Mutagenetix > Incidental Mutations

Incidental Mutation 'R7304:Wdr90'

567198

Institutional Source

Beutler Lab

Gene Symbol

Wdr90

Ensembl Gene

ENSMUSG00000073434

Gene Name

WD repeat domain 90

Synonyms

3230401M21Rik

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.117) question?

Stock #

R7304 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

25844771-25861501 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 25851506 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 1105 (D1105G)

Ref Sequence

ENSEMBL: ENSMUSP00000078426 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079461] [ENSMUST00000176923]

AlphaFold

Q6ZPG2

Predicted Effect

probably benign
Transcript: ENSMUST00000079461
AA Change: D1105G

PolyPhen 2 Score 0.103 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000078426
Gene: ENSMUSG00000073434
AA Change: D1105G

Domain	Start	End	E-Value	Type
low complexity region	32	53	N/A	INTRINSIC
Pfam:DUF667	60	183	5e-26	PFAM
Pfam:DUF667	210	271	3.7e-9	PFAM
low complexity region	381	396	N/A	INTRINSIC
WD40	478	519	5.94e0	SMART
WD40	522	565	3.2e0	SMART
WD40	572	612	3.3e1	SMART
WD40	687	725	1.15e1	SMART
WD40	728	766	5.75e-1	SMART
WD40	768	808	9.24e-4	SMART
WD40	811	850	4.13e0	SMART
WD40	853	892	4.62e-1	SMART
WD40	950	993	1.07e1	SMART
WD40	996	1035	5.75e-1	SMART
WD40	1040	1077	1.58e-2	SMART
WD40	1290	1334	5.23e-3	SMART
WD40	1337	1378	1.27e-1	SMART
WD40	1384	1419	1.83e2	SMART
WD40	1422	1469	3.08e0	SMART
WD40	1472	1509	9.9e0	SMART
WD40	1568	1607	9.02e-7	SMART
WD40	1610	1655	5.75e-1	SMART
WD40	1659	1697	2.98e-1	SMART
WD40	1700	1749	6.14e1	SMART
WD40	1850	1888	1.92e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000176678

Predicted Effect

probably benign
Transcript: ENSMUST00000176923
AA Change: D1087G

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000135420
Gene: ENSMUSG00000073434
AA Change: D1087G

Domain	Start	End	E-Value	Type
low complexity region	32	53	N/A	INTRINSIC
Pfam:DUF667	60	252	5.1e-14	PFAM
low complexity region	363	378	N/A	INTRINSIC
WD40	460	501	5.94e0	SMART
WD40	504	547	3.2e0	SMART
WD40	554	594	3.3e1	SMART
WD40	669	707	1.15e1	SMART
WD40	710	748	5.75e-1	SMART
WD40	750	790	9.24e-4	SMART
WD40	793	832	4.13e0	SMART
WD40	835	874	4.62e-1	SMART
WD40	932	975	1.07e1	SMART
WD40	978	1017	5.75e-1	SMART
WD40	1022	1059	1.58e-2	SMART
WD40	1272	1316	5.23e-3	SMART
WD40	1319	1360	1.27e-1	SMART
WD40	1366	1401	1.83e2	SMART
WD40	1404	1451	3.08e0	SMART
WD40	1454	1491	9.9e0	SMART
WD40	1550	1589	9.02e-7	SMART
WD40	1592	1637	5.75e-1	SMART
WD40	1641	1679	2.98e-1	SMART
WD40	1682	1731	6.14e1	SMART
WD40	1832	1870	1.92e-1	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

100% (66/66)

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110008L16Rik	A	G	12: 55,304,644	D246G	probably damaging	Het
Abca13	A	G	11: 9,297,203	S2317G	probably benign	Het
Acap2	A	C	16: 31,108,116	L502R	probably benign	Het
Amotl2	A	G	9: 102,728,350	E467G	probably damaging	Het
Apbb1ip	A	T	2: 22,853,135		probably null	Het
Armc9	C	G	1: 86,162,715	D77E	probably benign	Het
Art1	T	A	7: 102,106,324	S8T	possibly damaging	Het
Asic5	G	A	3: 82,009,565	A321T	possibly damaging	Het
Astn2	A	G	4: 66,185,375	I267T	unknown	Het
Bmp8a	T	C	4: 123,342,389	N107S	probably benign	Het
Card14	T	A	11: 119,337,747	L633Q	probably damaging	Het
Chpf	C	T	1: 75,479,054	V18M	possibly damaging	Het
Cog7	T	C	7: 121,937,139	I493V	probably benign	Het
Col3a1	A	T	1: 45,347,811	N1394I	unknown	Het
Crybg1	C	T	10: 43,997,258	D1285N	probably benign	Het
Depdc7	A	G	2: 104,723,118	V395A	possibly damaging	Het
Dido1	G	A	2: 180,687,493	L379F	probably damaging	Het
Dnajc13	G	T	9: 104,238,514	T32N	probably benign	Het
Dok2	T	A	14: 70,776,028	S133R	probably benign	Het
Ehbp1l1	C	T	19: 5,716,382	R1311H	probably damaging	Het
Gm15922	T	C	7: 3,737,494	T243A	probably damaging	Het
Gm3099	A	T	14: 4,000,520	N118I	probably damaging	Het
Gtse1	A	G	15: 85,871,547	T471A	probably benign	Het
Heg1	G	A	16: 33,760,790	A13T	possibly damaging	Het
Ifi209	A	T	1: 173,642,590	Y248F	possibly damaging	Het
Itgb3bp	C	G	4: 99,769,521	E169Q	probably damaging	Het
Kcna2	A	G	3: 107,104,750	T216A	probably benign	Het
Kcnj6	A	T	16: 94,941,183	M10K	probably benign	Het
Krt17	T	G	11: 100,257,337	Q397P	probably benign	Het
Lrtm1	T	A	14: 29,022,118	M181K	probably damaging	Het
Map3k5	A	G	10: 20,099,555	H741R	probably damaging	Het
Mier1	T	A	4: 103,139,402	Y120*	probably null	Het
Mrvi1	T	C	7: 110,899,724	T367A	possibly damaging	Het
Myh14	T	A	7: 44,629,991	T922S	probably benign	Het
Nfkbie	A	T	17: 45,560,141	I240F	possibly damaging	Het
Npas3	C	A	12: 54,069,041	H915Q	probably damaging	Het
Nrg2	A	T	18: 36,045,941	V314E	probably benign	Het
Olfr373	T	A	8: 72,100,346	Y195*	probably null	Het
Pds5a	T	C	5: 65,619,734	N28S	probably damaging	Het
Pkhd1l1	A	T	15: 44,498,482	N517I	possibly damaging	Het
Plekhb1	T	C	7: 100,645,667	Y99C	probably benign	Het
Plpp6	T	C	19: 28,964,217	S73P	probably benign	Het
Polb	T	C	8: 22,639,959	N199S	probably benign	Het
Ppp1r13b	T	C	12: 111,872,406	N13D	possibly damaging	Het
Ptprn2	T	A	12: 117,248,544	V862D	probably damaging	Het
Rassf4	T	C	6: 116,640,317	I242M	probably damaging	Het
Rnf19a	G	A	15: 36,254,452	T320I	probably damaging	Het
Sctr	A	T	1: 120,022,240	E53V	probably damaging	Het
Srcin1	T	C	11: 97,551,693	D103G	probably benign	Het
St3gal3	T	C	4: 117,957,436	Q220R		Het
Stk40	G	A	4: 126,125,690	E86K	probably benign	Het
Tas2r104	T	A	6: 131,685,042	I235F	possibly damaging	Het
Terf2ip	T	C	8: 112,011,648	V56A	possibly damaging	Het
Thsd7b	A	G	1: 130,103,153	N1075S	probably benign	Het
Trak1	A	G	9: 121,416,212	Y51C	probably benign	Het
Trav16n	T	A	14: 53,351,402	V45E	probably benign	Het
Ttc6	T	C	12: 57,576,051	S79P	probably damaging	Het
Unc79	C	T	12: 103,063,190	T484M	probably damaging	Het
Usp49	T	C	17: 47,672,871	V267A	possibly damaging	Het
Vldlr	T	A	19: 27,238,604	D305E	possibly damaging	Het
Vmn1r226	G	T	17: 20,687,749	C81F	probably damaging	Het
Vmn1r73	T	C	7: 11,756,897	V214A	probably damaging	Het
Vmn2r5	T	C	3: 64,504,250	N299S	probably damaging	Het
Vwa3b	T	C	1: 37,164,505	L55S	probably damaging	Het
Zbtb38	A	G	9: 96,687,427	S535P	probably damaging	Het
Zfp329	T	C	7: 12,810,899	I233V	probably damaging	Het
Zfp579	T	A	7: 4,994,583	T110S	probably benign	Het

Other mutations in Wdr90

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00418:Wdr90	APN	17	25849364	missense	probably damaging	1.00
IGL01118:Wdr90	APN	17	25854687	missense	probably damaging	1.00
IGL01964:Wdr90	APN	17	25848409	missense	probably benign
IGL02116:Wdr90	APN	17	25859492	missense	probably benign	0.12
IGL02172:Wdr90	APN	17	25850434	missense	probably benign	0.22
IGL02716:Wdr90	APN	17	25857220	missense	probably damaging	0.96
IGL02961:Wdr90	APN	17	25848675	nonsense	probably null
IGL03229:Wdr90	APN	17	25845463	splice site	probably benign
IGL03367:Wdr90	APN	17	25847791	splice site	probably benign
IGL03098:Wdr90	UTSW	17	25859987	intron	probably benign
R0111:Wdr90	UTSW	17	25848444	splice site	probably benign
R0454:Wdr90	UTSW	17	25860049	missense	probably damaging	0.96
R0457:Wdr90	UTSW	17	25860485	missense	probably benign	0.00
R0488:Wdr90	UTSW	17	25848617	missense	probably damaging	1.00
R0622:Wdr90	UTSW	17	25855658	missense	probably damaging	1.00
R0671:Wdr90	UTSW	17	25846393	missense	probably benign	0.04
R0799:Wdr90	UTSW	17	25860130	missense	probably benign	0.38
R1177:Wdr90	UTSW	17	25846054	missense	possibly damaging	0.85
R1460:Wdr90	UTSW	17	25860448	missense	possibly damaging	0.82
R1468:Wdr90	UTSW	17	25854053	missense	probably damaging	1.00
R1468:Wdr90	UTSW	17	25854053	missense	probably damaging	1.00
R1544:Wdr90	UTSW	17	25849310	missense	possibly damaging	0.69
R2057:Wdr90	UTSW	17	25855199	missense	probably benign	0.05
R2087:Wdr90	UTSW	17	25846603	missense	probably damaging	1.00
R2159:Wdr90	UTSW	17	25851741	missense	probably benign
R2208:Wdr90	UTSW	17	25860388	missense	probably damaging	1.00
R2345:Wdr90	UTSW	17	25859162	missense	probably benign	0.05
R2391:Wdr90	UTSW	17	25851455	missense	probably damaging	1.00
R2394:Wdr90	UTSW	17	25851455	missense	probably damaging	1.00
R2520:Wdr90	UTSW	17	25855352	missense	probably damaging	1.00
R3798:Wdr90	UTSW	17	25850498	missense	probably benign	0.01
R3979:Wdr90	UTSW	17	25859278	missense	probably benign	0.00
R4111:Wdr90	UTSW	17	25849368	missense	possibly damaging	0.94
R4326:Wdr90	UTSW	17	25853731	missense	probably benign	0.25
R4459:Wdr90	UTSW	17	25861002	missense	possibly damaging	0.95
R4697:Wdr90	UTSW	17	25855363	missense	probably benign	0.03
R4735:Wdr90	UTSW	17	25859450	missense	probably benign
R4907:Wdr90	UTSW	17	25860650	intron	probably benign
R5070:Wdr90	UTSW	17	25846333	missense	probably damaging	1.00
R5230:Wdr90	UTSW	17	25855303	missense	probably benign	0.01
R5268:Wdr90	UTSW	17	25850845	missense	probably damaging	1.00
R5287:Wdr90	UTSW	17	25861467	utr 5 prime	probably benign
R5382:Wdr90	UTSW	17	25845598	missense	probably damaging	1.00
R5511:Wdr90	UTSW	17	25845021	unclassified	probably benign
R5545:Wdr90	UTSW	17	25845856	missense	probably damaging	1.00
R5707:Wdr90	UTSW	17	25857192	missense	probably benign	0.00
R5973:Wdr90	UTSW	17	25845133	missense	probably damaging	0.99
R5973:Wdr90	UTSW	17	25846407	missense	probably damaging	1.00
R6385:Wdr90	UTSW	17	25848530	missense	probably damaging	1.00
R6481:Wdr90	UTSW	17	25845911	missense	probably damaging	0.99
R7078:Wdr90	UTSW	17	25849649	missense	probably damaging	1.00
R7214:Wdr90	UTSW	17	25845393	missense	probably benign	0.00
R7288:Wdr90	UTSW	17	25846312	missense	probably benign	0.03
R7309:Wdr90	UTSW	17	25860702	missense	probably benign	0.02
R7391:Wdr90	UTSW	17	25846528	missense	probably benign	0.08
R7622:Wdr90	UTSW	17	25854109	missense	probably benign	0.00
R7646:Wdr90	UTSW	17	25860130	missense	probably benign	0.38
R7772:Wdr90	UTSW	17	25861491	start gained	probably benign
R7779:Wdr90	UTSW	17	25846326	missense	probably damaging	1.00
R7780:Wdr90	UTSW	17	25846326	missense	probably damaging	1.00
R7781:Wdr90	UTSW	17	25846326	missense	probably damaging	1.00
R7782:Wdr90	UTSW	17	25846326	missense	probably damaging	1.00
R7812:Wdr90	UTSW	17	25852558	missense	probably damaging	1.00
R7870:Wdr90	UTSW	17	25860539	missense	probably damaging	0.96
R7911:Wdr90	UTSW	17	25850749	missense	probably benign	0.00
R8126:Wdr90	UTSW	17	25848977	missense	possibly damaging	0.48
R8260:Wdr90	UTSW	17	25845167	missense	probably damaging	1.00
R8315:Wdr90	UTSW	17	25845425	missense	probably benign	0.21
R8919:Wdr90	UTSW	17	25857172	missense
R8938:Wdr90	UTSW	17	25857172	missense
R8940:Wdr90	UTSW	17	25857172	missense
R9038:Wdr90	UTSW	17	25857226	missense
R9079:Wdr90	UTSW	17	25857429	missense
R9401:Wdr90	UTSW	17	25845776
X0064:Wdr90	UTSW	17	25848563	missense	probably damaging	1.00
Z1176:Wdr90	UTSW	17	25860496	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AGGGAACAACAGCTACCTGG -3'
(R):5'- ATTCAGGCTTAGCTGGGAGAG -3'

Sequencing Primer
(F):5'- TGGCCCGACAGCACTAAG -3'
(R):5'- GAGGGCATGACCGTGTTAG -3'

Posted On

2019-06-26