Incidental Mutation 'R7353:Mcm10'
ID 570709
Institutional Source Beutler Lab
Gene Symbol Mcm10
Ensembl Gene ENSMUSG00000026669
Gene Name minichromosome maintenance 10 replication initiation factor
Synonyms C330019M07Rik, 2410041F14Rik
MMRRC Submission 045439-MU
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R7353 (G1)
Quality Score 225.009
Status Not validated
Chromosome 2
Chromosomal Location 4995535-5017602 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to A at 5011920 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Proline to Serine at position 180 (P180S)
Ref Sequence ENSEMBL: ENSMUSP00000027980 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000027980] [ENSMUST00000102985]
AlphaFold Q0VBD2
Predicted Effect possibly damaging
Transcript: ENSMUST00000027980
AA Change: P180S

PolyPhen 2 Score 0.538 (Sensitivity: 0.88; Specificity: 0.90)
SMART Domains Protein: ENSMUSP00000027980
Gene: ENSMUSG00000026669
AA Change: P180S

DomainStartEndE-ValueType
coiled coil region 102 138 N/A INTRINSIC
low complexity region 218 228 N/A INTRINSIC
Pfam:zf-primase 398 443 2e-21 PFAM
low complexity region 480 493 N/A INTRINSIC
Mcm10 538 883 2.27e-184 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000102985
AA Change: P180S

PolyPhen 2 Score 0.538 (Sensitivity: 0.88; Specificity: 0.90)
SMART Domains Protein: ENSMUSP00000100050
Gene: ENSMUSG00000026669
AA Change: P180S

DomainStartEndE-ValueType
coiled coil region 102 138 N/A INTRINSIC
low complexity region 218 228 N/A INTRINSIC
Pfam:zf-primase 398 443 3.7e-21 PFAM
low complexity region 480 493 N/A INTRINSIC
Mcm10 538 883 2.27e-184 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.9%
  • 20x: 99.6%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit reduced embryonic cell proliferation and early embryonic letahlity. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 67 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
3110070M22Rik C A 13: 119,624,714 (GRCm39) A11S unknown Het
Aadacl4 G A 4: 144,344,490 (GRCm39) V89I probably damaging Het
Abcc9 T A 6: 142,546,731 (GRCm39) I1369F probably damaging Het
Adgrf3 T A 5: 30,403,495 (GRCm39) I427F probably damaging Het
Alox12e T C 11: 70,212,261 (GRCm39) Y139C probably damaging Het
Arhgef2 T A 3: 88,542,993 (GRCm39) V397E possibly damaging Het
Arhgef28 T A 13: 98,211,710 (GRCm39) Y91F probably damaging Het
Bcar3 A G 3: 122,306,341 (GRCm39) T454A probably benign Het
Bicc1 G T 10: 70,783,730 (GRCm39) T469K probably benign Het
Boc C T 16: 44,306,100 (GRCm39) V1070M unknown Het
Ccdc88a T A 11: 29,413,368 (GRCm39) N635K probably benign Het
Ccr2 T C 9: 123,906,793 (GRCm39) S358P probably damaging Het
Ccser2 T C 14: 36,663,100 (GRCm39) Q28R possibly damaging Het
Cenpf T A 1: 189,386,335 (GRCm39) K1982* probably null Het
Csn1s2a A T 5: 87,933,161 (GRCm39) I137F possibly damaging Het
Cttnbp2 A T 6: 18,375,943 (GRCm39) I1532K possibly damaging Het
Dnajc13 C A 9: 104,107,230 (GRCm39) R304L possibly damaging Het
Dop1a T A 9: 86,394,912 (GRCm39) M664K probably damaging Het
Emilin3 T A 2: 160,750,741 (GRCm39) E336V probably damaging Het
Eml5 A G 12: 98,791,683 (GRCm39) Y63H Het
Fbxo41 G T 6: 85,456,958 (GRCm39) R404S possibly damaging Het
Gpr155 A T 2: 73,197,835 (GRCm39) Y456* probably null Het
Gpr156 T A 16: 37,812,523 (GRCm39) N286K probably damaging Het
Kcna5 A G 6: 126,511,808 (GRCm39) S107P probably benign Het
Kcne2 A T 16: 92,093,710 (GRCm39) H79L possibly damaging Het
Kcnh4 T A 11: 100,648,025 (GRCm39) M113L probably benign Het
Lad1 T A 1: 135,755,513 (GRCm39) L263Q probably damaging Het
Lctl G A 9: 64,034,249 (GRCm39) G296D probably damaging Het
Lmtk3 A T 7: 45,437,424 (GRCm39) I205F possibly damaging Het
Magel2 G T 7: 62,029,079 (GRCm39) R661L unknown Het
Mia3 G T 1: 183,108,247 (GRCm39) A446D Het
N4bp2 G A 5: 65,963,714 (GRCm39) V588M probably benign Het
Naip6 C T 13: 100,436,259 (GRCm39) V755M probably benign Het
Neurl1a T C 19: 47,229,099 (GRCm39) V213A probably damaging Het
Nrdc A G 4: 108,896,946 (GRCm39) T522A probably damaging Het
Ntng1 T C 3: 110,042,763 (GRCm39) Q21R probably damaging Het
Nup160 T C 2: 90,534,296 (GRCm39) L707S probably damaging Het
Oas2 A T 5: 120,876,587 (GRCm39) V452D probably damaging Het
Or10a3n A G 7: 108,493,429 (GRCm39) F67L probably damaging Het
Or1j1 A G 2: 36,702,915 (GRCm39) L63P probably damaging Het
Or1j4 A T 2: 36,740,081 (GRCm39) M8L probably benign Het
Or1n1 G A 2: 36,749,680 (GRCm39) R227* probably null Het
Or52s1 A C 7: 102,861,516 (GRCm39) T150P probably damaging Het
Plekhg1 A T 10: 3,914,327 (GRCm39) T1405S Het
Pnrc1 G A 4: 33,248,300 (GRCm39) P33L probably damaging Het
Prkag2 A T 5: 25,085,684 (GRCm39) V312E possibly damaging Het
Rps17 T A 7: 80,994,093 (GRCm39) E76V possibly damaging Het
Rsph10b G A 5: 143,904,038 (GRCm39) G672S possibly damaging Het
Slc2a13 T C 15: 91,205,807 (GRCm39) N460S probably benign Het
Slco2b1 A T 7: 99,339,764 (GRCm39) C56S possibly damaging Het
Speer1f G A 5: 11,466,403 (GRCm39) D7N possibly damaging Het
Spn T C 7: 126,736,178 (GRCm39) T110A probably benign Het
Sult2a8 T C 7: 14,147,640 (GRCm39) N217S possibly damaging Het
Tbx2 C A 11: 85,724,315 (GRCm39) T128N probably damaging Het
Tecpr2 A T 12: 110,934,278 (GRCm39) M1313L probably benign Het
Tmc2 G A 2: 130,038,497 (GRCm39) probably null Het
Tstd1 G T 1: 171,247,523 (GRCm39) A69S probably damaging Het
Txnrd3 A G 6: 89,638,567 (GRCm39) D252G probably benign Het
Ulk2 T C 11: 61,710,174 (GRCm39) N345D probably damaging Het
Unc13c T C 9: 73,481,355 (GRCm39) D1694G probably benign Het
Vill T C 9: 118,894,561 (GRCm39) V406A probably damaging Het
Vmn2r115 T C 17: 23,564,887 (GRCm39) V258A possibly damaging Het
Vmn2r82 T A 10: 79,232,452 (GRCm39) M817K probably benign Het
Xpnpep3 T C 15: 81,315,088 (GRCm39) S263P probably benign Het
Zfp980 A G 4: 145,428,714 (GRCm39) D481G probably benign Het
Zmpste24 A G 4: 120,952,778 (GRCm39) S81P probably damaging Het
Znrf4 A G 17: 56,819,169 (GRCm39) V46A probably benign Het
Other mutations in Mcm10
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01529:Mcm10 APN 2 5,013,439 (GRCm39) missense probably benign 0.00
IGL02028:Mcm10 APN 2 5,013,511 (GRCm39) missense possibly damaging 0.95
IGL02672:Mcm10 APN 2 5,006,092 (GRCm39) missense probably benign 0.00
IGL03352:Mcm10 APN 2 4,999,407 (GRCm39) missense probably damaging 1.00
R0055:Mcm10 UTSW 2 4,996,218 (GRCm39) missense probably damaging 1.00
R0055:Mcm10 UTSW 2 4,996,218 (GRCm39) missense probably damaging 1.00
R0320:Mcm10 UTSW 2 5,008,897 (GRCm39) missense probably benign
R0379:Mcm10 UTSW 2 5,013,434 (GRCm39) missense probably benign 0.05
R0385:Mcm10 UTSW 2 5,008,965 (GRCm39) missense possibly damaging 0.82
R0519:Mcm10 UTSW 2 5,013,356 (GRCm39) missense probably benign
R1537:Mcm10 UTSW 2 5,003,591 (GRCm39) missense possibly damaging 0.77
R1597:Mcm10 UTSW 2 5,003,563 (GRCm39) missense probably damaging 1.00
R1727:Mcm10 UTSW 2 5,011,336 (GRCm39) missense probably benign 0.10
R1758:Mcm10 UTSW 2 5,008,861 (GRCm39) missense probably damaging 1.00
R1997:Mcm10 UTSW 2 4,998,571 (GRCm39) missense probably damaging 1.00
R3618:Mcm10 UTSW 2 5,001,913 (GRCm39) critical splice donor site probably null
R4005:Mcm10 UTSW 2 5,005,814 (GRCm39) missense probably damaging 1.00
R4870:Mcm10 UTSW 2 5,008,970 (GRCm39) missense probably damaging 1.00
R5302:Mcm10 UTSW 2 5,012,181 (GRCm39) missense probably benign 0.12
R5488:Mcm10 UTSW 2 4,996,929 (GRCm39) missense probably damaging 1.00
R6921:Mcm10 UTSW 2 5,005,746 (GRCm39) missense probably benign 0.00
R7259:Mcm10 UTSW 2 5,011,328 (GRCm39) missense probably benign 0.02
R7489:Mcm10 UTSW 2 5,006,112 (GRCm39) missense probably damaging 1.00
R7744:Mcm10 UTSW 2 4,996,253 (GRCm39) missense probably damaging 1.00
R7903:Mcm10 UTSW 2 5,000,613 (GRCm39) missense probably benign 0.00
R9021:Mcm10 UTSW 2 4,997,782 (GRCm39) missense probably benign 0.03
R9072:Mcm10 UTSW 2 5,013,414 (GRCm39) missense possibly damaging 0.63
R9073:Mcm10 UTSW 2 5,013,414 (GRCm39) missense possibly damaging 0.63
R9135:Mcm10 UTSW 2 5,011,372 (GRCm39) missense probably benign 0.01
X0020:Mcm10 UTSW 2 5,011,959 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- AGCTCATTTGCCTGCAACTC -3'
(R):5'- CACCTGGTAATGTGACTGTAATTG -3'

Sequencing Primer
(F):5'- ATTTGCCTGCAACTCTGTCACAAAC -3'
(R):5'- GGTAATGTGACTGTAATTGTAGCAG -3'
Posted On 2019-09-13