Mutagenetix > Incidental Mutation

Incidental Mutation 'R7305:Herc1'

567245

Institutional Source

Beutler Lab

Gene Symbol

Herc1

Ensembl Gene

ENSMUSG00000038664

Gene Name

HECT and RLD domain containing E3 ubiquitin protein ligase family member 1

Synonyms

tbl, D130015N03Rik, 2810449H11Rik

MMRRC Submission

045407-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7305 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

66257732-66416057 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 66369150 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 452 (D452G)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042824]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000042824
AA Change: D2948G

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000044801
Gene: ENSMUSG00000038664
AA Change: D2948G

Domain	Start	End	E-Value	Type
low complexity region	79	90	N/A	INTRINSIC
low complexity region	136	147	N/A	INTRINSIC
Pfam:RCC1	476	526	5.4e-15	PFAM
Pfam:RCC1_2	513	542	1.3e-9	PFAM
Pfam:RCC1	529	576	5.5e-16	PFAM
Pfam:RCC1	579	629	1.5e-10	PFAM
Pfam:RCC1	632	680	3.6e-9	PFAM
Pfam:RCC1_2	667	696	2.2e-11	PFAM
Pfam:RCC1	683	733	1.2e-14	PFAM
low complexity region	787	807	N/A	INTRINSIC
low complexity region	852	864	N/A	INTRINSIC
low complexity region	1014	1025	N/A	INTRINSIC
low complexity region	1080	1100	N/A	INTRINSIC
low complexity region	1348	1378	N/A	INTRINSIC
low complexity region	1659	1676	N/A	INTRINSIC
low complexity region	1865	1874	N/A	INTRINSIC
low complexity region	2002	2030	N/A	INTRINSIC
SPRY	2067	2188	1.8e-30	SMART
coiled coil region	2251	2280	N/A	INTRINSIC
low complexity region	2410	2423	N/A	INTRINSIC
low complexity region	2613	2629	N/A	INTRINSIC
low complexity region	2633	2648	N/A	INTRINSIC
low complexity region	2650	2667	N/A	INTRINSIC
low complexity region	2736	2749	N/A	INTRINSIC
low complexity region	2882	2896	N/A	INTRINSIC
low complexity region	2924	2935	N/A	INTRINSIC
low complexity region	2971	2987	N/A	INTRINSIC
low complexity region	3045	3051	N/A	INTRINSIC
low complexity region	3168	3186	N/A	INTRINSIC
low complexity region	3191	3213	N/A	INTRINSIC
low complexity region	3364	3379	N/A	INTRINSIC
WD40	3415	3454	1.68e-6	SMART
WD40	3570	3608	3.68e1	SMART
WD40	3613	3652	4.3e-1	SMART
WD40	3657	3702	3.17e-2	SMART
WD40	3734	3773	8.29e-6	SMART
low complexity region	3950	3964	N/A	INTRINSIC
Pfam:RCC1_2	4079	4111	7.3e-9	PFAM
Pfam:RCC1	4098	4147	3.4e-16	PFAM
Pfam:RCC1_2	4134	4163	1.8e-7	PFAM
Pfam:RCC1	4150	4199	7.2e-16	PFAM
Pfam:RCC1	4204	4252	6.1e-12	PFAM
Pfam:RCC1	4255	4304	2.4e-7	PFAM
Pfam:RCC1_2	4291	4320	5.8e-12	PFAM
Pfam:RCC1	4307	4356	8.9e-16	PFAM
Blast:HECTc	4389	4423	2e-11	BLAST
HECTc	4497	4846	8.2e-148	SMART

Predicted Effect

SMART Domains

Protein: ENSMUSP00000119991
Gene: ENSMUSG00000038664
AA Change: D452G

Domain	Start	End	E-Value	Type
low complexity region	118	134	N/A	INTRINSIC
low complexity region	138	153	N/A	INTRINSIC
low complexity region	155	172	N/A	INTRINSIC
low complexity region	241	254	N/A	INTRINSIC
low complexity region	387	401	N/A	INTRINSIC
low complexity region	429	440	N/A	INTRINSIC
low complexity region	469	485	N/A	INTRINSIC
low complexity region	543	549	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gen encodes a member of the HERC protein family. This protein stimulates guanine nucleotide exchange on ARF1 and Rab proteins. This protein may be involved in membrane transport processes. [provided by RefSeq, Mar 2012]
PHENOTYPE: Homozygotes for this spontaneous mutation exhibit an abnormal cerebellar Purkinje cell layer and Purkinje cell degeneration. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 80 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abhd16b	A	T	2: 181,135,209 (GRCm39)	D37V	possibly damaging	Het
Ankhd1	A	G	18: 36,765,258 (GRCm39)	D87G		Het
Ankrd31	A	G	13: 97,015,479 (GRCm39)	S1583G	probably damaging	Het
Ankub1	T	C	3: 57,599,938 (GRCm39)		probably benign	Het
Apba3	A	G	10: 81,107,067 (GRCm39)	D264G	probably damaging	Het
Armh3	A	T	19: 45,880,560 (GRCm39)	M508K	probably benign	Het
Asap1	G	A	15: 64,002,099 (GRCm39)	T404M	probably damaging	Het
Cnot3	A	T	7: 3,648,479 (GRCm39)		probably benign	Het
Cxxc1	A	G	18: 74,352,467 (GRCm39)	Y349C	probably benign	Het
Cyfip1	AGTGT	AGT	7: 55,577,937 (GRCm39)		probably null	Het
Cyp3a57	A	G	5: 145,307,795 (GRCm39)	I184V	probably benign	Het
D130052B06Rik	GTCTACACTGTCCTGCACAGGTGACCCATCTACCCCGTCCTATCCTGGCGACCCATCTACACTGTCCTG	GTCTACACTGTCCTG	11: 33,573,355 (GRCm39)		probably null	Het
Dab1	T	A	4: 104,570,987 (GRCm39)	D210E		Het
Elavl1	G	A	8: 4,375,199 (GRCm39)		probably benign	Het
Emilin1	C	T	5: 31,074,433 (GRCm39)	Q225*	probably null	Het
Eml6	G	T	11: 29,727,258 (GRCm39)	A1288E	probably benign	Het
Eno1	T	C	4: 150,329,796 (GRCm39)		probably null	Het
Eprs1	C	T	1: 185,111,898 (GRCm39)	R303C	probably damaging	Het
Eps15l1	G	A	8: 73,126,878 (GRCm39)	A651V	probably benign	Het
Etl4	A	T	2: 20,714,368 (GRCm39)	I156F	probably damaging	Het
Faim2	A	T	15: 99,411,814 (GRCm39)	I171N	probably damaging	Het
Fam135a	T	A	1: 24,069,939 (GRCm39)	N381I	probably damaging	Het
Fhip1a	G	T	3: 85,637,831 (GRCm39)	P156Q	probably damaging	Het
Gabrg3	T	A	7: 56,384,833 (GRCm39)	M243L	probably benign	Het
Garin2	A	G	12: 78,761,809 (GRCm39)	K158E	possibly damaging	Het
Gm5134	A	G	10: 75,836,233 (GRCm39)	I405V	probably damaging	Het
Gm9376	A	T	14: 118,504,768 (GRCm39)	K67*	probably null	Het
Grm8	A	T	6: 27,761,354 (GRCm39)	I290K	possibly damaging	Het
Hao1	T	A	2: 134,390,121 (GRCm39)	M73L	probably benign	Het
Idh3b	C	A	2: 130,123,413 (GRCm39)	K192N	possibly damaging	Het
Igkv6-23	A	G	6: 70,237,553 (GRCm39)	S63P	probably benign	Het
Itgb2	A	C	10: 77,384,398 (GRCm39)	D173A	probably damaging	Het
Jmjd8	A	T	17: 26,049,301 (GRCm39)	T255S	probably benign	Het
Lamc3	A	C	2: 31,820,714 (GRCm39)	E1243A	probably benign	Het
Map1a	A	G	2: 121,129,939 (GRCm39)	T252A	probably damaging	Het
Mrgpra1	C	A	7: 46,985,203 (GRCm39)	A159S	probably benign	Het
Ndst3	T	C	3: 123,395,131 (GRCm39)	I500V	possibly damaging	Het
Nhsl1	A	G	10: 18,407,434 (GRCm39)	T1523A	possibly damaging	Het
Nr2f1	A	G	13: 78,343,298 (GRCm39)	I322T	probably damaging	Het
Nup210	T	C	6: 91,064,948 (GRCm39)	E184G	probably damaging	Het
Obsl1	C	T	1: 75,470,590 (GRCm39)	W1022*	probably null	Het
Or2l13	T	A	16: 19,306,449 (GRCm39)	I287N	probably damaging	Het
Or4a77	T	A	2: 89,486,846 (GRCm39)	H313L	probably benign	Het
Or52p2	G	T	7: 102,237,162 (GRCm39)	Q263K	possibly damaging	Het
Or5p51	A	T	7: 107,444,572 (GRCm39)	Y123N	probably damaging	Het
Or6c1	A	T	10: 129,518,149 (GRCm39)	I153N	probably damaging	Het
Or6c65	T	A	10: 129,603,720 (GRCm39)	Y118*	probably null	Het
Otulinl	A	G	15: 27,658,319 (GRCm39)	C184R	probably benign	Het
Oxr1	C	T	15: 41,677,004 (GRCm39)	P187L	not run	Het
Parp8	A	G	13: 117,031,461 (GRCm39)	L417P	possibly damaging	Het
Pdia6	A	G	12: 17,324,509 (GRCm39)	Q120R	probably benign	Het
Ppp3cc	T	C	14: 70,478,252 (GRCm39)	N290S	probably benign	Het
Prdm5	C	A	6: 65,808,244 (GRCm39)	S63R	possibly damaging	Het
Prr14l	T	C	5: 32,988,445 (GRCm39)	D350G	probably benign	Het
Pwwp2a	T	C	11: 43,607,878 (GRCm39)	L497S	probably damaging	Het
R3hdm2	A	G	10: 127,312,547 (GRCm39)	N430D	probably benign	Het
Rad51ap2	A	G	12: 11,507,344 (GRCm39)	N422S	possibly damaging	Het
Rbbp8	G	A	18: 11,805,638 (GRCm39)		probably null	Het
Rsf1	GGCGGCGGC	GGCGGCGGCAGCGGCGGC	7: 97,229,125 (GRCm39)		probably benign	Het
Slc22a6	G	A	19: 8,599,522 (GRCm39)		probably null	Het
Slc28a3	T	A	13: 58,714,045 (GRCm39)	E440V	possibly damaging	Het
Slc30a5	A	T	13: 100,947,932 (GRCm39)	I482K	probably damaging	Het
Slco1a1	A	T	6: 141,870,223 (GRCm39)	F305Y	probably damaging	Het
Slco1a8	G	A	6: 141,938,220 (GRCm39)	A253V	probably damaging	Het
Slco4c1	T	C	1: 96,756,690 (GRCm39)	N544S	probably damaging	Het
Smpd4	T	A	16: 17,459,647 (GRCm39)	I656N	probably damaging	Het
Spata31h1	A	T	10: 82,120,953 (GRCm39)	I4019K	probably benign	Het
Taok1	A	T	11: 77,432,500 (GRCm39)	L771*	probably null	Het
Tmem231	T	C	8: 112,641,927 (GRCm39)	D209G	possibly damaging	Het
Tmem25	A	G	9: 44,706,705 (GRCm39)		probably null	Het
Tmem79	T	C	3: 88,240,718 (GRCm39)	T77A	probably benign	Het
Topbp1	A	T	9: 103,205,836 (GRCm39)	T825S	probably damaging	Het
Trpm6	A	T	19: 18,853,455 (GRCm39)	Q1825L	probably benign	Het
Uba1y	T	A	Y: 821,348 (GRCm39)	D110E	probably damaging	Het
Utrn	A	T	10: 12,261,280 (GRCm39)	N3422K	probably benign	Het
Vmn1r219	A	T	13: 23,347,314 (GRCm39)	M168L	probably benign	Het
Vmn2r62	T	C	7: 42,414,235 (GRCm39)	H736R	possibly damaging	Het
Wdr1	T	C	5: 38,697,435 (GRCm39)	H291R	possibly damaging	Het
Zan	T	C	5: 137,413,401 (GRCm39)	T3177A	unknown	Het
Zbtb21	T	C	16: 97,752,495 (GRCm39)	H596R	possibly damaging	Het

Other mutations in Herc1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00092:Herc1	APN	9	66,391,248 (GRCm39)	missense	probably benign	0.02
IGL00159:Herc1	APN	9	66,344,964 (GRCm39)	missense	possibly damaging	0.94
IGL00486:Herc1	APN	9	66,383,402 (GRCm39)	missense	probably benign
IGL00717:Herc1	APN	9	66,392,284 (GRCm39)	missense	probably damaging	1.00
IGL00766:Herc1	APN	9	66,358,023 (GRCm39)	missense	probably damaging	1.00
IGL00776:Herc1	APN	9	66,328,320 (GRCm39)	missense	probably benign
IGL00987:Herc1	APN	9	66,315,334 (GRCm39)	missense	probably benign	0.07
IGL01090:Herc1	APN	9	66,376,457 (GRCm39)	nonsense	probably null
IGL01098:Herc1	APN	9	66,369,204 (GRCm39)	critical splice donor site	probably null
IGL01106:Herc1	APN	9	66,383,720 (GRCm39)	splice site	probably benign
IGL01120:Herc1	APN	9	66,336,162 (GRCm39)	missense	probably benign
IGL01359:Herc1	APN	9	66,346,550 (GRCm39)	missense	probably benign	0.01
IGL01360:Herc1	APN	9	66,390,981 (GRCm39)	missense	probably benign
IGL01364:Herc1	APN	9	66,306,643 (GRCm39)	missense	probably benign	0.00
IGL01470:Herc1	APN	9	66,404,918 (GRCm39)	missense	possibly damaging	0.94
IGL01670:Herc1	APN	9	66,394,342 (GRCm39)	missense	probably damaging	1.00
IGL01825:Herc1	APN	9	66,307,089 (GRCm39)	missense	probably benign	0.00
IGL01903:Herc1	APN	9	66,294,154 (GRCm39)	nonsense	probably null
IGL01988:Herc1	APN	9	66,395,357 (GRCm39)	splice site	probably benign
IGL02074:Herc1	APN	9	66,358,265 (GRCm39)	missense	probably benign
IGL02089:Herc1	APN	9	66,388,151 (GRCm39)	missense	probably damaging	1.00
IGL02177:Herc1	APN	9	66,341,793 (GRCm39)	missense	probably benign
IGL02300:Herc1	APN	9	66,383,645 (GRCm39)	missense	probably benign	0.01
IGL02304:Herc1	APN	9	66,383,696 (GRCm39)	missense	probably benign	0.06
IGL02369:Herc1	APN	9	66,399,293 (GRCm39)	nonsense	probably null
IGL02445:Herc1	APN	9	66,340,764 (GRCm39)	missense	possibly damaging	0.95
IGL02447:Herc1	APN	9	66,404,610 (GRCm39)	missense	possibly damaging	0.59
IGL02549:Herc1	APN	9	66,307,183 (GRCm39)	missense	probably damaging	0.98
IGL02571:Herc1	APN	9	66,341,887 (GRCm39)	splice site	probably benign
IGL02709:Herc1	APN	9	66,404,962 (GRCm39)	missense	probably damaging	0.97
IGL02717:Herc1	APN	9	66,279,203 (GRCm39)	nonsense	probably null
IGL02726:Herc1	APN	9	66,349,270 (GRCm39)	missense	probably benign	0.37
IGL02733:Herc1	APN	9	66,358,274 (GRCm39)	missense	probably benign
IGL02963:Herc1	APN	9	66,296,105 (GRCm39)	missense	probably damaging	0.99
IGL03101:Herc1	APN	9	66,395,279 (GRCm39)	missense	probably benign
IGL03193:Herc1	APN	9	66,309,962 (GRCm39)	missense	probably benign
IGL03203:Herc1	APN	9	66,296,182 (GRCm39)	critical splice donor site	probably null
IGL03216:Herc1	APN	9	66,386,228 (GRCm39)	missense	probably benign	0.06
IGL03282:Herc1	APN	9	66,358,741 (GRCm39)	missense	probably benign	0.05
IGL03295:Herc1	APN	9	66,303,985 (GRCm39)	missense	possibly damaging	0.56
cradle	UTSW	9	66,391,148 (GRCm39)	splice site	probably null
miracles	UTSW	9	66,370,119 (GRCm39)	nonsense	probably null
newton	UTSW	9	66,375,085 (GRCm39)	missense	probably damaging	1.00
R0907_Herc1_362	UTSW	9	66,340,710 (GRCm39)	missense	possibly damaging	0.94
R4427_Herc1_231	UTSW	9	66,403,287 (GRCm39)	missense	probably damaging	1.00
R5026_Herc1_363	UTSW	9	66,393,408 (GRCm39)	missense	probably benign	0.03
stables	UTSW	9	66,386,735 (GRCm39)	missense	probably benign	0.13
strangle	UTSW	9	66,408,470 (GRCm39)	frame shift	probably null
IGL03134:Herc1	UTSW	9	66,341,345 (GRCm39)	critical splice acceptor site	probably benign
PIT4243001:Herc1	UTSW	9	66,279,489 (GRCm39)	missense	probably benign	0.00
PIT4486001:Herc1	UTSW	9	66,279,671 (GRCm39)	missense	probably damaging	1.00
PIT4696001:Herc1	UTSW	9	66,386,291 (GRCm39)	missense	probably damaging	1.00
R0044:Herc1	UTSW	9	66,355,457 (GRCm39)	missense	probably benign	0.04
R0044:Herc1	UTSW	9	66,355,457 (GRCm39)	missense	probably benign	0.04
R0052:Herc1	UTSW	9	66,307,438 (GRCm39)	missense	probably damaging	0.99
R0114:Herc1	UTSW	9	66,369,128 (GRCm39)	missense	probably damaging	0.99
R0129:Herc1	UTSW	9	66,355,357 (GRCm39)	missense	probably damaging	1.00
R0131:Herc1	UTSW	9	66,388,192 (GRCm39)	missense	probably benign	0.00
R0131:Herc1	UTSW	9	66,388,192 (GRCm39)	missense	probably benign	0.00
R0132:Herc1	UTSW	9	66,388,192 (GRCm39)	missense	probably benign	0.00
R0158:Herc1	UTSW	9	66,403,203 (GRCm39)	nonsense	probably null
R0333:Herc1	UTSW	9	66,371,981 (GRCm39)	splice site	probably null
R0384:Herc1	UTSW	9	66,388,332 (GRCm39)	splice site	probably benign
R0419:Herc1	UTSW	9	66,353,356 (GRCm39)	splice site	probably benign
R0453:Herc1	UTSW	9	66,307,054 (GRCm39)	missense	probably benign	0.20
R0458:Herc1	UTSW	9	66,383,663 (GRCm39)	missense	probably benign	0.12
R0490:Herc1	UTSW	9	66,392,281 (GRCm39)	missense	probably damaging	1.00
R0506:Herc1	UTSW	9	66,355,441 (GRCm39)	missense	probably damaging	0.99
R0513:Herc1	UTSW	9	66,352,927 (GRCm39)	missense	possibly damaging	0.96
R0628:Herc1	UTSW	9	66,358,163 (GRCm39)	missense	probably benign	0.35
R0666:Herc1	UTSW	9	66,392,170 (GRCm39)	splice site	probably benign
R0674:Herc1	UTSW	9	66,408,474 (GRCm39)	missense	probably damaging	0.99
R0682:Herc1	UTSW	9	66,389,263 (GRCm39)	missense	possibly damaging	0.95
R0690:Herc1	UTSW	9	66,294,120 (GRCm39)	nonsense	probably null
R0701:Herc1	UTSW	9	66,395,232 (GRCm39)	missense	probably damaging	1.00
R0766:Herc1	UTSW	9	66,412,122 (GRCm39)	missense	probably damaging	1.00
R0850:Herc1	UTSW	9	66,373,952 (GRCm39)	missense	probably damaging	1.00
R0907:Herc1	UTSW	9	66,340,710 (GRCm39)	missense	possibly damaging	0.94
R0972:Herc1	UTSW	9	66,279,427 (GRCm39)	missense	probably damaging	1.00
R0976:Herc1	UTSW	9	66,347,160 (GRCm39)	missense	possibly damaging	0.74
R1027:Herc1	UTSW	9	66,363,250 (GRCm39)	missense	probably benign
R1200:Herc1	UTSW	9	66,393,406 (GRCm39)	missense	probably damaging	1.00
R1226:Herc1	UTSW	9	66,323,545 (GRCm39)	missense	probably benign	0.00
R1364:Herc1	UTSW	9	66,307,375 (GRCm39)	missense	probably damaging	1.00
R1395:Herc1	UTSW	9	66,346,463 (GRCm39)	missense	probably benign	0.13
R1432:Herc1	UTSW	9	66,372,751 (GRCm39)	missense	probably benign	0.13
R1440:Herc1	UTSW	9	66,375,085 (GRCm39)	missense	probably damaging	1.00
R1476:Herc1	UTSW	9	66,415,548 (GRCm39)	missense	probably damaging	1.00
R1590:Herc1	UTSW	9	66,399,235 (GRCm39)	splice site	probably benign
R1634:Herc1	UTSW	9	66,380,820 (GRCm39)	missense	possibly damaging	0.51
R1700:Herc1	UTSW	9	66,357,960 (GRCm39)	splice site	probably null
R1753:Herc1	UTSW	9	66,409,366 (GRCm39)	critical splice donor site	probably null
R1753:Herc1	UTSW	9	66,376,292 (GRCm39)	missense	probably damaging	1.00
R1796:Herc1	UTSW	9	66,296,138 (GRCm39)	nonsense	probably null
R1830:Herc1	UTSW	9	66,404,881 (GRCm39)	missense	possibly damaging	0.95
R1855:Herc1	UTSW	9	66,298,708 (GRCm39)	missense	possibly damaging	0.95
R1866:Herc1	UTSW	9	66,358,073 (GRCm39)	missense	probably damaging	1.00
R1894:Herc1	UTSW	9	66,386,743 (GRCm39)	missense	probably damaging	1.00
R1918:Herc1	UTSW	9	66,383,408 (GRCm39)	splice site	probably null
R1999:Herc1	UTSW	9	66,393,360 (GRCm39)	missense	probably benign	0.07
R2034:Herc1	UTSW	9	66,349,254 (GRCm39)	missense	probably benign	0.01
R2138:Herc1	UTSW	9	66,377,589 (GRCm39)	missense	possibly damaging	0.94
R2186:Herc1	UTSW	9	66,347,183 (GRCm39)	missense	probably benign	0.45
R2192:Herc1	UTSW	9	66,372,688 (GRCm39)	missense	probably damaging	0.99
R2312:Herc1	UTSW	9	66,415,563 (GRCm39)	nonsense	probably null
R2338:Herc1	UTSW	9	66,336,251 (GRCm39)	missense	possibly damaging	0.69
R3035:Herc1	UTSW	9	66,391,217 (GRCm39)	missense	possibly damaging	0.89
R3732:Herc1	UTSW	9	66,352,922 (GRCm39)	missense	probably damaging	1.00
R3732:Herc1	UTSW	9	66,352,922 (GRCm39)	missense	probably damaging	1.00
R3733:Herc1	UTSW	9	66,352,922 (GRCm39)	missense	probably damaging	1.00
R3917:Herc1	UTSW	9	66,341,748 (GRCm39)	missense	possibly damaging	0.94
R3953:Herc1	UTSW	9	66,341,075 (GRCm39)	nonsense	probably null
R4073:Herc1	UTSW	9	66,325,774 (GRCm39)	missense	probably benign	0.12
R4075:Herc1	UTSW	9	66,325,774 (GRCm39)	missense	probably benign	0.12
R4241:Herc1	UTSW	9	66,355,630 (GRCm39)	frame shift	probably null
R4260:Herc1	UTSW	9	66,355,630 (GRCm39)	frame shift	probably null
R4261:Herc1	UTSW	9	66,355,630 (GRCm39)	frame shift	probably null
R4300:Herc1	UTSW	9	66,396,688 (GRCm39)	missense	probably damaging	1.00
R4398:Herc1	UTSW	9	66,386,735 (GRCm39)	missense	probably benign	0.13
R4426:Herc1	UTSW	9	66,403,287 (GRCm39)	missense	probably damaging	1.00
R4427:Herc1	UTSW	9	66,403,287 (GRCm39)	missense	probably damaging	1.00
R4590:Herc1	UTSW	9	66,344,946 (GRCm39)	missense	probably damaging	0.97
R4630:Herc1	UTSW	9	66,340,996 (GRCm39)	splice site	probably null
R4656:Herc1	UTSW	9	66,301,993 (GRCm39)	missense	probably damaging	0.97
R4658:Herc1	UTSW	9	66,386,773 (GRCm39)	missense	possibly damaging	0.50
R4663:Herc1	UTSW	9	66,340,660 (GRCm39)	missense	probably damaging	0.98
R4675:Herc1	UTSW	9	66,298,740 (GRCm39)	missense	probably damaging	1.00
R4678:Herc1	UTSW	9	66,323,551 (GRCm39)	missense	probably benign	0.00
R4754:Herc1	UTSW	9	66,408,488 (GRCm39)	missense	probably benign	0.00
R4766:Herc1	UTSW	9	66,349,211 (GRCm39)	missense	probably benign	0.00
R4792:Herc1	UTSW	9	66,403,266 (GRCm39)	missense	possibly damaging	0.67
R4828:Herc1	UTSW	9	66,404,625 (GRCm39)	splice site	probably null
R4832:Herc1	UTSW	9	66,403,253 (GRCm39)	missense	probably benign	0.11
R4879:Herc1	UTSW	9	66,370,119 (GRCm39)	nonsense	probably null
R4948:Herc1	UTSW	9	66,392,184 (GRCm39)	missense	probably benign
R5021:Herc1	UTSW	9	66,377,608 (GRCm39)	missense	possibly damaging	0.48
R5022:Herc1	UTSW	9	66,377,608 (GRCm39)	missense	possibly damaging	0.48
R5023:Herc1	UTSW	9	66,377,608 (GRCm39)	missense	possibly damaging	0.48
R5024:Herc1	UTSW	9	66,377,608 (GRCm39)	missense	possibly damaging	0.48
R5025:Herc1	UTSW	9	66,377,608 (GRCm39)	missense	possibly damaging	0.48
R5026:Herc1	UTSW	9	66,393,408 (GRCm39)	missense	probably benign	0.03
R5027:Herc1	UTSW	9	66,380,811 (GRCm39)	missense	probably benign	0.01
R5027:Herc1	UTSW	9	66,411,900 (GRCm39)	missense	probably damaging	0.98
R5038:Herc1	UTSW	9	66,383,742 (GRCm39)	intron	probably benign
R5041:Herc1	UTSW	9	66,336,327 (GRCm39)	missense	possibly damaging	0.86
R5053:Herc1	UTSW	9	66,377,608 (GRCm39)	missense	possibly damaging	0.48
R5137:Herc1	UTSW	9	66,355,505 (GRCm39)	missense	probably benign
R5197:Herc1	UTSW	9	66,355,786 (GRCm39)	missense	probably damaging	0.99
R5207:Herc1	UTSW	9	66,307,151 (GRCm39)	nonsense	probably null
R5247:Herc1	UTSW	9	66,341,833 (GRCm39)	missense	probably benign	0.01
R5267:Herc1	UTSW	9	66,369,091 (GRCm39)	missense	probably damaging	1.00
R5274:Herc1	UTSW	9	66,306,691 (GRCm39)	missense	probably benign
R5375:Herc1	UTSW	9	66,375,169 (GRCm39)	missense	probably damaging	0.99
R5401:Herc1	UTSW	9	66,409,338 (GRCm39)	missense	probably damaging	1.00
R5560:Herc1	UTSW	9	66,358,401 (GRCm39)	missense	probably benign	0.02
R5566:Herc1	UTSW	9	66,372,819 (GRCm39)	missense	possibly damaging	0.95
R5577:Herc1	UTSW	9	66,389,263 (GRCm39)	missense	probably damaging	0.99
R5596:Herc1	UTSW	9	66,341,345 (GRCm39)	critical splice acceptor site	probably benign
R5665:Herc1	UTSW	9	66,372,717 (GRCm39)	missense	probably damaging	1.00
R5744:Herc1	UTSW	9	66,415,475 (GRCm39)	missense	probably damaging	1.00
R5802:Herc1	UTSW	9	66,370,160 (GRCm39)	missense	probably damaging	1.00
R5822:Herc1	UTSW	9	66,352,894 (GRCm39)	missense	probably benign	0.00
R5954:Herc1	UTSW	9	66,358,774 (GRCm39)	splice site	probably benign
R5977:Herc1	UTSW	9	66,340,604 (GRCm39)	missense	possibly damaging	0.77
R6022:Herc1	UTSW	9	66,390,967 (GRCm39)	missense	probably damaging	1.00
R6043:Herc1	UTSW	9	66,315,436 (GRCm39)	missense	probably benign
R6046:Herc1	UTSW	9	66,352,831 (GRCm39)	missense	probably damaging	0.99
R6089:Herc1	UTSW	9	66,352,814 (GRCm39)	missense	probably damaging	1.00
R6123:Herc1	UTSW	9	66,404,532 (GRCm39)	missense	probably damaging	0.97
R6155:Herc1	UTSW	9	66,340,705 (GRCm39)	missense	possibly damaging	0.95
R6190:Herc1	UTSW	9	66,283,663 (GRCm39)	missense	possibly damaging	0.56
R6220:Herc1	UTSW	9	66,341,070 (GRCm39)	missense	probably damaging	1.00
R6265:Herc1	UTSW	9	66,279,298 (GRCm39)	missense	probably benign	0.05
R6348:Herc1	UTSW	9	66,395,258 (GRCm39)	missense	possibly damaging	0.77
R6362:Herc1	UTSW	9	66,379,190 (GRCm39)	missense	probably damaging	1.00
R6394:Herc1	UTSW	9	66,302,341 (GRCm39)	missense	probably damaging	0.99
R6434:Herc1	UTSW	9	66,393,464 (GRCm39)	missense	probably damaging	0.99
R6483:Herc1	UTSW	9	66,355,811 (GRCm39)	missense	possibly damaging	0.64
R6607:Herc1	UTSW	9	66,325,849 (GRCm39)	missense	probably benign	0.02
R6633:Herc1	UTSW	9	66,346,534 (GRCm39)	nonsense	probably null
R6634:Herc1	UTSW	9	66,345,026 (GRCm39)	missense	probably benign
R6693:Herc1	UTSW	9	66,386,258 (GRCm39)	missense	probably damaging	0.99
R6695:Herc1	UTSW	9	66,391,148 (GRCm39)	splice site	probably null
R6748:Herc1	UTSW	9	66,408,470 (GRCm39)	frame shift	probably null
R6750:Herc1	UTSW	9	66,408,470 (GRCm39)	frame shift	probably null
R6751:Herc1	UTSW	9	66,408,470 (GRCm39)	frame shift	probably null
R6774:Herc1	UTSW	9	66,408,470 (GRCm39)	frame shift	probably null
R6785:Herc1	UTSW	9	66,408,470 (GRCm39)	frame shift	probably null
R6786:Herc1	UTSW	9	66,408,470 (GRCm39)	frame shift	probably null
R6856:Herc1	UTSW	9	66,305,180 (GRCm39)	missense	probably benign	0.05
R6966:Herc1	UTSW	9	66,318,347 (GRCm39)	missense	probably benign	0.07
R7020:Herc1	UTSW	9	66,393,360 (GRCm39)	missense	probably benign	0.07
R7109:Herc1	UTSW	9	66,389,171 (GRCm39)	missense	probably benign	0.03
R7122:Herc1	UTSW	9	66,307,056 (GRCm39)	missense	possibly damaging	0.69
R7209:Herc1	UTSW	9	66,292,314 (GRCm39)	missense	possibly damaging	0.95
R7222:Herc1	UTSW	9	66,374,781 (GRCm39)	missense	probably damaging	0.98
R7303:Herc1	UTSW	9	66,358,098 (GRCm39)	missense	possibly damaging	0.93
R7438:Herc1	UTSW	9	66,302,038 (GRCm39)	missense	probably benign	0.00
R7535:Herc1	UTSW	9	66,382,135 (GRCm39)	missense	probably damaging	1.00
R7585:Herc1	UTSW	9	66,352,829 (GRCm39)	missense	probably damaging	1.00
R7603:Herc1	UTSW	9	66,358,665 (GRCm39)	nonsense	probably null
R7670:Herc1	UTSW	9	66,323,629 (GRCm39)	missense	probably damaging	0.99
R7705:Herc1	UTSW	9	66,347,116 (GRCm39)	missense	possibly damaging	0.86
R7723:Herc1	UTSW	9	66,279,158 (GRCm39)	missense	probably benign	0.24
R7730:Herc1	UTSW	9	66,400,472 (GRCm39)	small deletion	probably benign
R7880:Herc1	UTSW	9	66,415,506 (GRCm39)	missense	probably damaging	0.99
R7958:Herc1	UTSW	9	66,393,475 (GRCm39)	missense	probably damaging	1.00
R7976:Herc1	UTSW	9	66,341,552 (GRCm39)	missense	possibly damaging	0.94
R8006:Herc1	UTSW	9	66,352,842 (GRCm39)	nonsense	probably null
R8084:Herc1	UTSW	9	66,383,217 (GRCm39)	missense	probably benign	0.45
R8094:Herc1	UTSW	9	66,400,462 (GRCm39)	missense	probably damaging	0.98
R8099:Herc1	UTSW	9	66,279,422 (GRCm39)	missense	probably damaging	1.00
R8151:Herc1	UTSW	9	66,341,073 (GRCm39)	missense	probably damaging	0.98
R8159:Herc1	UTSW	9	66,369,003 (GRCm39)	missense	probably null
R8190:Herc1	UTSW	9	66,325,733 (GRCm39)	missense	probably benign	0.00
R8213:Herc1	UTSW	9	66,358,170 (GRCm39)	missense	probably damaging	0.99
R8230:Herc1	UTSW	9	66,377,598 (GRCm39)	missense	probably damaging	0.99
R8265:Herc1	UTSW	9	66,293,986 (GRCm39)	nonsense	probably null
R8270:Herc1	UTSW	9	66,395,232 (GRCm39)	missense	probably damaging	1.00
R8353:Herc1	UTSW	9	66,415,571 (GRCm39)	missense	possibly damaging	0.88
R8423:Herc1	UTSW	9	66,415,442 (GRCm39)	missense	probably damaging	0.99
R8506:Herc1	UTSW	9	66,380,863 (GRCm39)	missense	possibly damaging	0.52
R8523:Herc1	UTSW	9	66,358,224 (GRCm39)	missense	probably benign
R8530:Herc1	UTSW	9	66,325,910 (GRCm39)	missense	probably benign
R8545:Herc1	UTSW	9	66,279,257 (GRCm39)	nonsense	probably null
R8682:Herc1	UTSW	9	66,370,130 (GRCm39)	missense
R8720:Herc1	UTSW	9	66,389,105 (GRCm39)	missense	probably benign	0.38
R8792:Herc1	UTSW	9	66,372,768 (GRCm39)	missense	probably damaging	1.00
R8915:Herc1	UTSW	9	66,318,456 (GRCm39)	missense	probably damaging	1.00
R8964:Herc1	UTSW	9	66,352,872 (GRCm39)	missense	probably damaging	1.00
R9056:Herc1	UTSW	9	66,380,782 (GRCm39)	missense	probably benign	0.10
R9158:Herc1	UTSW	9	66,376,400 (GRCm39)	missense	probably benign	0.00
R9167:Herc1	UTSW	9	66,411,900 (GRCm39)	missense	possibly damaging	0.75
R9192:Herc1	UTSW	9	66,321,413 (GRCm39)	missense	probably benign	0.35
R9252:Herc1	UTSW	9	66,309,834 (GRCm39)	missense	probably damaging	1.00
R9260:Herc1	UTSW	9	66,325,691 (GRCm39)	nonsense	probably null
R9261:Herc1	UTSW	9	66,412,129 (GRCm39)	missense	probably damaging	0.98
R9430:Herc1	UTSW	9	66,325,785 (GRCm39)	nonsense	probably null
R9519:Herc1	UTSW	9	66,307,356 (GRCm39)	missense	probably damaging	0.97
R9563:Herc1	UTSW	9	66,294,193 (GRCm39)	critical splice donor site	probably null
R9589:Herc1	UTSW	9	66,372,840 (GRCm39)	missense	possibly damaging	0.95
R9600:Herc1	UTSW	9	66,304,594 (GRCm39)	missense	possibly damaging	0.95
R9659:Herc1	UTSW	9	66,307,185 (GRCm39)	missense	probably benign	0.03
R9740:Herc1	UTSW	9	66,355,796 (GRCm39)	missense	probably damaging	1.00
R9774:Herc1	UTSW	9	66,372,032 (GRCm39)	missense	probably null
R9781:Herc1	UTSW	9	66,280,004 (GRCm39)	missense	probably benign
R9788:Herc1	UTSW	9	66,307,185 (GRCm39)	missense	probably benign	0.03
RF023:Herc1	UTSW	9	66,365,616 (GRCm39)	missense
X0011:Herc1	UTSW	9	66,307,441 (GRCm39)	missense	probably benign	0.28
X0067:Herc1	UTSW	9	66,355,806 (GRCm39)	missense	probably benign	0.03
Z1176:Herc1	UTSW	9	66,341,858 (GRCm39)	missense	probably benign
Z1177:Herc1	UTSW	9	66,379,193 (GRCm39)	missense	probably damaging	0.99
Z1177:Herc1	UTSW	9	66,365,707 (GRCm39)	missense	probably null

Predicted Primers

PCR Primer
(F):5'- GTTCTGAGCGGGGTTATACC -3'
(R):5'- TCATCTAGTCTGGGGAGAGAAG -3'

Sequencing Primer
(F):5'- GTTATACCGCTCTGTGCAGGC -3'
(R):5'- TGGGTATCAACGCAATGCTC -3'

Posted On

2019-06-26