Mutagenetix > Incidental Mutations

Incidental Mutation 'R7346:Ifi44'

570187

Institutional Source

Beutler Lab

Gene Symbol

Ifi44

Ensembl Gene

ENSMUSG00000028037

Gene Name

interferon-induced protein 44

Synonyms

A430056A10Rik, p44, MTAP44

MMRRC Submission

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7346 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

151730922-151749960 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 151732457 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Leucine at position 398 (M398L)

Ref Sequence

ENSEMBL: ENSMUSP00000029671 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000029671]

AlphaFold

Q8BV66

Predicted Effect

probably benign
Transcript: ENSMUST00000029671
AA Change: M398L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000029671
Gene: ENSMUSG00000028037
AA Change: M398L

Domain	Start	End	E-Value	Type
Pfam:TLD	26	147	2.8e-7	PFAM
low complexity region	193	208	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.1%

Validation Efficiency

100% (71/71)

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930563M21Rik	G	A	9: 56,007,303	P52S	unknown	Het
Adarb2	A	G	13: 8,570,384	E302G	probably damaging	Het
Adgrf5	T	A	17: 43,451,179	L1255H	probably damaging	Het
Adra1a	T	A	14: 66,638,284	I236N	probably benign	Het
As3mt	T	C	19: 46,720,452	F295S	probably damaging	Het
Bmp5	G	A	9: 75,873,360	R313Q	probably damaging	Het
Ccnj	T	A	19: 40,844,950	S191T	probably benign	Het
Clk2	T	A	3: 89,173,545		probably null	Het
Cpz	T	C	5: 35,517,656	E83G	probably damaging	Het
Cts3	A	G	13: 61,567,620	I133T	probably benign	Het
Dars2	C	T	1: 161,046,772		probably null	Het
Dsel	A	T	1: 111,861,068	V579D	probably damaging	Het
Dync1h1	C	A	12: 110,635,642	A2038E	probably damaging	Het
Eaf1	A	G	14: 31,494,820		probably benign	Het
Fsip2	A	C	2: 82,998,180	I6774L	probably benign	Het
Glis2	T	C	16: 4,613,568	F320L	possibly damaging	Het
Hcar1	A	G	5: 123,879,630		probably benign	Het
Hectd1	A	T	12: 51,750,321	H2211Q	probably benign	Het
Hmcn1	A	G	1: 150,683,745	I2385T	probably damaging	Het
Il16	C	T	7: 83,644,041	E1273K	probably damaging	Het
Inpp5j	G	A	11: 3,501,065	T528I	probably damaging	Het
Kbtbd6	C	T	14: 79,453,187	P441S	probably damaging	Het
Kcnt1	C	T	2: 25,863,843		probably benign	Het
Kif26b	T	C	1: 178,530,741	L139P	probably damaging	Het
Klb	C	T	5: 65,348,631	Q74*	probably null	Het
L3mbtl1	G	A	2: 162,967,006	V600I	probably benign	Het
Lcp1	C	T	14: 75,210,506	A317V	possibly damaging	Het
Lipc	C	T	9: 70,812,747	G326D	probably damaging	Het
Mbnl2	T	A	14: 120,379,282	F103I	probably benign	Het
Mdm1	G	T	10: 118,164,288	E609*	probably null	Het
Med16	A	G	10: 79,908,816		probably null	Het
Mga	T	C	2: 119,935,527	V1424A	possibly damaging	Het
Mocs2	G	T	13: 114,828,174		probably null	Het
Morc1	C	T	16: 48,630,900		probably null	Het
Mpc2	T	A	1: 165,479,511	W94R	probably damaging	Het
Mrm3	G	A	11: 76,250,176	V337I	possibly damaging	Het
N4bp3	T	C	11: 51,645,606	H133R	probably benign	Het
Ncam2	G	T	16: 81,623,368	G810V	probably damaging	Het
Nipbl	T	C	15: 8,343,606	E1052G	possibly damaging	Het
Nlrp12	C	T	7: 3,249,257	V95M	probably damaging	Het
Nrip1	T	C	16: 76,293,356	N438D	possibly damaging	Het
Nsun4	C	A	4: 116,051,838	M508I	probably benign	Het
Pla2g2c	T	C	4: 138,734,339	S40P	probably damaging	Het
Plec	A	G	15: 76,197,978		probably null	Het
Ppfia2	G	T	10: 106,857,495	M620I	possibly damaging	Het
Ppp6c	A	G	2: 39,226,217	Y9H	probably damaging	Het
Prkd1	A	T	12: 50,648,834	I47N	possibly damaging	Het
Prr14l	T	C	5: 32,830,684	D489G	probably benign	Het
Ptch2	T	A	4: 117,114,652	Y1124N	probably benign	Het
Ptpra	T	G	2: 130,553,400	Y808D	probably damaging	Het
Rint1	T	A	5: 23,815,653	F558Y	possibly damaging	Het
Rnf215	T	A	11: 4,139,792	C268*	probably null	Het
Slc25a13	T	C	6: 6,181,100	E28G	possibly damaging	Het
Slc38a8	G	T	8: 119,499,815	Y78*	probably null	Het
Slc4a7	T	C	14: 14,775,000	M810T	probably damaging	Het
Slitrk1	A	G	14: 108,913,159	V40A	possibly damaging	Het
Sobp	A	T	10: 43,022,835	S251R	probably damaging	Het
Spata31d1a	G	A	13: 59,703,201	S371L	probably benign	Het
Tada2b	T	C	5: 36,476,836	T133A	possibly damaging	Het
Tbc1d20	A	G	2: 152,304,961	D90G	probably benign	Het
Tek	T	A	4: 94,827,296	I408K	probably benign	Het
Tgfb2	A	G	1: 186,649,880	F150S	probably benign	Het
Tmem63b	T	C	17: 45,666,591	T370A	probably benign	Het
Tmem94	G	T	11: 115,786,256	R118L	possibly damaging	Het
Tspan17	C	G	13: 54,792,621	L34V	probably benign	Het
Vmn1r238	A	T	18: 3,123,151	Y88N	probably damaging	Het
Vmn1r85	A	G	7: 13,085,037	I60T	probably damaging	Het
Vmn2r96	T	C	17: 18,582,767	M313T	probably benign	Het
Zp3r	G	A	1: 130,583,480	S387F	probably benign	Het

Other mutations in Ifi44

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00858:Ifi44	APN	3	151749580	missense	probably benign	0.03
IGL01477:Ifi44	APN	3	151745998	splice site	probably benign
IGL01571:Ifi44	APN	3	151745537	missense	probably damaging	0.97
IGL02165:Ifi44	APN	3	151749430	missense	probably damaging	1.00
IGL02238:Ifi44	APN	3	151732382	makesense	probably null
IGL02609:Ifi44	APN	3	151732497	missense	probably damaging	1.00
IGL02650:Ifi44	APN	3	151745855	missense	probably damaging	1.00
IGL02726:Ifi44	APN	3	151749596	start gained	probably benign
IGL02977:Ifi44	APN	3	151739379	missense	probably benign	0.00
R0201:Ifi44	UTSW	3	151745636	missense	probably damaging	1.00
R0454:Ifi44	UTSW	3	151745497	missense	possibly damaging	0.78
R0763:Ifi44	UTSW	3	151749498	missense	probably damaging	1.00
R1640:Ifi44	UTSW	3	151732534	missense	probably benign	0.00
R1747:Ifi44	UTSW	3	151749285	missense	probably benign	0.00
R2278:Ifi44	UTSW	3	151732388	missense	probably benign
R3816:Ifi44	UTSW	3	151749257	missense	possibly damaging	0.92
R4075:Ifi44	UTSW	3	151745976	missense	probably benign	0.00
R4084:Ifi44	UTSW	3	151745489	critical splice donor site	probably null
R4782:Ifi44	UTSW	3	151745592	missense	probably damaging	0.99
R5071:Ifi44	UTSW	3	151749632	start gained	probably benign
R5074:Ifi44	UTSW	3	151749632	start gained	probably benign
R6030:Ifi44	UTSW	3	151749558	missense	probably benign	0.00
R6030:Ifi44	UTSW	3	151749558	missense	probably benign	0.00
R6128:Ifi44	UTSW	3	151749186	missense	probably benign	0.00
R6192:Ifi44	UTSW	3	151745639	critical splice acceptor site	probably null
R6434:Ifi44	UTSW	3	151749189	missense	probably benign	0.02
R6536:Ifi44	UTSW	3	151732489	missense	probably benign	0.03
R6902:Ifi44	UTSW	3	151745899	missense	possibly damaging	0.92
R6946:Ifi44	UTSW	3	151745899	missense	possibly damaging	0.92
R7608:Ifi44	UTSW	3	151732408	missense	probably damaging	0.97
R7704:Ifi44	UTSW	3	151732424	missense	probably benign	0.44
R7971:Ifi44	UTSW	3	151749220	missense	possibly damaging	0.89
R8255:Ifi44	UTSW	3	151745982	missense	probably benign	0.00
R8428:Ifi44	UTSW	3	151739341	nonsense	probably null
R8940:Ifi44	UTSW	3	151749309	missense	probably benign	0.00
R9087:Ifi44	UTSW	3	151745880	missense	probably damaging	1.00
R9259:Ifi44	UTSW	3	151749238	missense	possibly damaging	0.75
R9436:Ifi44	UTSW	3	151749249	missense	probably benign	0.00
Z1176:Ifi44	UTSW	3	151732453	missense	probably damaging	1.00
Z1177:Ifi44	UTSW	3	151749438	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGGGCTACTCACATGCCAAC -3'
(R):5'- TGATCACCAAGCTCCTAGTTC -3'

Sequencing Primer
(F):5'- CAGGCTAAAGAAACAGACCTAGCAG -3'
(R):5'- CAAAGATTCTCGCTGAACTTGGAGC -3'

Posted On

2019-09-13