Mutagenetix > Incidental Mutation

Incidental Mutation 'R7341:Frem2'

569827

Institutional Source

Beutler Lab

Gene Symbol

Frem2

Ensembl Gene

ENSMUSG00000037016

Gene Name

Fras1 related extracellular matrix protein 2

Synonyms

my, ne, 6030440P17Rik, b2b1562Clo, 8430406N05Rik

MMRRC Submission

045431-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7341 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

53513938-53657355 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 53654495 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Serine at position 864 (G864S)

Ref Sequence

ENSEMBL: ENSMUSP00000088670 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000091137]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000091137
AA Change: G864S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000088670
Gene: ENSMUSG00000037016
AA Change: G864S

Domain	Start	End	E-Value	Type
signal peptide	1	39	N/A	INTRINSIC
Pfam:Cadherin_3	249	388	4.3e-9	PFAM
Pfam:Cadherin_3	376	532	3e-34	PFAM
Pfam:Cadherin_3	516	665	7.5e-24	PFAM
Pfam:Cadherin_3	632	798	1.6e-21	PFAM
Pfam:Cadherin_3	763	910	1.2e-25	PFAM
Pfam:Cadherin_3	879	1027	5.1e-18	PFAM
Pfam:Cadherin_3	1015	1159	2.2e-20	PFAM
CA	1202	1293	4.8e-1	SMART
Pfam:Cadherin_3	1392	1503	9.8e-24	PFAM
Pfam:Cadherin_3	1504	1612	6.2e-28	PFAM
Pfam:Cadherin_3	1613	1743	5.3e-20	PFAM
Calx_beta	1748	1847	1.5e-5	SMART
Calx_beta	1860	1971	9.47e-12	SMART
Calx_beta	1985	2092	1.65e-11	SMART
Calx_beta	2105	2209	1.99e-5	SMART
Calx_beta	2227	2331	6.9e-14	SMART
transmembrane domain	3103	3125	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

100% (92/92)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an integral membrane protein containing numerous CSPG (chondroitin sulfate proteoglycan element) repeats and Calx-beta domains. The encoded protein localizes to the basement membrane, forming a ternary complex that plays a role in epidermal-dermal interactions. This protein is important for the integrity of skin and renal epithelia. Mutations in this gene are associated with Fraser syndrome. [provided by RefSeq, Apr 2014]
PHENOTYPE: Mice homozygous for mutations at this locus display a significant amount of embryonic lethality due to hemorrhaging of embryonic blisters. Kidney development is severely affected and syndactyly is common. Phenotypes of homozygous mutants are indistinguishable from those of Fras1 homozygous mutant. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 90 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb11	C	T	2: 69,299,867 (GRCm38)	D282N	probably damaging	Het
Adam3	T	A	8: 24,687,980 (GRCm38)	N639I	possibly damaging	Het
Adamtsl3	T	A	7: 82,556,874 (GRCm38)	C756S	probably damaging	Het
AI314180	A	T	4: 58,809,415 (GRCm38)	V1653D	possibly damaging	Het
Arhgap31	C	T	16: 38,712,514 (GRCm38)		probably null	Het
Ascl4	G	A	10: 85,928,665 (GRCm38)	A59T	possibly damaging	Het
Ash1l	T	A	3: 88,981,759 (GRCm38)	I315N	possibly damaging	Het
Atp6v0d2	T	C	4: 19,887,330 (GRCm38)	D206G	possibly damaging	Het
Baz1b	T	A	5: 135,223,116 (GRCm38)	L923I	probably damaging	Het
BC067074	G	T	13: 113,318,172 (GRCm38)	G251C		Het
Birc6	T	A	17: 74,612,074 (GRCm38)	N1966K	probably damaging	Het
Bod1l	T	C	5: 41,788,857 (GRCm38)	N3025S	probably benign	Het
Bub1b	C	A	2: 118,636,786 (GRCm38)	H863Q	possibly damaging	Het
C1rb	T	A	6: 124,577,452 (GRCm38)	Y397*	probably null	Het
Ccdc180	A	G	4: 45,898,644 (GRCm38)	E164G	possibly damaging	Het
Ccdc9	C	T	7: 16,280,627 (GRCm38)	V230M	probably damaging	Het
Cchcr1	C	A	17: 35,526,713 (GRCm38)	N437K	probably benign	Het
Cdhr2	T	A	13: 54,719,492 (GRCm38)	L408Q	probably damaging	Het
Cep89	G	A	7: 35,429,928 (GRCm38)	R630H	probably damaging	Het
Chd1	T	C	17: 15,770,237 (GRCm38)	S1684P	probably damaging	Het
CN725425	A	G	15: 91,242,670 (GRCm38)	Y221C	possibly damaging	Het
Col4a2	T	C	8: 11,398,678 (GRCm38)	V66A	probably damaging	Het
Cpd	C	A	11: 76,846,953 (GRCm38)	R5L	unknown	Het
Cpeb4	C	T	11: 31,918,807 (GRCm38)	T422I	possibly damaging	Het
Cyp1a1	G	T	9: 57,700,824 (GRCm38)	R245L	probably damaging	Het
Cyp4f39	A	G	17: 32,486,954 (GRCm38)	T340A	probably damaging	Het
Cyp4v3	A	T	8: 45,321,750 (GRCm38)	F122L	probably benign	Het
Drc1	T	A	5: 30,364,468 (GRCm38)	L728Q	probably damaging	Het
Dsel	C	T	1: 111,861,573 (GRCm38)	G411S	probably damaging	Het
Ermp1	T	A	19: 29,646,254 (GRCm38)	H112L	probably benign	Het
Exoc8	T	C	8: 124,896,581 (GRCm38)	D349G	probably damaging	Het
Fam151a	T	A	4: 106,735,510 (GRCm38)	M63K	probably benign	Het
Fmo9	C	A	1: 166,676,546 (GRCm38)	G149V	probably damaging	Het
Fndc8	A	G	11: 82,898,777 (GRCm38)	T254A	possibly damaging	Het
Foxi3	G	A	6: 70,960,878 (GRCm38)	V365I	probably benign	Het
Fstl5	T	A	3: 76,482,397 (GRCm38)		probably null	Het
Garnl3	T	A	2: 33,034,129 (GRCm38)	E247V	probably damaging	Het
Gdi1	G	A	X: 74,306,855 (GRCm38)	R55H	probably benign	Het
Gorasp1	T	A	9: 119,937,534 (GRCm38)		probably benign	Het
Hcar2	T	C	5: 123,864,478 (GRCm38)	N321D	possibly damaging	Het
Hoxb6	G	T	11: 96,299,569 (GRCm38)	M131I	probably damaging	Het
Kif13a	A	G	13: 46,826,745 (GRCm38)	V177A	probably damaging	Het
Limch1	C	T	5: 67,034,202 (GRCm38)	T794I	probably benign	Het
Lmbrd2	A	G	15: 9,165,819 (GRCm38)	Y260C	possibly damaging	Het
Lmo7	A	G	14: 101,885,512 (GRCm38)	N379S	probably benign	Het
Lrp6	G	T	6: 134,450,818 (GRCm38)	P1604T	probably damaging	Het
Lrrc14	A	T	15: 76,714,511 (GRCm38)	E449V	probably damaging	Het
Lrrc8c	T	C	5: 105,607,267 (GRCm38)	Y303H	probably damaging	Het
Mecr	T	G	4: 131,843,675 (GRCm38)	V58G	probably null	Het
Med12l	T	A	3: 59,042,403 (GRCm38)	D193E	possibly damaging	Het
Moap1	T	C	12: 102,743,179 (GRCm38)	E37G	probably damaging	Het
Mrgprb2	T	A	7: 48,552,896 (GRCm38)	Y27F	probably benign	Het
Ms4a6d	G	A	19: 11,590,073 (GRCm38)	Q155*	probably null	Het
Ndufs5	A	T	4: 123,715,951 (GRCm38)	C43*	probably null	Het
Oaz2	A	T	9: 65,688,901 (GRCm38)	D83V		Het
Olfr204	T	A	16: 59,315,149 (GRCm38)	N86I	possibly damaging	Het
Olfr329-ps	A	T	11: 58,542,707 (GRCm38)	Y269*	probably null	Het
Palm	A	G	10: 79,816,863 (GRCm38)	T191A	probably damaging	Het
Pclo	C	A	5: 14,675,836 (GRCm38)	D1569E	unknown	Het
Ppid	T	A	3: 79,600,297 (GRCm38)	D264E	probably benign	Het
Ppp1r3a	A	G	6: 14,718,750 (GRCm38)	S722P	probably damaging	Het
Ranbp2	A	G	10: 58,485,797 (GRCm38)	S2639G	possibly damaging	Het
Rbak	T	C	5: 143,176,072 (GRCm38)	T68A	probably benign	Het
Rbm15b	C	T	9: 106,885,047 (GRCm38)	G641R	probably benign	Het
Rgsl1	A	G	1: 153,793,845 (GRCm38)	I265T	probably benign	Het
Rmdn1	T	A	4: 19,586,837 (GRCm38)	Y98*	probably null	Het
Sfxn3	T	G	19: 45,049,262 (GRCm38)	N52K	probably benign	Het
Slc17a3	G	T	13: 23,846,884 (GRCm38)	G182*	probably null	Het
Slc29a3	A	T	10: 60,750,658 (GRCm38)	Y10N	probably benign	Het
Slc43a1	T	C	2: 84,862,934 (GRCm38)	Y577H	probably damaging	Het
Smarcd3	T	A	5: 24,595,437 (GRCm38)	I210F	possibly damaging	Het
Snapc4	A	T	2: 26,369,261 (GRCm38)	S626T	probably benign	Het
Sp5	T	A	2: 70,476,697 (GRCm38)	L242*	probably null	Het
Spata25	C	G	2: 164,827,662 (GRCm38)	G143A	probably damaging	Het
Spata6	T	A	4: 111,768,738 (GRCm38)	Y101*	probably null	Het
Tdp2	T	G	13: 24,832,076 (GRCm38)	S89A	probably benign	Het
Tlr6	T	C	5: 64,953,629 (GRCm38)	E645G	probably benign	Het
Tmem209	T	C	6: 30,494,795 (GRCm38)	I355V	probably benign	Het
Trappc8	A	T	18: 20,852,647 (GRCm38)	Y646N	probably damaging	Het
Tspoap1	A	G	11: 87,766,379 (GRCm38)	N399S	probably damaging	Het
Ube4b	A	G	4: 149,343,001 (GRCm38)	Y897H	probably damaging	Het
Urb1	CACTTAC	CAC	16: 90,772,573 (GRCm38)		probably benign	Het
Usp9y	A	T	Y: 1,315,759 (GRCm38)		probably null	Het
Vmn2r1	T	C	3: 64,105,456 (GRCm38)	*913Q	probably null	Het
Vmn2r12	C	T	5: 109,091,945 (GRCm38)	A251T	possibly damaging	Het
Vmn2r12	T	A	5: 109,086,247 (GRCm38)	T700S	possibly damaging	Het
Wdr70	A	C	15: 7,924,244 (GRCm38)	F389V	possibly damaging	Het
Wdr91	A	T	6: 34,891,460 (GRCm38)	M442K	possibly damaging	Het
Ydjc	T	C	16: 17,150,898 (GRCm38)	C234R	probably benign	Het
Zfp72	A	G	13: 74,372,348 (GRCm38)	Y204H	possibly damaging	Het

Other mutations in Frem2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00895:Frem2	APN	3	53,585,595 (GRCm38)	missense	probably damaging	1.00
IGL00911:Frem2	APN	3	53,572,462 (GRCm38)	missense	probably damaging	1.00
IGL01322:Frem2	APN	3	53,541,038 (GRCm38)	missense	probably benign	0.00
IGL01330:Frem2	APN	3	53,655,241 (GRCm38)	missense	possibly damaging	0.70
IGL01406:Frem2	APN	3	53,525,896 (GRCm38)	missense	probably damaging	1.00
IGL01556:Frem2	APN	3	53,535,281 (GRCm38)	missense	probably benign	0.23
IGL01580:Frem2	APN	3	53,655,175 (GRCm38)	missense	probably damaging	1.00
IGL01606:Frem2	APN	3	53,653,591 (GRCm38)	missense	possibly damaging	0.69
IGL01611:Frem2	APN	3	53,655,709 (GRCm38)	missense	probably benign	0.00
IGL01648:Frem2	APN	3	53,535,732 (GRCm38)	missense	possibly damaging	0.86
IGL01663:Frem2	APN	3	53,517,013 (GRCm38)	missense	probably damaging	1.00
IGL01665:Frem2	APN	3	53,549,662 (GRCm38)	missense	probably benign	0.07
IGL01670:Frem2	APN	3	53,656,937 (GRCm38)	missense	possibly damaging	0.95
IGL01960:Frem2	APN	3	53,522,304 (GRCm38)	missense	probably benign	0.33
IGL02175:Frem2	APN	3	53,655,599 (GRCm38)	missense	possibly damaging	0.69
IGL02201:Frem2	APN	3	53,519,640 (GRCm38)	missense	probably benign	0.35
IGL02202:Frem2	APN	3	53,654,799 (GRCm38)	missense	probably benign	0.00
IGL02427:Frem2	APN	3	53,535,763 (GRCm38)	missense	probably damaging	0.97
IGL02457:Frem2	APN	3	53,521,049 (GRCm38)	missense	probably damaging	0.99
IGL02638:Frem2	APN	3	53,551,346 (GRCm38)	missense	possibly damaging	0.94
IGL02801:Frem2	APN	3	53,652,175 (GRCm38)	missense	possibly damaging	0.85
IGL03023:Frem2	APN	3	53,655,628 (GRCm38)	missense	probably benign	0.40
IGL03169:Frem2	APN	3	53,522,292 (GRCm38)	missense	probably benign	0.01
IGL03238:Frem2	APN	3	53,656,261 (GRCm38)	missense	possibly damaging	0.93
IGL03251:Frem2	APN	3	53,572,308 (GRCm38)	missense	probably benign	0.01
IGL03273:Frem2	APN	3	53,537,509 (GRCm38)	nonsense	probably null
IGL03343:Frem2	APN	3	53,652,253 (GRCm38)	missense	probably damaging	1.00
Biosimilar	UTSW	3	53,654,323 (GRCm38)	missense	probably benign	0.01
Fruit_stripe	UTSW	3	53,537,489 (GRCm38)	missense	probably benign	0.21
PIT4366001:Frem2	UTSW	3	53,653,201 (GRCm38)	missense	probably damaging	0.98
R0019:Frem2	UTSW	3	53,523,678 (GRCm38)	missense	probably damaging	0.99
R0092:Frem2	UTSW	3	53,589,796 (GRCm38)	missense	probably benign	0.03
R0108:Frem2	UTSW	3	53,647,961 (GRCm38)	missense	probably benign	0.03
R0115:Frem2	UTSW	3	53,656,208 (GRCm38)	missense	probably damaging	0.99
R0118:Frem2	UTSW	3	53,535,243 (GRCm38)	nonsense	probably null
R0374:Frem2	UTSW	3	53,653,960 (GRCm38)	missense	probably damaging	1.00
R0437:Frem2	UTSW	3	53,653,015 (GRCm38)	missense	possibly damaging	0.96
R0531:Frem2	UTSW	3	53,519,954 (GRCm38)	missense	probably damaging	1.00
R0555:Frem2	UTSW	3	53,516,860 (GRCm38)	missense	probably damaging	0.97
R0564:Frem2	UTSW	3	53,656,109 (GRCm38)	missense	probably damaging	0.97
R0586:Frem2	UTSW	3	53,647,921 (GRCm38)	missense	probably damaging	0.99
R0726:Frem2	UTSW	3	53,519,626 (GRCm38)	missense	possibly damaging	0.89
R0925:Frem2	UTSW	3	53,653,973 (GRCm38)	missense	probably benign
R1233:Frem2	UTSW	3	53,547,778 (GRCm38)	missense	probably damaging	0.98
R1302:Frem2	UTSW	3	53,655,538 (GRCm38)	missense	probably benign	0.00
R1333:Frem2	UTSW	3	53,549,731 (GRCm38)	missense	probably benign	0.26
R1446:Frem2	UTSW	3	53,654,596 (GRCm38)	missense	probably benign	0.31
R1523:Frem2	UTSW	3	53,655,407 (GRCm38)	missense	possibly damaging	0.73
R1539:Frem2	UTSW	3	53,654,210 (GRCm38)	missense	probably benign	0.19
R1543:Frem2	UTSW	3	53,572,455 (GRCm38)	missense	possibly damaging	0.86
R1597:Frem2	UTSW	3	53,654,519 (GRCm38)	missense	probably benign	0.19
R1600:Frem2	UTSW	3	53,547,723 (GRCm38)	missense	probably damaging	1.00
R1678:Frem2	UTSW	3	53,519,938 (GRCm38)	missense	probably damaging	1.00
R1687:Frem2	UTSW	3	53,653,952 (GRCm38)	missense	probably benign
R1696:Frem2	UTSW	3	53,656,042 (GRCm38)	nonsense	probably null
R1758:Frem2	UTSW	3	53,653,357 (GRCm38)	missense	probably damaging	1.00
R1857:Frem2	UTSW	3	53,654,873 (GRCm38)	missense	probably benign	0.10
R1869:Frem2	UTSW	3	53,535,196 (GRCm38)	missense	probably benign	0.04
R1921:Frem2	UTSW	3	53,653,495 (GRCm38)	missense	possibly damaging	0.76
R1973:Frem2	UTSW	3	53,652,232 (GRCm38)	missense	probably benign	0.01
R2045:Frem2	UTSW	3	53,535,744 (GRCm38)	missense	probably damaging	1.00
R2113:Frem2	UTSW	3	53,652,922 (GRCm38)	missense	probably damaging	1.00
R2152:Frem2	UTSW	3	53,517,029 (GRCm38)	nonsense	probably null
R2164:Frem2	UTSW	3	53,537,330 (GRCm38)	missense	probably damaging	1.00
R2181:Frem2	UTSW	3	53,574,587 (GRCm38)	missense	possibly damaging	0.72
R2201:Frem2	UTSW	3	53,516,573 (GRCm38)	missense	probably benign
R2221:Frem2	UTSW	3	53,516,857 (GRCm38)	missense	probably benign	0.00
R2255:Frem2	UTSW	3	53,652,514 (GRCm38)	missense	probably damaging	0.96
R2280:Frem2	UTSW	3	53,572,423 (GRCm38)	missense	probably damaging	1.00
R3196:Frem2	UTSW	3	53,537,331 (GRCm38)	missense	probably damaging	1.00
R3716:Frem2	UTSW	3	53,572,360 (GRCm38)	missense	probably damaging	1.00
R3807:Frem2	UTSW	3	53,653,449 (GRCm38)	missense	probably benign	0.22
R3820:Frem2	UTSW	3	53,516,849 (GRCm38)	missense	probably damaging	1.00
R3821:Frem2	UTSW	3	53,652,415 (GRCm38)	missense	probably damaging	1.00
R3977:Frem2	UTSW	3	53,652,070 (GRCm38)	missense	probably benign	0.00
R3979:Frem2	UTSW	3	53,652,070 (GRCm38)	missense	probably benign	0.00
R4014:Frem2	UTSW	3	53,652,353 (GRCm38)	missense	probably benign	0.01
R4127:Frem2	UTSW	3	53,525,896 (GRCm38)	missense	probably damaging	1.00
R4195:Frem2	UTSW	3	53,539,268 (GRCm38)	missense	possibly damaging	0.90
R4196:Frem2	UTSW	3	53,539,268 (GRCm38)	missense	possibly damaging	0.90
R4374:Frem2	UTSW	3	53,545,502 (GRCm38)	missense	possibly damaging	0.61
R4427:Frem2	UTSW	3	53,539,162 (GRCm38)	critical splice donor site	probably null
R4428:Frem2	UTSW	3	53,654,338 (GRCm38)	missense	probably benign	0.40
R4559:Frem2	UTSW	3	53,654,321 (GRCm38)	missense	probably benign	0.01
R4600:Frem2	UTSW	3	53,547,807 (GRCm38)	missense	possibly damaging	0.96
R4602:Frem2	UTSW	3	53,547,807 (GRCm38)	missense	possibly damaging	0.96
R4610:Frem2	UTSW	3	53,547,807 (GRCm38)	missense	possibly damaging	0.96
R4611:Frem2	UTSW	3	53,547,807 (GRCm38)	missense	possibly damaging	0.96
R4661:Frem2	UTSW	3	53,655,443 (GRCm38)	missense	probably damaging	1.00
R4678:Frem2	UTSW	3	53,544,371 (GRCm38)	missense	probably benign	0.00
R4689:Frem2	UTSW	3	53,547,635 (GRCm38)	missense	probably benign	0.43
R4740:Frem2	UTSW	3	53,535,819 (GRCm38)	missense	probably benign	0.04
R4748:Frem2	UTSW	3	53,541,093 (GRCm38)	missense	probably damaging	1.00
R4790:Frem2	UTSW	3	53,516,741 (GRCm38)	missense	probably benign
R4809:Frem2	UTSW	3	53,653,895 (GRCm38)	missense	probably benign	0.01
R4930:Frem2	UTSW	3	53,656,315 (GRCm38)	missense	possibly damaging	0.93
R4971:Frem2	UTSW	3	53,539,183 (GRCm38)	missense	probably damaging	1.00
R5057:Frem2	UTSW	3	53,535,196 (GRCm38)	missense	probably benign	0.37
R5202:Frem2	UTSW	3	53,551,346 (GRCm38)	missense	probably benign	0.41
R5221:Frem2	UTSW	3	53,585,611 (GRCm38)	missense	probably damaging	1.00
R5231:Frem2	UTSW	3	53,522,295 (GRCm38)	missense	probably damaging	1.00
R5268:Frem2	UTSW	3	53,653,154 (GRCm38)	missense	probably damaging	0.96
R5480:Frem2	UTSW	3	53,656,507 (GRCm38)	nonsense	probably null
R5637:Frem2	UTSW	3	53,652,937 (GRCm38)	missense	probably damaging	0.97
R5664:Frem2	UTSW	3	53,652,490 (GRCm38)	missense	probably benign	0.33
R5698:Frem2	UTSW	3	53,652,505 (GRCm38)	missense	possibly damaging	0.89
R5744:Frem2	UTSW	3	53,655,959 (GRCm38)	missense	probably damaging	1.00
R5754:Frem2	UTSW	3	53,537,258 (GRCm38)	missense	probably damaging	1.00
R5808:Frem2	UTSW	3	53,652,563 (GRCm38)	missense	probably damaging	0.96
R5840:Frem2	UTSW	3	53,647,921 (GRCm38)	missense	probably damaging	0.99
R5874:Frem2	UTSW	3	53,537,489 (GRCm38)	missense	probably benign	0.21
R6050:Frem2	UTSW	3	53,653,012 (GRCm38)	missense	probably damaging	0.99
R6103:Frem2	UTSW	3	53,549,788 (GRCm38)	missense	probably benign	0.00
R6149:Frem2	UTSW	3	53,551,341 (GRCm38)	missense	probably damaging	0.98
R6182:Frem2	UTSW	3	53,647,969 (GRCm38)	missense	probably damaging	1.00
R6191:Frem2	UTSW	3	53,655,280 (GRCm38)	missense	probably benign	0.10
R6245:Frem2	UTSW	3	53,655,824 (GRCm38)	missense	probably benign	0.00
R6252:Frem2	UTSW	3	53,572,448 (GRCm38)	missense	probably damaging	1.00
R6393:Frem2	UTSW	3	53,585,640 (GRCm38)	missense	possibly damaging	0.91
R6416:Frem2	UTSW	3	53,572,378 (GRCm38)	missense	probably benign	0.01
R6595:Frem2	UTSW	3	53,549,784 (GRCm38)	missense	probably damaging	1.00
R6665:Frem2	UTSW	3	53,654,656 (GRCm38)	missense	probably damaging	1.00
R6708:Frem2	UTSW	3	53,585,501 (GRCm38)	missense	probably benign	0.00
R6751:Frem2	UTSW	3	53,653,665 (GRCm38)	missense	probably damaging	1.00
R6787:Frem2	UTSW	3	53,654,323 (GRCm38)	missense	probably benign	0.01
R6913:Frem2	UTSW	3	53,516,821 (GRCm38)	missense	probably damaging	1.00
R6916:Frem2	UTSW	3	53,547,688 (GRCm38)	missense	probably damaging	1.00
R7017:Frem2	UTSW	3	53,519,602 (GRCm38)	missense	probably benign	0.02
R7083:Frem2	UTSW	3	53,537,493 (GRCm38)	missense	probably damaging	0.99
R7108:Frem2	UTSW	3	53,653,513 (GRCm38)	missense	probably damaging	1.00
R7133:Frem2	UTSW	3	53,572,339 (GRCm38)	missense	possibly damaging	0.82
R7326:Frem2	UTSW	3	53,654,753 (GRCm38)	missense	probably damaging	1.00
R7455:Frem2	UTSW	3	53,572,280 (GRCm38)	splice site	probably null
R7487:Frem2	UTSW	3	53,654,549 (GRCm38)	missense	probably benign	0.40
R7495:Frem2	UTSW	3	53,516,837 (GRCm38)	missense	probably benign	0.13
R7542:Frem2	UTSW	3	53,652,579 (GRCm38)	missense	probably damaging	1.00
R7636:Frem2	UTSW	3	53,653,247 (GRCm38)	missense	probably benign	0.00
R7703:Frem2	UTSW	3	53,522,168 (GRCm38)	missense	probably benign	0.01
R7750:Frem2	UTSW	3	53,523,682 (GRCm38)	missense	possibly damaging	0.83
R7849:Frem2	UTSW	3	53,572,374 (GRCm38)	missense	probably damaging	1.00
R7922:Frem2	UTSW	3	53,653,304 (GRCm38)	missense	probably damaging	0.98
R8008:Frem2	UTSW	3	53,652,910 (GRCm38)	missense	probably damaging	1.00
R8051:Frem2	UTSW	3	53,535,355 (GRCm38)	missense	probably benign	0.04
R8052:Frem2	UTSW	3	53,549,643 (GRCm38)	missense	probably benign	0.02
R8176:Frem2	UTSW	3	53,655,340 (GRCm38)	missense	possibly damaging	0.50
R8220:Frem2	UTSW	3	53,656,507 (GRCm38)	nonsense	probably null
R8397:Frem2	UTSW	3	53,653,141 (GRCm38)	missense	probably benign	0.00
R8410:Frem2	UTSW	3	53,539,177 (GRCm38)	missense	possibly damaging	0.60
R8697:Frem2	UTSW	3	53,525,828 (GRCm38)	missense	probably damaging	0.99
R9134:Frem2	UTSW	3	53,654,900 (GRCm38)	missense	probably damaging	1.00
R9183:Frem2	UTSW	3	53,520,065 (GRCm38)	missense	probably damaging	1.00
R9260:Frem2	UTSW	3	53,652,783 (GRCm38)	missense	probably damaging	1.00
R9267:Frem2	UTSW	3	53,657,083 (GRCm38)	start codon destroyed	probably null	0.00
R9299:Frem2	UTSW	3	53,656,559 (GRCm38)	missense	probably benign	0.37
R9378:Frem2	UTSW	3	53,651,989 (GRCm38)	missense	probably damaging	0.99
R9444:Frem2	UTSW	3	53,652,844 (GRCm38)	missense	probably benign	0.10
R9459:Frem2	UTSW	3	53,653,486 (GRCm38)	missense	probably benign
R9487:Frem2	UTSW	3	53,653,484 (GRCm38)	missense	possibly damaging	0.95
R9728:Frem2	UTSW	3	53,656,631 (GRCm38)	missense	probably benign	0.00
R9759:Frem2	UTSW	3	53,655,497 (GRCm38)	missense	possibly damaging	0.76
Z1177:Frem2	UTSW	3	53,655,607 (GRCm38)	missense	probably benign	0.31
Z1177:Frem2	UTSW	3	53,535,166 (GRCm38)	missense	probably null	1.00

Predicted Primers

PCR Primer
(F):5'- CCGAACGTTCACTCTGAGAG -3'
(R):5'- TGGCTCAGTTCCAGTTCCAAG -3'

Sequencing Primer
(F):5'- ATCCCCGCTATTCCAGTA -3'
(R):5'- CTCAGTTCCAGTTCCAAGTAGAGG -3'

Posted On

2019-09-13