Mutagenetix > Incidental Mutation

Incidental Mutation 'R0638:Lrrc66'

56806

Institutional Source

Beutler Lab

Gene Symbol

Lrrc66

Ensembl Gene

ENSMUSG00000067206

Gene Name

leucine rich repeat containing 66

Synonyms

MMRRC Submission

038827-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.056) question?

Stock #

R0638 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

73763985-73789771 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

T to A at 73772816 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000084423 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000087177]

AlphaFold

Q8K0B3

Predicted Effect

probably benign
Transcript: ENSMUST00000087177

SMART Domains

Protein: ENSMUSP00000084423
Gene: ENSMUSG00000067206

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
LRR	140	162	1.81e1	SMART
LRR_TYP	163	186	9.44e-2	SMART
LRR	187	210	1.26e2	SMART
LRR	211	234	4.84e1	SMART
low complexity region	298	309	N/A	INTRINSIC
transmembrane domain	369	391	N/A	INTRINSIC
low complexity region	434	445	N/A	INTRINSIC
low complexity region	842	859	N/A	INTRINSIC

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.1%
20x: 93.5%

Validation Efficiency

99% (76/77)

Allele List at MGI

All alleles(2) : Targeted, other(1) Gene trapped(1)

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4932414N04Rik	C	A	2: 68,547,572 (GRCm39)	Q161K	probably benign	Het
Aatk	A	T	11: 119,900,748 (GRCm39)	L1216Q	probably damaging	Het
Aifm3	T	C	16: 17,321,535 (GRCm39)	F463L	possibly damaging	Het
Antxr2	C	T	5: 98,108,496 (GRCm39)	W338*	probably null	Het
Apc2	T	C	10: 80,140,801 (GRCm39)	S219P	probably damaging	Het
Arfgap3	A	T	15: 83,192,389 (GRCm39)		probably null	Het
Arrdc5	A	G	17: 56,607,020 (GRCm39)	V75A	possibly damaging	Het
Atg16l2	A	T	7: 100,949,317 (GRCm39)		probably null	Het
Cacna1i	A	G	15: 80,265,281 (GRCm39)	N1511S	possibly damaging	Het
Cad	T	C	5: 31,235,032 (GRCm39)	Y2095H	probably damaging	Het
Chia1	T	C	3: 106,035,753 (GRCm39)		probably benign	Het
Crybg2	A	G	4: 133,801,765 (GRCm39)	D975G	probably damaging	Het
Dagla	T	C	19: 10,232,247 (GRCm39)	I480V	probably damaging	Het
Efl1	C	T	7: 82,301,095 (GRCm39)	T33I	probably damaging	Het
Esp36	A	G	17: 38,728,060 (GRCm39)	F74L	probably benign	Het
Faim	T	C	9: 98,874,149 (GRCm39)		probably benign	Het
Fam83h	G	T	15: 75,875,776 (GRCm39)	H520Q	probably benign	Het
Fbn2	A	T	18: 58,178,446 (GRCm39)	C1931S	probably damaging	Het
Frs3	A	G	17: 48,012,581 (GRCm39)	D96G	probably benign	Het
Gbp4	A	G	5: 105,269,706 (GRCm39)	M374T	probably damaging	Het
Gimap1	C	T	6: 48,718,359 (GRCm39)		probably benign	Het
Gm10010	A	G	6: 128,177,576 (GRCm39)		noncoding transcript	Het
Gm10355	T	C	3: 101,214,214 (GRCm39)		noncoding transcript	Het
Gmip	C	T	8: 70,264,095 (GRCm39)		probably benign	Het
Gpc2	A	T	5: 138,276,796 (GRCm39)	F110Y	possibly damaging	Het
Ifi44l	C	T	3: 151,468,396 (GRCm39)	V45M	probably benign	Het
Il15	T	C	8: 83,069,890 (GRCm39)	E58G	probably damaging	Het
Kat2b	T	C	17: 53,951,771 (GRCm39)		probably benign	Het
Kcnh7	C	A	2: 62,607,854 (GRCm39)	V576L	probably benign	Het
Mical1	A	G	10: 41,358,235 (GRCm39)	E416G	probably benign	Het
Mroh3	A	G	1: 136,118,740 (GRCm39)	Y526H	probably damaging	Het
Mtx2	T	C	2: 74,699,634 (GRCm39)		probably benign	Het
Naip6	A	T	13: 100,437,036 (GRCm39)	Y496N	probably benign	Het
Nfyc	A	G	4: 120,626,081 (GRCm39)	S73P	probably benign	Het
Or10v9	C	T	19: 11,832,487 (GRCm39)	V277M	probably damaging	Het
Or10v9	A	C	19: 11,832,732 (GRCm39)	V195G	probably damaging	Het
Or1e23	T	A	11: 73,407,750 (GRCm39)	I92F	probably damaging	Het
Or5ak25	A	G	2: 85,268,845 (GRCm39)	I219T	probably benign	Het
Or6c69b	T	A	10: 129,627,101 (GRCm39)	D119V	probably damaging	Het
P2ry14	A	G	3: 59,022,869 (GRCm39)	V206A	probably benign	Het
Polg	G	A	7: 79,109,896 (GRCm39)		probably benign	Het
Potefam1	T	C	2: 111,030,763 (GRCm39)	E382G	probably damaging	Het
Ptgs1	G	A	2: 36,130,868 (GRCm39)		probably benign	Het
Pus7l	A	G	15: 94,421,298 (GRCm39)	S671P	probably benign	Het
Ralgapa2	T	C	2: 146,184,112 (GRCm39)	T1547A	probably benign	Het
Rif1	T	C	2: 52,001,600 (GRCm39)	S1685P	probably benign	Het
Rnf213	T	C	11: 119,361,036 (GRCm39)	Y4452H	probably damaging	Het
Samd7	A	G	3: 30,810,670 (GRCm39)	D229G	probably benign	Het
Serpina3j	T	C	12: 104,281,078 (GRCm39)	S84P	possibly damaging	Het
Slc35d1	A	G	4: 103,070,441 (GRCm39)		probably benign	Het
Sorbs2	A	G	8: 46,249,347 (GRCm39)	D847G	probably damaging	Het
Sp110	A	C	1: 85,505,050 (GRCm39)	F434C	probably benign	Het
Steap4	T	C	5: 8,027,030 (GRCm39)		probably benign	Het
Tg	A	C	15: 66,589,057 (GRCm39)	T13P	probably damaging	Het
Timeless	T	A	10: 128,080,542 (GRCm39)	Y474*	probably null	Het
Tmem94	T	C	11: 115,682,886 (GRCm39)		probably null	Het
Trdmt1	G	A	2: 13,521,459 (GRCm39)		probably benign	Het
Trim23	T	C	13: 104,337,817 (GRCm39)	Y522H	probably benign	Het
Ttc13	A	T	8: 125,401,105 (GRCm39)	S624T	probably damaging	Het
Txnl1	A	G	18: 63,825,135 (GRCm39)		probably benign	Het
Unkl	T	C	17: 25,427,057 (GRCm39)		probably benign	Het
Usp54	T	A	14: 20,639,437 (GRCm39)		probably benign	Het
Vcam1	T	C	3: 115,910,908 (GRCm39)	K497E	possibly damaging	Het
Vmn1r49	C	A	6: 90,049,648 (GRCm39)	S118I	possibly damaging	Het
Vmn2r118	T	C	17: 55,915,466 (GRCm39)	K495E	probably benign	Het
Wrnip1	G	A	13: 33,005,073 (GRCm39)	C560Y	possibly damaging	Het
Xkr5	T	C	8: 18,983,563 (GRCm39)	R660G	probably benign	Het
Zfp280c	A	G	X: 47,637,580 (GRCm39)		probably benign	Het
Zfp707	G	A	15: 75,846,978 (GRCm39)	A291T	possibly damaging	Het

Other mutations in Lrrc66

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00507:Lrrc66	APN	5	73,764,457 (GRCm39)	missense	probably benign	0.10
IGL00913:Lrrc66	APN	5	73,765,499 (GRCm39)	missense	possibly damaging	0.76
IGL00954:Lrrc66	APN	5	73,765,741 (GRCm39)	missense	possibly damaging	0.94
IGL01061:Lrrc66	APN	5	73,772,842 (GRCm39)	missense	probably benign	0.01
IGL01343:Lrrc66	APN	5	73,765,806 (GRCm39)	missense	probably damaging	0.98
IGL01714:Lrrc66	APN	5	73,787,320 (GRCm39)	missense	probably benign	0.10
IGL01822:Lrrc66	APN	5	73,787,311 (GRCm39)	missense	probably benign	0.17
IGL02005:Lrrc66	APN	5	73,766,077 (GRCm39)	missense	possibly damaging	0.83
IGL02166:Lrrc66	APN	5	73,764,634 (GRCm39)	missense	probably damaging	0.99
IGL02380:Lrrc66	APN	5	73,787,009 (GRCm39)	missense	possibly damaging	0.55
IGL03162:Lrrc66	APN	5	73,764,725 (GRCm39)	missense	probably benign
BB002:Lrrc66	UTSW	5	73,765,835 (GRCm39)	missense	possibly damaging	0.81
BB012:Lrrc66	UTSW	5	73,765,835 (GRCm39)	missense	possibly damaging	0.81
D4043:Lrrc66	UTSW	5	73,764,869 (GRCm39)	missense	probably benign	0.03
R0126:Lrrc66	UTSW	5	73,764,431 (GRCm39)	missense	probably benign	0.10
R0437:Lrrc66	UTSW	5	73,765,030 (GRCm39)	missense	probably benign	0.23
R0658:Lrrc66	UTSW	5	73,768,287 (GRCm39)	missense	probably benign	0.40
R0729:Lrrc66	UTSW	5	73,765,757 (GRCm39)	missense	probably benign
R1603:Lrrc66	UTSW	5	73,764,769 (GRCm39)	missense	possibly damaging	0.61
R1774:Lrrc66	UTSW	5	73,768,198 (GRCm39)	missense	probably benign	0.16
R1831:Lrrc66	UTSW	5	73,764,769 (GRCm39)	missense	possibly damaging	0.61
R1832:Lrrc66	UTSW	5	73,764,769 (GRCm39)	missense	possibly damaging	0.61
R1902:Lrrc66	UTSW	5	73,764,965 (GRCm39)	missense	probably damaging	0.99
R2858:Lrrc66	UTSW	5	73,764,646 (GRCm39)	missense	probably benign	0.08
R4097:Lrrc66	UTSW	5	73,765,047 (GRCm39)	missense	possibly damaging	0.94
R4164:Lrrc66	UTSW	5	73,787,119 (GRCm39)	splice site	probably null
R4582:Lrrc66	UTSW	5	73,765,580 (GRCm39)	missense	possibly damaging	0.94
R4708:Lrrc66	UTSW	5	73,787,005 (GRCm39)	missense	probably benign	0.06
R4856:Lrrc66	UTSW	5	73,765,910 (GRCm39)	missense	probably benign	0.10
R4886:Lrrc66	UTSW	5	73,765,910 (GRCm39)	missense	probably benign	0.10
R5074:Lrrc66	UTSW	5	73,765,354 (GRCm39)	missense	probably damaging	1.00
R5464:Lrrc66	UTSW	5	73,765,622 (GRCm39)	missense	probably benign	0.05
R5640:Lrrc66	UTSW	5	73,765,977 (GRCm39)	missense	probably benign	0.00
R5709:Lrrc66	UTSW	5	73,766,206 (GRCm39)	missense	probably benign
R5811:Lrrc66	UTSW	5	73,772,860 (GRCm39)	missense	possibly damaging	0.82
R6146:Lrrc66	UTSW	5	73,765,432 (GRCm39)	missense	probably benign	0.10
R7037:Lrrc66	UTSW	5	73,764,504 (GRCm39)	missense	probably benign	0.10
R7041:Lrrc66	UTSW	5	73,765,899 (GRCm39)	missense	possibly damaging	0.81
R7141:Lrrc66	UTSW	5	73,787,320 (GRCm39)	missense	probably benign	0.10
R7201:Lrrc66	UTSW	5	73,787,240 (GRCm39)	missense	probably benign	0.11
R7250:Lrrc66	UTSW	5	73,768,224 (GRCm39)	missense	probably benign	0.03
R7367:Lrrc66	UTSW	5	73,765,724 (GRCm39)	missense	probably benign	0.13
R7773:Lrrc66	UTSW	5	73,764,664 (GRCm39)	missense	probably damaging	0.98
R7925:Lrrc66	UTSW	5	73,765,835 (GRCm39)	missense	possibly damaging	0.81
R8057:Lrrc66	UTSW	5	73,764,875 (GRCm39)	nonsense	probably null
R8167:Lrrc66	UTSW	5	73,786,952 (GRCm39)	nonsense	probably null
R8552:Lrrc66	UTSW	5	73,768,228 (GRCm39)	missense	probably benign	0.16
R8834:Lrrc66	UTSW	5	73,765,928 (GRCm39)	missense	possibly damaging	0.56
R8992:Lrrc66	UTSW	5	73,787,227 (GRCm39)	missense	probably benign	0.04
R9017:Lrrc66	UTSW	5	73,765,244 (GRCm39)	missense	possibly damaging	0.94
R9051:Lrrc66	UTSW	5	73,765,268 (GRCm39)	missense	probably benign	0.00
R9051:Lrrc66	UTSW	5	73,765,267 (GRCm39)	missense	probably benign	0.05

Predicted Primers

PCR Primer
(F):5'- AGCAGTAGACTTCAGTTGTTCCAGGA -3'
(R):5'- TGCTTGTAATGTGTATCCCACCAGGA -3'

Sequencing Primer
(F):5'- GTTCCAGGAGGTCTTAAATAGCAC -3'
(R):5'- TCCCACCAGGAACATTTTGATG -3'

Posted On

2013-07-11