Mutagenetix > Incidental Mutation

Incidental Mutation 'R7311:Treh'

567593

Institutional Source

Beutler Lab

Gene Symbol

Treh

Ensembl Gene

ENSMUSG00000032098

Gene Name

trehalase (brush-border membrane glycoprotein)

Synonyms

2210412M19Rik

MMRRC Submission

045367-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.214) question?

Stock #

R7311 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

44584530-44597602 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 44597245 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 555 (T555A)

Ref Sequence

ENSEMBL: ENSMUSP00000034609 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000034609] [ENSMUST00000034611] [ENSMUST00000071219] [ENSMUST00000134465] [ENSMUST00000135436] [ENSMUST00000138356] [ENSMUST00000139389] [ENSMUST00000144251] [ENSMUST00000147495] [ENSMUST00000150822] [ENSMUST00000156918]

AlphaFold

Q9JLT2

Predicted Effect

probably benign
Transcript: ENSMUST00000034609
AA Change: T555A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000034609
Gene: ENSMUSG00000032098
AA Change: T555A

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
Pfam:Trehalase	43	548	2.3e-185	PFAM
low complexity region	565	575	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000034611

SMART Domains

Protein: ENSMUSP00000034611
Gene: ENSMUSG00000048537

Domain	Start	End	E-Value	Type
PDB:2EH0\|A	40	139	3e-10	PDB
Blast:FHA	63	110	6e-21	BLAST
low complexity region	181	192	N/A	INTRINSIC
low complexity region	252	273	N/A	INTRINSIC
low complexity region	296	316	N/A	INTRINSIC
internal_repeat_1	321	354	5.01e-5	PROSPERO
internal_repeat_1	401	449	5.01e-5	PROSPERO
low complexity region	459	477	N/A	INTRINSIC
low complexity region	590	617	N/A	INTRINSIC
low complexity region	694	707	N/A	INTRINSIC
coiled coil region	715	798	N/A	INTRINSIC
low complexity region	819	829	N/A	INTRINSIC
coiled coil region	865	904	N/A	INTRINSIC
low complexity region	943	961	N/A	INTRINSIC
low complexity region	976	997	N/A	INTRINSIC
low complexity region	1055	1069	N/A	INTRINSIC
low complexity region	1103	1111	N/A	INTRINSIC
coiled coil region	1150	1219	N/A	INTRINSIC
PH	1262	1366	1.31e-17	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000071219
AA Change: T520A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000071206
Gene: ENSMUSG00000032098
AA Change: T520A

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
Pfam:Trehalase	134	513	7e-145	PFAM
low complexity region	530	540	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000128326

SMART Domains

Protein: ENSMUSP00000119966
Gene: ENSMUSG00000048537

Domain	Start	End	E-Value	Type
low complexity region	1	12	N/A	INTRINSIC
low complexity region	83	110	N/A	INTRINSIC
low complexity region	187	200	N/A	INTRINSIC
coiled coil region	207	290	N/A	INTRINSIC
low complexity region	312	322	N/A	INTRINSIC
coiled coil region	357	396	N/A	INTRINSIC
low complexity region	422	443	N/A	INTRINSIC
low complexity region	493	506	N/A	INTRINSIC
low complexity region	516	530	N/A	INTRINSIC
low complexity region	564	572	N/A	INTRINSIC
coiled coil region	610	679	N/A	INTRINSIC
PH	723	827	1.31e-17	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000134465

SMART Domains

Protein: ENSMUSP00000117395
Gene: ENSMUSG00000048537

Domain	Start	End	E-Value	Type
PDB:2EH0\|A	40	139	3e-10	PDB
Blast:FHA	63	110	8e-21	BLAST
low complexity region	181	192	N/A	INTRINSIC
low complexity region	252	273	N/A	INTRINSIC
low complexity region	296	316	N/A	INTRINSIC
internal_repeat_1	321	354	6.75e-5	PROSPERO
internal_repeat_1	401	449	6.75e-5	PROSPERO
low complexity region	459	477	N/A	INTRINSIC
low complexity region	590	617	N/A	INTRINSIC
low complexity region	694	707	N/A	INTRINSIC
coiled coil region	715	798	N/A	INTRINSIC
low complexity region	819	829	N/A	INTRINSIC
coiled coil region	865	904	N/A	INTRINSIC
low complexity region	929	950	N/A	INTRINSIC
low complexity region	1008	1022	N/A	INTRINSIC
low complexity region	1056	1064	N/A	INTRINSIC
coiled coil region	1103	1172	N/A	INTRINSIC
PH	1215	1319	1.31e-17	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000135436

SMART Domains

Protein: ENSMUSP00000120023
Gene: ENSMUSG00000048537

Domain	Start	End	E-Value	Type
low complexity region	67	85	N/A	INTRINSIC
low complexity region	100	121	N/A	INTRINSIC
low complexity region	179	193	N/A	INTRINSIC
low complexity region	227	235	N/A	INTRINSIC
coiled coil region	274	343	N/A	INTRINSIC
PH	386	490	1.31e-17	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000138356

SMART Domains

Protein: ENSMUSP00000120208
Gene: ENSMUSG00000048537

Domain	Start	End	E-Value	Type
PDB:2EH0\|A	40	139	4e-10	PDB
Blast:FHA	63	110	6e-21	BLAST
low complexity region	181	192	N/A	INTRINSIC
low complexity region	252	273	N/A	INTRINSIC
low complexity region	296	316	N/A	INTRINSIC
internal_repeat_1	321	354	4.93e-5	PROSPERO
internal_repeat_1	401	449	4.93e-5	PROSPERO
low complexity region	459	477	N/A	INTRINSIC
low complexity region	590	617	N/A	INTRINSIC
low complexity region	694	707	N/A	INTRINSIC
coiled coil region	715	798	N/A	INTRINSIC
low complexity region	819	829	N/A	INTRINSIC
coiled coil region	865	904	N/A	INTRINSIC
low complexity region	931	948	N/A	INTRINSIC
low complexity region	999	1017	N/A	INTRINSIC
low complexity region	1032	1053	N/A	INTRINSIC
low complexity region	1111	1125	N/A	INTRINSIC
low complexity region	1159	1167	N/A	INTRINSIC
coiled coil region	1206	1286	N/A	INTRINSIC
PH	1329	1444	6.01e-17	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000139389

SMART Domains

Protein: ENSMUSP00000120671
Gene: ENSMUSG00000032098

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
Pfam:Trehalase	43	141	1.3e-21	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000144251

SMART Domains

Protein: ENSMUSP00000114773
Gene: ENSMUSG00000048537

Domain	Start	End	E-Value	Type
low complexity region	11	24	N/A	INTRINSIC
coiled coil region	32	115	N/A	INTRINSIC
coiled coil region	146	174	N/A	INTRINSIC
low complexity region	179	189	N/A	INTRINSIC
coiled coil region	225	264	N/A	INTRINSIC
low complexity region	289	310	N/A	INTRINSIC
low complexity region	368	382	N/A	INTRINSIC
low complexity region	416	424	N/A	INTRINSIC
coiled coil region	463	532	N/A	INTRINSIC
PH	575	679	1.31e-17	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000147495

SMART Domains

Protein: ENSMUSP00000122661
Gene: ENSMUSG00000048537

Domain	Start	End	E-Value	Type
PDB:2EH0\|A	40	139	4e-10	PDB
Blast:FHA	63	110	6e-21	BLAST
low complexity region	181	192	N/A	INTRINSIC
low complexity region	252	273	N/A	INTRINSIC
low complexity region	296	316	N/A	INTRINSIC
internal_repeat_1	321	354	5e-5	PROSPERO
internal_repeat_1	401	449	5e-5	PROSPERO
low complexity region	459	477	N/A	INTRINSIC
low complexity region	590	617	N/A	INTRINSIC
low complexity region	694	707	N/A	INTRINSIC
coiled coil region	715	798	N/A	INTRINSIC
low complexity region	819	829	N/A	INTRINSIC
coiled coil region	865	904	N/A	INTRINSIC
low complexity region	943	961	N/A	INTRINSIC
low complexity region	976	997	N/A	INTRINSIC
low complexity region	1055	1069	N/A	INTRINSIC
low complexity region	1103	1111	N/A	INTRINSIC
coiled coil region	1150	1219	N/A	INTRINSIC
PH	1262	1377	6.01e-17	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000148344

SMART Domains

Protein: ENSMUSP00000121809
Gene: ENSMUSG00000048537

Domain	Start	End	E-Value	Type
low complexity region	4	19	N/A	INTRINSIC
low complexity region	41	61	N/A	INTRINSIC
internal_repeat_1	66	99	6.7e-6	PROSPERO
internal_repeat_1	146	194	6.7e-6	PROSPERO
low complexity region	204	222	N/A	INTRINSIC
low complexity region	335	362	N/A	INTRINSIC
low complexity region	439	452	N/A	INTRINSIC
coiled coil region	459	542	N/A	INTRINSIC
low complexity region	564	574	N/A	INTRINSIC
coiled coil region	609	648	N/A	INTRINSIC
low complexity region	688	706	N/A	INTRINSIC
low complexity region	721	742	N/A	INTRINSIC
low complexity region	792	805	N/A	INTRINSIC
low complexity region	815	829	N/A	INTRINSIC
low complexity region	863	871	N/A	INTRINSIC
coiled coil region	909	978	N/A	INTRINSIC
PH	1022	1126	1.31e-17	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000150822

SMART Domains

Protein: ENSMUSP00000123144
Gene: ENSMUSG00000032098

Domain	Start	End	E-Value	Type
Pfam:Trehalase	1	121	3.1e-32	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000156918

SMART Domains

Protein: ENSMUSP00000120092
Gene: ENSMUSG00000048537

Domain	Start	End	E-Value	Type
low complexity region	11	24	N/A	INTRINSIC
coiled coil region	32	115	N/A	INTRINSIC
low complexity region	136	146	N/A	INTRINSIC
coiled coil region	182	221	N/A	INTRINSIC
low complexity region	246	267	N/A	INTRINSIC
low complexity region	325	339	N/A	INTRINSIC
low complexity region	373	381	N/A	INTRINSIC
coiled coil region	420	489	N/A	INTRINSIC
PH	532	636	1.31e-17	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene belongs to the alpha-glucosidase family, whose members encode enzymes that carry out hydrolysis of alpha-glucoside bonds of a variety of carbohydrates. The enzyme encoded by this gene uses the disaccharide trehalose as a highly specific substrate and converts it into two glucose molecules. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2013]
PHENOTYPE: Mice homozygous for a knock-out allele fail to exhibit a rapid increase in blood glucose levels following oral trehalose administration. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 83 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700001K19Rik	A	G	12: 110,635,184 (GRCm39)	V118A	probably benign	Het
Accsl	C	T	2: 93,696,160 (GRCm39)	G146D	possibly damaging	Het
Acsm1	A	T	7: 119,237,305 (GRCm39)	H206L	probably damaging	Het
Acta2	A	G	19: 34,219,186 (GRCm39)	Y339H	probably damaging	Het
Adcy2	T	C	13: 68,779,073 (GRCm39)	D989G	probably damaging	Het
Ahnak	G	A	19: 8,979,507 (GRCm39)	A264T	probably benign	Het
Ahnak	A	G	19: 8,987,191 (GRCm39)	D2825G	possibly damaging	Het
Akt1	G	T	12: 112,623,587 (GRCm39)	T291K	probably damaging	Het
Arhgap24	A	G	5: 103,040,551 (GRCm39)	D589G	probably damaging	Het
B4galnt3	G	A	6: 120,192,396 (GRCm39)	Q447*	probably null	Het
BC024063	C	T	10: 81,945,993 (GRCm39)	R538C	possibly damaging	Het
Bod1l	A	G	5: 41,951,676 (GRCm39)	S2912P	possibly damaging	Het
Bysl	A	G	17: 47,912,710 (GRCm39)	V360A	possibly damaging	Het
C1qb	A	G	4: 136,607,877 (GRCm39)	V162A	possibly damaging	Het
Ccdc57	G	A	11: 120,764,567 (GRCm39)	P736L	probably benign	Het
Cep290	T	A	10: 100,373,580 (GRCm39)	F1287I	probably damaging	Het
Cntn1	T	C	15: 92,130,156 (GRCm39)		probably null	Het
Col6a3	C	T	1: 90,750,013 (GRCm39)	G274R	probably damaging	Het
Cplane1	G	T	15: 8,210,399 (GRCm39)	V291F	probably damaging	Het
Cyb5r4	T	C	9: 86,937,835 (GRCm39)	C285R	probably damaging	Het
Cyp4f39	G	A	17: 32,708,629 (GRCm39)	C392Y	probably damaging	Het
Dcun1d3	A	T	7: 119,458,734 (GRCm39)	D100E	probably damaging	Het
Dhx58	A	T	11: 100,588,997 (GRCm39)	D516E	probably benign	Het
Dock5	T	A	14: 68,065,951 (GRCm39)	I351F	probably benign	Het
Elmod2	A	C	8: 84,046,041 (GRCm39)		probably null	Het
Endov	G	A	11: 119,398,077 (GRCm39)	A281T	probably benign	Het
Epyc	A	G	10: 97,485,562 (GRCm39)	M1V	probably null	Het
Espnl	T	A	1: 91,251,290 (GRCm39)	H128Q	probably damaging	Het
Fam20a	A	T	11: 109,565,454 (GRCm39)	C452*	probably null	Het
Fam241b	A	G	10: 61,944,733 (GRCm39)	V88A	probably damaging	Het
Fbxl9	A	G	8: 106,042,299 (GRCm39)		probably benign	Het
Fgd3	A	T	13: 49,450,166 (GRCm39)	S28T	possibly damaging	Het
Fmn1	C	A	2: 113,356,025 (GRCm39)	P920Q	unknown	Het
Gpr139	G	C	7: 118,744,089 (GRCm39)	D165E	probably benign	Het
Hoxb1	A	G	11: 96,257,927 (GRCm39)	T286A	possibly damaging	Het
Ifi204	T	A	1: 173,587,134 (GRCm39)	E174D	probably benign	Het
Igf2bp2	A	G	16: 21,880,632 (GRCm39)	I555T	possibly damaging	Het
Ighv1-85	A	G	12: 115,963,799 (GRCm39)	I67T	probably damaging	Het
Ihh	G	C	1: 74,990,306 (GRCm39)	P23R	unknown	Het
Irx2	T	C	13: 72,779,396 (GRCm39)	S227P	probably damaging	Het
Itga4	A	G	2: 79,086,526 (GRCm39)	I68V	probably benign	Het
Jsrp1	T	A	10: 80,647,906 (GRCm39)	T51S	probably benign	Het
Khdc4	T	C	3: 88,619,002 (GRCm39)	S569P	probably damaging	Het
Kifc2	G	T	15: 76,547,010 (GRCm39)	G306V	probably damaging	Het
L3mbtl2	T	A	15: 81,551,588 (GRCm39)	S85T	possibly damaging	Het
Lama1	T	C	17: 68,074,380 (GRCm39)	S944P		Het
Lama4	T	A	10: 38,902,631 (GRCm39)	C202S	probably damaging	Het
Morc3	G	A	16: 93,646,061 (GRCm39)	D218N	probably damaging	Het
Mrtfb	T	A	16: 13,223,718 (GRCm39)	Y866*	probably null	Het
Myorg	A	G	4: 41,498,577 (GRCm39)	I351T	probably damaging	Het
N4bp2l1	G	A	5: 150,496,389 (GRCm39)	T179I	probably damaging	Het
Narf	A	G	11: 121,139,976 (GRCm39)	E270G	probably benign	Het
Nsd2	A	G	5: 34,049,380 (GRCm39)	D1205G	probably damaging	Het
Or10x1	T	C	1: 174,196,759 (GRCm39)	V92A	probably benign	Het
Or5an11	T	C	19: 12,246,068 (GRCm39)	V158A	probably benign	Het
Parl	T	C	16: 20,106,625 (GRCm39)	T195A	probably benign	Het
Phrf1	A	G	7: 140,820,846 (GRCm39)	I158V	unknown	Het
Phtf1	T	C	3: 103,904,980 (GRCm39)	F543L	possibly damaging	Het
Plod2	T	A	9: 92,466,611 (GRCm39)	D190E	probably damaging	Het
Polr1a	G	T	6: 71,927,863 (GRCm39)	K871N	possibly damaging	Het
Ppp2r5a	A	T	1: 191,089,998 (GRCm39)	I252K	probably damaging	Het
Ptgir	T	A	7: 16,640,973 (GRCm39)	D88E	probably damaging	Het
Raly	T	C	2: 154,699,340 (GRCm39)	V48A	probably damaging	Het
Ripor1	T	A	8: 106,344,447 (GRCm39)	L527*	probably null	Het
Rnf213	T	C	11: 119,307,373 (GRCm39)	S678P		Het
Rpf1	T	A	3: 146,212,918 (GRCm39)	Q306L	probably benign	Het
Rpl14	T	C	9: 120,403,171 (GRCm39)	I122T	probably damaging	Het
Scn9a	C	A	2: 66,314,748 (GRCm39)	A1657S	possibly damaging	Het
Serpinb10	A	G	1: 107,474,477 (GRCm39)	Y213C	probably damaging	Het
Slc25a23	A	G	17: 57,359,827 (GRCm39)	L308P	probably damaging	Het
Smg9	T	A	7: 24,120,058 (GRCm39)	M387K	probably benign	Het
Socs3	G	A	11: 117,858,614 (GRCm39)	P148L	probably benign	Het
Susd3	A	G	13: 49,401,906 (GRCm39)	L14P	probably benign	Het
Syt14	T	A	1: 192,662,858 (GRCm39)	M80L	probably benign	Het
Tmprss2	T	A	16: 97,369,616 (GRCm39)	Y386F	possibly damaging	Het
Tmx3	T	A	18: 90,558,195 (GRCm39)	S416T	probably benign	Het
Ttc5	T	G	14: 51,003,400 (GRCm39)	Q428P	probably damaging	Het
Vmn2r73	A	G	7: 85,521,192 (GRCm39)	S259P	possibly damaging	Het
Vps4b	A	T	1: 106,719,434 (GRCm39)	V38E	probably damaging	Het
Wdr83	C	A	8: 85,802,890 (GRCm39)	R177L	probably benign	Het
Zfp780b	A	G	7: 27,662,588 (GRCm39)	F656L	possibly damaging	Het
Zfp995	A	T	17: 22,099,641 (GRCm39)	F198I	probably benign	Het
Zswim8	A	T	14: 20,771,552 (GRCm39)	Y1495F	probably damaging	Het

Other mutations in Treh

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00493:Treh	APN	9	44,595,197 (GRCm39)	missense	probably damaging	1.00
IGL00984:Treh	APN	9	44,594,264 (GRCm39)	unclassified	probably benign
IGL02111:Treh	APN	9	44,594,258 (GRCm39)	missense	probably benign	0.02
IGL02493:Treh	APN	9	44,594,246 (GRCm39)	missense	possibly damaging	0.92
IGL03242:Treh	APN	9	44,596,634 (GRCm39)	missense	possibly damaging	0.51
IGL03392:Treh	APN	9	44,597,228 (GRCm39)	missense	probably damaging	0.96
delight	UTSW	9	44,592,823 (GRCm39)	missense	probably damaging	1.00
trixie	UTSW	9	44,594,910 (GRCm39)	missense	probably benign	0.27
R0041:Treh	UTSW	9	44,594,910 (GRCm39)	missense	probably benign	0.27
R1391:Treh	UTSW	9	44,596,602 (GRCm39)	missense	probably benign	0.02
R2099:Treh	UTSW	9	44,595,943 (GRCm39)	missense	probably damaging	1.00
R2142:Treh	UTSW	9	44,592,438 (GRCm39)	missense	probably damaging	1.00
R3936:Treh	UTSW	9	44,595,840 (GRCm39)	missense	probably benign	0.02
R4577:Treh	UTSW	9	44,597,208 (GRCm39)	missense	probably benign	0.11
R4715:Treh	UTSW	9	44,594,615 (GRCm39)	missense	probably benign	0.44
R4735:Treh	UTSW	9	44,592,849 (GRCm39)	missense	probably damaging	1.00
R4964:Treh	UTSW	9	44,593,945 (GRCm39)	missense	probably damaging	0.99
R5028:Treh	UTSW	9	44,594,186 (GRCm39)	missense	probably null	0.96
R5214:Treh	UTSW	9	44,594,173 (GRCm39)	missense	probably damaging	1.00
R5645:Treh	UTSW	9	44,593,975 (GRCm39)	missense	probably damaging	0.99
R7892:Treh	UTSW	9	44,596,015 (GRCm39)	missense	probably damaging	1.00
R7944:Treh	UTSW	9	44,592,584 (GRCm39)	missense	probably damaging	1.00
R7945:Treh	UTSW	9	44,592,584 (GRCm39)	missense	probably damaging	1.00
R8338:Treh	UTSW	9	44,595,808 (GRCm39)	missense	probably benign	0.27
R8818:Treh	UTSW	9	44,592,823 (GRCm39)	missense	probably damaging	1.00
R8884:Treh	UTSW	9	44,595,800 (GRCm39)	unclassified	probably benign
R9041:Treh	UTSW	9	44,596,677 (GRCm39)	missense	probably damaging	1.00
R9311:Treh	UTSW	9	44,592,655 (GRCm39)	missense	probably benign	0.39
R9489:Treh	UTSW	9	44,592,416 (GRCm39)	missense	probably damaging	1.00
R9605:Treh	UTSW	9	44,592,416 (GRCm39)	missense	probably damaging	1.00
R9701:Treh	UTSW	9	44,594,648 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GAGTCAGAATGGCAATCATTGG -3'
(R):5'- TGGTCACTTCTTGGCTCAGG -3'

Sequencing Primer
(F):5'- AATGGCAATCATTGGTGCCC -3'
(R):5'- ACTTCTTGGCTCAGGACACTATGAG -3'

Posted On

2019-06-26