Mutagenetix > Incidental Mutation

Incidental Mutation 'R7350:Spen'

570488

Institutional Source

Beutler Lab

Gene Symbol

Spen

Ensembl Gene

ENSMUSG00000040761

Gene Name

spen family transcription repressor

Synonyms

Mint

MMRRC Submission

045436-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7350 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

141467890-141538597 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 141479385 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Lysine at position 644 (E644K)

Ref Sequence

ENSEMBL: ENSMUSP00000101412 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000078886] [ENSMUST00000105786]

AlphaFold

no structure available at present

Predicted Effect

unknown
Transcript: ENSMUST00000078886
AA Change: E621K

SMART Domains

Protein: ENSMUSP00000077925
Gene: ENSMUSG00000040761
AA Change: E621K

Domain	Start	End	E-Value	Type
RRM	7	77	7.77e-12	SMART
low complexity region	109	121	N/A	INTRINSIC
low complexity region	235	257	N/A	INTRINSIC
low complexity region	262	311	N/A	INTRINSIC
RRM	338	411	8.6e-5	SMART
RRM	441	511	1.56e-16	SMART
RRM	520	587	1.84e-13	SMART
low complexity region	617	632	N/A	INTRINSIC
low complexity region	669	691	N/A	INTRINSIC
low complexity region	695	720	N/A	INTRINSIC
low complexity region	749	773	N/A	INTRINSIC
coiled coil region	800	825	N/A	INTRINSIC
low complexity region	830	841	N/A	INTRINSIC
internal_repeat_2	844	954	6.27e-5	PROSPERO
coiled coil region	1494	1522	N/A	INTRINSIC
low complexity region	1587	1627	N/A	INTRINSIC
low complexity region	1635	1641	N/A	INTRINSIC
low complexity region	1642	1671	N/A	INTRINSIC
low complexity region	1747	1758	N/A	INTRINSIC
low complexity region	1810	1823	N/A	INTRINSIC
low complexity region	1888	1903	N/A	INTRINSIC
low complexity region	1940	1955	N/A	INTRINSIC
low complexity region	2003	2012	N/A	INTRINSIC
internal_repeat_2	2015	2115	6.27e-5	PROSPERO
low complexity region	2127	2147	N/A	INTRINSIC
low complexity region	2169	2191	N/A	INTRINSIC
low complexity region	2207	2219	N/A	INTRINSIC
low complexity region	2304	2323	N/A	INTRINSIC
low complexity region	2332	2371	N/A	INTRINSIC
low complexity region	2396	2413	N/A	INTRINSIC
low complexity region	2518	2533	N/A	INTRINSIC
low complexity region	2545	2555	N/A	INTRINSIC
low complexity region	2696	2722	N/A	INTRINSIC
low complexity region	2931	2942	N/A	INTRINSIC
low complexity region	2994	3006	N/A	INTRINSIC
low complexity region	3192	3212	N/A	INTRINSIC
low complexity region	3299	3337	N/A	INTRINSIC
Pfam:SPOC	3465	3586	2.7e-29	PFAM

Predicted Effect

unknown
Transcript: ENSMUST00000105786
AA Change: E644K

SMART Domains

Protein: ENSMUSP00000101412
Gene: ENSMUSG00000040761
AA Change: E644K

Domain	Start	End	E-Value	Type
RRM	7	77	7.77e-12	SMART
low complexity region	109	121	N/A	INTRINSIC
low complexity region	235	257	N/A	INTRINSIC
low complexity region	262	311	N/A	INTRINSIC
RRM	338	411	8.6e-5	SMART
RRM	441	511	1.56e-16	SMART
RRM	520	587	1.84e-13	SMART
low complexity region	692	714	N/A	INTRINSIC
low complexity region	718	743	N/A	INTRINSIC
low complexity region	772	796	N/A	INTRINSIC
coiled coil region	823	848	N/A	INTRINSIC
low complexity region	853	864	N/A	INTRINSIC
internal_repeat_2	867	977	8.58e-5	PROSPERO
coiled coil region	1517	1545	N/A	INTRINSIC
low complexity region	1610	1650	N/A	INTRINSIC
low complexity region	1658	1664	N/A	INTRINSIC
low complexity region	1665	1694	N/A	INTRINSIC
low complexity region	1770	1781	N/A	INTRINSIC
low complexity region	1833	1846	N/A	INTRINSIC
low complexity region	1911	1926	N/A	INTRINSIC
low complexity region	1963	1978	N/A	INTRINSIC
low complexity region	2026	2035	N/A	INTRINSIC
internal_repeat_2	2038	2138	8.58e-5	PROSPERO
low complexity region	2150	2170	N/A	INTRINSIC
low complexity region	2192	2214	N/A	INTRINSIC
low complexity region	2230	2242	N/A	INTRINSIC
low complexity region	2327	2346	N/A	INTRINSIC
low complexity region	2355	2394	N/A	INTRINSIC
low complexity region	2419	2436	N/A	INTRINSIC
low complexity region	2541	2556	N/A	INTRINSIC
low complexity region	2568	2578	N/A	INTRINSIC
low complexity region	2719	2745	N/A	INTRINSIC
low complexity region	2954	2965	N/A	INTRINSIC
low complexity region	3017	3029	N/A	INTRINSIC
low complexity region	3215	3235	N/A	INTRINSIC
low complexity region	3322	3360	N/A	INTRINSIC
Pfam:SPOC	3488	3609	2.7e-29	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a hormone inducible transcriptional repressor. Repression of transcription by this gene product can occur through interactions with other repressors, by the recruitment of proteins involved in histone deacetylation, or through sequestration of transcriptional activators. The product of this gene contains a carboxy-terminal domain that permits binding to other corepressor proteins. This domain also permits interaction with members of the NuRD complex, a nucleosome remodeling protein complex that contains deacetylase activity. In addition, this repressor contains several RNA recognition motifs that confer binding to a steroid receptor RNA coactivator; this binding can modulate the activity of both liganded and nonliganded steroid receptors. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mutant mice die during late gestation exhibiting morphological abnormalities of the heart, pancreas, and liver. Inactivation of this gene also affects the differentiation of B cells. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 81 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A930017K11Rik	A	T	17: 25,946,711 (GRCm38)	V534D	probably damaging	Het
Abraxas2	T	C	7: 132,874,849 (GRCm38)	F106S	probably damaging	Het
Acsf3	A	G	8: 122,785,946 (GRCm38)	T369A	probably benign	Het
Adgrf5	G	A	17: 43,428,444 (GRCm38)		probably null	Het
Aff1	T	A	5: 103,847,092 (GRCm38)	I1052K	probably benign	Het
Akap8	T	C	17: 32,316,575 (GRCm38)	D155G	possibly damaging	Het
Alg10b	A	T	15: 90,227,450 (GRCm38)	M166L	probably benign	Het
Allc	T	A	12: 28,563,409 (GRCm38)	Q178L	possibly damaging	Het
Angptl4	C	A	17: 33,777,110 (GRCm38)	L297F	probably damaging	Het
Arfgap1	A	G	2: 180,971,076 (GRCm38)	K8E	possibly damaging	Het
Bckdhb	T	A	9: 84,010,326 (GRCm38)	V270E	possibly damaging	Het
Cbx3	T	C	6: 51,475,375 (GRCm38)		probably null	Het
Cd44	G	T	2: 102,834,262 (GRCm38)	N531K	probably benign	Het
Cdh23	A	T	10: 60,410,910 (GRCm38)	D916E	probably damaging	Het
Cr2	A	G	1: 195,155,286 (GRCm38)	V792A	probably benign	Het
Cstf3	T	C	2: 104,608,956 (GRCm38)	L38P	probably damaging	Het
Cyp2a5	A	G	7: 26,836,783 (GRCm38)	E151G	probably benign	Het
Dbr1	T	C	9: 99,582,549 (GRCm38)	F127S		Het
Ddit4	A	T	10: 59,951,495 (GRCm38)	D6E	probably damaging	Het
Disc1	G	C	8: 125,165,102 (GRCm38)	R631S	probably damaging	Het
Dnah5	T	A	15: 28,235,819 (GRCm38)		probably null	Het
Dnah9	T	C	11: 66,080,578 (GRCm38)	K1595E	probably damaging	Het
Dnmbp	T	C	19: 43,901,505 (GRCm38)	R608G	probably damaging	Het
Efcab5	G	C	11: 77,137,561 (GRCm38)	P315A	probably benign	Het
Eif4a2	A	G	16: 23,113,262 (GRCm38)	Y392C	possibly damaging	Het
F5	G	T	1: 164,192,708 (GRCm38)	K917N	probably benign	Het
Fig4	T	C	10: 41,251,756 (GRCm38)	M571V	probably benign	Het
Flot2	T	A	11: 78,057,976 (GRCm38)	I259N	probably damaging	Het
Gm8122	C	A	14: 43,230,601 (GRCm38)		probably null	Het
Gpr152	T	C	19: 4,142,964 (GRCm38)	V168A	possibly damaging	Het
H1f0	G	A	15: 79,028,903 (GRCm38)	G61D	probably damaging	Het
Ihh	T	A	1: 74,948,333 (GRCm38)	K183M	probably damaging	Het
Itga2	A	G	13: 114,837,202 (GRCm38)	L1116P	probably damaging	Het
Kcnj10	A	G	1: 172,369,260 (GRCm38)	T114A	possibly damaging	Het
Kcnk3	T	G	5: 30,621,966 (GRCm38)	L120R	probably damaging	Het
Krt14	C	A	11: 100,205,100 (GRCm38)	E211*	probably null	Het
Lars2	T	C	9: 123,427,480 (GRCm38)	L350P	probably damaging	Het
Lrba	T	C	3: 86,351,902 (GRCm38)	I1408T	probably damaging	Het
Lrrc27	A	G	7: 139,226,106 (GRCm38)	E229G	probably benign	Het
Lrrc8e	G	T	8: 4,235,626 (GRCm38)	R617L	probably benign	Het
M1ap	A	G	6: 82,981,949 (GRCm38)	D187G	probably benign	Het
March1	A	T	8: 66,468,399 (GRCm38)	K243*	probably null	Het
Med16	C	T	10: 79,903,197 (GRCm38)	V252M	probably damaging	Het
Micu3	T	C	8: 40,348,958 (GRCm38)	S189P	probably benign	Het
Mier2	C	A	10: 79,540,298 (GRCm38)	M264I	unknown	Het
Mindy4	A	T	6: 55,301,025 (GRCm38)	N618I	probably damaging	Het
Mmrn1	C	T	6: 60,976,336 (GRCm38)	Q534*	probably null	Het
Mroh9	C	T	1: 163,076,289 (GRCm38)		probably null	Het
Ms4a6d	G	A	19: 11,590,073 (GRCm38)	Q155*	probably null	Het
Mta3	T	A	17: 83,708,441 (GRCm38)	I24N	probably damaging	Het
Mtmr4	A	T	11: 87,600,650 (GRCm38)	H147L	probably damaging	Het
Nfam1	T	C	15: 83,010,439 (GRCm38)	K155R	probably benign	Het
Nlrp6	A	T	7: 140,921,278 (GRCm38)		probably benign	Het
Olfr1006	A	G	2: 85,674,845 (GRCm38)	F102S		Het
Olfr1153	T	C	2: 87,896,409 (GRCm38)		probably benign	Het
Olfr1164	A	T	2: 88,093,198 (GRCm38)	F246Y	probably benign	Het
Olfr213	A	G	6: 116,540,534 (GRCm38)	E27G	probably benign	Het
Olfr357	T	A	2: 36,996,861 (GRCm38)	I17N	possibly damaging	Het
P4ha1	A	G	10: 59,350,418 (GRCm38)	R240G	probably damaging	Het
Pla2r1	G	T	2: 60,458,379 (GRCm38)	D636E	probably benign	Het
Polr2a	A	G	11: 69,741,060 (GRCm38)	L1060P	possibly damaging	Het
Ptgdr2	C	A	19: 10,940,955 (GRCm38)	Q279K	probably benign	Het
Rab11fip2	T	A	19: 59,937,421 (GRCm38)	R121S	probably benign	Het
Rasa2	T	C	9: 96,544,355 (GRCm38)	S813G	probably benign	Het
Rassf5	T	C	1: 131,178,536 (GRCm38)	K411E	possibly damaging	Het
Reep4	T	C	14: 70,546,547 (GRCm38)	Y35H	probably damaging	Het
Ror1	A	T	4: 100,425,943 (GRCm38)	M402L	probably benign	Het
Slc32a1	A	G	2: 158,614,406 (GRCm38)	E327G	probably damaging	Het
Tarsl2	C	G	7: 65,658,924 (GRCm38)	Q281E	probably damaging	Het
Tcl1	A	G	12: 105,218,675 (GRCm38)	I92T	probably damaging	Het
Tifab	A	G	13: 56,176,307 (GRCm38)	S108P	probably damaging	Het
Traf1	T	C	2: 34,948,233 (GRCm38)	N198D	probably benign	Het
Trim35	T	C	14: 66,309,205 (GRCm38)	Y474H	probably damaging	Het
Trim54	T	A	5: 31,137,161 (GRCm38)	D344E	probably benign	Het
Ttc28	A	T	5: 111,226,037 (GRCm38)	H1113L	probably damaging	Het
Vcam1	T	A	3: 116,114,562 (GRCm38)	D617V	probably damaging	Het
Vmn2r81	A	T	10: 79,268,385 (GRCm38)	T281S	probably benign	Het
Whrn	C	T	4: 63,431,959 (GRCm38)	R507K	possibly damaging	Het
Zdhhc14	A	G	17: 5,726,876 (GRCm38)	S303G	probably benign	Het
Zfp292	A	T	4: 34,806,839 (GRCm38)	N2073K	probably benign	Het
Zgpat	T	A	2: 181,380,435 (GRCm38)	V163D		Het

Other mutations in Spen

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01133:Spen	APN	4	141,489,901 (GRCm38)	missense	unknown
IGL01357:Spen	APN	4	141,517,113 (GRCm38)	missense	unknown
IGL02184:Spen	APN	4	141,487,606 (GRCm38)	missense	unknown
IGL02226:Spen	APN	4	141,478,146 (GRCm38)	missense	unknown
IGL02321:Spen	APN	4	141,517,130 (GRCm38)	missense	unknown
IGL02350:Spen	APN	4	141,477,579 (GRCm38)	missense	unknown
IGL02357:Spen	APN	4	141,477,579 (GRCm38)	missense	unknown
IGL02627:Spen	APN	4	141,473,015 (GRCm38)	missense	probably damaging	0.99
IGL02683:Spen	APN	4	141,471,645 (GRCm38)	missense	probably benign	0.06
IGL02945:Spen	APN	4	141,494,313 (GRCm38)	missense	unknown
IGL02950:Spen	APN	4	141,469,508 (GRCm38)	missense	probably damaging	1.00
IGL03008:Spen	APN	4	141,476,137 (GRCm38)	missense	possibly damaging	0.70
IGL03019:Spen	APN	4	141,478,916 (GRCm38)	missense	unknown
IGL03038:Spen	APN	4	141,538,239 (GRCm38)	missense	unknown
IGL03334:Spen	APN	4	141,469,969 (GRCm38)	missense	probably damaging	1.00
filtered	UTSW	4	141,477,372 (GRCm38)	missense	unknown
mentholated	UTSW	4	141,469,400 (GRCm38)	missense	possibly damaging	0.78
R0105:Spen	UTSW	4	141,469,810 (GRCm38)	splice site	probably benign
R0268:Spen	UTSW	4	141,477,557 (GRCm38)	missense	unknown
R0359:Spen	UTSW	4	141,516,870 (GRCm38)	missense	unknown
R0394:Spen	UTSW	4	141,474,203 (GRCm38)	missense	probably benign	0.03
R0423:Spen	UTSW	4	141,479,336 (GRCm38)	missense	unknown
R0433:Spen	UTSW	4	141,483,758 (GRCm38)	missense	unknown
R0462:Spen	UTSW	4	141,473,651 (GRCm38)	missense	probably damaging	1.00
R0687:Spen	UTSW	4	141,488,028 (GRCm38)	missense	unknown
R0699:Spen	UTSW	4	141,474,391 (GRCm38)	missense	possibly damaging	0.72
R0865:Spen	UTSW	4	141,471,870 (GRCm38)	missense	probably benign	0.11
R0918:Spen	UTSW	4	141,485,564 (GRCm38)	missense	unknown
R1034:Spen	UTSW	4	141,475,752 (GRCm38)	missense	probably benign	0.33
R1341:Spen	UTSW	4	141,469,400 (GRCm38)	missense	possibly damaging	0.78
R1401:Spen	UTSW	4	141,471,821 (GRCm38)	missense	probably damaging	0.98
R1509:Spen	UTSW	4	141,475,635 (GRCm38)	missense	probably benign	0.00
R1509:Spen	UTSW	4	141,475,700 (GRCm38)	missense	possibly damaging	0.53
R1561:Spen	UTSW	4	141,472,383 (GRCm38)	nonsense	probably null
R1589:Spen	UTSW	4	141,488,024 (GRCm38)	missense	unknown
R1640:Spen	UTSW	4	141,468,943 (GRCm38)	missense	probably damaging	0.98
R1758:Spen	UTSW	4	141,476,375 (GRCm38)	missense	unknown
R1764:Spen	UTSW	4	141,472,950 (GRCm38)	missense	probably damaging	1.00
R1824:Spen	UTSW	4	141,472,785 (GRCm38)	missense	probably damaging	1.00
R1899:Spen	UTSW	4	141,470,343 (GRCm38)	missense	probably benign	0.17
R1916:Spen	UTSW	4	141,472,598 (GRCm38)	missense	probably damaging	1.00
R2011:Spen	UTSW	4	141,473,329 (GRCm38)	missense	probably damaging	1.00
R2295:Spen	UTSW	4	141,477,273 (GRCm38)	missense	unknown
R2379:Spen	UTSW	4	141,516,927 (GRCm38)	missense	unknown
R2404:Spen	UTSW	4	141,477,905 (GRCm38)	missense	unknown
R3719:Spen	UTSW	4	141,517,183 (GRCm38)	missense	unknown
R3889:Spen	UTSW	4	141,477,881 (GRCm38)	missense	unknown
R3945:Spen	UTSW	4	141,477,353 (GRCm38)	missense	unknown
R4227:Spen	UTSW	4	141,522,147 (GRCm38)	missense	unknown
R4326:Spen	UTSW	4	141,477,372 (GRCm38)	missense	unknown
R4382:Spen	UTSW	4	141,473,139 (GRCm38)	missense	possibly damaging	0.88
R4542:Spen	UTSW	4	141,476,786 (GRCm38)	missense	unknown
R4757:Spen	UTSW	4	141,473,079 (GRCm38)	nonsense	probably null
R4771:Spen	UTSW	4	141,472,596 (GRCm38)	missense	probably benign	0.14
R5072:Spen	UTSW	4	141,522,302 (GRCm38)	missense	unknown
R5121:Spen	UTSW	4	141,476,099 (GRCm38)	missense	probably benign	0.00
R5176:Spen	UTSW	4	141,476,276 (GRCm38)	missense	unknown
R5290:Spen	UTSW	4	141,473,816 (GRCm38)	missense	probably damaging	1.00
R5291:Spen	UTSW	4	141,488,079 (GRCm38)	missense	unknown
R5293:Spen	UTSW	4	141,472,406 (GRCm38)	missense	possibly damaging	0.89
R5347:Spen	UTSW	4	141,471,485 (GRCm38)	missense	probably benign	0.26
R5511:Spen	UTSW	4	141,516,838 (GRCm38)	missense	unknown
R5511:Spen	UTSW	4	141,475,064 (GRCm38)	missense	possibly damaging	0.86
R5772:Spen	UTSW	4	141,478,184 (GRCm38)	missense	unknown
R5834:Spen	UTSW	4	141,471,843 (GRCm38)	missense	possibly damaging	0.63
R5858:Spen	UTSW	4	141,473,871 (GRCm38)	missense	probably benign	0.05
R6214:Spen	UTSW	4	141,479,112 (GRCm38)	missense	unknown
R6232:Spen	UTSW	4	141,517,022 (GRCm38)	missense	unknown
R6345:Spen	UTSW	4	141,471,633 (GRCm38)	missense	possibly damaging	0.86
R6419:Spen	UTSW	4	141,476,310 (GRCm38)	missense	unknown
R6455:Spen	UTSW	4	141,475,509 (GRCm38)	missense	probably damaging	0.97
R6979:Spen	UTSW	4	141,478,063 (GRCm38)	missense	unknown
R6994:Spen	UTSW	4	141,493,459 (GRCm38)	missense	unknown
R7018:Spen	UTSW	4	141,493,444 (GRCm38)	missense	unknown
R7040:Spen	UTSW	4	141,494,382 (GRCm38)	missense	unknown
R7127:Spen	UTSW	4	141,476,108 (GRCm38)	missense	possibly damaging	0.53
R7218:Spen	UTSW	4	141,472,650 (GRCm38)	missense	possibly damaging	0.54
R7234:Spen	UTSW	4	141,479,135 (GRCm38)	missense	unknown
R7316:Spen	UTSW	4	141,477,054 (GRCm38)	missense	unknown
R7356:Spen	UTSW	4	141,471,924 (GRCm38)	nonsense	probably null
R7400:Spen	UTSW	4	141,473,741 (GRCm38)	missense	probably damaging	1.00
R7470:Spen	UTSW	4	141,479,294 (GRCm38)	missense	unknown
R7698:Spen	UTSW	4	141,472,845 (GRCm38)	missense	probably damaging	1.00
R7858:Spen	UTSW	4	141,488,131 (GRCm38)	splice site	probably null
R8033:Spen	UTSW	4	141,471,746 (GRCm38)	missense	probably benign	0.03
R8064:Spen	UTSW	4	141,475,700 (GRCm38)	missense	possibly damaging	0.53
R8159:Spen	UTSW	4	141,475,003 (GRCm38)	missense	possibly damaging	0.53
R8187:Spen	UTSW	4	141,472,905 (GRCm38)	missense	possibly damaging	0.93
R8463:Spen	UTSW	4	141,522,279 (GRCm38)	missense	unknown
R8557:Spen	UTSW	4	141,470,370 (GRCm38)	missense	probably benign	0.14
R8558:Spen	UTSW	4	141,470,370 (GRCm38)	missense	probably benign	0.14
R8672:Spen	UTSW	4	141,470,370 (GRCm38)	missense	probably benign	0.14
R8673:Spen	UTSW	4	141,470,370 (GRCm38)	missense	probably benign	0.14
R8674:Spen	UTSW	4	141,470,370 (GRCm38)	missense	probably benign	0.14
R8714:Spen	UTSW	4	141,488,003 (GRCm38)	missense	unknown
R8735:Spen	UTSW	4	141,469,818 (GRCm38)	missense	probably benign	0.32
R8762:Spen	UTSW	4	141,472,950 (GRCm38)	missense	probably damaging	1.00
R8877:Spen	UTSW	4	141,471,826 (GRCm38)	nonsense	probably null
R8878:Spen	UTSW	4	141,477,209 (GRCm38)	missense	unknown
R8937:Spen	UTSW	4	141,474,063 (GRCm38)	missense	probably damaging	1.00
R8939:Spen	UTSW	4	141,475,658 (GRCm38)	missense	possibly damaging	0.72
R8968:Spen	UTSW	4	141,470,390 (GRCm38)	missense	probably benign	0.02
R8971:Spen	UTSW	4	141,474,578 (GRCm38)	missense	possibly damaging	0.53
R9016:Spen	UTSW	4	141,473,627 (GRCm38)	missense	probably damaging	1.00
R9072:Spen	UTSW	4	141,476,391 (GRCm38)	missense	unknown
R9073:Spen	UTSW	4	141,476,391 (GRCm38)	missense	unknown
R9120:Spen	UTSW	4	141,472,922 (GRCm38)	missense
R9136:Spen	UTSW	4	141,522,312 (GRCm38)	missense	unknown
R9138:Spen	UTSW	4	141,469,486 (GRCm38)	missense	probably damaging	1.00
R9150:Spen	UTSW	4	141,517,157 (GRCm38)	missense	unknown
R9225:Spen	UTSW	4	141,475,632 (GRCm38)	missense	possibly damaging	0.53
R9492:Spen	UTSW	4	141,471,787 (GRCm38)	missense	probably benign	0.26
R9537:Spen	UTSW	4	141,516,845 (GRCm38)	small deletion	probably benign
R9537:Spen	UTSW	4	141,471,704 (GRCm38)	missense	probably benign	0.15
R9602:Spen	UTSW	4	141,477,872 (GRCm38)	missense	unknown
R9609:Spen	UTSW	4	141,488,108 (GRCm38)	missense	unknown
R9686:Spen	UTSW	4	141,472,635 (GRCm38)	missense	probably benign	0.27
R9697:Spen	UTSW	4	141,468,964 (GRCm38)	missense	probably damaging	1.00
R9713:Spen	UTSW	4	141,517,020 (GRCm38)	missense	unknown
T0722:Spen	UTSW	4	141,474,353 (GRCm38)	missense	probably benign	0.33
T0975:Spen	UTSW	4	141,474,353 (GRCm38)	missense	probably benign	0.33
Z1088:Spen	UTSW	4	141,477,976 (GRCm38)	missense	unknown
Z1088:Spen	UTSW	4	141,477,977 (GRCm38)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TGTATTCGCGAGGCTCATCATAG -3'
(R):5'- GCCAAGTGACTACGTAAGCAC -3'

Sequencing Primer
(F):5'- GTACCGGGAGTCATAATACTCTC -3'
(R):5'- CAGAGCTGCCTGTGTAAGAGAATTTC -3'

Posted On

2019-09-13