Mutagenetix > Incidental Mutations

Incidental Mutation 'R7345:Dcaf10'

570130

Institutional Source

Beutler Lab

Gene Symbol

Dcaf10

Ensembl Gene

ENSMUSG00000035572

Gene Name

DDB1 and CUL4 associated factor 10

Synonyms

Wdr32

MMRRC Submission

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7345 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

45342101-45379759 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 45342583 bp

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 139 (L139P)

Ref Sequence

ENSEMBL: ENSMUSP00000117082 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000155551]

Predicted Effect

probably benign
Transcript: ENSMUST00000152056

SMART Domains

Protein: ENSMUSP00000115532
Gene: ENSMUSG00000028322

Domain	Start	End	E-Value	Type
Blast:S1	24	93	8e-38	BLAST
Pfam:KH_6	109	145	8e-13	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000155551
AA Change: L139P

PolyPhen 2 Score 0.977 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000117082
Gene: ENSMUSG00000035572
AA Change: L139P

Domain	Start	End	E-Value	Type
low complexity region	15	46	N/A	INTRINSIC
low complexity region	80	107	N/A	INTRINSIC
low complexity region	110	132	N/A	INTRINSIC
WD40	166	203	1.71e1	SMART
WD40	206	245	7.85e-7	SMART
WD40	249	288	2.59e-7	SMART
WD40	295	334	2.05e1	SMART
low complexity region	352	374	N/A	INTRINSIC
Blast:WD40	468	506	3e-10	BLAST
WD40	524	563	1.31e-3	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 98.9%

Validation Efficiency

100% (55/55)

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Anks3	A	G	16: 4,955,910	M174T	possibly damaging	Het
Arhgap23	G	A	11: 97,466,478	R934Q	possibly damaging	Het
Atad5	T	C	11: 80,096,006	S640P	probably damaging	Het
B3galnt2	C	T	13: 13,980,480		probably null	Het
B430305J03Rik	T	C	3: 61,364,118	S69G	unknown	Het
C87499	T	C	4: 88,628,179	K309E	possibly damaging	Het
Cd163	A	T	6: 124,318,938	N747I	possibly damaging	Het
Cep126	A	G	9: 8,099,816	S906P	probably damaging	Het
Cyp4f39	G	A	17: 32,486,779	G318R	probably damaging	Het
D630003M21Rik	C	T	2: 158,217,209	G257D	probably damaging	Het
Dcc	A	G	18: 71,378,824	V840A	probably benign	Het
Dnhd1	A	T	7: 105,703,967	I2776L	probably benign	Het
Fam166b	T	C	4: 43,428,022	D145G	possibly damaging	Het
Fbxo24	G	T	5: 137,621,261	F234L	probably damaging	Het
Gm21190	T	C	5: 15,527,904		probably null	Het
Grhl3	C	T	4: 135,546,246	R565Q	probably damaging	Het
Gtf3c1	A	T	7: 125,645,670	Y1731N	probably damaging	Het
Il1a	C	A	2: 129,304,773	R133S	probably benign	Het
Ipo4	C	T	14: 55,635,531	R23Q	probably benign	Het
Klc3	G	A	7: 19,394,889	T481M	probably benign	Het
Map2k6	A	T	11: 110,492,908	I127F		Het
Mapk8	A	T	14: 33,408,111	N63K	probably damaging	Het
Med13l	T	C	5: 118,742,760	W1306R	probably damaging	Het
Megf6	C	A	4: 154,267,315	Q1162K	probably benign	Het
Myo5b	A	G	18: 74,708,024	E992G	possibly damaging	Het
Ndst4	C	T	3: 125,714,659	T291M	probably benign	Het
Nek10	T	A	14: 14,955,503	F838L	probably benign	Het
Nrm	A	G	17: 35,864,584	H194R	probably damaging	Het
Nup188	T	C	2: 30,340,601	S1384P	probably benign	Het
Olfml2a	C	A	2: 38,960,127	D618E	probably damaging	Het
Olfr466	A	C	13: 65,152,743	N173T	possibly damaging	Het
Olfr506	C	T	7: 108,613,063	T252I	probably benign	Het
Olfr937	A	G	9: 39,060,579	L29P	probably damaging	Het
Olfr938	G	A	9: 39,078,334	S137F	probably damaging	Het
P2ry1	T	C	3: 61,003,674	F78S	possibly damaging	Het
Pcdhb18	T	C	18: 37,491,923	F769L	probably benign	Het
Poc5	A	G	13: 96,396,796	E144G	probably damaging	Het
Pole	T	A	5: 110,303,903	N870K	possibly damaging	Het
Polr2b	T	C	5: 77,349,119	F1159L	possibly damaging	Het
Rubcnl	A	G	14: 75,042,353	Y392C	probably benign	Het
Sec31a	T	C	5: 100,385,270	T539A	probably damaging	Het
Serpinh1	A	C	7: 99,346,356	S340A	probably damaging	Het
Slc1a5	T	A	7: 16,796,160		probably null	Het
Soat2	A	G	15: 102,162,578	D469G	probably benign	Het
Speg	T	C	1: 75,384,835	L70P	probably damaging	Het
Spred2	T	C	11: 19,924,958		probably null	Het
Stac2	A	C	11: 98,042,613	S168A	probably damaging	Het
Stx1a	T	A	5: 135,037,188	D31E	probably benign	Het
Timeless	C	T	10: 128,249,754	T885M	probably damaging	Het
Tmem94	G	T	11: 115,786,256	R118L	possibly damaging	Het
Tsen34	T	C	7: 3,695,615	Y253H	probably damaging	Het
Unc13b	T	C	4: 43,173,966	V1598A	unknown	Het
Zfat	A	T	15: 68,105,043	Y1086N	probably damaging	Het
Zfp551	G	A	7: 12,416,595	H296Y	probably benign	Het

Other mutations in Dcaf10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02431:Dcaf10	APN	4	45342630	missense	probably benign	0.01
IGL02660:Dcaf10	APN	4	45372769	missense	possibly damaging	0.92
R0048:Dcaf10	UTSW	4	45374262	nonsense	probably null
R0550:Dcaf10	UTSW	4	45372753	missense	probably benign
R0611:Dcaf10	UTSW	4	45373011	missense	probably damaging	1.00
R2289:Dcaf10	UTSW	4	45359816	missense	probably damaging	1.00
R2973:Dcaf10	UTSW	4	45373957	missense	probably benign	0.04
R3610:Dcaf10	UTSW	4	45372962	nonsense	probably null
R3735:Dcaf10	UTSW	4	45348117	missense	probably benign	0.01
R4655:Dcaf10	UTSW	4	45372769	missense	possibly damaging	0.92
R4665:Dcaf10	UTSW	4	45372769	missense	possibly damaging	0.92
R4690:Dcaf10	UTSW	4	45372769	missense	possibly damaging	0.92
R4724:Dcaf10	UTSW	4	45372769	missense	possibly damaging	0.92
R4725:Dcaf10	UTSW	4	45372769	missense	possibly damaging	0.92
R4735:Dcaf10	UTSW	4	45372769	missense	possibly damaging	0.92
R4743:Dcaf10	UTSW	4	45370409	missense	probably damaging	0.98
R5220:Dcaf10	UTSW	4	45373909	missense	possibly damaging	0.94
R5254:Dcaf10	UTSW	4	45370415	missense	possibly damaging	0.94
R5855:Dcaf10	UTSW	4	45342558	missense	probably benign	0.18
R6833:Dcaf10	UTSW	4	45373043	missense	probably damaging	1.00
R7132:Dcaf10	UTSW	4	45342391	missense	probably benign
R7366:Dcaf10	UTSW	4	45373919	missense	probably damaging	1.00
R7832:Dcaf10	UTSW	4	45348196	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGTCATGTTTCCCTTCGGGC -3'
(R):5'- CTCATAGGTGACAAAGTACCGG -3'

Sequencing Primer
(F):5'- CGGAGCTCTGGAGTTGTCC -3'
(R):5'- CAGCCTGAGCGGAGGGAG -3'

Posted On

2019-09-13