Incidental Mutation 'R7345:Spred2'
ID 570153
Institutional Source Beutler Lab
Gene Symbol Spred2
Ensembl Gene ENSMUSG00000045671
Gene Name sprouty-related, EVH1 domain containing 2
Synonyms Spred-2
MMRRC Submission
Accession Numbers
Is this an essential gene? Possibly non essential (E-score: 0.387) question?
Stock # R7345 (G1)
Quality Score 225.009
Status Validated
Chromosome 11
Chromosomal Location 19924375-20024026 bp(+) (GRCm38)
Type of Mutation critical splice donor site (2 bp from exon)
DNA Base Change (assembly) T to C at 19924958 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000090987 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000093298] [ENSMUST00000093299]
AlphaFold Q924S7
Predicted Effect probably null
Transcript: ENSMUST00000093298
SMART Domains Protein: ENSMUSP00000090987
Gene: ENSMUSG00000045671

DomainStartEndE-ValueType
Pfam:WH1 6 119 4.7e-13 PFAM
Pfam:Sprouty 298 403 3.8e-44 PFAM
Predicted Effect probably null
Transcript: ENSMUST00000093299
SMART Domains Protein: ENSMUSP00000090988
Gene: ENSMUSG00000045671

DomainStartEndE-ValueType
Pfam:WH1 91 146 4.1e-10 PFAM
Pfam:Sprouty 325 430 8.6e-29 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.7%
  • 20x: 98.9%
Validation Efficiency 100% (55/55)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM, Mar 2008]
PHENOTYPE: Homozygous null mice are fertile and display increased hematopoietic cell formation in culture. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 54 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Anks3 A G 16: 4,955,910 M174T possibly damaging Het
Arhgap23 G A 11: 97,466,478 R934Q possibly damaging Het
Atad5 T C 11: 80,096,006 S640P probably damaging Het
B3galnt2 C T 13: 13,980,480 probably null Het
B430305J03Rik T C 3: 61,364,118 S69G unknown Het
C87499 T C 4: 88,628,179 K309E possibly damaging Het
Cd163 A T 6: 124,318,938 N747I possibly damaging Het
Cep126 A G 9: 8,099,816 S906P probably damaging Het
Cyp4f39 G A 17: 32,486,779 G318R probably damaging Het
D630003M21Rik C T 2: 158,217,209 G257D probably damaging Het
Dcaf10 T C 4: 45,342,583 L139P probably damaging Het
Dcc A G 18: 71,378,824 V840A probably benign Het
Dnhd1 A T 7: 105,703,967 I2776L probably benign Het
Fam166b T C 4: 43,428,022 D145G possibly damaging Het
Fbxo24 G T 5: 137,621,261 F234L probably damaging Het
Gm21190 T C 5: 15,527,904 probably null Het
Grhl3 C T 4: 135,546,246 R565Q probably damaging Het
Gtf3c1 A T 7: 125,645,670 Y1731N probably damaging Het
Il1a C A 2: 129,304,773 R133S probably benign Het
Ipo4 C T 14: 55,635,531 R23Q probably benign Het
Klc3 G A 7: 19,394,889 T481M probably benign Het
Map2k6 A T 11: 110,492,908 I127F Het
Mapk8 A T 14: 33,408,111 N63K probably damaging Het
Med13l T C 5: 118,742,760 W1306R probably damaging Het
Megf6 C A 4: 154,267,315 Q1162K probably benign Het
Myo5b A G 18: 74,708,024 E992G possibly damaging Het
Ndst4 C T 3: 125,714,659 T291M probably benign Het
Nek10 T A 14: 14,955,503 F838L probably benign Het
Nrm A G 17: 35,864,584 H194R probably damaging Het
Nup188 T C 2: 30,340,601 S1384P probably benign Het
Olfml2a C A 2: 38,960,127 D618E probably damaging Het
Olfr466 A C 13: 65,152,743 N173T possibly damaging Het
Olfr506 C T 7: 108,613,063 T252I probably benign Het
Olfr937 A G 9: 39,060,579 L29P probably damaging Het
Olfr938 G A 9: 39,078,334 S137F probably damaging Het
P2ry1 T C 3: 61,003,674 F78S possibly damaging Het
Pcdhb18 T C 18: 37,491,923 F769L probably benign Het
Poc5 A G 13: 96,396,796 E144G probably damaging Het
Pole T A 5: 110,303,903 N870K possibly damaging Het
Polr2b T C 5: 77,349,119 F1159L possibly damaging Het
Rubcnl A G 14: 75,042,353 Y392C probably benign Het
Sec31a T C 5: 100,385,270 T539A probably damaging Het
Serpinh1 A C 7: 99,346,356 S340A probably damaging Het
Slc1a5 T A 7: 16,796,160 probably null Het
Soat2 A G 15: 102,162,578 D469G probably benign Het
Speg T C 1: 75,384,835 L70P probably damaging Het
Stac2 A C 11: 98,042,613 S168A probably damaging Het
Stx1a T A 5: 135,037,188 D31E probably benign Het
Timeless C T 10: 128,249,754 T885M probably damaging Het
Tmem94 G T 11: 115,786,256 R118L possibly damaging Het
Tsen34 T C 7: 3,695,615 Y253H probably damaging Het
Unc13b T C 4: 43,173,966 V1598A unknown Het
Zfat A T 15: 68,105,043 Y1086N probably damaging Het
Zfp551 G A 7: 12,416,595 H296Y probably benign Het
Other mutations in Spred2
AlleleSourceChrCoordTypePredicted EffectPPH Score
R0512:Spred2 UTSW 11 20008485 splice site probably benign
R0944:Spred2 UTSW 11 20001104 splice site probably benign
R1467:Spred2 UTSW 11 20018109 missense probably benign 0.00
R1467:Spred2 UTSW 11 20018109 missense probably benign 0.00
R2156:Spred2 UTSW 11 20021241 missense probably damaging 1.00
R2915:Spred2 UTSW 11 19998215 missense probably damaging 1.00
R3433:Spred2 UTSW 11 19998277 nonsense probably null
R4839:Spred2 UTSW 11 19998233 missense possibly damaging 0.53
R5100:Spred2 UTSW 11 20021291 nonsense probably null
R5353:Spred2 UTSW 11 20018155 missense possibly damaging 0.82
R5529:Spred2 UTSW 11 20021301 missense probably damaging 1.00
R5709:Spred2 UTSW 11 20021415 missense probably damaging 1.00
R6978:Spred2 UTSW 11 19998254 missense possibly damaging 0.73
R8073:Spred2 UTSW 11 20008422 missense probably benign 0.45
R8847:Spred2 UTSW 11 20001064 missense probably benign 0.03
R8888:Spred2 UTSW 11 20001019 missense probably benign 0.11
R8895:Spred2 UTSW 11 20001019 missense probably benign 0.11
X0025:Spred2 UTSW 11 19998234 missense probably benign 0.01
Predicted Primers PCR Primer
(F):5'- ACTACCCGGAACCCTGATTG -3'
(R):5'- GAGTCACAAAGTCACCATGC -3'

Sequencing Primer
(F):5'- AGACCTCGCCCTGATCTCG -3'
(R):5'- TGCGACCTGGACTTGCAGAC -3'
Posted On 2019-09-13