Mutagenetix > Incidental Mutation

Incidental Mutation 'R7350:Vmn2r81'

570516

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r81

Ensembl Gene

ENSMUSG00000055515

Gene Name

vomeronasal 2, receptor 81

Synonyms

V2rf2, EC1-VR2, pheromone recepter

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R7350 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

79247777-79294535 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 79268385 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 281 (T281S)

Ref Sequence

ENSEMBL: ENSMUSP00000020547 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020547]

AlphaFold

Q80Z09

Predicted Effect

probably benign
Transcript: ENSMUST00000020547
AA Change: T281S

PolyPhen 2 Score 0.032 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000020547
Gene: ENSMUSG00000055515
AA Change: T281S

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	79	474	7.7e-37	PFAM
Pfam:NCD3G	517	570	8.9e-21	PFAM
Pfam:7tm_3	603	838	6.1e-50	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit vomeronasal sensory neuron axons that spread out diffusely over the posterior accessory olfactory bulb and extend abnormally into the anterior olfactory bulb. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 81 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A930017K11Rik	A	T	17: 25,946,711	V534D	probably damaging	Het
Abraxas2	T	C	7: 132,874,849	F106S	probably damaging	Het
Acsf3	A	G	8: 122,785,946	T369A	probably benign	Het
Adgrf5	G	A	17: 43,428,444		probably null	Het
Aff1	T	A	5: 103,847,092	I1052K	probably benign	Het
Akap8	T	C	17: 32,316,575	D155G	possibly damaging	Het
Alg10b	A	T	15: 90,227,450	M166L	probably benign	Het
Allc	T	A	12: 28,563,409	Q178L	possibly damaging	Het
Angptl4	C	A	17: 33,777,110	L297F	probably damaging	Het
Arfgap1	A	G	2: 180,971,076	K8E	possibly damaging	Het
Bckdhb	T	A	9: 84,010,326	V270E	possibly damaging	Het
Cbx3	T	C	6: 51,475,375		probably null	Het
Cd44	G	T	2: 102,834,262	N531K	probably benign	Het
Cdh23	A	T	10: 60,410,910	D916E	probably damaging	Het
Cr2	A	G	1: 195,155,286	V792A	probably benign	Het
Cstf3	T	C	2: 104,608,956	L38P	probably damaging	Het
Cyp2a5	A	G	7: 26,836,783	E151G	probably benign	Het
Dbr1	T	C	9: 99,582,549	F127S		Het
Ddit4	A	T	10: 59,951,495	D6E	probably damaging	Het
Disc1	G	C	8: 125,165,102	R631S	probably damaging	Het
Dnah5	T	A	15: 28,235,819		probably null	Het
Dnah9	T	C	11: 66,080,578	K1595E	probably damaging	Het
Dnmbp	T	C	19: 43,901,505	R608G	probably damaging	Het
Efcab5	G	C	11: 77,137,561	P315A	probably benign	Het
Eif4a2	A	G	16: 23,113,262	Y392C	possibly damaging	Het
F5	G	T	1: 164,192,708	K917N	probably benign	Het
Fig4	T	C	10: 41,251,756	M571V	probably benign	Het
Flot2	T	A	11: 78,057,976	I259N	probably damaging	Het
Gm8122	C	A	14: 43,230,601		probably null	Het
Gpr152	T	C	19: 4,142,964	V168A	possibly damaging	Het
H1f0	G	A	15: 79,028,903	G61D	probably damaging	Het
Ihh	T	A	1: 74,948,333	K183M	probably damaging	Het
Itga2	A	G	13: 114,837,202	L1116P	probably damaging	Het
Kcnj10	A	G	1: 172,369,260	T114A	possibly damaging	Het
Kcnk3	T	G	5: 30,621,966	L120R	probably damaging	Het
Krt14	C	A	11: 100,205,100	E211*	probably null	Het
Lars2	T	C	9: 123,427,480	L350P	probably damaging	Het
Lrba	T	C	3: 86,351,902	I1408T	probably damaging	Het
Lrrc27	A	G	7: 139,226,106	E229G	probably benign	Het
Lrrc8e	G	T	8: 4,235,626	R617L	probably benign	Het
M1ap	A	G	6: 82,981,949	D187G	probably benign	Het
March1	A	T	8: 66,468,399	K243*	probably null	Het
Med16	C	T	10: 79,903,197	V252M	probably damaging	Het
Micu3	T	C	8: 40,348,958	S189P	probably benign	Het
Mier2	C	A	10: 79,540,298	M264I	unknown	Het
Mindy4	A	T	6: 55,301,025	N618I	probably damaging	Het
Mmrn1	C	T	6: 60,976,336	Q534*	probably null	Het
Mroh9	C	T	1: 163,076,289		probably null	Het
Ms4a6d	G	A	19: 11,590,073	Q155*	probably null	Het
Mta3	T	A	17: 83,708,441	I24N	probably damaging	Het
Mtmr4	A	T	11: 87,600,650	H147L	probably damaging	Het
Nfam1	T	C	15: 83,010,439	K155R	probably benign	Het
Nlrp6	A	T	7: 140,921,278		probably benign	Het
Olfr1006	A	G	2: 85,674,845	F102S		Het
Olfr1153	T	C	2: 87,896,409		probably benign	Het
Olfr1164	A	T	2: 88,093,198	F246Y	probably benign	Het
Olfr213	A	G	6: 116,540,534	E27G	probably benign	Het
Olfr357	T	A	2: 36,996,861	I17N	possibly damaging	Het
P4ha1	A	G	10: 59,350,418	R240G	probably damaging	Het
Pla2r1	G	T	2: 60,458,379	D636E	probably benign	Het
Polr2a	A	G	11: 69,741,060	L1060P	possibly damaging	Het
Ptgdr2	C	A	19: 10,940,955	Q279K	probably benign	Het
Rab11fip2	T	A	19: 59,937,421	R121S	probably benign	Het
Rasa2	T	C	9: 96,544,355	S813G	probably benign	Het
Rassf5	T	C	1: 131,178,536	K411E	possibly damaging	Het
Reep4	T	C	14: 70,546,547	Y35H	probably damaging	Het
Ror1	A	T	4: 100,425,943	M402L	probably benign	Het
Slc32a1	A	G	2: 158,614,406	E327G	probably damaging	Het
Spen	C	T	4: 141,479,385	E644K	unknown	Het
Tarsl2	C	G	7: 65,658,924	Q281E	probably damaging	Het
Tcl1	A	G	12: 105,218,675	I92T	probably damaging	Het
Tifab	A	G	13: 56,176,307	S108P	probably damaging	Het
Traf1	T	C	2: 34,948,233	N198D	probably benign	Het
Trim35	T	C	14: 66,309,205	Y474H	probably damaging	Het
Trim54	T	A	5: 31,137,161	D344E	probably benign	Het
Ttc28	A	T	5: 111,226,037	H1113L	probably damaging	Het
Vcam1	T	A	3: 116,114,562	D617V	probably damaging	Het
Whrn	C	T	4: 63,431,959	R507K	possibly damaging	Het
Zdhhc14	A	G	17: 5,726,876	S303G	probably benign	Het
Zfp292	A	T	4: 34,806,839	N2073K	probably benign	Het
Zgpat	T	A	2: 181,380,435	V163D		Het

Other mutations in Vmn2r81

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01111:Vmn2r81	APN	10	79247997	missense	probably benign	0.20
IGL02255:Vmn2r81	APN	10	79247972	nonsense	probably null
IGL02901:Vmn2r81	APN	10	79270730	missense	probably damaging	0.98
R0383:Vmn2r81	UTSW	10	79293447	missense	possibly damaging	0.95
R0555:Vmn2r81	UTSW	10	79293449	missense	probably damaging	1.00
R0715:Vmn2r81	UTSW	10	79268600	missense	probably damaging	1.00
R0967:Vmn2r81	UTSW	10	79248023	splice site	probably benign
R1438:Vmn2r81	UTSW	10	79293857	missense	probably benign	0.00
R1468:Vmn2r81	UTSW	10	79293662	missense	probably damaging	1.00
R1468:Vmn2r81	UTSW	10	79293662	missense	probably damaging	1.00
R1589:Vmn2r81	UTSW	10	79293024	missense	probably damaging	0.99
R1671:Vmn2r81	UTSW	10	79267431	missense	probably benign	0.04
R1672:Vmn2r81	UTSW	10	79268278	missense	probably damaging	0.99
R1728:Vmn2r81	UTSW	10	79270655	missense	probably benign	0.08
R1784:Vmn2r81	UTSW	10	79270655	missense	probably benign	0.08
R1931:Vmn2r81	UTSW	10	79293494	missense	probably damaging	0.98
R1934:Vmn2r81	UTSW	10	79247794	start codon destroyed	probably null	0.71
R1944:Vmn2r81	UTSW	10	79293737	missense	probably damaging	1.00
R2102:Vmn2r81	UTSW	10	79293500	missense	probably damaging	0.97
R2190:Vmn2r81	UTSW	10	79268251	missense	possibly damaging	0.67
R2392:Vmn2r81	UTSW	10	79274682	missense	probably damaging	1.00
R3770:Vmn2r81	UTSW	10	79270600	missense	probably damaging	1.00
R4551:Vmn2r81	UTSW	10	79268407	missense	possibly damaging	0.95
R4629:Vmn2r81	UTSW	10	79267442	missense	probably damaging	0.99
R4691:Vmn2r81	UTSW	10	79293377	nonsense	probably null
R4829:Vmn2r81	UTSW	10	79247801	missense	possibly damaging	0.48
R4914:Vmn2r81	UTSW	10	79270523	missense	probably null	1.00
R4996:Vmn2r81	UTSW	10	79293413	missense	probably benign	0.04
R5242:Vmn2r81	UTSW	10	79293475	nonsense	probably null
R5253:Vmn2r81	UTSW	10	79247986	missense	probably benign	0.00
R5925:Vmn2r81	UTSW	10	79247803	missense	probably damaging	1.00
R6169:Vmn2r81	UTSW	10	79268548	missense	probably benign
R6270:Vmn2r81	UTSW	10	79293815	missense	probably benign
R6525:Vmn2r81	UTSW	10	79293726	missense	probably benign	0.11
R6813:Vmn2r81	UTSW	10	79268605	missense	probably benign	0.00
R6886:Vmn2r81	UTSW	10	79268154	missense	possibly damaging	0.90
R7200:Vmn2r81	UTSW	10	79270736	critical splice donor site	probably null
R7504:Vmn2r81	UTSW	10	79268332	missense	probably benign
R7832:Vmn2r81	UTSW	10	79293830	missense	probably damaging	1.00
R8130:Vmn2r81	UTSW	10	79274704	missense	possibly damaging	0.95
R8362:Vmn2r81	UTSW	10	79267449	missense	probably benign	0.17
R8407:Vmn2r81	UTSW	10	79268194	missense	possibly damaging	0.92
R8540:Vmn2r81	UTSW	10	79293231	missense	probably damaging	0.99
R8558:Vmn2r81	UTSW	10	79270633	missense	possibly damaging	0.94
R8700:Vmn2r81	UTSW	10	79293683	missense	probably damaging	1.00
R8779:Vmn2r81	UTSW	10	79267384	missense	possibly damaging	0.78
R8987:Vmn2r81	UTSW	10	79293870	missense	probably damaging	1.00
R9001:Vmn2r81	UTSW	10	79268270	missense	possibly damaging	0.73
R9055:Vmn2r81	UTSW	10	79274607	missense	probably benign
R9145:Vmn2r81	UTSW	10	79268194	missense	possibly damaging	0.92
R9151:Vmn2r81	UTSW	10	79268071	missense
R9253:Vmn2r81	UTSW	10	79293748	missense	probably damaging	1.00
R9255:Vmn2r81	UTSW	10	79267332	missense	possibly damaging	0.77
R9370:Vmn2r81	UTSW	10	79268590	missense	probably damaging	1.00
R9588:Vmn2r81	UTSW	10	79293371	missense	possibly damaging	0.46
X0067:Vmn2r81	UTSW	10	79293043	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- GAGGTGCTCAGTTTCTATCAGAC -3'
(R):5'- GCAGTTGAAATGCCAGTACC -3'

Sequencing Primer
(F):5'- GTGCTCAGTTTCTATCAGACTTTAG -3'
(R):5'- AAAATATCTTCTGGGTACTTGATGGG -3'

Posted On

2019-09-13