Mutagenetix > Incidental Mutation

Incidental Mutation 'R7324:Wdfy4'

568769

Institutional Source

Beutler Lab

Gene Symbol

Wdfy4

Ensembl Gene

ENSMUSG00000051506

Gene Name

WD repeat and FYVE domain containing 4

Synonyms

MMRRC Submission

045418-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7324 (G1)

Quality Score

200.009

Status

Validated

Chromosome

Chromosomal Location

32681504-32907465 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 32769271 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 2219 (S2219P)

Ref Sequence

ENSEMBL: ENSMUSP00000117068 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061753] [ENSMUST00000130509]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000061753
AA Change: S2065P

PolyPhen 2 Score 0.231 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000057556
Gene: ENSMUSG00000051506
AA Change: S2065P

Domain	Start	End	E-Value	Type
low complexity region	69	77	N/A	INTRINSIC
low complexity region	204	222	N/A	INTRINSIC
low complexity region	508	522	N/A	INTRINSIC
low complexity region	618	632	N/A	INTRINSIC
low complexity region	644	661	N/A	INTRINSIC
low complexity region	1585	1604	N/A	INTRINSIC
low complexity region	1899	1909	N/A	INTRINSIC
Pfam:PH_BEACH	2237	2348	1.2e-9	PFAM
Beach	2378	2660	3.69e-196	SMART
WD40	2761	2801	1.98e1	SMART
WD40	2811	2850	5.18e-7	SMART
WD40	2853	2891	9.94e-1	SMART
WD40	2893	2940	3.17e-2	SMART
WD40	2986	3021	3.31e0	SMART

Predicted Effect

SMART Domains

Protein: ENSMUSP00000117068
Gene: ENSMUSG00000051506
AA Change: S2219P

Domain	Start	End	E-Value	Type
low complexity region	69	77	N/A	INTRINSIC
low complexity region	204	222	N/A	INTRINSIC
low complexity region	508	522	N/A	INTRINSIC
low complexity region	618	632	N/A	INTRINSIC
low complexity region	644	661	N/A	INTRINSIC
low complexity region	1596	1615	N/A	INTRINSIC
low complexity region	1795	1819	N/A	INTRINSIC
low complexity region	2019	2029	N/A	INTRINSIC
Pfam:PH_BEACH	2362	2473	1.2e-9	PFAM
Beach	2503	2785	3.69e-196	SMART
WD40	2886	2926	1.98e1	SMART
WD40	2936	2975	5.18e-7	SMART
WD40	2978	3016	9.94e-1	SMART
WD40	3018	3065	3.17e-2	SMART
WD40	3111	3146	3.31e0	SMART

Meta Mutation Damage Score

0.2213

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

99% (81/82)

Allele List at MGI

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcg5	T	C	17: 84,983,667 (GRCm39)	D124G	possibly damaging	Het
Ackr3	C	G	1: 90,141,923 (GRCm39)	N127K	probably damaging	Het
AL732309.1	A	C	2: 25,136,151 (GRCm39)	M21R	possibly damaging	Het
Ankrd52	C	A	10: 128,222,032 (GRCm39)	T552K	possibly damaging	Het
Arhgap28	T	C	17: 68,202,879 (GRCm39)		probably null	Het
Arid5b	A	T	10: 67,964,752 (GRCm39)	N306K	probably benign	Het
C1qtnf3	A	C	15: 10,952,707 (GRCm39)	K56N	probably benign	Het
C2	C	T	17: 35,100,664 (GRCm39)	G52D	probably benign	Het
Casz1	C	T	4: 149,031,490 (GRCm39)	T1247M	probably damaging	Het
Cc2d2b	A	G	19: 40,797,552 (GRCm39)	D778G	unknown	Het
Cdh5	A	G	8: 104,869,425 (GRCm39)	D717G	probably damaging	Het
Clca3a2	G	A	3: 144,514,372 (GRCm39)	A445V	probably damaging	Het
Clca3b	T	A	3: 144,547,181 (GRCm39)	M319L	possibly damaging	Het
Csmd1	G	A	8: 16,108,721 (GRCm39)	S1894L	probably damaging	Het
Csnk1g3	A	G	18: 54,052,090 (GRCm39)	T220A	probably damaging	Het
Cyp2d10	T	G	15: 82,287,961 (GRCm39)	T381P	probably damaging	Het
Ddb2	G	A	2: 91,067,229 (GRCm39)		probably benign	Het
Ddx24	C	A	12: 103,382,518 (GRCm39)	L688F	probably damaging	Het
Dennd4c	T	C	4: 86,747,975 (GRCm39)	L1615P	unknown	Het
Dnah8	T	A	17: 31,003,099 (GRCm39)	D3599E	probably benign	Het
Dst	T	C	1: 34,045,305 (GRCm39)	S13P	possibly damaging	Het
Efcab12	T	C	6: 115,800,555 (GRCm39)	D156G	probably benign	Het
Enpp2	A	G	15: 54,741,170 (GRCm39)		probably null	Het
Ephx2	A	G	14: 66,322,803 (GRCm39)	V490A	probably damaging	Het
Etnppl	A	G	3: 130,423,224 (GRCm39)	N308D	probably damaging	Het
F5	TCAGAAGACCTCCTCCCCAGACCTGGGCCAGGTGCCCCTTTCTCCAGATGACAACCAGAAGACCTCCTCCCCAGACCTGGGTCAGGTGTCCCTTTCTCCAGATGATAACCAGAAGACCTCCTCCCCAGACCTGGGTCAGGTGCCCCTTTCTCTAGATGACAACCAGAAGACGACCTCCCCAGACCTGGGTCAGGTGCCCCTTTCTCCAGATGACAACCAGA	TCAGAAGACCTCCTCCCCAGACCTGGGTCAGGTGTCCCTTTCTCCAGATGATAACCAGAAGACCTCCTCCCCAGACCTGGGTCAGGTGCCCCTTTCTCTAGATGACAACCAGAAGACGACCTCCCCAGACCTGGGTCAGGTGCCCCTTTCTCCAGATGACAACCAGA	1: 164,021,150 (GRCm39)		probably benign	Het
Fam162b	A	T	10: 51,466,282 (GRCm39)		probably null	Het
Fancl	A	G	11: 26,353,362 (GRCm39)	E86G	probably damaging	Het
Flii	G	A	11: 60,609,866 (GRCm39)	T615I	probably benign	Het
Fndc7	G	T	3: 108,779,537 (GRCm39)	Q336K	probably benign	Het
Gm26661	T	C	14: 7,791,911 (GRCm38)	C109R	unknown	Het
H2-DMb1	T	C	17: 34,378,436 (GRCm39)		probably null	Het
H2-T10	C	T	17: 36,430,189 (GRCm39)	G251R	probably damaging	Het
Harbi1	T	C	2: 91,551,044 (GRCm39)	I339T	probably benign	Het
Hsp90aa1	A	G	12: 110,661,659 (GRCm39)	M119T	unknown	Het
Ighe	T	A	12: 113,235,954 (GRCm39)	Y124F		Het
Ighv7-1	T	C	12: 113,860,149 (GRCm39)	Y81C	probably damaging	Het
Ilkap	A	T	1: 91,313,115 (GRCm39)		probably null	Het
Inpp5a	A	G	7: 139,105,586 (GRCm39)	D179G	probably damaging	Het
Itgad	A	T	7: 127,788,979 (GRCm39)	D510V	probably damaging	Het
Kcnn2	A	T	18: 45,693,138 (GRCm39)	H238L	probably benign	Het
Kctd17	T	A	15: 78,319,842 (GRCm39)	C189S	probably damaging	Het
Larp4b	C	A	13: 9,208,616 (GRCm39)	A423E	probably benign	Het
Llgl2	A	G	11: 115,741,556 (GRCm39)	E562G	possibly damaging	Het
Macf1	T	C	4: 123,268,218 (GRCm39)	T6734A	probably benign	Het
Maz	G	A	7: 126,623,765 (GRCm39)	T377M	probably damaging	Het
Mmrn1	G	T	6: 60,921,917 (GRCm39)	G125*	probably null	Het
Mvp	A	G	7: 126,592,781 (GRCm39)	S377P	probably benign	Het
Nin	C	T	12: 70,090,508 (GRCm39)	R969Q		Het
Nktr	C	T	9: 121,556,427 (GRCm39)	T35I	probably damaging	Het
Nktr	T	A	9: 121,577,357 (GRCm39)	M475K	possibly damaging	Het
Nod2	T	A	8: 89,379,694 (GRCm39)	V65D	probably damaging	Het
Opa1	A	T	16: 29,405,799 (GRCm39)	E121D	probably benign	Het
Or10w3	C	T	19: 13,704,270 (GRCm39)	A215V	probably benign	Het
Or1r1	A	G	11: 73,874,669 (GRCm39)	V255A	probably benign	Het
Or2n1c	T	C	17: 38,519,607 (GRCm39)	V157A	probably benign	Het
Or4a76	A	T	2: 89,460,447 (GRCm39)	I265N	possibly damaging	Het
Or5ac21	T	C	16: 59,123,611 (GRCm39)	F32L	probably benign	Het
Or5b123	A	G	19: 13,596,942 (GRCm39)	I96V	probably benign	Het
Or6c88	T	A	10: 129,406,718 (GRCm39)	S65T	probably damaging	Het
Or7e174	A	T	9: 20,012,726 (GRCm39)	I224F	possibly damaging	Het
Osbpl2	A	G	2: 179,791,994 (GRCm39)	T233A	probably benign	Het
Pcp4l1	G	A	1: 171,002,034 (GRCm39)	A42V	possibly damaging	Het
Plekhh3	T	A	11: 101,061,600 (GRCm39)	D38V	possibly damaging	Het
Prtg	C	T	9: 72,798,122 (GRCm39)	A696V	probably damaging	Het
Ptpn14	T	G	1: 189,595,621 (GRCm39)	V748G	possibly damaging	Het
Reg2	A	T	6: 78,383,137 (GRCm39)	D28V	probably benign	Het
Rhpn1	A	T	15: 75,576,246 (GRCm39)	I2F	possibly damaging	Het
Rundc3a	A	G	11: 102,290,799 (GRCm39)	E294G	possibly damaging	Het
Scara3	T	A	14: 66,168,865 (GRCm39)	I251L	probably benign	Het
Slc23a2	G	A	2: 131,931,043 (GRCm39)	T152I	probably damaging	Het
Slc39a2	T	G	14: 52,131,650 (GRCm39)	S74A	possibly damaging	Het
Tmprss15	T	C	16: 78,758,907 (GRCm39)	Y937C	probably damaging	Het
Tpp2	T	C	1: 44,017,938 (GRCm39)	L779S	probably damaging	Het
Tssk2	T	C	16: 17,717,227 (GRCm39)	V210A	possibly damaging	Het
Ttn	T	A	2: 76,725,937 (GRCm39)	T6100S	unknown	Het
Tufm	G	A	7: 126,088,759 (GRCm39)	E317K	possibly damaging	Het
Vinac1	T	C	2: 128,879,772 (GRCm39)	D718G	unknown	Het
Wtap	T	C	17: 13,199,833 (GRCm39)	N50S	possibly damaging	Het

Other mutations in Wdfy4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00231:Wdfy4	APN	14	32,824,496 (GRCm39)	missense	possibly damaging	0.93
IGL01116:Wdfy4	APN	14	32,681,934 (GRCm39)	missense	probably damaging	1.00
IGL01449:Wdfy4	APN	14	32,825,994 (GRCm39)	missense	probably damaging	0.99
IGL01567:Wdfy4	APN	14	32,873,618 (GRCm39)	missense	probably benign	0.01
IGL01700:Wdfy4	APN	14	32,742,195 (GRCm39)	splice site	probably benign
IGL01931:Wdfy4	APN	14	32,877,710 (GRCm39)	missense	probably damaging	1.00
IGL01981:Wdfy4	APN	14	32,855,673 (GRCm39)	missense	probably damaging	1.00
IGL01988:Wdfy4	APN	14	32,798,437 (GRCm39)	missense	possibly damaging	0.75
IGL02026:Wdfy4	APN	14	32,815,257 (GRCm39)	missense	probably damaging	1.00
IGL02066:Wdfy4	APN	14	32,871,523 (GRCm39)	missense	probably benign
IGL02468:Wdfy4	APN	14	32,688,389 (GRCm39)	missense	probably benign	0.01
IGL02512:Wdfy4	APN	14	32,764,448 (GRCm39)	missense	probably benign	0.01
IGL02597:Wdfy4	APN	14	32,812,818 (GRCm39)	nonsense	probably null
IGL02752:Wdfy4	APN	14	32,798,283 (GRCm39)	missense	probably damaging	1.00
IGL02792:Wdfy4	APN	14	32,817,262 (GRCm39)	missense	probably benign	0.01
IGL02826:Wdfy4	APN	14	32,693,707 (GRCm39)	missense	possibly damaging	0.47
IGL02903:Wdfy4	APN	14	32,831,607 (GRCm39)	missense	probably damaging	1.00
IGL02955:Wdfy4	APN	14	32,798,241 (GRCm39)	missense	probably damaging	1.00
IGL03031:Wdfy4	APN	14	32,862,608 (GRCm39)	missense	probably damaging	1.00
IGL03102:Wdfy4	APN	14	32,688,392 (GRCm39)	missense	probably damaging	1.00
IGL03123:Wdfy4	APN	14	32,884,827 (GRCm39)	missense	probably benign	0.01
IGL03198:Wdfy4	APN	14	32,847,844 (GRCm39)	missense	probably damaging	1.00
IGL03250:Wdfy4	APN	14	32,699,124 (GRCm39)	missense	probably damaging	0.99
IGL03277:Wdfy4	APN	14	32,790,861 (GRCm39)	missense	probably benign	0.01
IGL03398:Wdfy4	APN	14	32,769,247 (GRCm39)	missense	probably benign	0.14
dodgers	UTSW	14	32,699,063 (GRCm39)	nonsense	probably null
Dollar	UTSW	14	32,742,268 (GRCm39)	missense	probably damaging	1.00
Giants	UTSW	14	32,792,575 (GRCm39)	nonsense	probably null
gigantea	UTSW	14	32,696,111 (GRCm39)	critical splice donor site	probably null
kings_canyon	UTSW	14	32,831,476 (GRCm39)	nonsense	probably null
moro	UTSW	14	32,686,583 (GRCm39)	splice site	probably null
popped	UTSW	14	32,688,356 (GRCm39)	missense	probably damaging	0.99
sequoia	UTSW	14	32,822,860 (GRCm39)	critical splice donor site	probably null
Sherman	UTSW	14	32,817,908 (GRCm39)	missense	possibly damaging	0.89
stretched	UTSW	14	32,795,492 (GRCm39)	nonsense	probably null
watchtower	UTSW	14	32,805,596 (GRCm39)	critical splice donor site	probably null
R0014:Wdfy4	UTSW	14	32,829,130 (GRCm39)	missense	possibly damaging	0.72
R0067:Wdfy4	UTSW	14	32,884,708 (GRCm39)	missense	probably null	1.00
R0085:Wdfy4	UTSW	14	32,800,200 (GRCm39)	missense	possibly damaging	0.81
R0277:Wdfy4	UTSW	14	32,805,742 (GRCm39)	missense	possibly damaging	0.83
R0436:Wdfy4	UTSW	14	32,805,769 (GRCm39)	splice site	probably benign
R0496:Wdfy4	UTSW	14	32,862,695 (GRCm39)	splice site	probably benign
R0514:Wdfy4	UTSW	14	32,802,732 (GRCm39)	missense	probably benign	0.22
R0548:Wdfy4	UTSW	14	32,764,578 (GRCm39)	missense	probably benign
R0590:Wdfy4	UTSW	14	32,763,131 (GRCm39)	missense	probably benign	0.09
R0647:Wdfy4	UTSW	14	32,831,656 (GRCm39)	missense	possibly damaging	0.96
R0766:Wdfy4	UTSW	14	32,862,569 (GRCm39)	missense	probably damaging	1.00
R0981:Wdfy4	UTSW	14	32,869,049 (GRCm39)	missense	probably benign	0.03
R1024:Wdfy4	UTSW	14	32,801,923 (GRCm39)	missense	possibly damaging	0.81
R1113:Wdfy4	UTSW	14	32,693,695 (GRCm39)	missense	possibly damaging	0.47
R1252:Wdfy4	UTSW	14	32,693,729 (GRCm39)	splice site	probably null
R1415:Wdfy4	UTSW	14	32,763,137 (GRCm39)	missense	possibly damaging	0.60
R1475:Wdfy4	UTSW	14	32,830,645 (GRCm39)	missense	probably benign	0.14
R1483:Wdfy4	UTSW	14	32,822,923 (GRCm39)	missense	probably benign	0.41
R1490:Wdfy4	UTSW	14	32,874,495 (GRCm39)	critical splice donor site	probably null
R1512:Wdfy4	UTSW	14	32,682,765 (GRCm39)	missense	probably damaging	0.98
R1615:Wdfy4	UTSW	14	32,764,469 (GRCm39)	missense	probably damaging	1.00
R1628:Wdfy4	UTSW	14	32,681,918 (GRCm39)	missense	probably damaging	1.00
R1643:Wdfy4	UTSW	14	32,795,542 (GRCm39)	critical splice acceptor site	probably null
R1729:Wdfy4	UTSW	14	32,817,962 (GRCm39)	missense	possibly damaging	0.85
R1859:Wdfy4	UTSW	14	32,825,940 (GRCm39)	missense	probably damaging	0.99
R1933:Wdfy4	UTSW	14	32,855,301 (GRCm39)	missense	probably benign	0.08
R1957:Wdfy4	UTSW	14	32,693,641 (GRCm39)	missense	probably damaging	1.00
R1968:Wdfy4	UTSW	14	32,828,001 (GRCm39)	missense	possibly damaging	0.95
R2032:Wdfy4	UTSW	14	32,868,946 (GRCm39)	missense	probably benign	0.11
R2241:Wdfy4	UTSW	14	32,795,468 (GRCm39)	missense	possibly damaging	0.81
R2391:Wdfy4	UTSW	14	32,884,764 (GRCm39)	missense	possibly damaging	0.92
R2888:Wdfy4	UTSW	14	32,831,476 (GRCm39)	nonsense	probably null
R2889:Wdfy4	UTSW	14	32,831,476 (GRCm39)	nonsense	probably null
R3114:Wdfy4	UTSW	14	32,811,860 (GRCm39)	missense	probably damaging	0.97
R3757:Wdfy4	UTSW	14	32,745,331 (GRCm39)	missense	probably benign	0.17
R3758:Wdfy4	UTSW	14	32,745,331 (GRCm39)	missense	probably benign	0.17
R3797:Wdfy4	UTSW	14	32,862,602 (GRCm39)	missense	probably damaging	1.00
R3890:Wdfy4	UTSW	14	32,769,237 (GRCm39)	missense	probably damaging	1.00
R3892:Wdfy4	UTSW	14	32,769,237 (GRCm39)	missense	probably damaging	1.00
R3945:Wdfy4	UTSW	14	32,688,352 (GRCm39)	missense	probably damaging	0.99
R4011:Wdfy4	UTSW	14	32,824,637 (GRCm39)	splice site	probably benign
R4091:Wdfy4	UTSW	14	32,847,837 (GRCm39)	missense	possibly damaging	0.93
R4449:Wdfy4	UTSW	14	32,818,040 (GRCm39)	missense	probably damaging	1.00
R4585:Wdfy4	UTSW	14	32,809,912 (GRCm39)	missense	possibly damaging	0.89
R4628:Wdfy4	UTSW	14	32,824,515 (GRCm39)	missense	probably damaging	0.97
R4629:Wdfy4	UTSW	14	32,824,515 (GRCm39)	missense	probably damaging	0.97
R4655:Wdfy4	UTSW	14	32,711,893 (GRCm39)	missense	probably damaging	0.98
R4689:Wdfy4	UTSW	14	32,831,505 (GRCm39)	missense	possibly damaging	0.88
R4718:Wdfy4	UTSW	14	32,867,273 (GRCm39)	missense	probably benign	0.03
R4862:Wdfy4	UTSW	14	32,822,860 (GRCm39)	critical splice donor site	probably null
R4884:Wdfy4	UTSW	14	32,710,852 (GRCm39)	nonsense	probably null
R4894:Wdfy4	UTSW	14	32,877,717 (GRCm39)	missense	probably benign	0.03
R4929:Wdfy4	UTSW	14	32,769,213 (GRCm39)	missense	possibly damaging	0.90
R4932:Wdfy4	UTSW	14	32,750,970 (GRCm39)	missense	probably damaging	1.00
R5014:Wdfy4	UTSW	14	32,822,897 (GRCm39)	missense	probably benign	0.02
R5020:Wdfy4	UTSW	14	32,801,892 (GRCm39)	missense	probably damaging	1.00
R5049:Wdfy4	UTSW	14	32,874,627 (GRCm39)	missense	possibly damaging	0.78
R5276:Wdfy4	UTSW	14	32,769,232 (GRCm39)	missense	probably damaging	1.00
R5318:Wdfy4	UTSW	14	32,800,300 (GRCm39)	missense	possibly damaging	0.95
R5338:Wdfy4	UTSW	14	32,812,823 (GRCm39)	missense	probably damaging	1.00
R5349:Wdfy4	UTSW	14	32,710,856 (GRCm39)	missense	probably damaging	1.00
R5411:Wdfy4	UTSW	14	32,681,959 (GRCm39)	missense	probably damaging	1.00
R5435:Wdfy4	UTSW	14	32,742,268 (GRCm39)	missense	probably damaging	1.00
R5463:Wdfy4	UTSW	14	32,873,689 (GRCm39)	missense	probably benign	0.17
R5591:Wdfy4	UTSW	14	32,829,087 (GRCm39)	missense	probably benign	0.09
R5598:Wdfy4	UTSW	14	32,855,454 (GRCm39)	missense	probably damaging	1.00
R5654:Wdfy4	UTSW	14	32,829,575 (GRCm39)	splice site	probably null
R5890:Wdfy4	UTSW	14	32,824,534 (GRCm39)	missense	possibly damaging	0.91
R5894:Wdfy4	UTSW	14	32,855,317 (GRCm39)	missense	possibly damaging	0.86
R5964:Wdfy4	UTSW	14	32,827,968 (GRCm39)	missense	probably damaging	1.00
R6036:Wdfy4	UTSW	14	32,868,947 (GRCm39)	missense	probably damaging	0.97
R6036:Wdfy4	UTSW	14	32,868,947 (GRCm39)	missense	probably damaging	0.97
R6074:Wdfy4	UTSW	14	32,805,596 (GRCm39)	critical splice donor site	probably null
R6135:Wdfy4	UTSW	14	32,693,668 (GRCm39)	missense	probably damaging	0.99
R6276:Wdfy4	UTSW	14	32,831,482 (GRCm39)	missense	possibly damaging	0.54
R6357:Wdfy4	UTSW	14	32,823,006 (GRCm39)	nonsense	probably null
R6370:Wdfy4	UTSW	14	32,790,807 (GRCm39)	missense	probably benign	0.16
R6390:Wdfy4	UTSW	14	32,826,051 (GRCm39)	missense	probably damaging	0.99
R6413:Wdfy4	UTSW	14	32,689,604 (GRCm39)	missense	probably damaging	1.00
R6450:Wdfy4	UTSW	14	32,830,649 (GRCm39)	missense	probably damaging	1.00
R6522:Wdfy4	UTSW	14	32,868,901 (GRCm39)	missense	probably damaging	0.98
R6657:Wdfy4	UTSW	14	32,769,208 (GRCm39)	missense	possibly damaging	0.70
R6761:Wdfy4	UTSW	14	32,817,908 (GRCm39)	missense	possibly damaging	0.89
R6763:Wdfy4	UTSW	14	32,764,469 (GRCm39)	missense	probably damaging	1.00
R6952:Wdfy4	UTSW	14	32,681,923 (GRCm39)	missense	probably damaging	1.00
R6985:Wdfy4	UTSW	14	32,821,074 (GRCm39)	missense	possibly damaging	0.68
R7024:Wdfy4	UTSW	14	32,686,583 (GRCm39)	splice site	probably null
R7101:Wdfy4	UTSW	14	32,682,777 (GRCm39)	missense
R7114:Wdfy4	UTSW	14	32,693,531 (GRCm39)	splice site	probably null
R7139:Wdfy4	UTSW	14	32,873,535 (GRCm39)	missense
R7255:Wdfy4	UTSW	14	32,696,239 (GRCm39)	missense
R7379:Wdfy4	UTSW	14	32,873,566 (GRCm39)	missense
R7399:Wdfy4	UTSW	14	32,790,863 (GRCm39)	missense
R7408:Wdfy4	UTSW	14	32,800,264 (GRCm39)	missense
R7410:Wdfy4	UTSW	14	32,696,191 (GRCm39)	missense
R7411:Wdfy4	UTSW	14	32,828,088 (GRCm39)	missense
R7412:Wdfy4	UTSW	14	32,871,541 (GRCm39)	missense
R7445:Wdfy4	UTSW	14	32,792,575 (GRCm39)	nonsense	probably null
R7595:Wdfy4	UTSW	14	32,696,111 (GRCm39)	critical splice donor site	probably null
R7618:Wdfy4	UTSW	14	32,707,696 (GRCm39)	missense
R7622:Wdfy4	UTSW	14	32,800,231 (GRCm39)	missense
R7828:Wdfy4	UTSW	14	32,710,878 (GRCm39)	missense	possibly damaging	0.90
R7888:Wdfy4	UTSW	14	32,812,920 (GRCm39)	missense
R7946:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R7946:Wdfy4	UTSW	14	32,792,705 (GRCm39)	missense
R7986:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R7990:Wdfy4	UTSW	14	32,819,752 (GRCm39)	missense
R8001:Wdfy4	UTSW	14	32,695,492 (GRCm39)	critical splice donor site	probably null
R8010:Wdfy4	UTSW	14	32,693,584 (GRCm39)	missense
R8015:Wdfy4	UTSW	14	32,829,704 (GRCm39)	missense
R8032:Wdfy4	UTSW	14	32,751,043 (GRCm39)	nonsense	probably null
R8041:Wdfy4	UTSW	14	32,875,965 (GRCm39)	critical splice donor site	probably null
R8090:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8092:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8112:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8114:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8115:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8117:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8117:Wdfy4	UTSW	14	32,699,063 (GRCm39)	nonsense	probably null
R8118:Wdfy4	UTSW	14	32,826,072 (GRCm39)	missense
R8140:Wdfy4	UTSW	14	32,864,317 (GRCm39)	missense
R8155:Wdfy4	UTSW	14	32,884,776 (GRCm39)	missense
R8163:Wdfy4	UTSW	14	32,873,545 (GRCm39)	missense
R8293:Wdfy4	UTSW	14	32,696,218 (GRCm39)	missense
R8325:Wdfy4	UTSW	14	32,689,444 (GRCm39)	missense
R8353:Wdfy4	UTSW	14	32,695,581 (GRCm39)	missense	probably benign
R8370:Wdfy4	UTSW	14	32,815,208 (GRCm39)	missense
R8437:Wdfy4	UTSW	14	32,798,332 (GRCm39)	missense
R8497:Wdfy4	UTSW	14	32,688,356 (GRCm39)	missense	probably damaging	0.99
R8545:Wdfy4	UTSW	14	32,800,258 (GRCm39)	missense	probably benign	0.01
R8671:Wdfy4	UTSW	14	32,693,722 (GRCm39)	splice site	probably benign
R8708:Wdfy4	UTSW	14	32,689,489 (GRCm39)	missense
R8747:Wdfy4	UTSW	14	32,874,611 (GRCm39)	missense
R8794:Wdfy4	UTSW	14	32,869,049 (GRCm39)	missense	probably benign	0.03
R8846:Wdfy4	UTSW	14	32,867,105 (GRCm39)	missense
R8880:Wdfy4	UTSW	14	32,795,492 (GRCm39)	nonsense	probably null
R9109:Wdfy4	UTSW	14	32,760,704 (GRCm39)	splice site	probably null
R9131:Wdfy4	UTSW	14	32,819,807 (GRCm39)	missense
R9309:Wdfy4	UTSW	14	32,817,313 (GRCm39)	missense
R9349:Wdfy4	UTSW	14	32,875,996 (GRCm39)	missense
R9451:Wdfy4	UTSW	14	32,855,518 (GRCm39)	missense
R9563:Wdfy4	UTSW	14	32,692,833 (GRCm39)	missense
R9587:Wdfy4	UTSW	14	32,769,230 (GRCm39)	nonsense	probably null
R9599:Wdfy4	UTSW	14	32,855,428 (GRCm39)	missense
R9670:Wdfy4	UTSW	14	32,769,219 (GRCm39)	missense
R9718:Wdfy4	UTSW	14	32,847,893 (GRCm39)	missense
R9742:Wdfy4	UTSW	14	32,809,987 (GRCm39)	missense
X0028:Wdfy4	UTSW	14	32,802,593 (GRCm39)	missense	probably benign
X0053:Wdfy4	UTSW	14	32,884,899 (GRCm39)	start codon destroyed	probably null	0.99
X0062:Wdfy4	UTSW	14	32,829,575 (GRCm39)	splice site	probably null
Z1177:Wdfy4	UTSW	14	32,809,942 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- ACAAAGGCCCTCTGATTTTCTC -3'
(R):5'- GCCGAGGCTACATTTTGGTG -3'

Sequencing Primer
(F):5'- AAAGGCCCTCTGATTTTCTCTCCTC -3'
(R):5'- TTAAAGAGGAGGGCTGAGTCTCTC -3'

Posted On

2019-09-13