Mutagenetix > Incidental Mutation

Incidental Mutation 'R7314:Lingo3'

567833

Institutional Source

Beutler Lab

Gene Symbol

Lingo3

Ensembl Gene

ENSMUSG00000051067

Gene Name

leucine rich repeat and Ig domain containing 3

Synonyms

LERN2

MMRRC Submission

045412-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R7314 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

80668635-80679873 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 80670707 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 408 (I408V)

Ref Sequence

ENSEMBL: ENSMUSP00000054960 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053986] [ENSMUST00000219924]

AlphaFold

Q6GQU6

Predicted Effect

possibly damaging
Transcript: ENSMUST00000053986
AA Change: I408V

PolyPhen 2 Score 0.924 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000054960
Gene: ENSMUSG00000051067
AA Change: I408V

Domain	Start	End	E-Value	Type
LRRNT	23	57	1.54e-5	SMART
LRR_TYP	76	99	1.38e-3	SMART
LRR_TYP	100	123	4.94e-5	SMART
LRR	124	147	3.86e0	SMART
LRR	148	171	4.98e-1	SMART
LRR	172	195	1.62e1	SMART
LRR	246	267	3.46e2	SMART
LRR	269	291	3.86e0	SMART
LRR	292	315	3.24e0	SMART
LRR	316	339	4.34e-1	SMART
LRRCT	351	404	7.18e-3	SMART
IGc2	419	486	3.12e-14	SMART
transmembrane domain	526	548	N/A	INTRINSIC
low complexity region	571	581	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000219924

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (44/44)

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700020L13Rik	G	A	7: 29,989,320 (GRCm39)	V177I	unknown	Het
4930596D02Rik	A	G	14: 35,533,606 (GRCm39)	V54A	probably benign	Het
Abcc10	C	A	17: 46,626,330 (GRCm39)	A704S	probably damaging	Het
Adss2	A	G	1: 177,595,317 (GRCm39)	W408R	probably damaging	Het
Aplp1	T	C	7: 30,135,414 (GRCm39)	E548G	probably damaging	Het
C4b	C	T	17: 34,959,330 (GRCm39)	V415I	probably benign	Het
Celsr3	T	C	9: 108,706,343 (GRCm39)	V942A	probably damaging	Het
Cstf1	A	G	2: 172,214,954 (GRCm39)	D25G	probably damaging	Het
Dnah14	A	T	1: 181,612,819 (GRCm39)		probably null	Het
Dnah9	G	A	11: 65,880,677 (GRCm39)	T2640I	probably benign	Het
Dnase2b	T	C	3: 146,288,151 (GRCm39)	I315V	probably damaging	Het
Endod1	T	C	9: 14,268,295 (GRCm39)	S397G	probably benign	Het
Eps8	A	G	6: 137,504,090 (GRCm39)	V171A	possibly damaging	Het
Ghdc	T	C	11: 100,659,928 (GRCm39)	E273G	probably damaging	Het
Hmgn2-ps	C	T	8: 73,058,839 (GRCm39)	G63R	probably damaging	Het
Hspbap1	T	A	16: 35,645,541 (GRCm39)	S409T	probably benign	Het
Ireb2	T	C	9: 54,799,794 (GRCm39)	Y412H	probably damaging	Het
Jph4	TCCATTCTCGTATACCCCA	TCCA	14: 55,347,196 (GRCm39)		probably benign	Het
Klhl6	A	G	16: 19,775,755 (GRCm39)	Y268H	probably damaging	Het
Krt16	T	C	11: 100,138,695 (GRCm39)	D197G	probably damaging	Het
Lca5	T	C	9: 83,277,563 (GRCm39)	K594E	possibly damaging	Het
Lgi3	T	C	14: 70,769,552 (GRCm39)	F84S	probably damaging	Het
Map3k7	G	T	4: 31,985,769 (GRCm39)	E231*	probably null	Het
Nrip1	A	G	16: 76,088,078 (GRCm39)	S1160P	probably benign	Het
Oas1g	A	T	5: 121,016,526 (GRCm39)	L301Q	probably damaging	Het
Obox3	T	A	7: 15,361,079 (GRCm39)	Q62L	possibly damaging	Het
Or5d37	A	G	2: 87,923,458 (GRCm39)	L274P	probably benign	Het
Or6b13	A	T	7: 139,782,326 (GRCm39)	V119D	probably damaging	Het
Parp8	G	T	13: 117,004,996 (GRCm39)	F727L	probably benign	Het
Pcdha1	T	C	18: 37,064,553 (GRCm39)	Y406H	probably damaging	Het
Pcdhgb8	A	G	18: 37,896,052 (GRCm39)	D374G	probably damaging	Het
Pdzd8	A	T	19: 59,289,783 (GRCm39)	L539*	probably null	Het
Phkb	A	G	8: 86,669,021 (GRCm39)		probably null	Het
Ppp1r13b	T	C	12: 111,812,790 (GRCm39)	E143G	probably damaging	Het
Rpap2	T	C	5: 107,768,245 (GRCm39)	V361A	probably damaging	Het
Setd4	T	C	16: 93,384,711 (GRCm39)	T326A	probably benign	Het
Smad9	A	G	3: 54,696,744 (GRCm39)	N270D	probably benign	Het
Sntg2	A	G	12: 30,317,107 (GRCm39)	S172P	probably benign	Het
Tecpr1	A	G	5: 144,154,150 (GRCm39)	L101P	probably damaging	Het
Tmco3	G	A	8: 13,369,605 (GRCm39)		probably null	Het
Tmprss11f	T	C	5: 86,671,912 (GRCm39)	T427A	possibly damaging	Het
Trhr2	A	G	8: 123,085,489 (GRCm39)	V165A	possibly damaging	Het
Ubr3	T	A	2: 69,821,944 (GRCm39)	L1402Q	probably damaging	Het
Vps13c	T	A	9: 67,850,622 (GRCm39)		probably null	Het
Zfp369	T	C	13: 65,439,918 (GRCm39)	S201P	probably damaging	Het

Other mutations in Lingo3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01141:Lingo3	APN	10	80,671,147 (GRCm39)	missense	probably damaging	1.00
IGL02120:Lingo3	APN	10	80,671,693 (GRCm39)	missense	probably damaging	1.00
IGL02755:Lingo3	APN	10	80,671,843 (GRCm39)	missense	possibly damaging	0.49
IGL02938:Lingo3	APN	10	80,670,988 (GRCm39)	missense	probably benign	0.00
IGL02945:Lingo3	APN	10	80,670,532 (GRCm39)	missense	probably damaging	1.00
IGL03167:Lingo3	APN	10	80,671,178 (GRCm39)	missense	probably damaging	1.00
R0639:Lingo3	UTSW	10	80,671,618 (GRCm39)	missense	probably benign	0.11
R0645:Lingo3	UTSW	10	80,671,169 (GRCm39)	missense	probably benign	0.00
R0673:Lingo3	UTSW	10	80,671,618 (GRCm39)	missense	probably benign	0.11
R1250:Lingo3	UTSW	10	80,670,605 (GRCm39)	missense	probably benign	0.05
R1521:Lingo3	UTSW	10	80,671,555 (GRCm39)	missense	probably benign
R1794:Lingo3	UTSW	10	80,671,432 (GRCm39)	missense	probably benign	0.19
R4665:Lingo3	UTSW	10	80,671,372 (GRCm39)	missense	probably damaging	1.00
R5587:Lingo3	UTSW	10	80,671,364 (GRCm39)	missense	probably damaging	0.98
R6458:Lingo3	UTSW	10	80,671,150 (GRCm39)	missense	probably damaging	1.00
R7082:Lingo3	UTSW	10	80,671,625 (GRCm39)	missense	probably benign
R7231:Lingo3	UTSW	10	80,670,938 (GRCm39)	missense	possibly damaging	0.86
R7390:Lingo3	UTSW	10	80,670,463 (GRCm39)	missense	probably damaging	1.00
R7450:Lingo3	UTSW	10	80,670,671 (GRCm39)	nonsense	probably null
R7650:Lingo3	UTSW	10	80,671,597 (GRCm39)	missense	probably damaging	1.00
R7894:Lingo3	UTSW	10	80,670,610 (GRCm39)	nonsense	probably null
R7920:Lingo3	UTSW	10	80,670,382 (GRCm39)	missense	probably benign	0.09
R8070:Lingo3	UTSW	10	80,671,955 (GRCm39)	start gained	probably benign
R8095:Lingo3	UTSW	10	80,671,255 (GRCm39)	missense	probably benign	0.01
R8171:Lingo3	UTSW	10	80,670,595 (GRCm39)	missense	probably benign
R8178:Lingo3	UTSW	10	80,670,464 (GRCm39)	missense	possibly damaging	0.80
R8425:Lingo3	UTSW	10	80,670,816 (GRCm39)	missense	probably benign	0.04
R9053:Lingo3	UTSW	10	80,670,821 (GRCm39)	missense	probably benign	0.00
R9059:Lingo3	UTSW	10	80,670,523 (GRCm39)	missense	probably benign	0.12
R9706:Lingo3	UTSW	10	80,670,288 (GRCm39)	missense	probably damaging	0.99
R9794:Lingo3	UTSW	10	80,670,707 (GRCm39)	missense	possibly damaging	0.92
Z1176:Lingo3	UTSW	10	80,670,689 (GRCm39)	missense	possibly damaging	0.95

Predicted Primers

PCR Primer
(F):5'- CACATGTGTAGGTGCCACTGTC -3'
(R):5'- CATTCCACTCGGTGAACACG -3'

Sequencing Primer
(F):5'- TAGGTGCCACTGTCCTGGG -3'
(R):5'- TCGGTGAACACGCTCGAGAC -3'

Posted On

2019-06-26