Mutagenetix > Incidental Mutation

Incidental Mutation 'R7313:Ptgfrn'

567717

Institutional Source

Beutler Lab

Gene Symbol

Ptgfrn

Ensembl Gene

ENSMUSG00000027864

Gene Name

prostaglandin F2 receptor negative regulator

Synonyms

CD9P-1, 4833445A08Rik

MMRRC Submission

045411-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7313 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

101040232-101110278 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 101073047 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Phenylalanine at position 326 (L326F)

Ref Sequence

ENSEMBL: ENSMUSP00000099755 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000102694]

AlphaFold

Q9WV91

Predicted Effect

possibly damaging
Transcript: ENSMUST00000102694
AA Change: L326F

PolyPhen 2 Score 0.839 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000099755
Gene: ENSMUSG00000027864
AA Change: L326F

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
IGv	38	121	3.01e-7	SMART
IG	154	264	1.54e-4	SMART
IG	284	390	1.11e-5	SMART
IG	414	532	1.72e-2	SMART
IG	556	676	9.71e-2	SMART
IG	696	822	5.21e-2	SMART
transmembrane domain	831	853	N/A	INTRINSIC
low complexity region	862	872	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 97.8%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Homozygous mice for a null gene trap mutation exhibit a decreased depressive-like response during tail suspension testing when compared with their wild-type littermates, [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 110 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb1a	A	G	5: 8,723,187 (GRCm38)	N805S	probably damaging	Het
Acat2	A	G	17: 12,960,006 (GRCm38)	V60A	probably benign	Het
Adam6b	A	G	12: 113,491,134 (GRCm38)	I524V	probably benign	Het
Adamts16	C	T	13: 70,772,955 (GRCm38)	W590*	probably null	Het
Adamts9	C	T	6: 92,858,121 (GRCm38)	G1257D	probably damaging	Het
Adgrf5	C	T	17: 43,445,083 (GRCm38)	T644I	probably benign	Het
Adgrf5	T	G	17: 43,452,477 (GRCm38)		probably null	Het
Adgrv1	T	C	13: 81,520,515 (GRCm38)	T2641A	possibly damaging	Het
Agap3	T	C	5: 24,452,384 (GRCm38)	F60L	probably benign	Het
Akap9	C	A	5: 4,004,933 (GRCm38)	T1626K	probably damaging	Het
Ampd3	C	A	7: 110,806,054 (GRCm38)	D603E	probably damaging	Het
Angpt4	T	A	2: 151,925,406 (GRCm38)	V119E	probably benign	Het
Atp6v1a	T	C	16: 44,114,617 (GRCm38)	T70A	probably benign	Het
B4galt3	T	A	1: 171,272,749 (GRCm38)	I163N	probably damaging	Het
Borcs5	C	T	6: 134,710,180 (GRCm38)	T167M	probably damaging	Het
Btbd19	T	A	4: 117,121,419 (GRCm38)	S156C	probably damaging	Het
Camkk2	C	T	5: 122,737,511 (GRCm38)	R492Q	possibly damaging	Het
Casr	T	C	16: 36,509,671 (GRCm38)	I434V	probably damaging	Het
Cd4	G	T	6: 124,867,103 (GRCm38)	T394K	probably benign	Het
Cps1	T	C	1: 67,198,358 (GRCm38)	L1006P	probably damaging	Het
Crx	G	A	7: 15,867,932 (GRCm38)	P274S	probably damaging	Het
Crybg1	T	A	10: 43,989,111 (GRCm38)	I1457F	probably damaging	Het
Cul4a	C	A	8: 13,121,676 (GRCm38)		probably benign	Het
D430041D05Rik	T	A	2: 104,255,565 (GRCm38)	T196S	probably benign	Het
Dnah3	T	C	7: 119,981,344 (GRCm38)	E1995G	probably benign	Het
Dnm1	T	A	2: 32,336,009 (GRCm38)	T353S	probably damaging	Het
E430018J23Rik	C	T	7: 127,391,684 (GRCm38)	S377N	probably benign	Het
Ect2l	T	A	10: 18,168,401 (GRCm38)	T329S	probably damaging	Het
Elp2	T	A	18: 24,609,659 (GRCm38)	S83T	probably benign	Het
Exosc7	A	G	9: 123,118,948 (GRCm38)	T39A	probably benign	Het
Fam135a	C	A	1: 24,057,392 (GRCm38)	V91F	probably damaging	Het
Gab2	G	T	7: 97,081,798 (GRCm38)		probably benign	Het
Gbf1	A	T	19: 46,280,354 (GRCm38)	I1408F	possibly damaging	Het
Glis3	C	T	19: 28,531,019 (GRCm38)	E522K	probably damaging	Het
Gm10375	A	T	14: 43,604,857 (GRCm38)	C139S	possibly damaging	Het
Gm26558	T	C	2: 70,661,867 (GRCm38)	E81G	unknown	Het
Gm35315	C	T	5: 110,079,225 (GRCm38)	C116Y	probably benign	Het
Gm3676	T	A	14: 41,644,107 (GRCm38)	I84F	probably damaging	Het
Gm7145	T	A	1: 117,986,202 (GRCm38)	H271Q	probably damaging	Het
Gpat2	T	A	2: 127,428,295 (GRCm38)	I76N	probably damaging	Het
Hipk1	A	T	3: 103,778,258 (GRCm38)	S14T	unknown	Het
Hlcs	C	A	16: 94,267,503 (GRCm38)	S286I	probably damaging	Het
Ighg1	A	T	12: 113,329,458 (GRCm38)	F204Y		Het
Igsf10	T	C	3: 59,329,416 (GRCm38)	I1115V	probably benign	Het
Klk7	A	T	7: 43,812,875 (GRCm38)	H97L	probably damaging	Het
Kmt2d	A	T	15: 98,856,623 (GRCm38)	D1605E	unknown	Het
Leng8	T	A	7: 4,139,526 (GRCm38)	I49N	possibly damaging	Het
Lingo4	A	T	3: 94,403,144 (GRCm38)	D463V	possibly damaging	Het
Lrp1	T	A	10: 127,553,468 (GRCm38)	N3193I	probably damaging	Het
Lrrc7	T	A	3: 158,160,474 (GRCm38)	Y1210F	probably damaging	Het
Mki67	G	C	7: 135,694,671 (GRCm38)	A2878G	probably benign	Het
Mmp1a	TG	TGG	9: 7,465,083 (GRCm38)		probably null	Het
Mucl2	C	T	15: 103,899,179 (GRCm38)		probably null	Het
Myh6	A	T	14: 54,960,270 (GRCm38)	D470E	probably benign	Het
Myo1e	T	C	9: 70,359,385 (GRCm38)		probably null	Het
Myo7a	G	A	7: 98,064,195 (GRCm38)	R1647W	probably damaging	Het
Nbeal1	G	C	1: 60,237,151 (GRCm38)	V684L	probably benign	Het
Nmral1	C	T	16: 4,713,796 (GRCm38)	M198I	probably benign	Het
Nrap	C	A	19: 56,342,268 (GRCm38)	L1120F	probably damaging	Het
Nup155	T	A	15: 8,154,922 (GRCm38)	I1267N	probably damaging	Het
Obscn	G	A	11: 59,007,588 (GRCm38)	P6616S	unknown	Het
Ocstamp	T	G	2: 165,397,309 (GRCm38)	D319A	probably damaging	Het
Olfr384	A	T	11: 73,602,984 (GRCm38)	I135F	probably damaging	Het
Olfr600	A	G	7: 103,346,331 (GRCm38)	V199A	probably benign	Het
Olfr787	T	C	10: 129,462,987 (GRCm38)	S104P	probably damaging	Het
Olfr796	A	G	10: 129,608,080 (GRCm38)	S134P	probably benign	Het
Olfr855	A	G	9: 19,584,642 (GRCm38)	Y35C	probably damaging	Het
Ooep	T	C	9: 78,378,151 (GRCm38)	D61G	probably damaging	Het
Otoa	A	G	7: 121,102,542 (GRCm38)	E148G	probably benign	Het
Ovgp1	A	T	3: 105,987,071 (GRCm38)	D720V	unknown	Het
Padi2	T	A	4: 140,932,768 (GRCm38)	F294I	probably damaging	Het
Pcdha7	T	A	18: 36,974,418 (GRCm38)	N165K	probably damaging	Het
Pcdhb6	C	A	18: 37,335,208 (GRCm38)	P394H	probably damaging	Het
Pcdhga6	T	C	18: 37,708,019 (GRCm38)	V264A	possibly damaging	Het
Pik3ap1	G	T	19: 41,296,376 (GRCm38)	D623E	possibly damaging	Het
Prdm10	A	T	9: 31,357,160 (GRCm38)	K802*	probably null	Het
Rgsl1	T	G	1: 153,807,876 (GRCm38)		probably null	Het
Rock1	T	G	18: 10,129,317 (GRCm38)	T347P	possibly damaging	Het
Rprd2	G	A	3: 95,776,710 (GRCm38)	P338S	probably damaging	Het
Sdk1	A	T	5: 141,937,622 (GRCm38)	N333Y	probably damaging	Het
Setd4	T	A	16: 93,591,244 (GRCm38)	H118L	probably benign	Het
Sirt3	G	A	7: 140,878,126 (GRCm38)	P37S		Het
Slc16a9	G	T	10: 70,283,170 (GRCm38)	G440W	probably damaging	Het
Slc22a23	T	A	13: 34,183,178 (GRCm38)	I616F	probably damaging	Het
Slc30a8	A	C	15: 52,317,311 (GRCm38)	D102A	probably damaging	Het
Slc6a4	T	A	11: 77,010,701 (GRCm38)	D87E	possibly damaging	Het
Slc9a4	A	C	1: 40,629,503 (GRCm38)	T769P	probably benign	Het
Soga3	T	A	10: 29,196,879 (GRCm38)	Y722*	probably null	Het
Sspo	C	A	6: 48,473,456 (GRCm38)	Q2560K	probably benign	Het
Sspo	A	T	6: 48,454,828 (GRCm38)	Y685F	probably damaging	Het
Stpg1	T	A	4: 135,529,516 (GRCm38)	L206Q	probably damaging	Het
Stt3b	A	T	9: 115,266,115 (GRCm38)	Y283N	probably damaging	Het
Sult4a1	T	C	15: 84,086,613 (GRCm38)	E197G	probably damaging	Het
Syne1	T	G	10: 5,047,635 (GRCm38)	D444A	probably damaging	Het
Tex15	T	A	8: 33,574,817 (GRCm38)	V1425E	possibly damaging	Het
Tgm4	A	G	9: 123,062,491 (GRCm38)	D557G	probably benign	Het
Tmcc3	A	G	10: 94,430,572 (GRCm38)		probably benign	Het
Tnfrsf8	T	A	4: 145,274,382 (GRCm38)	N385Y	probably benign	Het
Ttc4	T	C	4: 106,678,820 (GRCm38)	D15G	possibly damaging	Het
Tut1	A	G	19: 8,964,049 (GRCm38)	N400S	probably benign	Het
Usp39	T	C	6: 72,336,430 (GRCm38)	K259R	probably benign	Het
Vmn1r215	C	T	13: 23,076,314 (GRCm38)	H175Y	probably benign	Het
Vmn1r36	A	T	6: 66,716,123 (GRCm38)	M256K	probably benign	Het
Zfp128	T	A	7: 12,890,534 (GRCm38)	H276Q	possibly damaging	Het
Zfp276	T	C	8: 123,267,823 (GRCm38)	M543T	probably damaging	Het
Zfp354c	A	T	11: 50,814,656 (GRCm38)	Y531N	probably damaging	Het
Zfp532	T	A	18: 65,623,005 (GRCm38)	M3K	probably damaging	Het
Zfp64	T	G	2: 168,899,890 (GRCm38)	K373Q	probably damaging	Het
Zfp983	T	G	17: 21,661,497 (GRCm38)	S114A	probably damaging	Het
Zranb1	G	A	7: 132,982,752 (GRCm38)	R583K	probably damaging	Het

Other mutations in Ptgfrn

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00949:Ptgfrn	APN	3	101,072,845 (GRCm38)	missense	probably benign	0.01
IGL01710:Ptgfrn	APN	3	101,073,088 (GRCm38)	missense	probably damaging	0.98
IGL02557:Ptgfrn	APN	3	101,060,636 (GRCm38)	critical splice donor site	probably null
IGL02740:Ptgfrn	APN	3	101,072,937 (GRCm38)	missense	possibly damaging	0.84
IGL02817:Ptgfrn	APN	3	101,060,752 (GRCm38)	missense	probably benign
IGL02948:Ptgfrn	APN	3	101,072,819 (GRCm38)	missense	probably benign	0.21
R1540:Ptgfrn	UTSW	3	101,060,654 (GRCm38)	missense	probably benign	0.41
R1563:Ptgfrn	UTSW	3	101,060,651 (GRCm38)	missense	possibly damaging	0.67
R1730:Ptgfrn	UTSW	3	101,056,442 (GRCm38)	missense	possibly damaging	0.71
R1766:Ptgfrn	UTSW	3	101,050,122 (GRCm38)	missense	probably benign	0.00
R1783:Ptgfrn	UTSW	3	101,056,442 (GRCm38)	missense	possibly damaging	0.71
R1918:Ptgfrn	UTSW	3	101,056,307 (GRCm38)	missense	probably benign
R2113:Ptgfrn	UTSW	3	101,077,309 (GRCm38)	missense	probably benign	0.00
R2290:Ptgfrn	UTSW	3	101,077,361 (GRCm38)	missense	possibly damaging	0.77
R3522:Ptgfrn	UTSW	3	101,043,402 (GRCm38)	missense	probably damaging	1.00
R5223:Ptgfrn	UTSW	3	101,045,593 (GRCm38)	missense	probably benign	0.13
R5600:Ptgfrn	UTSW	3	101,056,250 (GRCm38)	missense	probably damaging	0.99
R5642:Ptgfrn	UTSW	3	101,043,362 (GRCm38)	missense	probably damaging	1.00
R5927:Ptgfrn	UTSW	3	101,060,652 (GRCm38)	missense	possibly damaging	0.92
R5984:Ptgfrn	UTSW	3	101,050,143 (GRCm38)	missense	probably damaging	0.99
R6124:Ptgfrn	UTSW	3	101,073,089 (GRCm38)	missense	probably damaging	0.98
R6331:Ptgfrn	UTSW	3	101,045,620 (GRCm38)	missense	possibly damaging	0.64
R6363:Ptgfrn	UTSW	3	101,045,578 (GRCm38)	missense	possibly damaging	0.93
R6473:Ptgfrn	UTSW	3	101,045,639 (GRCm38)	missense	probably damaging	1.00
R6856:Ptgfrn	UTSW	3	101,045,446 (GRCm38)	missense	probably damaging	1.00
R7151:Ptgfrn	UTSW	3	101,080,195 (GRCm38)	nonsense	probably null
R7361:Ptgfrn	UTSW	3	101,077,444 (GRCm38)	missense	probably benign	0.03
R7806:Ptgfrn	UTSW	3	101,077,132 (GRCm38)	missense	possibly damaging	0.50
R7823:Ptgfrn	UTSW	3	101,043,409 (GRCm38)	missense	probably damaging	1.00
R7841:Ptgfrn	UTSW	3	101,060,810 (GRCm38)	missense	probably damaging	0.98
R8093:Ptgfrn	UTSW	3	101,072,941 (GRCm38)	missense	probably benign	0.09
R8093:Ptgfrn	UTSW	3	101,056,437 (GRCm38)	missense	probably benign	0.19
R8490:Ptgfrn	UTSW	3	101,056,370 (GRCm38)	missense	probably damaging	0.99
R8856:Ptgfrn	UTSW	3	101,056,611 (GRCm38)	missense	possibly damaging	0.86
Z1088:Ptgfrn	UTSW	3	101,056,437 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGGTCACGCTTACACCAGAC -3'
(R):5'- AATCAACCTAAAGGGGCGAC -3'

Sequencing Primer
(F):5'- TTACACCAGACGGGGCAGAC -3'
(R):5'- ACGCGTCACTCGATCACTCG -3'

Posted On

2019-06-26