Mutagenetix > Incidental Mutation

Incidental Mutation 'R0636:Vcan'

56706

Institutional Source

Beutler Lab

Gene Symbol

Vcan

Ensembl Gene

ENSMUSG00000021614

Gene Name

versican

Synonyms

PG-M, hdf, DPEAAE, heart defect, Cspg2, 5430420N07Rik

MMRRC Submission

038825-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0636 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

89655312-89742509 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 89712267 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Leucine at position 327 (R327L)

Ref Sequence

ENSEMBL: ENSMUSP00000105173 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109543] [ENSMUST00000109544] [ENSMUST00000109546] [ENSMUST00000159337] [ENSMUST00000159910]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000109543
AA Change: R327L

PolyPhen 2 Score 0.105 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000105170
Gene: ENSMUSG00000021614
AA Change: R327L

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
EGF	351	384	2.72e-7	SMART
EGF_CA	386	422	1.16e-10	SMART
CLECT	428	549	3.08e-34	SMART
CCP	555	611	1.04e-8	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000109544
AA Change: R327L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000105171
Gene: ENSMUSG00000021614
AA Change: R327L

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
EGF	1311	1344	2.72e-7	SMART
EGF_CA	1346	1382	1.16e-10	SMART
CLECT	1388	1509	3.08e-34	SMART
CCP	1515	1571	1.04e-8	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000109546
AA Change: R327L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000105173
Gene: ENSMUSG00000021614
AA Change: R327L

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
low complexity region	1322	1333	N/A	INTRINSIC
low complexity region	1546	1569	N/A	INTRINSIC
low complexity region	1837	1852	N/A	INTRINSIC
low complexity region	2013	2026	N/A	INTRINSIC
low complexity region	2354	2367	N/A	INTRINSIC
low complexity region	2468	2482	N/A	INTRINSIC
low complexity region	2719	2728	N/A	INTRINSIC
EGF	3050	3083	2.72e-7	SMART
EGF_CA	3085	3121	1.16e-10	SMART
CLECT	3127	3248	3.08e-34	SMART
CCP	3254	3310	1.04e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000159337
AA Change: R327L

PolyPhen 2 Score 0.053 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000125432
Gene: ENSMUSG00000021614
AA Change: R327L

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	354	365	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000159910
AA Change: R327L

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000125446
Gene: ENSMUSG00000021614
AA Change: R327L

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	362	373	N/A	INTRINSIC
low complexity region	586	609	N/A	INTRINSIC
low complexity region	877	892	N/A	INTRINSIC
low complexity region	1053	1066	N/A	INTRINSIC
low complexity region	1394	1407	N/A	INTRINSIC
low complexity region	1508	1522	N/A	INTRINSIC
low complexity region	1759	1768	N/A	INTRINSIC
EGF	2090	2123	2.72e-7	SMART
EGF_CA	2125	2161	1.16e-10	SMART
CLECT	2167	2288	3.08e-34	SMART
CCP	2294	2350	1.04e-8	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000160029

Meta Mutation Damage Score

0.2388

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.7%
20x: 95.7%

Validation Efficiency

96% (74/77)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
PHENOTYPE: Homozygotes for an insertional mutation exhibit anterior-posterior segmental defects of the heart, lack endocardial cushions of the conus and atrioventricular region, and die and around embryonic day 10.5. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921539E11Rik	T	C	4: 103,231,217 (GRCm38)	Y264C	probably damaging	Het
8030423J24Rik	T	C	13: 70,884,225 (GRCm38)	F139L	unknown	Het
Aco1	A	G	4: 40,175,697 (GRCm38)	E146G	probably damaging	Het
Adam2	T	G	14: 66,034,816 (GRCm38)	D639A	probably benign	Het
Adh4	G	T	3: 138,428,074 (GRCm38)	R315L	probably damaging	Het
Adprhl1	T	C	8: 13,248,702 (GRCm38)	D76G	probably damaging	Het
AF366264	T	C	8: 13,837,870 (GRCm38)	R74G	probably benign	Het
Akip1	T	C	7: 109,707,519 (GRCm38)		probably benign	Het
Ap3d1	T	A	10: 80,719,382 (GRCm38)	K370*	probably null	Het
Arfgef1	C	A	1: 10,199,851 (GRCm38)	V358L	probably benign	Het
Arpp21	A	T	9: 112,183,498 (GRCm38)	D85E	probably benign	Het
Azi2	A	T	9: 118,062,057 (GRCm38)	L383F	probably benign	Het
Bpgm	T	A	6: 34,504,287 (GRCm38)	D206E	probably benign	Het
Bsn	T	C	9: 108,107,834 (GRCm38)	D3007G	unknown	Het
Ccdc142	T	G	6: 83,107,198 (GRCm38)		probably benign	Het
Cep135	T	C	5: 76,615,657 (GRCm38)	V498A	probably benign	Het
Cntn6	A	T	6: 104,863,148 (GRCm38)	Q1003L	probably benign	Het
Cntnap2	T	A	6: 47,296,708 (GRCm38)		probably benign	Het
Csf2rb2	G	A	15: 78,291,960 (GRCm38)	Q139*	probably null	Het
Cyp3a16	A	G	5: 145,463,085 (GRCm38)	V101A	probably benign	Het
D630045J12Rik	T	C	6: 38,196,778 (GRCm38)	T152A	probably benign	Het
Def8	G	A	8: 123,454,357 (GRCm38)	W176*	probably null	Het
Dgkg	A	G	16: 22,579,729 (GRCm38)		probably benign	Het
Ear10	T	C	14: 43,922,994 (GRCm38)		probably null	Het
Fbxw2	A	T	2: 34,822,847 (GRCm38)	Y67*	probably null	Het
Flii	T	A	11: 60,715,552 (GRCm38)	Y1104F	probably damaging	Het
Gm973	G	A	1: 59,551,144 (GRCm38)	R270K	probably benign	Het
Gm9833	T	C	3: 10,088,783 (GRCm38)	L204P	possibly damaging	Het
Gnl3	T	A	14: 31,017,153 (GRCm38)	K75N	probably damaging	Het
Gpc6	A	T	14: 117,624,493 (GRCm38)	M274L	probably benign	Het
Ifi47	A	G	11: 49,096,651 (GRCm38)	E415G	possibly damaging	Het
Ift57	A	G	16: 49,711,896 (GRCm38)	T130A	probably benign	Het
Itpr2	T	A	6: 146,171,412 (GRCm38)	D2373V	probably damaging	Het
Kat6a	T	C	8: 22,939,323 (GRCm38)	S1565P	possibly damaging	Het
Klhl6	A	T	16: 19,948,073 (GRCm38)		probably benign	Het
Klra2	T	C	6: 131,220,104 (GRCm38)		probably benign	Het
Lama5	A	G	2: 180,189,331 (GRCm38)		probably null	Het
Mapk4	A	G	18: 73,930,454 (GRCm38)	S566P	probably benign	Het
Mindy4	C	A	6: 55,276,585 (GRCm38)	R480S	possibly damaging	Het
Mterf3	T	C	13: 66,922,753 (GRCm38)		probably benign	Het
Mtmr2	A	G	9: 13,801,913 (GRCm38)		probably null	Het
Naip5	T	C	13: 100,219,688 (GRCm38)	T1140A	probably benign	Het
Nf1	A	G	11: 79,535,703 (GRCm38)	T1648A	probably damaging	Het
Nlk	A	T	11: 78,695,844 (GRCm38)	D141E	probably benign	Het
Noxa1	C	A	2: 25,086,094 (GRCm38)		probably benign	Het
Olfr1279	A	G	2: 111,306,412 (GRCm38)	N69S	probably benign	Het
Olfr1480	A	T	19: 13,530,249 (GRCm38)	Y236F	possibly damaging	Het
Olfr476	T	C	7: 107,967,472 (GRCm38)	V25A	probably benign	Het
Otog	G	A	7: 46,264,228 (GRCm38)		probably null	Het
Pebp4	T	C	14: 70,048,347 (GRCm38)		probably benign	Het
Phgdh	G	T	3: 98,333,291 (GRCm38)	N100K	possibly damaging	Het
Pnisr	T	A	4: 21,873,800 (GRCm38)		probably benign	Het
Ptpn6	T	C	6: 124,725,279 (GRCm38)	H346R	probably benign	Het
Rsf1	T	C	7: 97,662,019 (GRCm38)	V652A	possibly damaging	Het
Rubcn	G	A	16: 32,828,686 (GRCm38)	H624Y	probably damaging	Het
Setdb2	T	C	14: 59,406,704 (GRCm38)	N656D	probably benign	Het
Slc22a23	T	C	13: 34,299,093 (GRCm38)	T268A	probably benign	Het
Slc3a1	A	T	17: 85,032,794 (GRCm38)	T215S	possibly damaging	Het
Srsf2	A	G	11: 116,852,078 (GRCm38)	S206P	probably benign	Het
Susd2	T	A	10: 75,639,350 (GRCm38)	D542V	probably damaging	Het
Svep1	G	A	4: 58,073,121 (GRCm38)	Q2063*	probably null	Het
Syne2	G	A	12: 75,930,983 (GRCm38)	V1401M	possibly damaging	Het
Tenm2	A	G	11: 36,943,976 (GRCm38)	L64P	probably damaging	Het
Tigd2	A	G	6: 59,211,287 (GRCm38)	T380A	possibly damaging	Het
Trmt12	G	T	15: 58,873,985 (GRCm38)	V411F	probably damaging	Het
Ubr4	T	C	4: 139,436,302 (GRCm38)		probably null	Het
Ush2a	G	A	1: 188,822,738 (GRCm38)	C3571Y	probably benign	Het
Usp8	A	G	2: 126,720,110 (GRCm38)	M75V	possibly damaging	Het
Vps8	A	G	16: 21,434,933 (GRCm38)	E8G	probably benign	Het
Washc5	T	C	15: 59,359,409 (GRCm38)	D335G	probably benign	Het
Zbtb39	A	G	10: 127,742,835 (GRCm38)	N426S	probably benign	Het
Zfp184	T	A	13: 21,949,749 (GRCm38)	D55E	probably damaging	Het
Zfp882	T	C	8: 71,914,337 (GRCm38)	V336A	probably benign	Het

Other mutations in Vcan

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00434:Vcan	APN	13	89,704,702 (GRCm38)	missense	probably damaging	1.00
IGL00502:Vcan	APN	13	89,692,319 (GRCm38)	missense	probably benign
IGL00504:Vcan	APN	13	89,691,275 (GRCm38)	missense	possibly damaging	0.70
IGL00566:Vcan	APN	13	89,688,979 (GRCm38)	missense	probably benign	0.01
IGL00701:Vcan	APN	13	89,703,726 (GRCm38)	missense	probably benign
IGL00743:Vcan	APN	13	89,725,306 (GRCm38)	missense	probably damaging	0.98
IGL00962:Vcan	APN	13	89,662,052 (GRCm38)	missense	probably damaging	1.00
IGL01085:Vcan	APN	13	89,679,958 (GRCm38)	missense	probably damaging	1.00
IGL01317:Vcan	APN	13	89,691,668 (GRCm38)	missense	probably benign	0.00
IGL01349:Vcan	APN	13	89,703,943 (GRCm38)	missense	probably damaging	0.98
IGL01391:Vcan	APN	13	89,704,169 (GRCm38)	missense	probably benign	0.19
IGL01644:Vcan	APN	13	89,688,675 (GRCm38)	missense	probably benign	0.13
IGL01657:Vcan	APN	13	89,690,586 (GRCm38)	missense	probably damaging	1.00
IGL01707:Vcan	APN	13	89,689,745 (GRCm38)	missense	probably damaging	1.00
IGL01764:Vcan	APN	13	89,725,388 (GRCm38)	missense	probably damaging	1.00
IGL01920:Vcan	APN	13	89,689,205 (GRCm38)	missense	probably benign	0.04
IGL01989:Vcan	APN	13	89,689,359 (GRCm38)	missense	possibly damaging	0.86
IGL01999:Vcan	APN	13	89,684,438 (GRCm38)	missense	probably damaging	1.00
IGL02083:Vcan	APN	13	89,725,565 (GRCm38)	missense	probably damaging	1.00
IGL02160:Vcan	APN	13	89,684,493 (GRCm38)	missense	probably damaging	1.00
IGL02217:Vcan	APN	13	89,703,077 (GRCm38)	missense	probably damaging	1.00
IGL02522:Vcan	APN	13	89,704,849 (GRCm38)	missense	probably benign	0.00
IGL02527:Vcan	APN	13	89,690,657 (GRCm38)	missense	possibly damaging	0.95
IGL02926:Vcan	APN	13	89,688,623 (GRCm38)	missense	probably damaging	0.98
IGL03061:Vcan	APN	13	89,703,275 (GRCm38)	missense	probably benign	0.25
IGL03331:Vcan	APN	13	89,661,932 (GRCm38)	missense	probably damaging	1.00
IGL03352:Vcan	APN	13	89,705,006 (GRCm38)	missense	probably benign	0.00
R0041:Vcan	UTSW	13	89,661,985 (GRCm38)	missense	probably damaging	1.00
R0102:Vcan	UTSW	13	89,703,668 (GRCm38)	missense	probably benign	0.01
R0102:Vcan	UTSW	13	89,703,668 (GRCm38)	missense	probably benign	0.01
R0109:Vcan	UTSW	13	89,678,073 (GRCm38)	critical splice donor site	probably null
R0139:Vcan	UTSW	13	89,691,261 (GRCm38)	missense	probably damaging	1.00
R0295:Vcan	UTSW	13	89,712,191 (GRCm38)	missense	probably benign	0.06
R0375:Vcan	UTSW	13	89,691,275 (GRCm38)	missense	probably damaging	0.99
R0379:Vcan	UTSW	13	89,703,546 (GRCm38)	missense	probably damaging	0.99
R0457:Vcan	UTSW	13	89,703,199 (GRCm38)	missense	possibly damaging	0.78
R0482:Vcan	UTSW	13	89,678,145 (GRCm38)	missense	probably damaging	1.00
R0485:Vcan	UTSW	13	89,704,660 (GRCm38)	missense	possibly damaging	0.92
R0532:Vcan	UTSW	13	89,703,772 (GRCm38)	missense	probably damaging	0.99
R0561:Vcan	UTSW	13	89,731,464 (GRCm38)	missense	possibly damaging	0.86
R0561:Vcan	UTSW	13	89,712,253 (GRCm38)	missense	probably damaging	1.00
R0636:Vcan	UTSW	13	89,704,706 (GRCm38)	missense	probably damaging	0.99
R0680:Vcan	UTSW	13	89,679,822 (GRCm38)	missense	probably damaging	1.00
R0849:Vcan	UTSW	13	89,704,953 (GRCm38)	missense	possibly damaging	0.75
R1006:Vcan	UTSW	13	89,685,077 (GRCm38)	critical splice donor site	probably null
R1104:Vcan	UTSW	13	89,692,410 (GRCm38)	missense	probably damaging	1.00
R1118:Vcan	UTSW	13	89,705,663 (GRCm38)	missense	probably damaging	1.00
R1137:Vcan	UTSW	13	89,704,303 (GRCm38)	missense	probably damaging	1.00
R1199:Vcan	UTSW	13	89,679,794 (GRCm38)	splice site	probably null
R1219:Vcan	UTSW	13	89,679,904 (GRCm38)	missense	probably damaging	1.00
R1296:Vcan	UTSW	13	89,657,556 (GRCm38)	missense	probably damaging	1.00
R1332:Vcan	UTSW	13	89,693,055 (GRCm38)	missense	probably damaging	1.00
R1336:Vcan	UTSW	13	89,693,055 (GRCm38)	missense	probably damaging	1.00
R1403:Vcan	UTSW	13	89,688,484 (GRCm38)	missense	probably benign	0.00
R1403:Vcan	UTSW	13	89,688,484 (GRCm38)	missense	probably benign	0.00
R1546:Vcan	UTSW	13	89,692,956 (GRCm38)	missense	probably damaging	0.99
R1604:Vcan	UTSW	13	89,689,661 (GRCm38)	missense	probably benign	0.42
R1616:Vcan	UTSW	13	89,705,663 (GRCm38)	missense	probably damaging	1.00
R1636:Vcan	UTSW	13	89,703,667 (GRCm38)	missense	possibly damaging	0.90
R1654:Vcan	UTSW	13	89,661,946 (GRCm38)	missense	probably damaging	1.00
R1680:Vcan	UTSW	13	89,703,547 (GRCm38)	missense	probably benign	0.19
R1694:Vcan	UTSW	13	89,688,483 (GRCm38)	missense	probably damaging	0.98
R1712:Vcan	UTSW	13	89,721,775 (GRCm38)	missense	probably damaging	1.00
R1754:Vcan	UTSW	13	89,704,735 (GRCm38)	missense	probably benign	0.01
R1756:Vcan	UTSW	13	89,691,681 (GRCm38)	missense	probably benign	0.05
R1824:Vcan	UTSW	13	89,705,212 (GRCm38)	missense	possibly damaging	0.75
R1852:Vcan	UTSW	13	89,705,392 (GRCm38)	missense	probably damaging	0.99
R1868:Vcan	UTSW	13	89,690,871 (GRCm38)	missense	probably benign	0.12
R1920:Vcan	UTSW	13	89,693,015 (GRCm38)	missense	probably damaging	1.00
R1932:Vcan	UTSW	13	89,705,534 (GRCm38)	missense	possibly damaging	0.78
R1934:Vcan	UTSW	13	89,702,926 (GRCm38)	missense	probably damaging	1.00
R1942:Vcan	UTSW	13	89,703,424 (GRCm38)	missense	probably benign	0.01
R1964:Vcan	UTSW	13	89,692,742 (GRCm38)	missense	probably benign	0.02
R1970:Vcan	UTSW	13	89,689,038 (GRCm38)	missense	probably damaging	1.00
R2045:Vcan	UTSW	13	89,690,985 (GRCm38)	missense	probably benign	0.00
R2110:Vcan	UTSW	13	89,693,303 (GRCm38)	missense	probably damaging	1.00
R2111:Vcan	UTSW	13	89,693,303 (GRCm38)	missense	probably damaging	1.00
R2112:Vcan	UTSW	13	89,693,303 (GRCm38)	missense	probably damaging	1.00
R2136:Vcan	UTSW	13	89,689,737 (GRCm38)	missense	probably damaging	1.00
R2158:Vcan	UTSW	13	89,703,529 (GRCm38)	missense	possibly damaging	0.68
R2376:Vcan	UTSW	13	89,703,410 (GRCm38)	missense	possibly damaging	0.80
R2385:Vcan	UTSW	13	89,689,449 (GRCm38)	missense	probably damaging	1.00
R2443:Vcan	UTSW	13	89,704,675 (GRCm38)	missense	probably damaging	1.00
R2876:Vcan	UTSW	13	89,704,237 (GRCm38)	missense	probably damaging	1.00
R3607:Vcan	UTSW	13	89,703,301 (GRCm38)	missense	probably damaging	0.98
R4042:Vcan	UTSW	13	89,692,543 (GRCm38)	missense	probably benign	0.35
R4043:Vcan	UTSW	13	89,692,543 (GRCm38)	missense	probably benign	0.35
R4044:Vcan	UTSW	13	89,692,543 (GRCm38)	missense	probably benign	0.35
R4065:Vcan	UTSW	13	89,679,887 (GRCm38)	missense	probably damaging	1.00
R4161:Vcan	UTSW	13	89,685,158 (GRCm38)	missense	probably damaging	1.00
R4178:Vcan	UTSW	13	89,725,547 (GRCm38)	missense	probably damaging	1.00
R4290:Vcan	UTSW	13	89,725,486 (GRCm38)	missense	probably damaging	1.00
R4530:Vcan	UTSW	13	89,704,028 (GRCm38)	missense	probably damaging	0.97
R4666:Vcan	UTSW	13	89,679,934 (GRCm38)	missense	probably damaging	1.00
R4785:Vcan	UTSW	13	89,705,789 (GRCm38)	missense	probably damaging	1.00
R4870:Vcan	UTSW	13	89,704,739 (GRCm38)	missense	probably benign	0.01
R4973:Vcan	UTSW	13	89,688,842 (GRCm38)	missense	probably benign	0.30
R5037:Vcan	UTSW	13	89,703,977 (GRCm38)	missense	probably damaging	1.00
R5104:Vcan	UTSW	13	89,657,472 (GRCm38)	intron	probably benign
R5124:Vcan	UTSW	13	89,725,517 (GRCm38)	missense	probably damaging	1.00
R5129:Vcan	UTSW	13	89,690,240 (GRCm38)	missense	probably damaging	1.00
R5198:Vcan	UTSW	13	89,690,872 (GRCm38)	missense	probably damaging	1.00
R5240:Vcan	UTSW	13	89,692,532 (GRCm38)	missense	probably benign	0.08
R5254:Vcan	UTSW	13	89,691,600 (GRCm38)	missense	probably damaging	0.99
R5280:Vcan	UTSW	13	89,690,286 (GRCm38)	missense	probably benign	0.00
R5522:Vcan	UTSW	13	89,691,810 (GRCm38)	missense	possibly damaging	0.62
R5557:Vcan	UTSW	13	89,703,112 (GRCm38)	missense	possibly damaging	0.77
R5568:Vcan	UTSW	13	89,688,671 (GRCm38)	missense	probably damaging	1.00
R5578:Vcan	UTSW	13	89,691,503 (GRCm38)	missense	probably benign	0.01
R5627:Vcan	UTSW	13	89,691,135 (GRCm38)	frame shift	probably null
R5687:Vcan	UTSW	13	89,678,134 (GRCm38)	missense	probably damaging	1.00
R5752:Vcan	UTSW	13	89,679,950 (GRCm38)	missense	probably damaging	1.00
R5879:Vcan	UTSW	13	89,703,952 (GRCm38)	missense	probably damaging	0.99
R5941:Vcan	UTSW	13	89,692,691 (GRCm38)	missense	probably damaging	0.98
R6113:Vcan	UTSW	13	89,657,536 (GRCm38)	nonsense	probably null
R6135:Vcan	UTSW	13	89,689,926 (GRCm38)	missense	probably benign	0.36
R6252:Vcan	UTSW	13	89,691,220 (GRCm38)	nonsense	probably null
R6280:Vcan	UTSW	13	89,725,373 (GRCm38)	missense	probably damaging	1.00
R6317:Vcan	UTSW	13	89,691,597 (GRCm38)	missense	probably benign	0.22
R6327:Vcan	UTSW	13	89,704,832 (GRCm38)	missense	probably damaging	0.99
R6460:Vcan	UTSW	13	89,690,687 (GRCm38)	missense	possibly damaging	0.61
R6669:Vcan	UTSW	13	89,704,731 (GRCm38)	missense	probably benign	0.21
R6744:Vcan	UTSW	13	89,705,182 (GRCm38)	missense	probably damaging	1.00
R6819:Vcan	UTSW	13	89,705,125 (GRCm38)	missense	probably benign	0.00
R6880:Vcan	UTSW	13	89,712,381 (GRCm38)	missense	probably damaging	1.00
R6956:Vcan	UTSW	13	89,689,431 (GRCm38)	missense	probably damaging	0.99
R6971:Vcan	UTSW	13	89,678,133 (GRCm38)	missense	probably damaging	1.00
R6985:Vcan	UTSW	13	89,679,956 (GRCm38)	missense	probably damaging	1.00
R6994:Vcan	UTSW	13	89,693,407 (GRCm38)	missense	possibly damaging	0.94
R6997:Vcan	UTSW	13	89,690,618 (GRCm38)	missense	probably damaging	0.98
R7029:Vcan	UTSW	13	89,690,241 (GRCm38)	missense	probably damaging	1.00
R7066:Vcan	UTSW	13	89,705,686 (GRCm38)	missense	probably damaging	1.00
R7156:Vcan	UTSW	13	89,689,110 (GRCm38)	missense	possibly damaging	0.95
R7171:Vcan	UTSW	13	89,725,591 (GRCm38)	missense	probably damaging	1.00
R7176:Vcan	UTSW	13	89,688,936 (GRCm38)	missense	probably benign	0.01
R7229:Vcan	UTSW	13	89,705,270 (GRCm38)	missense	possibly damaging	0.87
R7250:Vcan	UTSW	13	89,731,457 (GRCm38)	critical splice donor site	probably null
R7250:Vcan	UTSW	13	89,721,686 (GRCm38)	missense	probably damaging	1.00
R7262:Vcan	UTSW	13	89,705,161 (GRCm38)	missense	possibly damaging	0.62
R7289:Vcan	UTSW	13	89,692,733 (GRCm38)	nonsense	probably null
R7299:Vcan	UTSW	13	89,705,266 (GRCm38)	missense	probably benign
R7301:Vcan	UTSW	13	89,705,266 (GRCm38)	missense	probably benign
R7425:Vcan	UTSW	13	89,689,832 (GRCm38)	missense	probably damaging	0.99
R7514:Vcan	UTSW	13	89,704,118 (GRCm38)	missense	probably damaging	0.97
R7579:Vcan	UTSW	13	89,692,458 (GRCm38)	missense	probably damaging	1.00
R7618:Vcan	UTSW	13	89,692,223 (GRCm38)	missense	probably damaging	0.99
R7655:Vcan	UTSW	13	89,685,114 (GRCm38)	missense	probably damaging	1.00
R7656:Vcan	UTSW	13	89,685,114 (GRCm38)	missense	probably damaging	1.00
R7676:Vcan	UTSW	13	89,691,789 (GRCm38)	missense	probably damaging	1.00
R7719:Vcan	UTSW	13	89,704,619 (GRCm38)	missense	probably damaging	0.98
R7753:Vcan	UTSW	13	89,689,323 (GRCm38)	missense	probably damaging	1.00
R7762:Vcan	UTSW	13	89,692,937 (GRCm38)	missense	probably damaging	1.00
R7778:Vcan	UTSW	13	89,688,654 (GRCm38)	missense	probably damaging	1.00
R7824:Vcan	UTSW	13	89,688,654 (GRCm38)	missense	probably damaging	1.00
R7995:Vcan	UTSW	13	89,691,858 (GRCm38)	missense	probably benign
R7998:Vcan	UTSW	13	89,704,327 (GRCm38)	missense	probably damaging	1.00
R8033:Vcan	UTSW	13	89,704,360 (GRCm38)	missense	probably benign	0.04
R8061:Vcan	UTSW	13	89,657,290 (GRCm38)	missense	probably benign	0.45
R8103:Vcan	UTSW	13	89,703,320 (GRCm38)	nonsense	probably null
R8103:Vcan	UTSW	13	89,657,658 (GRCm38)	missense	probably damaging	1.00
R8124:Vcan	UTSW	13	89,704,254 (GRCm38)	missense	possibly damaging	0.93
R8162:Vcan	UTSW	13	89,704,987 (GRCm38)	nonsense	probably null
R8166:Vcan	UTSW	13	89,692,736 (GRCm38)	missense	probably benign	0.02
R8274:Vcan	UTSW	13	89,704,970 (GRCm38)	missense	probably benign	0.02
R8284:Vcan	UTSW	13	89,704,335 (GRCm38)	missense	possibly damaging	0.68
R8417:Vcan	UTSW	13	89,688,743 (GRCm38)	missense	probably benign	0.19
R8696:Vcan	UTSW	13	89,691,098 (GRCm38)	missense	probably benign	0.00
R8738:Vcan	UTSW	13	89,692,320 (GRCm38)	missense	probably benign	0.17
R8792:Vcan	UTSW	13	89,692,111 (GRCm38)	missense	possibly damaging	0.91
R8887:Vcan	UTSW	13	89,704,907 (GRCm38)	missense	probably benign
R9049:Vcan	UTSW	13	89,678,105 (GRCm38)	missense	probably damaging	1.00
R9074:Vcan	UTSW	13	89,691,027 (GRCm38)	missense	possibly damaging	0.95
R9095:Vcan	UTSW	13	89,704,525 (GRCm38)	missense	probably benign	0.32
R9172:Vcan	UTSW	13	89,679,931 (GRCm38)	missense	probably damaging	1.00
R9199:Vcan	UTSW	13	89,690,496 (GRCm38)	nonsense	probably null
R9259:Vcan	UTSW	13	89,690,870 (GRCm38)	missense	probably damaging	0.99
R9455:Vcan	UTSW	13	89,689,333 (GRCm38)	missense	probably damaging	1.00
R9476:Vcan	UTSW	13	89,703,412 (GRCm38)	missense	possibly damaging	0.95
R9477:Vcan	UTSW	13	89,693,009 (GRCm38)	missense	probably damaging	1.00
R9555:Vcan	UTSW	13	89,691,540 (GRCm38)	missense
R9579:Vcan	UTSW	13	89,689,594 (GRCm38)	missense	possibly damaging	0.67
R9606:Vcan	UTSW	13	89,705,372 (GRCm38)	missense	probably damaging	1.00
R9645:Vcan	UTSW	13	89,692,962 (GRCm38)	missense	probably benign	0.00
R9659:Vcan	UTSW	13	89,691,741 (GRCm38)	missense	probably damaging	0.99
R9766:Vcan	UTSW	13	89,691,128 (GRCm38)	missense	probably benign	0.00
R9778:Vcan	UTSW	13	89,689,811 (GRCm38)	missense	probably damaging	1.00
X0058:Vcan	UTSW	13	89,692,493 (GRCm38)	missense	probably benign	0.21
X0065:Vcan	UTSW	13	89,705,749 (GRCm38)	missense	probably damaging	0.96
Z1176:Vcan	UTSW	13	89,692,571 (GRCm38)	missense	probably benign	0.10
Z1177:Vcan	UTSW	13	89,704,073 (GRCm38)	missense	probably damaging	1.00
Z1177:Vcan	UTSW	13	89,703,788 (GRCm38)	nonsense	probably null
Z1177:Vcan	UTSW	13	89,703,524 (GRCm38)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TGTGTCACTGCACTGTAGGTCCTC -3'
(R):5'- CCACATCACTGCTCCCAGTAAGTTC -3'

Sequencing Primer
(F):5'- GGTCCTCCATGCACTTAGTACAG -3'
(R):5'- TTCGAGGAGGCCGAAGC -3'

Posted On

2013-07-11