Mutagenetix > Incidental Mutation

Incidental Mutation 'R7354:Vmn2r109'

570841

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r109

Ensembl Gene

ENSMUSG00000090572

Gene Name

vomeronasal 2, receptor 109

Synonyms

EG627814

MMRRC Submission

045440-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.091) question?

Stock #

R7354 (G1)

Quality Score

200.009

Status

Validated

Chromosome

Chromosomal Location

20540517-20564756 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 20540781 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 771 (D771E)

Ref Sequence

ENSEMBL: ENSMUSP00000132641 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167093]

AlphaFold

K7N747

Predicted Effect

probably damaging
Transcript: ENSMUST00000167093
AA Change: D771E

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000132641
Gene: ENSMUSG00000090572
AA Change: D771E

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	83	467	1.4e-35	PFAM
Pfam:NCD3G	510	563	3.1e-21	PFAM
Pfam:7tm_3	596	831	7.4e-52	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (82/82)

Allele List at MGI

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700014D04Rik	A	T	13: 59,741,834	L724*	probably null	Het
5430419D17Rik	G	A	7: 131,256,729	C1042Y	possibly damaging	Het
5430419D17Rik	G	T	7: 131,272,033	C1696F	unknown	Het
Ago3	T	A	4: 126,417,306	Q38L	possibly damaging	Het
Apon	T	A	10: 128,254,738	I95N	probably benign	Het
Arid1a	G	T	4: 133,693,947	P464Q	unknown	Het
Arid4b	A	G	13: 14,164,870	D503G	probably benign	Het
Asxl2	T	C	12: 3,455,637		probably benign	Het
Atp2a1	A	T	7: 126,448,856	V594D	probably damaging	Het
Begain	A	G	12: 109,033,289	F519L	possibly damaging	Het
Bora	A	G	14: 99,047,358	T15A	probably damaging	Het
Btbd7	A	T	12: 102,838,205	M192K	probably benign	Het
Ccdc125	T	G	13: 100,677,874		probably null	Het
Cfap74	G	T	4: 155,465,347	V146L	unknown	Het
Crisp1	G	A	17: 40,319,180		probably benign	Het
Ctbp1	A	T	5: 33,250,388	H292Q	possibly damaging	Het
Defb5	T	A	8: 19,250,734	M34K	probably benign	Het
Dnajc27	A	G	12: 4,096,249	I93V	probably benign	Het
Dqx1	T	A	6: 83,060,976	Y448*	probably null	Het
Dynap	A	T	18: 70,241,300	C52S	possibly damaging	Het
Ehd4	C	A	2: 120,102,132	R271L	probably damaging	Het
Ercc2	T	G	7: 19,393,654	I619S	possibly damaging	Het
Etf1	A	G	18: 34,905,987	I409T	probably damaging	Het
Fam117a	A	G	11: 95,380,703	D367G	probably damaging	Het
Fancd2	A	G	6: 113,595,946	D1447G	unknown	Het
Fbxw9	T	C	8: 85,062,196	S192P	probably damaging	Het
Frzb	A	T	2: 80,446,809	L11Q	probably damaging	Het
Gm14496	T	C	2: 182,000,686	S717P	probably damaging	Het
Gm3250	A	G	10: 77,782,533		probably benign	Het
Gm4353	C	T	7: 116,083,911	R145Q	probably benign	Het
Gm765	T	A	6: 98,238,281	D127V	probably damaging	Het
Gpr12	A	T	5: 146,583,962	V50D	probably damaging	Het
Hes1	T	C	16: 30,065,928		probably null	Het
Hmcn1	A	T	1: 150,806,445	C451*	probably null	Het
Iars	T	C	13: 49,704,320	V347A	probably benign	Het
Igfn1	A	G	1: 135,976,032	S323P	possibly damaging	Het
Itga6	G	A	2: 71,820,230	A207T	probably damaging	Het
Lgi4	T	A	7: 31,060,622	L81H	probably damaging	Het
Lrp1	T	C	10: 127,571,408	E1888G	probably damaging	Het
Man2a1	C	T	17: 64,752,544	T1142M	probably damaging	Het
Mgam	A	T	6: 40,744,798	Y350F	probably damaging	Het
Miga1	T	C	3: 152,290,500	D351G	probably damaging	Het
Mro	A	G	18: 73,873,314	T111A	probably benign	Het
Mtrr	A	T	13: 68,566,207	V471E	probably damaging	Het
Myh3	C	A	11: 67,096,882	L1394I	probably damaging	Het
Myocd	A	G	11: 65,187,493	V492A	probably benign	Het
Nbeal2	A	G	9: 110,629,179	F2115S	probably damaging	Het
Nlrp1b	A	T	11: 71,181,550	M489K	possibly damaging	Het
Olfr1228	T	C	2: 89,248,687		probably null	Het
Olfr128	A	G	17: 37,924,393	I276V	probably benign	Het
Olfr1294	C	T	2: 111,537,564	A242T	possibly damaging	Het
Olfr376	A	T	11: 73,375,375	I212F	probably benign	Het
Olfr536	A	C	7: 140,504,186	I91R	probably damaging	Het
Orc2	A	G	1: 58,469,747	S462P	possibly damaging	Het
Pcdh9	T	C	14: 93,888,270	T155A	probably benign	Het
Pcdhb16	G	A	18: 37,478,124	V46I	possibly damaging	Het
Pcdhb22	A	G	18: 37,520,258	D336G	probably damaging	Het
Pde4dip	T	A	3: 97,719,330	R1297S	probably damaging	Het
Plin4	A	G	17: 56,104,427	M868T	probably benign	Het
Plxnb2	A	G	15: 89,165,725	M531T	possibly damaging	Het
Poc5	T	C	13: 96,394,525	V77A	probably benign	Het
Recql5	G	A	11: 115,928,201	R180C	probably damaging	Het
Rho	C	A	6: 115,935,503	Y268*	probably null	Het
Riok1	A	T	13: 38,047,312	H182L	probably benign	Het
Rnf207	T	C	4: 152,314,091	D273G	probably damaging	Het
Serpinb11	A	G	1: 107,377,533	Y213C	probably benign	Het
Slc16a10	T	C	10: 40,076,955	Y181C	probably damaging	Het
Slc22a15	G	A	3: 101,864,581	H401Y	probably benign	Het
Slc5a5	C	A	8: 70,889,603	R268L	probably damaging	Het
Slc9a8	T	C	2: 167,474,131	F576S	possibly damaging	Het
Slx4	T	C	16: 3,987,099	E617G	probably benign	Het
Top1	T	C	2: 160,704,958	I386T	probably damaging	Het
Tor1aip1	G	T	1: 156,036,113	D41E	probably damaging	Het
Tsc22d4	G	A	5: 137,768,109	R479Q	probably benign	Het
Unc79	C	T	12: 103,142,702	T2191I	possibly damaging	Het
Vmn2r82	A	C	10: 79,356,630	M14L	probably benign	Het
Zc3h7a	C	T	16: 11,148,514	S583N	probably damaging	Het
Zfp518b	G	A	5: 38,682,779		probably benign	Het
Zswim4	A	G	8: 84,228,849	W314R	probably damaging	Het

Other mutations in Vmn2r109

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01313:Vmn2r109	APN	17	20550157	missense	probably damaging	1.00
IGL01383:Vmn2r109	APN	17	20541121	missense	possibly damaging	0.89
IGL01469:Vmn2r109	APN	17	20541409	missense	probably damaging	1.00
IGL01762:Vmn2r109	APN	17	20554392	missense	probably benign
IGL01864:Vmn2r109	APN	17	20541134	missense	probably benign	0.28
IGL02028:Vmn2r109	APN	17	20541080	missense	probably benign	0.28
IGL02074:Vmn2r109	APN	17	20554341	missense	probably benign	0.05
IGL02162:Vmn2r109	APN	17	20554160	missense	probably benign	0.01
IGL02474:Vmn2r109	APN	17	20540888	missense	probably benign
IGL02490:Vmn2r109	APN	17	20540984	missense	possibly damaging	0.78
IGL02604:Vmn2r109	APN	17	20540701	missense	probably damaging	1.00
IGL02669:Vmn2r109	APN	17	20554256	missense	possibly damaging	0.64
IGL02705:Vmn2r109	APN	17	20553800	missense	probably benign
IGL02745:Vmn2r109	APN	17	20541250	missense	probably damaging	0.99
PIT4142001:Vmn2r109	UTSW	17	20554577	critical splice acceptor site	probably null
R0389:Vmn2r109	UTSW	17	20541074	missense	probably damaging	1.00
R0470:Vmn2r109	UTSW	17	20552886	missense	probably benign	0.06
R0570:Vmn2r109	UTSW	17	20540675	missense	probably damaging	0.99
R0855:Vmn2r109	UTSW	17	20541408	nonsense	probably null
R0882:Vmn2r109	UTSW	17	20554580	splice site	probably benign
R1241:Vmn2r109	UTSW	17	20555241	missense	possibly damaging	0.86
R1587:Vmn2r109	UTSW	17	20540740	missense	probably damaging	1.00
R1931:Vmn2r109	UTSW	17	20553810	nonsense	probably null
R1957:Vmn2r109	UTSW	17	20564707	missense	probably benign	0.11
R1962:Vmn2r109	UTSW	17	20553923	missense	probably damaging	0.99
R2020:Vmn2r109	UTSW	17	20541186	nonsense	probably null
R2073:Vmn2r109	UTSW	17	20564712	missense	probably benign	0.00
R2436:Vmn2r109	UTSW	17	20554536	missense	probably damaging	0.99
R3123:Vmn2r109	UTSW	17	20540986	missense	probably damaging	1.00
R3839:Vmn2r109	UTSW	17	20554442	missense	probably damaging	1.00
R4019:Vmn2r109	UTSW	17	20553812	missense	probably benign
R4428:Vmn2r109	UTSW	17	20553024	missense	probably benign
R4584:Vmn2r109	UTSW	17	20554558	nonsense	probably null
R4652:Vmn2r109	UTSW	17	20541394	missense	probably damaging	1.00
R4708:Vmn2r109	UTSW	17	20541343	missense	probably damaging	0.97
R4823:Vmn2r109	UTSW	17	20553891	missense	probably damaging	1.00
R4831:Vmn2r109	UTSW	17	20541232	missense	probably benign	0.01
R4907:Vmn2r109	UTSW	17	20550086	missense	probably damaging	1.00
R5011:Vmn2r109	UTSW	17	20555189	missense	probably damaging	1.00
R5296:Vmn2r109	UTSW	17	20554341	missense	possibly damaging	0.90
R5600:Vmn2r109	UTSW	17	20540927	missense	probably damaging	1.00
R5602:Vmn2r109	UTSW	17	20540671	missense	possibly damaging	0.94
R5652:Vmn2r109	UTSW	17	20540519	makesense	probably null
R5702:Vmn2r109	UTSW	17	20554145	missense	probably benign	0.42
R5706:Vmn2r109	UTSW	17	20554305	missense	probably benign	0.16
R5714:Vmn2r109	UTSW	17	20552859	missense	probably damaging	1.00
R5832:Vmn2r109	UTSW	17	20541056	missense	probably benign	0.10
R6008:Vmn2r109	UTSW	17	20540719	missense	probably damaging	1.00
R6334:Vmn2r109	UTSW	17	20541178	missense	probably benign	0.18
R6377:Vmn2r109	UTSW	17	20564534	critical splice donor site	probably null
R6738:Vmn2r109	UTSW	17	20554523	missense	possibly damaging	0.52
R6857:Vmn2r109	UTSW	17	20540670	missense	probably benign	0.45
R6953:Vmn2r109	UTSW	17	20540711	missense	possibly damaging	0.95
R7108:Vmn2r109	UTSW	17	20564744	missense	probably benign	0.03
R7229:Vmn2r109	UTSW	17	20540963	missense	possibly damaging	0.80
R7238:Vmn2r109	UTSW	17	20541074	missense	probably damaging	1.00
R7244:Vmn2r109	UTSW	17	20540683	missense	possibly damaging	0.70
R7292:Vmn2r109	UTSW	17	20541438	missense	probably benign	0.05
R7357:Vmn2r109	UTSW	17	20541274	missense	probably damaging	1.00
R7522:Vmn2r109	UTSW	17	20554403	missense	probably benign	0.11
R7596:Vmn2r109	UTSW	17	20540680	missense	probably damaging	0.98
R7728:Vmn2r109	UTSW	17	20552855	missense	probably damaging	0.99
R7859:Vmn2r109	UTSW	17	20541174	missense	probably damaging	1.00
R7871:Vmn2r109	UTSW	17	20540520	missense	probably benign	0.08
R8113:Vmn2r109	UTSW	17	20554467	missense	probably benign	0.01
R8153:Vmn2r109	UTSW	17	20564707	missense	probably benign	0.11
R8977:Vmn2r109	UTSW	17	20554269	missense	possibly damaging	0.96
R9687:Vmn2r109	UTSW	17	20555070	missense
Z1176:Vmn2r109	UTSW	17	20552994	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CACTTAGGAGCAAATATGAAGCC -3'
(R):5'- CCTTTCCAGGGAGAATGGTGAG -3'

Sequencing Primer
(F):5'- CAAGGAGGGCTGCACTG -3'
(R):5'- GGTTAATGACCTCAAGAGCTCC -3'

Posted On

2019-09-13