Vcan	UTSW	13	89693055	missense	probably damaging	1.00
R1336:Vcan	UTSW	13	89693055	missense	probably damaging	1.00
R1403:Vcan	UTSW	13	89688484	missense	probably benign	0.00
R1403:Vcan	UTSW	13	89688484	missense	probably benign	0.00
R1546:Vcan	UTSW	13	89692956	missense	probably damaging	0.99
R1604:Vcan	UTSW	13	89689661	missense	probably benign	0.42
R1616:Vcan	UTSW	13	89705663	missense	probably damaging	1.00
R1636:Vcan	UTSW	13	89703667	missense	possibly damaging	0.90
R1654:Vcan	UTSW	13	89661946	missense	probably damaging	1.00
R1680:Vcan	UTSW	13	89703547	missense	probably benign	0.19
R1694:Vcan	UTSW	13	89688483	missense	probably damaging	0.98
R1712:Vcan	UTSW	13	89721775	missense	probably damaging	1.00
R1754:Vcan	UTSW	13	89704735	missense	probably benign	0.01
R1756:Vcan	UTSW	13	89691681	missense	probably benign	0.05
R1824:Vcan	UTSW	13	89705212	missense	possibly damaging	0.75
R1852:Vcan	UTSW	13	89705392	missense	probably damaging	0.99
R1868:Vcan	UTSW	13	89690871	missense	probably benign	0.12
R1920:Vcan	UTSW	13	89693015	missense	probably damaging	1.00
R1932:Vcan	UTSW	13	89705534	missense	possibly damaging	0.78
R1934:Vcan	UTSW	13	89702926	missense	probably damaging	1.00
R1942:Vcan	UTSW	13	89703424	missense	probably benign	0.01
R1964:Vcan	UTSW	13	89692742	missense	probably benign	0.02
R1970:Vcan	UTSW	13	89689038	missense	probably damaging	1.00
R2045:Vcan	UTSW	13	89690985	missense	probably benign	0.00
R2110:Vcan	UTSW	13	89693303	missense	probably damaging	1.00
R2111:Vcan	UTSW	13	89693303	missense	probably damaging	1.00
R2112:Vcan	UTSW	13	89693303	missense	probably damaging	1.00
R2136:Vcan	UTSW	13	89689737	missense	probably damaging	1.00
R2158:Vcan	UTSW	13	89703529	missense	possibly damaging	0.68
R2376:Vcan	UTSW	13	89703410	missense	possibly damaging	0.80
R2385:Vcan	UTSW	13	89689449	missense	probably damaging	1.00
R2443:Vcan	UTSW	13	89704675	missense	probably damaging	1.00
R2876:Vcan	UTSW	13	89704237	missense	probably damaging	1.00
R3607:Vcan	UTSW	13	89703301	missense	probably damaging	0.98
R4042:Vcan	UTSW	13	89692543	missense	probably benign	0.35
R4043:Vcan	UTSW	13	89692543	missense	probably benign	0.35
R4044:Vcan	UTSW	13	89692543	missense	probably benign	0.35
R4065:Vcan	UTSW	13	89679887	missense	probably damaging	1.00
R4161:Vcan	UTSW	13	89685158	missense	probably damaging	1.00
R4178:Vcan	UTSW	13	89725547	missense	probably damaging	1.00
R4290:Vcan	UTSW	13	89725486	missense	probably damaging	1.00
R4530:Vcan	UTSW	13	89704028	missense	probably damaging	0.97
R4666:Vcan	UTSW	13	89679934	missense	probably damaging	1.00
R4785:Vcan	UTSW	13	89705789	missense	probably damaging	1.00
R4870:Vcan	UTSW	13	89704739	missense	probably benign	0.01
R4973:Vcan	UTSW	13	89688842	missense	probably benign	0.30
R5037:Vcan	UTSW	13	89703977	missense	probably damaging	1.00
R5104:Vcan	UTSW	13	89657472	intron	probably benign
R5124:Vcan	UTSW	13	89725517	missense	probably damaging	1.00
R5129:Vcan	UTSW	13	89690240	missense	probably damaging	1.00
R5198:Vcan	UTSW	13	89690872	missense	probably damaging	1.00
R5240:Vcan	UTSW	13	89692532	missense	probably benign	0.08
R5254:Vcan	UTSW	13	89691600	missense	probably damaging	0.99
R5280:Vcan	UTSW	13	89690286	missense	probably benign	0.00
R5522:Vcan	UTSW	13	89691810	missense	possibly damaging	0.62
R5557:Vcan	UTSW	13	89703112	missense	possibly damaging	0.77
R5568:Vcan	UTSW	13	89688671	missense	probably damaging	1.00
R5578:Vcan	UTSW	13	89691503	missense	probably benign	0.01
R5627:Vcan	UTSW	13	89691135	frame shift	probably null
R5687:Vcan	UTSW	13	89678134	missense	probably damaging	1.00
R5752:Vcan	UTSW	13	89679950	missense	probably damaging	1.00
R5879:Vcan	UTSW	13	89703952	missense	probably damaging	0.99
R5941:Vcan	UTSW	13	89692691	missense	probably damaging	0.98
R6113:Vcan	UTSW	13	89657536	nonsense	probably null
R6135:Vcan	UTSW	13	89689926	missense	probably benign	0.36
R6252:Vcan	UTSW	13	89691220	nonsense	probably null
R6280:Vcan	UTSW	13	89725373	missense	probably damaging	1.00
R6317:Vcan	UTSW	13	89691597	missense	probably benign	0.22
R6327:Vcan	UTSW	13	89704832	missense	probably damaging	0.99
R6460:Vcan	UTSW	13	89690687	missense	possibly damaging	0.61
R6669:Vcan	UTSW	13	89704731	missense	probably benign	0.21
R6744:Vcan	UTSW	13	89705182	missense	probably damaging	1.00
R6819:Vcan	UTSW	13	89705125	missense	probably benign	0.00
R6880:Vcan	UTSW	13	89712381	missense	probably damaging	1.00
R6956:Vcan	UTSW	13	89689431	missense	probably damaging	0.99
R6971:Vcan	UTSW	13	89678133	missense	probably damaging	1.00
R6985:Vcan	UTSW	13	89679956	missense	probably damaging	1.00
R6994:Vcan	UTSW	13	89693407	missense	possibly damaging	0.94
R6997:Vcan	UTSW	13	89690618	missense	probably damaging	0.98
R7029:Vcan	UTSW	13	89690241	missense	probably damaging	1.00
R7066:Vcan	UTSW	13	89705686	missense	probably damaging	1.00
R7156:Vcan	UTSW	13	89689110	missense	possibly damaging	0.95
R7171:Vcan	UTSW	13	89725591	missense	probably damaging	1.00
R7176:Vcan	UTSW	13	89688936	missense	probably benign	0.01
R7229:Vcan	UTSW	13	89705270	missense	possibly damaging	0.87
R7250:Vcan	UTSW	13	89721686	missense	probably damaging	1.00
R7250:Vcan	UTSW	13	89731457	critical splice donor site	probably null
R7262:Vcan	UTSW	13	89705161	missense	possibly damaging	0.62
R7289:Vcan	UTSW	13	89692733	nonsense	probably null
R7299:Vcan	UTSW	13	89705266	missense	probably benign
R7425:Vcan	UTSW	13	89689832	missense	probably damaging	0.99
R7514:Vcan	UTSW	13	89704118	missense	probably damaging	0.97
R7579:Vcan	UTSW	13	89692458	missense	probably damaging	1.00
R7618:Vcan	UTSW	13	89692223	missense	probably damaging	0.99
R7655:Vcan	UTSW	13	89685114	missense	probably damaging	1.00
R7656:Vcan	UTSW	13	89685114	missense	probably damaging	1.00
R7676:Vcan	UTSW	13	89691789	missense	probably damaging	1.00
R7719:Vcan	UTSW	13	89704619	missense	probably damaging	0.98
R7753:Vcan	UTSW	13	89689323	missense	probably damaging	1.00
R7762:Vcan	UTSW	13	89692937	missense	probably damaging	1.00
R7778:Vcan	UTSW	13	89688654	missense	probably damaging	1.00
R7824:Vcan	UTSW	13	89688654	missense	probably damaging	1.00
R7995:Vcan	UTSW	13	89691858	missense	probably benign
R7998:Vcan	UTSW	13	89704327	missense	probably damaging	1.00
R8033:Vcan	UTSW	13	89704360	missense	probably benign	0.04
R8061:Vcan	UTSW	13	89657290	missense	probably benign	0.45
R8103:Vcan	UTSW	13	89657658	missense	probably damaging	1.00
R8103:Vcan	UTSW	13	89703320	nonsense	probably null
R8124:Vcan	UTSW	13	89704254	missense	possibly damaging	0.93
R8162:Vcan	UTSW	13	89704987	nonsense	probably null
R8166:Vcan	UTSW	13	89692736	missense	probably benign	0.02
R8274:Vcan	UTSW	13	89704970	missense	probably benign	0.02
R8284:Vcan	UTSW	13	89704335	missense	possibly damaging	0.68
R8417:Vcan	UTSW	13	89688743	missense	probably benign	0.19
R8696:Vcan	UTSW	13	89691098	missense	probably benign	0.00
R8738:Vcan	UTSW	13	89692320	missense	probably benign	0.17
R8792:Vcan	UTSW	13	89692111	missense	possibly damaging	0.91
R8887:Vcan	UTSW	13	89704907	missense	probably benign
R9049:Vcan	UTSW	13	89678105	missense	probably damaging	1.00
R9074:Vcan	UTSW	13	89691027	missense	possibly damaging	0.95
R9095:Vcan	UTSW	13	89704525	missense	probably benign	0.32
R9172:Vcan	UTSW	13	89679931	missense	probably damaging	1.00
R9199:Vcan	UTSW	13	89690496	nonsense	probably null
R9259:Vcan	UTSW	13	89690870	missense	probably damaging	0.99
R9455:Vcan	UTSW	13	89689333	missense	probably damaging	1.00
R9476:Vcan	UTSW	13	89703412	missense	possibly damaging	0.95
R9477:Vcan	UTSW	13	89693009	missense	probably damaging	1.00
R9555:Vcan	UTSW	13	89691540	missense
R9579:Vcan	UTSW	13	89689594	missense	possibly damaging	0.67
R9606:Vcan	UTSW	13	89705372	missense	probably damaging	1.00
R9645:Vcan	UTSW	13	89692962	missense	probably benign	0.00
R9659:Vcan	UTSW	13	89691741	missense	probably damaging	0.99
R9766:Vcan	UTSW	13	89691128	missense	probably benign	0.00
R9778:Vcan	UTSW	13	89689811	missense	probably damaging	1.00
X0058:Vcan	UTSW	13	89692493	missense	probably benign	0.21
X0065:Vcan	UTSW	13	89705749	missense	probably damaging	0.96
Z1176:Vcan	UTSW	13	89692571	missense	probably benign	0.10
Z1177:Vcan	UTSW	13	89703524	missense	probably benign	0.00
Z1177:Vcan	UTSW	13	89703788	nonsense	probably null
Z1177:Vcan	UTSW	13	89704073	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CGGGGATTTGGCTAAAGACC -3'
(R):5'- CCACTTGGAATGCCAGACATATC -3'

Sequencing Primer
(F):5'- TTTGGCTAAAGACCCGAGTGCTC -3'
(R):5'- GGAACTCCGAAGTACAACTGTC -3'

Posted On

2019-06-26