Mutagenetix > Incidental Mutations

Incidental Mutation 'R7309:Wdr90'

567464

Institutional Source

Beutler Lab

Gene Symbol

Wdr90

Ensembl Gene

ENSMUSG00000073434

Gene Name

WD repeat domain 90

Synonyms

3230401M21Rik

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.101) question?

Stock #

R7309 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

25844771-25861501 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 25860702 bp

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 190 (D190G)

Ref Sequence

ENSEMBL: ENSMUSP00000078426 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000026828] [ENSMUST00000079461] [ENSMUST00000176923]

Predicted Effect

probably benign
Transcript: ENSMUST00000026828

SMART Domains

Protein: ENSMUSP00000026828
Gene: ENSMUSG00000025732

Domain	Start	End	E-Value	Type
Pfam:FAM195	59	158	8.3e-33	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000079461
AA Change: D190G

PolyPhen 2 Score 0.020 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000078426
Gene: ENSMUSG00000073434
AA Change: D190G

Domain	Start	End	E-Value	Type
low complexity region	32	53	N/A	INTRINSIC
Pfam:DUF667	60	183	5e-26	PFAM
Pfam:DUF667	210	271	3.7e-9	PFAM
low complexity region	381	396	N/A	INTRINSIC
WD40	478	519	5.94e0	SMART
WD40	522	565	3.2e0	SMART
WD40	572	612	3.3e1	SMART
WD40	687	725	1.15e1	SMART
WD40	728	766	5.75e-1	SMART
WD40	768	808	9.24e-4	SMART
WD40	811	850	4.13e0	SMART
WD40	853	892	4.62e-1	SMART
WD40	950	993	1.07e1	SMART
WD40	996	1035	5.75e-1	SMART
WD40	1040	1077	1.58e-2	SMART
WD40	1290	1334	5.23e-3	SMART
WD40	1337	1378	1.27e-1	SMART
WD40	1384	1419	1.83e2	SMART
WD40	1422	1469	3.08e0	SMART
WD40	1472	1509	9.9e0	SMART
WD40	1568	1607	9.02e-7	SMART
WD40	1610	1655	5.75e-1	SMART
WD40	1659	1697	2.98e-1	SMART
WD40	1700	1749	6.14e1	SMART
WD40	1850	1888	1.92e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000176678

Predicted Effect

probably benign
Transcript: ENSMUST00000176923

SMART Domains

Protein: ENSMUSP00000135420
Gene: ENSMUSG00000073434

Domain	Start	End	E-Value	Type
low complexity region	32	53	N/A	INTRINSIC
Pfam:DUF667	60	252	5.1e-14	PFAM
low complexity region	363	378	N/A	INTRINSIC
WD40	460	501	5.94e0	SMART
WD40	504	547	3.2e0	SMART
WD40	554	594	3.3e1	SMART
WD40	669	707	1.15e1	SMART
WD40	710	748	5.75e-1	SMART
WD40	750	790	9.24e-4	SMART
WD40	793	832	4.13e0	SMART
WD40	835	874	4.62e-1	SMART
WD40	932	975	1.07e1	SMART
WD40	978	1017	5.75e-1	SMART
WD40	1022	1059	1.58e-2	SMART
WD40	1272	1316	5.23e-3	SMART
WD40	1319	1360	1.27e-1	SMART
WD40	1366	1401	1.83e2	SMART
WD40	1404	1451	3.08e0	SMART
WD40	1454	1491	9.9e0	SMART
WD40	1550	1589	9.02e-7	SMART
WD40	1592	1637	5.75e-1	SMART
WD40	1641	1679	2.98e-1	SMART
WD40	1682	1731	6.14e1	SMART
WD40	1832	1870	1.92e-1	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

94% (47/50)

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930522L14Rik	A	C	5: 109,736,953	H346Q	probably damaging	Het
4932414N04Rik	A	T	2: 68,716,186	I71L	probably benign	Het
Anapc1	A	G	2: 128,674,684	S377P	probably damaging	Het
Cldnd2	A	G	7: 43,441,709	T22A	possibly damaging	Het
Comp	A	T	8: 70,373,678		probably null	Het
Cop1	A	G	1: 159,306,625	K446E	probably damaging	Het
Cox18	T	C	5: 90,215,058	T314A	possibly damaging	Het
Csrp3	A	G	7: 48,835,569	V60A	probably benign	Het
Dnah8	A	T	17: 30,875,014	Y4694F	probably damaging	Het
Dnm1l	A	G	16: 16,321,646	Y493H	probably damaging	Het
Esf1	A	T	2: 140,125,091		probably null	Het
Fam187a	T	A	11: 102,886,006	V212E	probably damaging	Het
Fign	A	T	2: 63,979,957	M323K	possibly damaging	Het
Foxf2	A	G	13: 31,626,513	K145R	probably damaging	Het
Fxyd5	T	C	7: 31,035,404	N133D	probably benign	Het
Hnrnpdl	A	T	5: 100,037,623	L240*	probably null	Het
Kcna7	T	G	7: 45,409,255	F322C	probably damaging	Het
Kcnj9	A	G	1: 172,326,258	C100R	probably damaging	Het
Lrrc14b	C	A	13: 74,363,202	C253F	probably benign	Het
Map3k11	T	C	19: 5,690,458	V71A	probably damaging	Het
Med13	C	A	11: 86,291,062	M1315I	probably benign	Het
Mettl24	T	C	10: 40,810,500	V291A	probably benign	Het
Miox	G	T	15: 89,336,049	C148F	probably damaging	Het
Mpdz	A	T	4: 81,381,958		probably null	Het
Mthfsd	G	A	8: 121,108,331		probably benign	Het
Myh15	G	A	16: 49,096,465	A383T	probably benign	Het
Nlrc5	A	G	8: 94,474,042	H117R	probably benign	Het
Ntrk1	A	T	3: 87,795,077	M23K	probably benign	Het
Olfr140	T	A	2: 90,051,457	N289I	probably damaging	Het
Olfr297	T	A	7: 86,527,141	L128H	probably damaging	Het
Olfr935	T	A	9: 38,995,280	S52C	probably damaging	Het
Pkd1l3	A	G	8: 109,648,261		probably null	Het
Plcz1	A	T	6: 140,023,156	D185E	probably damaging	Het
Plekhg5	C	A	4: 152,112,528	Q757K	possibly damaging	Het
Prr23a2	T	A	9: 98,856,974	D128E	probably benign	Het
Rsf1	CGGCGGCGG	CGGCGGCGGGGGCGGCGG	7: 97,579,911		probably benign	Het
Sgsm1	A	T	5: 113,268,846		probably null	Het
Sh3bp5	C	T	14: 31,378,289	V221M	probably benign	Het
Slc25a27	A	T	17: 43,664,192	D59E	probably benign	Het
Slc35e1	G	C	8: 72,492,514	R25G	unknown	Het
Slc4a4	T	C	5: 89,170,751	V626A	probably benign	Het
Slfn5	T	C	11: 82,956,703	L138P	probably damaging	Het
Stat1	A	G	1: 52,126,621		probably null	Het
Tnks2	G	T	19: 36,852,536	A206S	probably damaging	Het
Trav7-1	C	A	14: 52,655,064	Q25K	probably benign	Het
Ttn	A	G	2: 76,898,326	M5470T	unknown	Het
Vps35	A	T	8: 85,274,967	D407E	probably benign	Het
Wdr93	T	A	7: 79,773,355	F456I	possibly damaging	Het
Wdr95	A	G	5: 149,606,293	E675G	probably benign	Het

Other mutations in Wdr90

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00418:Wdr90	APN	17	25849364	missense	probably damaging	1.00
IGL01118:Wdr90	APN	17	25854687	missense	probably damaging	1.00
IGL01964:Wdr90	APN	17	25848409	missense	probably benign
IGL02116:Wdr90	APN	17	25859492	missense	probably benign	0.12
IGL02172:Wdr90	APN	17	25850434	missense	probably benign	0.22
IGL02716:Wdr90	APN	17	25857220	missense	probably damaging	0.96
IGL02961:Wdr90	APN	17	25848675	nonsense	probably null
IGL03229:Wdr90	APN	17	25845463	splice site	probably benign
IGL03367:Wdr90	APN	17	25847791	splice site	probably benign
IGL03098:Wdr90	UTSW	17	25859987	intron	probably benign
R0111:Wdr90	UTSW	17	25848444	splice site	probably benign
R0454:Wdr90	UTSW	17	25860049	missense	probably damaging	0.96
R0457:Wdr90	UTSW	17	25860485	missense	probably benign	0.00
R0488:Wdr90	UTSW	17	25848617	missense	probably damaging	1.00
R0622:Wdr90	UTSW	17	25855658	missense	probably damaging	1.00
R0671:Wdr90	UTSW	17	25846393	missense	probably benign	0.04
R0799:Wdr90	UTSW	17	25860130	missense	probably benign	0.38
R1177:Wdr90	UTSW	17	25846054	missense	possibly damaging	0.85
R1460:Wdr90	UTSW	17	25860448	missense	possibly damaging	0.82
R1468:Wdr90	UTSW	17	25854053	missense	probably damaging	1.00
R1468:Wdr90	UTSW	17	25854053	missense	probably damaging	1.00
R1544:Wdr90	UTSW	17	25849310	missense	possibly damaging	0.69
R2057:Wdr90	UTSW	17	25855199	missense	probably benign	0.05
R2087:Wdr90	UTSW	17	25846603	missense	probably damaging	1.00
R2159:Wdr90	UTSW	17	25851741	missense	probably benign
R2208:Wdr90	UTSW	17	25860388	missense	probably damaging	1.00
R2345:Wdr90	UTSW	17	25859162	missense	probably benign	0.05
R2391:Wdr90	UTSW	17	25851455	missense	probably damaging	1.00
R2394:Wdr90	UTSW	17	25851455	missense	probably damaging	1.00
R2520:Wdr90	UTSW	17	25855352	missense	probably damaging	1.00
R3798:Wdr90	UTSW	17	25850498	missense	probably benign	0.01
R3979:Wdr90	UTSW	17	25859278	missense	probably benign	0.00
R4111:Wdr90	UTSW	17	25849368	missense	possibly damaging	0.94
R4326:Wdr90	UTSW	17	25853731	missense	probably benign	0.25
R4459:Wdr90	UTSW	17	25861002	missense	possibly damaging	0.95
R4697:Wdr90	UTSW	17	25855363	missense	probably benign	0.03
R4735:Wdr90	UTSW	17	25859450	missense	probably benign
R4907:Wdr90	UTSW	17	25860650	intron	probably benign
R5070:Wdr90	UTSW	17	25846333	missense	probably damaging	1.00
R5230:Wdr90	UTSW	17	25855303	missense	probably benign	0.01
R5268:Wdr90	UTSW	17	25850845	missense	probably damaging	1.00
R5287:Wdr90	UTSW	17	25861467	utr 5 prime	probably benign
R5382:Wdr90	UTSW	17	25845598	missense	probably damaging	1.00
R5511:Wdr90	UTSW	17	25845021	unclassified	probably benign
R5545:Wdr90	UTSW	17	25845856	missense	probably damaging	1.00
R5707:Wdr90	UTSW	17	25857192	missense	probably benign	0.00
R5973:Wdr90	UTSW	17	25845133	missense	probably damaging	0.99
R5973:Wdr90	UTSW	17	25846407	missense	probably damaging	1.00
R6385:Wdr90	UTSW	17	25848530	missense	probably damaging	1.00
R6481:Wdr90	UTSW	17	25845911	missense	probably damaging	0.99
R7078:Wdr90	UTSW	17	25849649	missense	probably damaging	1.00
R7214:Wdr90	UTSW	17	25845393	missense	probably benign	0.00
R7288:Wdr90	UTSW	17	25846312	missense	probably benign	0.03
R7304:Wdr90	UTSW	17	25851506	missense	probably benign	0.10
R7391:Wdr90	UTSW	17	25846528	missense	probably benign	0.08
R7622:Wdr90	UTSW	17	25854109	missense	probably benign	0.00
R7646:Wdr90	UTSW	17	25860130	missense	probably benign	0.38
R7772:Wdr90	UTSW	17	25861491	start gained	probably benign
R7779:Wdr90	UTSW	17	25846326	missense	probably damaging	1.00
R7780:Wdr90	UTSW	17	25846326	missense	probably damaging	1.00
R7781:Wdr90	UTSW	17	25846326	missense	probably damaging	1.00
R7782:Wdr90	UTSW	17	25846326	missense	probably damaging	1.00
R7812:Wdr90	UTSW	17	25852558	missense	probably damaging	1.00
R7870:Wdr90	UTSW	17	25860539	missense	probably damaging	0.96
R7911:Wdr90	UTSW	17	25850749	missense	probably benign	0.00
R8126:Wdr90	UTSW	17	25848977	missense	possibly damaging	0.48
R8260:Wdr90	UTSW	17	25845167	missense	probably damaging	1.00
R8315:Wdr90	UTSW	17	25845425	missense	probably benign	0.21
X0064:Wdr90	UTSW	17	25848563	missense	probably damaging	1.00
Z1176:Wdr90	UTSW	17	25860496	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- ACGAGGAAGAGCTACACCTG -3'
(R):5'- GCACTTTGTCATCCACCTGG -3'

Sequencing Primer
(F):5'- GAAGAGCTACACCTGCCAGG -3'
(R):5'- AGGTGCCCAGCTTGGAAG -3'

Posted On

2019-06-26