Mutagenetix > Incidental Mutation

Incidental Mutation 'R7332:Vmn2r61'

569298

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r61

Ensembl Gene

ENSMUSG00000090967

Gene Name

vomeronasal 2, receptor 61

Synonyms

EG637873, Casr-rs2, Gprc2a-rs2

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.120) question?

Stock #

R7332 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

42260053-42300755 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 42260110 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 20 (T20A)

Ref Sequence

ENSEMBL: ENSMUSP00000129576 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166131]

AlphaFold

L7N2B8

Predicted Effect

probably benign
Transcript: ENSMUST00000166131
AA Change: T20A

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000129576
Gene: ENSMUSG00000090967
AA Change: T20A

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	4e-42	PFAM
Pfam:NCD3G	514	567	1.9e-21	PFAM
Pfam:7tm_3	600	835	6.2e-52	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (68/68)

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Afap1l2	A	G	19: 56,918,121	S449P	probably damaging	Het
Atp6v0c	A	C	17: 24,169,224	S21A	probably benign	Het
Cacna1e	T	C	1: 154,725,801	Y40C	possibly damaging	Het
Cdadc1	A	G	14: 59,575,764	I398T	possibly damaging	Het
Cfap44	T	A	16: 44,429,828	D756E	probably damaging	Het
Clcnkb	A	T	4: 141,413,932	L104Q	probably null	Het
Clk1	G	A	1: 58,412,694	H421Y	probably benign	Het
Cmpk2	A	T	12: 26,478,062	D426V	probably damaging	Het
Cmya5	A	G	13: 93,092,553	L2009S	possibly damaging	Het
Col5a2	C	G	1: 45,380,165	D1252H	probably damaging	Het
Coro1b	A	G	19: 4,149,357	K5R	probably benign	Het
Csmd2	A	G	4: 128,419,567	T1346A		Het
Csrp1	G	T	1: 135,739,411	W4L	probably benign	Het
Cyp3a25	A	G	5: 145,993,007	I184T	probably damaging	Het
Egfl7	C	A	2: 26,590,713	R128S	probably benign	Het
Fggy	A	T	4: 95,623,482	N157I	probably damaging	Het
Gldc	A	T	19: 30,116,526	L697Q	probably damaging	Het
Gm10436	T	C	12: 88,176,417	T339A	possibly damaging	Het
Gm4952	T	A	19: 12,627,009	Y262N	probably damaging	Het
Gsap	T	A	5: 21,290,121	M821K	probably benign	Het
Hps1	T	C	19: 42,777,912		probably null	Het
Hsbp1l1	T	C	18: 80,236,823		probably benign	Het
Igfbp7	G	A	5: 77,351,956	T220I	probably damaging	Het
Ints3	T	C	3: 90,415,512	Q137R	probably damaging	Het
Kcna6	A	G	6: 126,739,329	F199S	possibly damaging	Het
Kirrel	T	C	3: 87,088,398	I410V	probably benign	Het
Llgl2	T	C	11: 115,848,299	V332A	probably damaging	Het
Lrrc27	A	G	7: 139,242,745	I517M	probably damaging	Het
Mas1	T	C	17: 12,842,219	T106A	probably benign	Het
Mast4	T	A	13: 102,751,424	Y1159F	possibly damaging	Het
Mmp2	A	G	8: 92,850,152	D601G	probably damaging	Het
Mycbp2	A	G	14: 103,156,453	S2891P	probably damaging	Het
Mycbp2	T	A	14: 103,197,357	I2217F	probably damaging	Het
Naip6	A	G	13: 100,300,701	V438A	possibly damaging	Het
Olfr1284	A	G	2: 111,379,393	H131R	not run	Het
Pdf	T	C	8: 107,048,541	R20G	probably benign	Het
Pfdn2	T	C	1: 171,356,594	L47P	probably damaging	Het
Pik3c2g	C	A	6: 139,896,255	N795K		Het
Ppip5k1	A	T	2: 121,311,969	V1333D	probably damaging	Het
Prss44	A	T	9: 110,815,462	I213F	probably damaging	Het
Rbm47	A	G	5: 66,026,214	Y349H	probably damaging	Het
Rcl1	A	T	19: 29,130,696	T253S	probably benign	Het
Rdh1	A	G	10: 127,759,885		probably benign	Het
Scn7a	C	T	2: 66,692,554	W935*	probably null	Het
Sec24b	T	C	3: 130,041,393	N52S	probably benign	Het
Serpinb5	A	T	1: 106,872,361	I94L	probably benign	Het
Setx	T	C	2: 29,146,626	V1041A	probably benign	Het
Slco3a1	G	A	7: 74,318,484	A496V	possibly damaging	Het
Spag5	T	A	11: 78,313,379	L486*	probably null	Het
Spink5	T	C	18: 43,982,250	I183T	probably damaging	Het
Srd5a1	T	C	13: 69,611,054	Y65C	probably benign	Het
Ssh2	T	A	11: 77,453,523	I778N	possibly damaging	Het
Sstr1	C	T	12: 58,213,386	S265L	probably damaging	Het
Syne2	T	A	12: 75,967,755		probably null	Het
Tctn1	A	T	5: 122,261,484	D92E	probably damaging	Het
Tiam2	A	G	17: 3,453,369	I940M	probably damaging	Het
Tmeff2	T	C	1: 50,979,440	W194R	unknown	Het
Tomm20	T	C	8: 126,937,153	T94A	probably benign	Het
Ucn2	A	G	9: 108,986,464	N98S	probably benign	Het
V1rd19	A	T	7: 24,003,318	I70L	probably benign	Het
Vmn1r18	A	G	6: 57,390,518	L17P	probably benign	Het
Wdr59	G	A	8: 111,494,354	T182I		Het
Zfp40	A	T	17: 23,176,181	C477*	probably null	Het
Zfp68	A	G	5: 138,606,568	S498P	possibly damaging	Het
Zfp729b	T	C	13: 67,609,636		probably null	Het
Zfp846	T	G	9: 20,594,225	N460K	probably benign	Het
Zim1	T	C	7: 6,677,353	Y437C	probably damaging	Het

Other mutations in Vmn2r61

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00158:Vmn2r61	APN	7	42300751	missense	possibly damaging	0.96
IGL00824:Vmn2r61	APN	7	42267014	missense	probably benign	0.03
IGL00903:Vmn2r61	APN	7	42300511	missense	probably damaging	1.00
IGL01125:Vmn2r61	APN	7	42260126	missense	probably damaging	1.00
IGL01393:Vmn2r61	APN	7	42266834	missense	probably benign	0.08
IGL01712:Vmn2r61	APN	7	42260237	missense	probably damaging	0.98
IGL01822:Vmn2r61	APN	7	42300706	missense	probably benign	0.18
IGL01835:Vmn2r61	APN	7	42300591	missense	probably benign	0.12
IGL01844:Vmn2r61	APN	7	42260215	missense	probably benign	0.00
IGL01953:Vmn2r61	APN	7	42300189	missense	probably damaging	1.00
IGL02032:Vmn2r61	APN	7	42300042	missense	probably damaging	0.99
IGL02054:Vmn2r61	APN	7	42276734	critical splice donor site	probably null
IGL02569:Vmn2r61	APN	7	42276646	missense	probably damaging	1.00
IGL02697:Vmn2r61	APN	7	42275468	missense	possibly damaging	0.55
IGL02958:Vmn2r61	APN	7	42299937	missense	probably benign
IGL03290:Vmn2r61	APN	7	42265984	missense	probably benign	0.00
IGL03337:Vmn2r61	APN	7	42267085	missense	possibly damaging	0.58
IGL03369:Vmn2r61	APN	7	42260093	missense	probably benign
IGL03402:Vmn2r61	APN	7	42260255	missense	probably benign
R0026:Vmn2r61	UTSW	7	42275474	missense	possibly damaging	0.64
R0319:Vmn2r61	UTSW	7	42300517	missense	probably damaging	0.99
R0433:Vmn2r61	UTSW	7	42265911	missense	probably benign	0.02
R0555:Vmn2r61	UTSW	7	42266018	missense	probably benign	0.02
R0691:Vmn2r61	UTSW	7	42300420	missense	probably damaging	1.00
R1701:Vmn2r61	UTSW	7	42300511	missense	probably damaging	1.00
R1718:Vmn2r61	UTSW	7	42300697	missense	probably benign
R1835:Vmn2r61	UTSW	7	42266652	nonsense	probably null
R1920:Vmn2r61	UTSW	7	42300286	missense	possibly damaging	0.73
R2069:Vmn2r61	UTSW	7	42300001	missense	probably benign	0.06
R2326:Vmn2r61	UTSW	7	42266863	missense	probably damaging	1.00
R2402:Vmn2r61	UTSW	7	42300105	missense	possibly damaging	0.90
R3103:Vmn2r61	UTSW	7	42266643	missense	possibly damaging	0.73
R3107:Vmn2r61	UTSW	7	42267067	missense	possibly damaging	0.82
R4426:Vmn2r61	UTSW	7	42300733	missense	probably benign
R4426:Vmn2r61	UTSW	7	42300735	missense	probably benign
R4484:Vmn2r61	UTSW	7	42300696	missense	probably benign
R4748:Vmn2r61	UTSW	7	42267141	missense	probably damaging	0.96
R4835:Vmn2r61	UTSW	7	42267035	missense	possibly damaging	0.52
R4863:Vmn2r61	UTSW	7	42300708	missense	probably benign	0.03
R4923:Vmn2r61	UTSW	7	42267096	missense	probably damaging	1.00
R4968:Vmn2r61	UTSW	7	42300054	missense	probably benign	0.14
R5114:Vmn2r61	UTSW	7	42300529	missense	possibly damaging	0.92
R5297:Vmn2r61	UTSW	7	42260222	missense	probably benign
R5497:Vmn2r61	UTSW	7	42275482	missense	possibly damaging	0.95
R5508:Vmn2r61	UTSW	7	42266818	missense	possibly damaging	0.52
R5587:Vmn2r61	UTSW	7	42300487	missense	probably damaging	1.00
R5615:Vmn2r61	UTSW	7	42267253	missense	probably benign	0.00
R5615:Vmn2r61	UTSW	7	42300493	missense	probably damaging	1.00
R5782:Vmn2r61	UTSW	7	42299829	missense	probably damaging	1.00
R6136:Vmn2r61	UTSW	7	42267031	missense	probably damaging	1.00
R6207:Vmn2r61	UTSW	7	42260192	missense	probably benign	0.01
R6265:Vmn2r61	UTSW	7	42266491	missense	probably benign	0.01
R6272:Vmn2r61	UTSW	7	42299818	missense	probably damaging	1.00
R6355:Vmn2r61	UTSW	7	42267235	missense	probably benign	0.00
R6469:Vmn2r61	UTSW	7	42265859	nonsense	probably null
R6554:Vmn2r61	UTSW	7	42276715	missense	probably damaging	1.00
R6699:Vmn2r61	UTSW	7	42300156	missense	probably benign
R6768:Vmn2r61	UTSW	7	42300324	missense	probably damaging	1.00
R6824:Vmn2r61	UTSW	7	42299979	missense	probably benign	0.10
R6930:Vmn2r61	UTSW	7	42299940	missense	probably benign	0.02
R7053:Vmn2r61	UTSW	7	42267133	missense	probably damaging	0.96
R7238:Vmn2r61	UTSW	7	42267205	missense	possibly damaging	0.73
R7359:Vmn2r61	UTSW	7	42265983	missense	probably benign	0.11
R7553:Vmn2r61	UTSW	7	42266781	missense	not run
R7710:Vmn2r61	UTSW	7	42267048	missense	probably damaging	1.00
R7732:Vmn2r61	UTSW	7	42266673	missense	probably benign
R7839:Vmn2r61	UTSW	7	42266608	missense	probably damaging	0.97
R7916:Vmn2r61	UTSW	7	42300511	missense	probably damaging	1.00
R8026:Vmn2r61	UTSW	7	42266717	missense	probably benign	0.02
R8440:Vmn2r61	UTSW	7	42266656	missense	probably benign	0.02
R8499:Vmn2r61	UTSW	7	42300276	missense	probably damaging	0.99
R8771:Vmn2r61	UTSW	7	42266770	missense	probably damaging	0.99
R8847:Vmn2r61	UTSW	7	42300586	missense	probably damaging	1.00
R8986:Vmn2r61	UTSW	7	42265901	nonsense	probably null
R9290:Vmn2r61	UTSW	7	42265961	missense	probably benign	0.27
R9311:Vmn2r61	UTSW	7	42300668	missense	possibly damaging	0.92
R9324:Vmn2r61	UTSW	7	42267195	missense	probably benign	0.00
R9476:Vmn2r61	UTSW	7	42300169	missense	probably damaging	1.00
R9521:Vmn2r61	UTSW	7	42267202	missense	probably damaging	0.99
R9619:Vmn2r61	UTSW	7	42276712	missense	probably damaging	0.98
R9729:Vmn2r61	UTSW	7	42300493	missense	probably damaging	1.00
Z1088:Vmn2r61	UTSW	7	42299964	missense	possibly damaging	0.93
Z1176:Vmn2r61	UTSW	7	42260161	missense	possibly damaging	0.46
Z1176:Vmn2r61	UTSW	7	42266742	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AGCTGCACTCATTAACAGTCAG -3'
(R):5'- CTGGTGTTACATGCATGACAAAG -3'

Sequencing Primer
(F):5'- TGCACTCATTAACAGTCAGAATCAC -3'
(R):5'- CCTTTTACAGAGGAAAAGAAGTCAAC -3'

Posted On

2019-09-13