Incidental Mutation 'R7322:Ankrd10'
ID 568219
Institutional Source Beutler Lab
Gene Symbol Ankrd10
Ensembl Gene ENSMUSG00000031508
Gene Name ankyrin repeat domain 10
Synonyms 4833425P12Rik
MMRRC Submission 045417-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.080) question?
Stock # R7322 (G1)
Quality Score 225.009
Status Not validated
Chromosome 8
Chromosomal Location 11661583-11685757 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 11665841 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Valine to Glutamic Acid at position 253 (V253E)
Ref Sequence ENSEMBL: ENSMUSP00000033905 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000033905] [ENSMUST00000169782] [ENSMUST00000209915] [ENSMUST00000210530] [ENSMUST00000211174]
AlphaFold Q99LW0
Predicted Effect probably damaging
Transcript: ENSMUST00000033905
AA Change: V253E

PolyPhen 2 Score 0.974 (Sensitivity: 0.76; Specificity: 0.96)
SMART Domains Protein: ENSMUSP00000033905
Gene: ENSMUSG00000031508
AA Change: V253E

DomainStartEndE-ValueType
Blast:ANK 20 49 2e-10 BLAST
ANK 56 85 2.66e-5 SMART
ANK 90 119 6.46e-4 SMART
ANK 123 152 1.03e-2 SMART
Blast:ANK 156 185 6e-6 BLAST
Predicted Effect probably damaging
Transcript: ENSMUST00000169782
AA Change: V253E

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)
SMART Domains Protein: ENSMUSP00000131657
Gene: ENSMUSG00000031508
AA Change: V253E

DomainStartEndE-ValueType
Blast:ANK 20 49 1e-10 BLAST
ANK 56 85 2.66e-5 SMART
ANK 90 119 6.46e-4 SMART
ANK 123 152 1.03e-2 SMART
Blast:ANK 156 185 4e-6 BLAST
Predicted Effect probably benign
Transcript: ENSMUST00000209915
Predicted Effect probably benign
Transcript: ENSMUST00000210530
Predicted Effect probably benign
Transcript: ENSMUST00000211174
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.0%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 68 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca1 A T 4: 53,067,151 (GRCm39) V1352D probably damaging Het
Adam33 C T 2: 130,895,614 (GRCm39) C567Y probably damaging Het
Alppl2 C A 1: 87,015,184 (GRCm39) G422C probably benign Het
Ang T A 14: 51,338,868 (GRCm39) I3K unknown Het
Arhgef17 T C 7: 100,527,004 (GRCm39) I806V probably benign Het
Atp10b T C 11: 43,103,374 (GRCm39) L586P probably damaging Het
Bdkrb1 T C 12: 105,570,563 (GRCm39) V43A possibly damaging Het
Brinp3 C T 1: 146,558,426 (GRCm39) R117* probably null Het
Brwd1 A T 16: 95,867,319 (GRCm39) M172K probably damaging Het
Bsn G A 9: 108,003,620 (GRCm39) R262* probably null Het
Bst2 A C 8: 71,989,851 (GRCm39) L74R probably damaging Het
C1qa T C 4: 136,623,465 (GRCm39) *246W probably null Het
Cadm2 G A 16: 66,679,734 (GRCm39) T42M probably damaging Het
Ccdc150 A G 1: 54,299,125 (GRCm39) T34A probably benign Het
Ccdc178 G A 18: 22,238,606 (GRCm39) T337M probably benign Het
Ccl8 T C 11: 82,007,408 (GRCm39) V81A probably damaging Het
Dgcr6 A G 16: 17,888,771 (GRCm39) T69A unknown Het
Dnah10 A G 5: 124,898,333 (GRCm39) D3762G probably benign Het
Eif3d T C 15: 77,845,876 (GRCm39) T382A probably benign Het
Epb41 A T 4: 131,717,030 (GRCm39) Y375N probably damaging Het
Epb41l1 A T 2: 156,345,771 (GRCm39) H258L probably damaging Het
Fbn1 A C 2: 125,321,115 (GRCm39) I81S possibly damaging Het
Fzd5 A G 1: 64,774,487 (GRCm39) S425P probably damaging Het
Gfra2 T A 14: 71,205,831 (GRCm39) V398D probably benign Het
Gm3755 A G 14: 18,620,904 (GRCm39) L130P Het
Grin3b A G 10: 79,811,529 (GRCm39) Y705C probably damaging Het
Hmcn2 A G 2: 31,349,093 (GRCm39) D4944G probably damaging Het
Hoxd11 T C 2: 74,514,355 (GRCm39) L295P probably damaging Het
Ighv1-81 C A 12: 115,884,287 (GRCm39) G16C possibly damaging Het
Kcna5 T C 6: 126,510,754 (GRCm39) D458G possibly damaging Het
Klb A T 5: 65,540,707 (GRCm39) E933D probably benign Het
Lrp1 A T 10: 127,381,433 (GRCm39) M3855K probably benign Het
Map3k4 A T 17: 12,489,833 (GRCm39) L533I probably damaging Het
Mrps6 C A 16: 91,855,335 (GRCm39) Y4* probably null Het
Nabp1 A T 1: 51,512,229 (GRCm39) V105E probably damaging Het
Naip6 T A 13: 100,435,896 (GRCm39) N876Y possibly damaging Het
Nlrp2 T C 7: 5,311,644 (GRCm39) S944G possibly damaging Het
Nr4a3 A T 4: 48,083,238 (GRCm39) E590D probably benign Het
Or10h5 A G 17: 33,434,673 (GRCm39) I215T probably damaging Het
Or1b1 T C 2: 36,995,603 (GRCm39) R20G probably null Het
Or52ab2 T A 7: 102,970,494 (GRCm39) V292E Het
Or5j1 A T 2: 86,878,823 (GRCm39) Y252* probably null Het
Or8k22 C T 2: 86,162,908 (GRCm39) S264N probably benign Het
Pcp4l1 G A 1: 171,002,034 (GRCm39) A42V possibly damaging Het
Pdzd2 T C 15: 12,437,248 (GRCm39) D451G probably damaging Het
Pkd2l1 A G 19: 44,146,129 (GRCm39) S142P probably benign Het
Postn T C 3: 54,277,701 (GRCm39) L232P probably damaging Het
Prss8 T C 7: 127,528,735 (GRCm39) T33A probably benign Het
Ptgr3 A G 18: 84,113,260 (GRCm39) Y312C probably damaging Het
Rapgef2 A T 3: 79,053,130 (GRCm39) M1K probably null Het
Rasa3 T C 8: 13,645,857 (GRCm39) N161S possibly damaging Het
Riox1 G A 12: 83,997,442 (GRCm39) probably benign Het
Rmdn2 A T 17: 79,929,040 (GRCm39) K97N probably damaging Het
Sgo1 A G 17: 53,984,085 (GRCm39) L431P probably damaging Het
Slc9a8 T A 2: 167,293,222 (GRCm39) V217E probably damaging Het
Slco2b1 C T 7: 99,341,055 (GRCm39) G21D not run Het
Spata31d1d C A 13: 59,874,790 (GRCm39) R915L probably benign Het
Stk17b A T 1: 53,805,104 (GRCm39) N152K probably benign Het
Syne2 T A 12: 76,030,798 (GRCm39) I3634N probably damaging Het
Tnfrsf9 A G 4: 151,018,794 (GRCm39) D155G probably damaging Het
Tnrc6a T A 7: 122,770,731 (GRCm39) D840E probably benign Het
Tyk2 G A 9: 21,021,500 (GRCm39) S902L probably benign Het
Unc119 T C 11: 78,239,449 (GRCm39) S235P probably damaging Het
Vav1 A C 17: 57,609,266 (GRCm39) D394A probably benign Het
Vezf1 T A 11: 87,972,410 (GRCm39) I439K possibly damaging Het
Zfp335 A T 2: 164,752,741 (GRCm39) M1K probably null Het
Zfp715 T C 7: 42,960,562 (GRCm39) T10A possibly damaging Het
Zmynd11 C T 13: 9,740,445 (GRCm39) E382K possibly damaging Het
Other mutations in Ankrd10
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01480:Ankrd10 APN 8 11,685,592 (GRCm39) missense probably benign
IGL03029:Ankrd10 APN 8 11,669,304 (GRCm39) splice site probably null
R0098:Ankrd10 UTSW 8 11,662,560 (GRCm39) missense probably benign 0.01
R0393:Ankrd10 UTSW 8 11,685,482 (GRCm39) missense possibly damaging 0.58
R2870:Ankrd10 UTSW 8 11,665,682 (GRCm39) missense probably damaging 0.98
R2870:Ankrd10 UTSW 8 11,665,682 (GRCm39) missense probably damaging 0.98
R4176:Ankrd10 UTSW 8 11,662,644 (GRCm39) missense probably benign 0.00
R5985:Ankrd10 UTSW 8 11,669,077 (GRCm39) nonsense probably null
R6999:Ankrd10 UTSW 8 11,669,106 (GRCm39) missense probably damaging 1.00
R7108:Ankrd10 UTSW 8 11,662,624 (GRCm39) missense probably damaging 1.00
R7522:Ankrd10 UTSW 8 11,682,910 (GRCm39) missense probably damaging 1.00
R7559:Ankrd10 UTSW 8 11,662,548 (GRCm39) missense probably damaging 0.97
R7629:Ankrd10 UTSW 8 11,665,769 (GRCm39) missense probably benign 0.13
R8263:Ankrd10 UTSW 8 11,665,707 (GRCm39) missense probably benign 0.22
R8493:Ankrd10 UTSW 8 11,678,518 (GRCm39) nonsense probably null
R8713:Ankrd10 UTSW 8 11,678,491 (GRCm39) missense probably damaging 1.00
R8897:Ankrd10 UTSW 8 11,665,788 (GRCm39) missense possibly damaging 0.61
Predicted Primers PCR Primer
(F):5'- TGATTCTTGCAACGACAAGGGG -3'
(R):5'- ATGTGTGACTTTGAGCTGAAATTGC -3'

Sequencing Primer
(F):5'- CAAGGGGTTTGCATTGCTGAAAC -3'
(R):5'- AAGGTTTACTAGTGATCTTTGCTTC -3'
Posted On 2019-06-26