Mutagenetix > Incidental Mutation

Incidental Mutation 'R7302:Vmn1r77'

567041

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r77

Ensembl Gene

ENSMUSG00000095864

Gene Name

vomeronasal 1 receptor 77

Synonyms

Gm6935

MMRRC Submission

045406-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.055) question?

Stock #

R7302 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

11775226-11776146 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 11775983 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Phenylalanine at position 253 (S253F)

Ref Sequence

ENSEMBL: ENSMUSP00000130869 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164446] [ENSMUST00000226525] [ENSMUST00000227320] [ENSMUST00000228213]

AlphaFold

E9PY60

Predicted Effect

possibly damaging
Transcript: ENSMUST00000164446
AA Change: S253F

PolyPhen 2 Score 0.940 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000130869
Gene: ENSMUSG00000095864
AA Change: S253F

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	300	1.9e-12	PFAM
Pfam:V1R	35	299	5.3e-26	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000226525
AA Change: S185F

PolyPhen 2 Score 0.926 (Sensitivity: 0.81; Specificity: 0.94)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000227320
AA Change: S253F

PolyPhen 2 Score 0.940 (Sensitivity: 0.80; Specificity: 0.94)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000228213
AA Change: S185F

PolyPhen 2 Score 0.926 (Sensitivity: 0.81; Specificity: 0.94)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

98% (50/51)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930474N05Rik	C	T	14: 35,817,306 (GRCm39)	P69S	probably benign	Het
Adam17	A	G	12: 21,405,694 (GRCm39)		probably benign	Het
Bcl7a	T	A	5: 123,482,757 (GRCm39)	M22K	probably benign	Het
C6	T	A	15: 4,826,432 (GRCm39)	C672S	probably damaging	Het
Capn11	C	T	17: 45,954,738 (GRCm39)	R133H	probably damaging	Het
Ccdc102a	A	G	8: 95,640,066 (GRCm39)	L76P	probably damaging	Het
Cfhr4	T	C	1: 139,667,436 (GRCm39)		probably null	Het
Cotl1	T	G	8: 120,537,040 (GRCm39)	I125L	probably benign	Het
Dennd5a	A	T	7: 109,504,906 (GRCm39)	M868K	probably damaging	Het
Depdc5	T	C	5: 33,136,852 (GRCm39)	I1374T	probably damaging	Het
Dhx36	T	C	3: 62,386,814 (GRCm39)	Y646C	probably benign	Het
Ecpas	T	C	4: 58,834,593 (GRCm39)	K762E	probably benign	Het
Egln2	A	G	7: 26,864,310 (GRCm39)	V205A	probably damaging	Het
Eri2	T	A	7: 119,386,009 (GRCm39)	M229L	probably benign	Het
Fancf	C	A	7: 51,511,452 (GRCm39)	R184L	probably damaging	Het
Fancl	A	T	11: 26,353,363 (GRCm39)	E86D	probably damaging	Het
Fignl2	T	C	15: 100,951,259 (GRCm39)	D341G	unknown	Het
Gm14410	A	T	2: 176,885,648 (GRCm39)	H205Q	probably damaging	Het
Haus1	T	C	18: 77,848,666 (GRCm39)	N181D	probably benign	Het
Hmbox1	T	C	14: 65,066,115 (GRCm39)	Y285C	probably damaging	Het
Igkv3-9	A	G	6: 70,565,739 (GRCm39)	M113V	probably benign	Het
Ivd	T	C	2: 118,701,985 (GRCm39)	V139A	probably benign	Het
Limch1	A	T	5: 67,116,942 (GRCm39)	Y119F	probably benign	Het
Lrp1	C	T	10: 127,374,856 (GRCm39)	R4534Q	probably benign	Het
Mbd3l2	G	A	9: 18,355,738 (GRCm39)	S21N	probably benign	Het
Mob1b	A	G	5: 88,901,036 (GRCm39)	N148D	probably benign	Het
Mylk4	A	T	13: 32,904,548 (GRCm39)	D195E	probably benign	Het
Ndufa7	T	C	17: 34,048,687 (GRCm39)	S50P	probably benign	Het
Nectin4	G	T	1: 171,214,203 (GRCm39)	E453*	probably null	Het
Nell1	T	G	7: 50,506,017 (GRCm39)	F741L	probably benign	Het
Ntng1	T	A	3: 109,739,933 (GRCm39)	H369L	possibly damaging	Het
Plin4	T	A	17: 56,409,330 (GRCm39)	M1297L	probably benign	Het
Pnp	G	A	14: 51,188,404 (GRCm39)	V193M	probably damaging	Het
Ppp1r10	T	G	17: 36,241,773 (GRCm39)	S849R	unknown	Het
Rpgrip1	A	G	14: 52,387,012 (GRCm39)	E981G	unknown	Het
Sbsn	T	C	7: 30,451,309 (GRCm39)	F108S	probably benign	Het
Scn11a	A	G	9: 119,636,017 (GRCm39)	M310T	probably benign	Het
Sf3b1	A	G	1: 55,055,949 (GRCm39)	S97P	probably benign	Het
Sgsh	A	G	11: 119,238,525 (GRCm39)	V313A	probably benign	Het
Slc25a10	G	T	11: 120,382,782 (GRCm39)		probably benign	Het
Slc9a2	T	A	1: 40,806,828 (GRCm39)	V705E	possibly damaging	Het
Surf2	G	T	2: 26,808,894 (GRCm39)	C116F	probably damaging	Het
Tnks1bp1	T	C	2: 84,882,698 (GRCm39)	I175T	probably benign	Het
Tnxb	C	T	17: 34,897,875 (GRCm39)	T841I	probably benign	Het
Ttll3	A	G	6: 113,386,246 (GRCm39)	D693G	probably damaging	Het
Ust	A	G	10: 8,393,973 (GRCm39)	L64P	probably damaging	Het
Vmn2r66	A	G	7: 84,654,423 (GRCm39)	M512T	probably benign	Het
Zfp13	C	T	17: 23,800,036 (GRCm39)	G89D	probably damaging	Het
Zfp616	T	A	11: 73,976,205 (GRCm39)	Y825N	probably benign	Het
Zfp865	A	G	7: 5,032,252 (GRCm39)	Y79C	possibly damaging	Het
Zfyve26	A	T	12: 79,297,942 (GRCm39)	F1916I	probably damaging	Het

Other mutations in Vmn1r77

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00965:Vmn1r77	APN	7	11,775,223 (GRCm39)	critical splice acceptor site	probably null
IGL00990:Vmn1r77	APN	7	11,775,695 (GRCm39)	missense	probably benign	0.00
IGL00990:Vmn1r77	APN	7	11,775,403 (GRCm39)	missense	probably benign	0.05
IGL01304:Vmn1r77	APN	7	11,775,962 (GRCm39)	missense	probably damaging	1.00
IGL01360:Vmn1r77	APN	7	11,775,315 (GRCm39)	missense	probably benign	0.06
IGL01714:Vmn1r77	APN	7	11,775,277 (GRCm39)	missense	probably benign	0.03
IGL01829:Vmn1r77	APN	7	11,775,358 (GRCm39)	missense	probably damaging	1.00
IGL02336:Vmn1r77	APN	7	11,775,223 (GRCm39)	critical splice acceptor site	probably null
R0456:Vmn1r77	UTSW	7	11,775,665 (GRCm39)	nonsense	probably null
R0622:Vmn1r77	UTSW	7	11,775,315 (GRCm39)	missense	probably benign	0.06
R1244:Vmn1r77	UTSW	7	11,775,847 (GRCm39)	missense	possibly damaging	0.59
R1696:Vmn1r77	UTSW	7	11,775,547 (GRCm39)	nonsense	probably null
R1836:Vmn1r77	UTSW	7	11,775,338 (GRCm39)	missense	probably benign	0.00
R1898:Vmn1r77	UTSW	7	11,775,550 (GRCm39)	missense	probably damaging	1.00
R4533:Vmn1r77	UTSW	7	11,775,756 (GRCm39)	missense	probably benign	0.02
R4668:Vmn1r77	UTSW	7	11,775,358 (GRCm39)	missense	probably damaging	1.00
R5381:Vmn1r77	UTSW	7	11,775,952 (GRCm39)	missense	probably damaging	1.00
R6290:Vmn1r77	UTSW	7	11,775,736 (GRCm39)	missense	probably damaging	1.00
R6675:Vmn1r77	UTSW	7	11,775,382 (GRCm39)	missense	probably damaging	1.00
R7032:Vmn1r77	UTSW	7	11,776,017 (GRCm39)	nonsense	probably null
R7044:Vmn1r77	UTSW	7	11,775,761 (GRCm39)	missense	probably benign	0.06
R7417:Vmn1r77	UTSW	7	11,775,611 (GRCm39)	missense	probably damaging	1.00
R7436:Vmn1r77	UTSW	7	11,775,694 (GRCm39)	missense	probably benign	0.01
R8487:Vmn1r77	UTSW	7	11,775,514 (GRCm39)	missense	probably damaging	1.00
R8862:Vmn1r77	UTSW	7	11,776,060 (GRCm39)	missense	probably benign	0.19
R9614:Vmn1r77	UTSW	7	11,775,766 (GRCm39)	missense	probably benign	0.43
R9753:Vmn1r77	UTSW	7	11,775,659 (GRCm39)	missense	probably damaging	1.00
Z1176:Vmn1r77	UTSW	7	11,775,695 (GRCm39)	missense	probably benign	0.00
Z1176:Vmn1r77	UTSW	7	11,775,674 (GRCm39)	missense
Z1176:Vmn1r77	UTSW	7	11,775,524 (GRCm39)	missense	probably benign	0.36
Z1176:Vmn1r77	UTSW	7	11,775,508 (GRCm39)	missense
Z1177:Vmn1r77	UTSW	7	11,775,695 (GRCm39)	missense	probably benign	0.00
Z1177:Vmn1r77	UTSW	7	11,775,674 (GRCm39)	missense
Z1177:Vmn1r77	UTSW	7	11,775,524 (GRCm39)	missense	probably benign	0.36

Predicted Primers

PCR Primer
(F):5'- TTCTGATCATGTGCGAGCTG -3'
(R):5'- GTGGTAGTTATCTCTTACCAAAGC -3'

Sequencing Primer
(F):5'- ATCATGTGCGAGCTGTACTC -3'
(R):5'- TCTTACCAAAGCACGGTAAAAACAGG -3'

Posted On

2019-06-26