Mutagenetix > Incidental Mutation

Incidental Mutation 'R7352:Map4k4'

570606

Institutional Source

Beutler Lab

Gene Symbol

Map4k4

Ensembl Gene

ENSMUSG00000026074

Gene Name

mitogen-activated protein kinase kinase kinase kinase 4

Synonyms

9430080K19Rik, Nik

MMRRC Submission

045438-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7352 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

39900913-40026310 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 39962227 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Aspartic acid at position 58 (V58D)

Ref Sequence

ENSEMBL: ENSMUSP00000141862 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000163854] [ENSMUST00000168431] [ENSMUST00000191761] [ENSMUST00000192509] [ENSMUST00000193682] [ENSMUST00000195259] [ENSMUST00000195636] [ENSMUST00000195860]

AlphaFold

no structure available at present

Predicted Effect

unknown
Transcript: ENSMUST00000163854
AA Change: V58D

SMART Domains

Protein: ENSMUSP00000126961
Gene: ENSMUSG00000026074
AA Change: V58D

Domain	Start	End	E-Value	Type
S_TKc	25	289	6.87e-95	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	494	N/A	INTRINSIC
low complexity region	503	512	N/A	INTRINSIC
low complexity region	687	698	N/A	INTRINSIC
low complexity region	721	747	N/A	INTRINSIC
low complexity region	754	763	N/A	INTRINSIC
low complexity region	811	837	N/A	INTRINSIC
low complexity region	891	904	N/A	INTRINSIC
low complexity region	919	929	N/A	INTRINSIC
CNH	970	1268	2.76e-127	SMART

Predicted Effect

SMART Domains

Protein: ENSMUSP00000129796
Gene: ENSMUSG00000026074
AA Change: V58D

Domain	Start	End	E-Value	Type
S_TKc	25	289	6.87e-95	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	494	N/A	INTRINSIC
low complexity region	503	512	N/A	INTRINSIC
low complexity region	633	644	N/A	INTRINSIC
low complexity region	667	693	N/A	INTRINSIC
low complexity region	700	709	N/A	INTRINSIC
low complexity region	757	783	N/A	INTRINSIC
low complexity region	837	850	N/A	INTRINSIC
low complexity region	865	875	N/A	INTRINSIC
CNH	916	1214	2.76e-127	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000191761
AA Change: V58D

SMART Domains

Protein: ENSMUSP00000141332
Gene: ENSMUSG00000026074
AA Change: V58D

Domain	Start	End	E-Value	Type
S_TKc	25	289	3.4e-97	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	404	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000192509
AA Change: V58D

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000141665
Gene: ENSMUSG00000026074
AA Change: V58D

Domain	Start	End	E-Value	Type
S_TKc	25	289	6.87e-95	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	494	N/A	INTRINSIC
low complexity region	503	512	N/A	INTRINSIC
low complexity region	633	644	N/A	INTRINSIC
low complexity region	667	693	N/A	INTRINSIC
low complexity region	700	709	N/A	INTRINSIC
low complexity region	757	783	N/A	INTRINSIC
low complexity region	837	850	N/A	INTRINSIC
low complexity region	865	875	N/A	INTRINSIC
CNH	916	1214	2.76e-127	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000193682
AA Change: V58D

SMART Domains

Protein: ENSMUSP00000141862
Gene: ENSMUSG00000026074
AA Change: V58D

Domain	Start	End	E-Value	Type
S_TKc	25	289	6.87e-95	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	494	N/A	INTRINSIC
low complexity region	503	512	N/A	INTRINSIC
low complexity region	556	567	N/A	INTRINSIC
low complexity region	590	616	N/A	INTRINSIC
low complexity region	623	632	N/A	INTRINSIC
low complexity region	680	706	N/A	INTRINSIC
low complexity region	785	814	N/A	INTRINSIC
low complexity region	824	837	N/A	INTRINSIC
low complexity region	852	862	N/A	INTRINSIC
CNH	903	1201	2.76e-127	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000195259
AA Change: V58D

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000142056
Gene: ENSMUSG00000026074
AA Change: V58D

Domain	Start	End	E-Value	Type
S_TKc	25	289	6.87e-95	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	494	N/A	INTRINSIC
low complexity region	503	512	N/A	INTRINSIC
low complexity region	610	621	N/A	INTRINSIC
low complexity region	644	670	N/A	INTRINSIC
low complexity region	677	686	N/A	INTRINSIC
low complexity region	731	757	N/A	INTRINSIC
low complexity region	811	824	N/A	INTRINSIC
low complexity region	839	849	N/A	INTRINSIC
CNH	890	1188	2.76e-127	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000195636
AA Change: V58D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000141613
Gene: ENSMUSG00000026074
AA Change: V58D

Domain	Start	End	E-Value	Type
S_TKc	25	289	3.4e-97	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	494	N/A	INTRINSIC
low complexity region	503	512	N/A	INTRINSIC
low complexity region	610	621	N/A	INTRINSIC
low complexity region	644	670	N/A	INTRINSIC
low complexity region	677	686	N/A	INTRINSIC
low complexity region	731	757	N/A	INTRINSIC
low complexity region	836	865	N/A	INTRINSIC
low complexity region	875	888	N/A	INTRINSIC
low complexity region	903	913	N/A	INTRINSIC
CNH	954	1252	1.4e-129	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000195860
AA Change: V58D

SMART Domains

Protein: ENSMUSP00000141400
Gene: ENSMUSG00000026074
AA Change: V58D

Domain	Start	End	E-Value	Type
S_TKc	25	289	6.87e-95	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	494	N/A	INTRINSIC
low complexity region	503	512	N/A	INTRINSIC
low complexity region	687	698	N/A	INTRINSIC
low complexity region	721	747	N/A	INTRINSIC
low complexity region	754	763	N/A	INTRINSIC
low complexity region	811	837	N/A	INTRINSIC
low complexity region	891	904	N/A	INTRINSIC
low complexity region	919	929	N/A	INTRINSIC
CNH	970	1268	2.76e-127	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for disruptions in this gene die as embryos around day E9.5-10.5. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 100 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
AA792892	A	T	5: 94,383,884 (GRCm38)	N209I	probably damaging	Het
Abca17	C	T	17: 24,289,054 (GRCm38)	W1124*	probably null	Het
Acap3	G	A	4: 155,905,711 (GRCm38)	V783I	possibly damaging	Het
Alkbh8	A	G	9: 3,345,796 (GRCm38)	K172R	probably damaging	Het
Ankrd28	A	G	14: 31,708,041 (GRCm38)	L860P	probably damaging	Het
Arap3	G	A	18: 37,973,278 (GRCm38)	T1504I	probably benign	Het
Atad5	T	C	11: 80,103,343 (GRCm38)		probably null	Het
Atp8a2	A	G	14: 59,791,204 (GRCm38)	V938A	probably benign	Het
AW551984	T	C	9: 39,592,925 (GRCm38)	M582V	probably benign	Het
Axdnd1	C	T	1: 156,382,477 (GRCm38)	V408I	possibly damaging	Het
Babam2	T	A	5: 32,007,250 (GRCm38)	Y326*	probably null	Het
Baiap2l1	T	C	5: 144,324,626 (GRCm38)	M20V	probably benign	Het
Bex6	A	G	16: 32,186,216 (GRCm38)		probably benign	Het
Bicc1	G	T	10: 70,947,900 (GRCm38)	T469K	probably benign	Het
Cad	T	A	5: 31,058,078 (GRCm38)	S78T	probably damaging	Het
Ccdc171	T	A	4: 83,818,023 (GRCm38)	D1210E	possibly damaging	Het
Ccnd1	T	C	7: 144,937,387 (GRCm38)	I161V	possibly damaging	Het
Cdc42bpb	A	T	12: 111,299,311 (GRCm38)	F1396I	probably damaging	Het
Cep170	A	T	1: 176,769,857 (GRCm38)	D373E	probably benign	Het
Chrna4	A	T	2: 181,037,474 (GRCm38)	I60N	probably damaging	Het
Cntn2	T	C	1: 132,522,399 (GRCm38)	T586A	probably benign	Het
Csmd2	G	T	4: 128,557,636 (GRCm38)	V3396F		Het
Cyp3a44	T	A	5: 145,803,688 (GRCm38)	L47F	probably benign	Het
D7Ertd443e	A	T	7: 134,349,394 (GRCm38)	H183Q	probably benign	Het
Ddx27	T	C	2: 167,029,513 (GRCm38)	V510A	probably benign	Het
Ddx58	T	A	4: 40,239,668 (GRCm38)	Q10L	probably benign	Het
Dgat1	G	T	15: 76,502,824 (GRCm38)	C396*	probably null	Het
Dmpk	A	G	7: 19,086,072 (GRCm38)	S83G	probably damaging	Het
Dsc2	T	G	18: 20,035,335 (GRCm38)	D689A	probably benign	Het
Eppk1	A	T	15: 76,106,418 (GRCm38)	W2088R	probably benign	Het
Exph5	A	C	9: 53,375,722 (GRCm38)	I1368L	probably benign	Het
Fadd	T	C	7: 144,580,659 (GRCm38)	K163R	probably benign	Het
Fam149a	T	A	8: 45,340,997 (GRCm38)	Q759L	probably damaging	Het
Foxo6	A	T	4: 120,268,251 (GRCm38)	M449K	probably benign	Het
Gad1	T	C	2: 70,594,750 (GRCm38)	F463L	probably benign	Het
Gad2	T	A	2: 22,623,823 (GRCm38)	F91I	probably benign	Het
Gcc2	T	C	10: 58,280,698 (GRCm38)		probably null	Het
Gjc1	A	C	11: 102,800,452 (GRCm38)	C242G	probably damaging	Het
Gkn1	T	C	6: 87,348,118 (GRCm38)	E103G	possibly damaging	Het
Gm867	T	C	10: 75,939,836 (GRCm38)	T39A	probably benign	Het
Gpr108	A	G	17: 57,236,944 (GRCm38)	Y421H	probably damaging	Het
Gramd1b	A	C	9: 40,307,993 (GRCm38)	V508G	probably damaging	Het
Grm1	A	T	10: 10,719,493 (GRCm38)	I797N	probably damaging	Het
Ice1	G	A	13: 70,606,102 (GRCm38)	Q622*	probably null	Het
Kcnb2	T	A	1: 15,710,611 (GRCm38)	V569E	probably benign	Het
Ksr2	T	G	5: 117,689,641 (GRCm38)	I495S	probably benign	Het
Litaf	A	T	16: 10,963,353 (GRCm38)	M94K	probably damaging	Het
Lmod2	T	C	6: 24,598,111 (GRCm38)	S77P	possibly damaging	Het
Lrp2	G	T	2: 69,472,397 (GRCm38)	S2996Y	probably benign	Het
Ltf	T	C	9: 111,028,450 (GRCm38)	S436P	probably benign	Het
Mapkapk3	C	A	9: 107,257,070 (GRCm38)	D332Y	possibly damaging	Het
Mroh1	C	A	15: 76,451,474 (GRCm38)	P1439Q	probably benign	Het
Ncor1	A	G	11: 62,333,911 (GRCm38)	V841A	probably damaging	Het
Nrxn3	A	G	12: 88,850,293 (GRCm38)	M248V	probably benign	Het
Olfr1000	T	C	2: 85,608,784 (GRCm38)	N42S	probably damaging	Het
Olfr1208	T	A	2: 88,896,718 (GRCm38)	N293I	probably damaging	Het
Olfr1238	T	C	2: 89,406,462 (GRCm38)	M206V	probably benign	Het
Olfr1239	C	A	2: 89,417,967 (GRCm38)	G149W	probably damaging	Het
Olfr1443	A	G	19: 12,680,751 (GRCm38)	I214M	possibly damaging	Het
Olfr523	A	T	7: 140,176,525 (GRCm38)	Y135F	probably damaging	Het
Olig2	A	G	16: 91,226,689 (GRCm38)	D97G	probably benign	Het
Pcdha11	T	A	18: 37,006,845 (GRCm38)	V509E	probably damaging	Het
Pglyrp3	G	A	3: 92,014,640 (GRCm38)	W5*	probably null	Het
Phf14	A	T	6: 11,961,638 (GRCm38)	N425Y	probably damaging	Het
Phf3	G	T	1: 30,804,326 (GRCm38)	R1851S	possibly damaging	Het
Pigs	A	G	11: 78,328,812 (GRCm38)	T39A	probably damaging	Het
Plin4	A	G	17: 56,104,427 (GRCm38)	M868T	probably benign	Het
Pnma2	A	G	14: 66,916,421 (GRCm38)	Q98R	possibly damaging	Het
Ppp4r3a	G	A	12: 101,041,832 (GRCm38)	T782I	probably damaging	Het
Prickle2	T	G	6: 92,410,890 (GRCm38)	E566D	probably benign	Het
Pvr	A	G	7: 19,910,616 (GRCm38)	I331T	possibly damaging	Het
Ranbp3l	A	T	15: 8,968,358 (GRCm38)		probably benign	Het
Rapgef4	T	A	2: 72,180,091 (GRCm38)	I331N	probably damaging	Het
Reg3g	C	T	6: 78,466,859 (GRCm38)	W122*	probably null	Het
Rlbp1	A	T	7: 79,381,680 (GRCm38)	I100N	probably damaging	Het
Rnf213	T	A	11: 119,443,579 (GRCm38)	Y3206N		Het
Rsf1	CGGCGGCGG	CGGCGGCGGGGGCGGCGG	7: 97,579,926 (GRCm38)		probably benign	Het
Rtl6	G	T	15: 84,556,980 (GRCm38)	R72S	possibly damaging	Het
Rufy3	T	A	5: 88,637,194 (GRCm38)	D408E	possibly damaging	Het
Scn3a	T	C	2: 65,525,701 (GRCm38)	I230V	possibly damaging	Het
Slc23a4	A	T	6: 34,948,279 (GRCm38)	I507N	possibly damaging	Het
Slc4a8	A	G	15: 100,790,984 (GRCm38)	T392A	probably damaging	Het
Spaca7	T	A	8: 12,585,742 (GRCm38)	I86K	probably benign	Het
Syt10	G	T	15: 89,814,456 (GRCm38)	D228E	probably benign	Het
Tbx3	T	A	5: 119,677,560 (GRCm38)	N308K	probably benign	Het
Telo2	A	G	17: 25,102,069 (GRCm38)	V724A	probably damaging	Het
Tfip11	T	C	5: 112,333,268 (GRCm38)	V370A	probably benign	Het
Thbs3	A	G	3: 89,225,280 (GRCm38)	E843G	probably benign	Het
Tmprss11g	T	C	5: 86,496,542 (GRCm38)	I148V	not run	Het
Trbv14	T	A	6: 41,135,495 (GRCm38)	D75E	possibly damaging	Het
Trio	A	G	15: 27,732,876 (GRCm38)	V2973A	probably damaging	Het
Ttn	T	C	2: 76,794,484 (GRCm38)	D15250G	probably damaging	Het
Ubr2	G	A	17: 46,930,426 (GRCm38)	T1734I	probably benign	Het
Ush2a	G	T	1: 188,466,124 (GRCm38)	A1462S	probably benign	Het
Ushbp1	T	G	8: 71,388,881 (GRCm38)	T435P	possibly damaging	Het
Vps13c	T	C	9: 67,840,446 (GRCm38)	V7A	possibly damaging	Het
Wnk4	T	C	11: 101,264,418 (GRCm38)	V385A	probably damaging	Het
Wnt2b	C	T	3: 104,947,177 (GRCm38)	V322I	probably benign	Het
Zbtb8a	C	T	4: 129,360,081 (GRCm38)	V207M	probably benign	Het
Zc3hav1l	A	G	6: 38,298,981 (GRCm38)	M87T	probably benign	Het

Other mutations in Map4k4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00328:Map4k4	APN	1	40,004,816 (GRCm38)	missense	probably damaging	0.99
IGL00417:Map4k4	APN	1	40,014,532 (GRCm38)	missense	possibly damaging	0.92
IGL00516:Map4k4	APN	1	40,014,602 (GRCm38)	missense	probably damaging	1.00
IGL01545:Map4k4	APN	1	40,014,229 (GRCm38)	splice site	probably benign
IGL02092:Map4k4	APN	1	40,024,348 (GRCm38)	missense	probably damaging	1.00
IGL02092:Map4k4	APN	1	39,986,783 (GRCm38)	missense	probably benign	0.12
IGL02570:Map4k4	APN	1	39,980,579 (GRCm38)	missense	probably benign	0.06
IGL02626:Map4k4	APN	1	40,014,097 (GRCm38)	splice site	probably benign
IGL02993:Map4k4	APN	1	40,014,188 (GRCm38)	missense	probably damaging	0.98
IGL03178:Map4k4	APN	1	39,986,693 (GRCm38)	missense	possibly damaging	0.63
tank	UTSW	1	40,004,864 (GRCm38)	missense	possibly damaging	0.93
IGL02835:Map4k4	UTSW	1	40,010,600 (GRCm38)	missense	probably damaging	0.99
R0496:Map4k4	UTSW	1	40,006,822 (GRCm38)	missense	probably damaging	0.99
R0498:Map4k4	UTSW	1	39,990,178 (GRCm38)	missense	probably benign	0.22
R0588:Map4k4	UTSW	1	40,004,864 (GRCm38)	missense	possibly damaging	0.93
R0674:Map4k4	UTSW	1	40,003,815 (GRCm38)	missense	probably damaging	1.00
R1205:Map4k4	UTSW	1	40,003,844 (GRCm38)	missense	probably damaging	1.00
R1349:Map4k4	UTSW	1	40,021,159 (GRCm38)	missense	probably damaging	1.00
R1615:Map4k4	UTSW	1	40,006,830 (GRCm38)	splice site	probably benign
R1763:Map4k4	UTSW	1	40,000,757 (GRCm38)	splice site	probably benign
R1800:Map4k4	UTSW	1	40,023,460 (GRCm38)	missense	probably damaging	1.00
R1893:Map4k4	UTSW	1	40,001,557 (GRCm38)	missense	probably benign	0.08
R2411:Map4k4	UTSW	1	40,007,496 (GRCm38)	missense	probably damaging	0.96
R2851:Map4k4	UTSW	1	40,000,755 (GRCm38)	splice site	probably benign
R2852:Map4k4	UTSW	1	40,000,755 (GRCm38)	splice site	probably benign
R2987:Map4k4	UTSW	1	39,986,765 (GRCm38)	missense	probably damaging	1.00
R3087:Map4k4	UTSW	1	40,021,082 (GRCm38)	critical splice acceptor site	probably null
R3688:Map4k4	UTSW	1	39,985,171 (GRCm38)	splice site	probably null
R4075:Map4k4	UTSW	1	40,023,462 (GRCm38)	missense	probably damaging	0.96
R4304:Map4k4	UTSW	1	39,973,972 (GRCm38)	missense	possibly damaging	0.74
R4564:Map4k4	UTSW	1	39,988,975 (GRCm38)	missense	probably damaging	1.00
R4569:Map4k4	UTSW	1	40,000,538 (GRCm38)	missense	probably damaging	1.00
R4613:Map4k4	UTSW	1	40,017,191 (GRCm38)	missense	probably benign	0.05
R4715:Map4k4	UTSW	1	40,019,564 (GRCm38)	missense	probably damaging	1.00
R4788:Map4k4	UTSW	1	40,003,916 (GRCm38)	missense	probably benign	0.01
R4926:Map4k4	UTSW	1	40,017,225 (GRCm38)	missense	probably damaging	1.00
R4943:Map4k4	UTSW	1	40,019,594 (GRCm38)	missense	probably damaging	0.99
R5033:Map4k4	UTSW	1	40,007,502 (GRCm38)	missense	probably damaging	0.99
R5177:Map4k4	UTSW	1	39,986,762 (GRCm38)	missense	probably damaging	1.00
R5297:Map4k4	UTSW	1	39,962,217 (GRCm38)	missense	probably damaging	1.00
R5844:Map4k4	UTSW	1	39,999,876 (GRCm38)	splice site	probably benign
R5952:Map4k4	UTSW	1	39,999,922 (GRCm38)	unclassified	probably benign
R6111:Map4k4	UTSW	1	40,011,662 (GRCm38)	missense	probably benign	0.00
R6125:Map4k4	UTSW	1	40,003,965 (GRCm38)	missense	possibly damaging	0.77
R6838:Map4k4	UTSW	1	39,976,722 (GRCm38)	missense	probably damaging	1.00
R6927:Map4k4	UTSW	1	40,011,682 (GRCm38)	missense	probably benign	0.00
R7008:Map4k4	UTSW	1	39,988,971 (GRCm38)	missense	probably benign	0.44
R7164:Map4k4	UTSW	1	39,973,972 (GRCm38)	missense	possibly damaging	0.74
R7195:Map4k4	UTSW	1	40,019,669 (GRCm38)	missense	possibly damaging	0.93
R7589:Map4k4	UTSW	1	40,021,091 (GRCm38)	nonsense	probably null
R7816:Map4k4	UTSW	1	40,014,208 (GRCm38)	missense	possibly damaging	0.53
R7869:Map4k4	UTSW	1	39,974,044 (GRCm38)	missense	unknown
R8013:Map4k4	UTSW	1	39,962,212 (GRCm38)	missense	unknown
R8145:Map4k4	UTSW	1	40,000,534 (GRCm38)	missense
R8154:Map4k4	UTSW	1	40,021,142 (GRCm38)	nonsense	probably null
R8254:Map4k4	UTSW	1	40,006,675 (GRCm38)	missense	probably damaging	0.99
R8266:Map4k4	UTSW	1	40,011,653 (GRCm38)	missense	possibly damaging	0.53
R8375:Map4k4	UTSW	1	40,024,641 (GRCm38)	missense	possibly damaging	0.73
R8487:Map4k4	UTSW	1	39,988,976 (GRCm38)	missense	probably damaging	1.00
R8699:Map4k4	UTSW	1	39,976,750 (GRCm38)	missense	unknown
R8726:Map4k4	UTSW	1	40,003,982 (GRCm38)	missense	possibly damaging	0.95
R8907:Map4k4	UTSW	1	40,019,610 (GRCm38)	missense	probably damaging	0.97
R8956:Map4k4	UTSW	1	40,000,680 (GRCm38)	missense	probably benign	0.11
R8963:Map4k4	UTSW	1	40,000,580 (GRCm38)	missense	probably damaging	1.00
R9091:Map4k4	UTSW	1	40,003,763 (GRCm38)	missense	probably benign	0.02
R9234:Map4k4	UTSW	1	39,990,101 (GRCm38)	missense	unknown
R9270:Map4k4	UTSW	1	40,003,763 (GRCm38)	missense	probably benign	0.02
R9438:Map4k4	UTSW	1	40,006,792 (GRCm38)	missense	probably damaging	0.99
R9689:Map4k4	UTSW	1	40,019,562 (GRCm38)	missense	possibly damaging	0.95
R9771:Map4k4	UTSW	1	39,986,717 (GRCm38)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- CGTTGTTCTATTATGAGCATGCAC -3'
(R):5'- TGTGGCCAAAGATGACATCAC -3'

Sequencing Primer
(F):5'- CTATTATGAGCATGCACTTAAAAGC -3'
(R):5'- TATCTCAACAACTCAAGTCGCCTGG -3'

Posted On

2019-09-13