Mutagenetix > Incidental Mutations

Incidental Mutation 'R7360:4932415D10Rik'

571273

Institutional Source

Beutler Lab

Gene Symbol

4932415D10Rik

Ensembl Gene

ENSMUSG00000044581

Gene Name

RIKEN cDNA 4932415D10 gene

Synonyms

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.075) question?

Stock #

R7360 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

82282116-82316582 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 82296507 bp

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 223 (D223G)

Ref Sequence

ENSEMBL: ENSMUSP00000151425 (fasta)

Predicted Effect

unknown
Transcript: ENSMUST00000217661
AA Change: D223G

Meta Mutation Damage Score

0.0698

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (54/54)

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4922502D21Rik	C	T	6: 129,326,747	R85H	probably benign	Het
Acot11	G	C	4: 106,749,351	P534A	possibly damaging	Het
Arhgef26	T	A	3: 62,448,205	Y733N	possibly damaging	Het
Aste1	G	T	9: 105,397,636	M358I	probably damaging	Het
B4galnt3	C	T	6: 120,232,979	W61*	probably null	Het
Brd9	C	T	13: 73,944,823	R311W	probably benign	Het
Cdkn1c	T	C	7: 143,460,694	D5G	possibly damaging	Het
Cerk	A	G	15: 86,159,126	F158S	probably damaging	Het
Cnot4	T	A	6: 35,065,006	E235V	probably damaging	Het
Crmp1	T	C	5: 37,276,280	V275A	possibly damaging	Het
Dcbld2	C	A	16: 58,465,320		probably null	Het
Dip2a	T	C	10: 76,278,560	R1029G	probably damaging	Het
Dnaaf1	A	G	8: 119,577,351	T43A	probably benign	Het
Eaf2	C	T	16: 36,828,152	S2N	probably benign	Het
Eif2b4	T	C	5: 31,191,375	D164G	probably benign	Het
Fpgs	T	C	2: 32,693,993	Y45C	possibly damaging	Het
Gm1527	C	T	3: 28,914,542	Q248*	probably null	Het
Gm29666	C	T	15: 84,914,268	A31T	unknown	Het
Gmip	C	A	8: 69,811,242	A112D	probably damaging	Het
Hibadh	C	T	6: 52,640,212	G13S	probably benign	Het
Hmcn1	A	T	1: 150,618,846	V4164D	probably damaging	Het
Kif15	A	T	9: 122,991,137	N580I	probably benign	Het
Krt25	G	A	11: 99,317,406	T332M	probably benign	Het
Krt88	G	T	15: 101,447,762		probably benign	Het
Lrrk2	T	C	15: 91,731,655		probably null	Het
Mapkapk5	A	G	5: 121,537,106		probably benign	Het
Myh7b	C	T	2: 155,632,540	S1725L	probably benign	Het
Nckap1l	T	G	15: 103,476,099		probably null	Het
Nphp3	A	G	9: 104,016,078		probably null	Het
Obscn	A	C	11: 59,082,359	V1996G	probably damaging	Het
Olfr1196	A	T	2: 88,700,987	V114E	probably damaging	Het
Olfr761	T	A	17: 37,953,009	N5I	probably damaging	Het
Parp8	T	C	13: 116,895,771	T289A	probably benign	Het
Pcsk5	T	C	19: 17,515,213	K932R	probably benign	Het
Pde4dip	T	C	3: 97,718,316	D1322G	probably benign	Het
Peli3	A	T	19: 4,935,075	M136K	possibly damaging	Het
Pgm5	A	G	19: 24,834,817	I117T	probably damaging	Het
Ppm1g	T	C	5: 31,203,277	D478G	probably damaging	Het
Ppp2r5c	A	G	12: 110,574,838	T474A	probably benign	Het
Ptger3	T	C	3: 157,567,127	V37A	probably benign	Het
Ptprz1	T	C	6: 23,000,907	S999P	probably damaging	Het
Pygl	C	T	12: 70,227,532	G18S	probably benign	Het
Rest	T	C	5: 77,281,129	V465A	probably benign	Het
Sart1	T	C	19: 5,383,203	D422G	probably damaging	Het
Sgk1	A	G	10: 21,994,073	M4V	probably benign	Het
Slc33a1	A	G	3: 63,947,654	V395A	possibly damaging	Het
Slc38a11	A	T	2: 65,353,795	S171T	possibly damaging	Het
Slc4a2	T	A	5: 24,429,715	S76T	probably benign	Het
Ssc4d	T	A	5: 135,966,111	S184C	probably damaging	Het
Tspoap1	A	G	11: 87,778,521	Y1540C	probably benign	Het
Ube3a	T	A	7: 59,276,635	L408Q	probably damaging	Het
Usp43	C	A	11: 67,876,329		probably null	Het
Zan	A	C	5: 137,386,970	V5067G	unknown	Het
Zfp180	C	A	7: 24,105,490	L445I	probably damaging	Het

Other mutations in 4932415D10Rik

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00090:4932415D10Rik	APN	10	82283752	missense	probably benign	0.06
IGL01457:4932415D10Rik	APN	10	82284734	missense	probably damaging	1.00
IGL01540:4932415D10Rik	APN	10	82284182	missense	possibly damaging	0.87
IGL02693:4932415D10Rik	APN	10	82285258	missense	probably benign	0.06
IGL02867:4932415D10Rik	APN	10	82283820	missense	probably damaging	0.96
IGL02889:4932415D10Rik	APN	10	82283820	missense	probably damaging	0.96
IGL03080:4932415D10Rik	APN	10	82283982	missense	probably damaging	0.99
IGL03120:4932415D10Rik	APN	10	82285035	missense	possibly damaging	0.90
IGL03351:4932415D10Rik	APN	10	82283567	utr 3 prime	probably benign
FR4449:4932415D10Rik	UTSW	10	82285469	frame shift	probably null
FR4548:4932415D10Rik	UTSW	10	82290996	small insertion	probably benign
FR4737:4932415D10Rik	UTSW	10	82285469	small deletion	probably benign
PIT4480001:4932415D10Rik	UTSW	10	82283752	missense	probably benign	0.06
R0102:4932415D10Rik	UTSW	10	82283556	missense	probably damaging	1.00
R0312:4932415D10Rik	UTSW	10	82284369	missense	probably damaging	1.00
R1303:4932415D10Rik	UTSW	10	82284556	missense	possibly damaging	0.94
R2039:4932415D10Rik	UTSW	10	82284676	missense	probably damaging	1.00
R2356:4932415D10Rik	UTSW	10	82283955	missense	possibly damaging	0.94
R4740:4932415D10Rik	UTSW	10	82283647	missense	possibly damaging	0.50
R4857:4932415D10Rik	UTSW	10	82283848	missense	possibly damaging	0.61
R5017:4932415D10Rik	UTSW	10	82296676	missense	unknown
R5095:4932415D10Rik	UTSW	10	82283667	missense	probably damaging	1.00
R5209:4932415D10Rik	UTSW	10	82283818	missense	possibly damaging	0.84
R5388:4932415D10Rik	UTSW	10	82283727	missense	probably damaging	0.99
R5642:4932415D10Rik	UTSW	10	82284483	missense	probably damaging	1.00
R5646:4932415D10Rik	UTSW	10	82283776	missense	probably damaging	0.99
R6188:4932415D10Rik	UTSW	10	82285257	missense	probably damaging	0.96
R6215:4932415D10Rik	UTSW	10	82291112	missense	probably benign	0.07
R6252:4932415D10Rik	UTSW	10	82283754	missense	probably benign	0.30
R6275:4932415D10Rik	UTSW	10	82285368	missense	probably damaging	1.00
R6303:4932415D10Rik	UTSW	10	82290368	missense	possibly damaging	0.79
R6304:4932415D10Rik	UTSW	10	82290368	missense	possibly damaging	0.79
R6313:4932415D10Rik	UTSW	10	82293636	missense	probably benign	0.00
R6323:4932415D10Rik	UTSW	10	82283082	missense	probably benign	0.27
R6374:4932415D10Rik	UTSW	10	82288897	unclassified	probably benign
R6407:4932415D10Rik	UTSW	10	82293811	missense	probably benign	0.16
R6468:4932415D10Rik	UTSW	10	82295316	missense	probably benign	0.01
R6490:4932415D10Rik	UTSW	10	82289304	missense	possibly damaging	0.90
R6605:4932415D10Rik	UTSW	10	82296037	missense	probably benign	0.27
R6614:4932415D10Rik	UTSW	10	82291648	missense	probably benign	0.31
R6626:4932415D10Rik	UTSW	10	82292833	missense	probably benign	0.03
R6630:4932415D10Rik	UTSW	10	82287072	missense	possibly damaging	0.81
R6646:4932415D10Rik	UTSW	10	82296830	missense	unknown
R6723:4932415D10Rik	UTSW	10	82289823	missense	possibly damaging	0.50
R6751:4932415D10Rik	UTSW	10	82283497	missense	probably benign	0.06
R6850:4932415D10Rik	UTSW	10	82293054	missense	possibly damaging	0.68
R6944:4932415D10Rik	UTSW	10	82296222	missense	probably benign	0.03
R6957:4932415D10Rik	UTSW	10	82293786	missense	probably benign	0.03
R6988:4932415D10Rik	UTSW	10	82291899	missense	possibly damaging	0.79
R7069:4932415D10Rik	UTSW	10	82289943	missense	probably damaging	0.99
R7164:4932415D10Rik	UTSW	10	82286229	missense	probably damaging	1.00
R7175:4932415D10Rik	UTSW	10	82286749	missense	probably damaging	1.00
R7201:4932415D10Rik	UTSW	10	82291627	missense	probably benign	0.03
R7203:4932415D10Rik	UTSW	10	82293414	missense	probably benign	0.00
R7205:4932415D10Rik	UTSW	10	82289327	missense	probably benign	0.35
R7241:4932415D10Rik	UTSW	10	82287042	missense	probably benign	0.01
R7283:4932415D10Rik	UTSW	10	82291297	missense	possibly damaging	0.90
R7305:4932415D10Rik	UTSW	10	82285119	missense	probably benign	0.06
R7358:4932415D10Rik	UTSW	10	82292013	missense	possibly damaging	0.79
R7362:4932415D10Rik	UTSW	10	82292997	missense	possibly damaging	0.79
R7385:4932415D10Rik	UTSW	10	82287737	missense	probably benign	0.03
R7385:4932415D10Rik	UTSW	10	82287895	missense	probably benign	0.05
R7472:4932415D10Rik	UTSW	10	82283587	missense	probably benign	0.03
R7493:4932415D10Rik	UTSW	10	82288964	nonsense	probably null
R7493:4932415D10Rik	UTSW	10	82316430	missense	unknown
R7498:4932415D10Rik	UTSW	10	82291279	missense	probably benign	0.03
R7512:4932415D10Rik	UTSW	10	82292635	missense	probably benign	0.31
R7560:4932415D10Rik	UTSW	10	82284615	missense	probably damaging	1.00
R7591:4932415D10Rik	UTSW	10	82292212	missense	probably benign	0.16
R7636:4932415D10Rik	UTSW	10	82295139	missense	probably benign	0.01
R7640:4932415D10Rik	UTSW	10	82294656	missense	probably damaging	0.99
R7709:4932415D10Rik	UTSW	10	82290532	missense	possibly damaging	0.81
R7790:4932415D10Rik	UTSW	10	82287495	missense	probably benign	0.06
RF017:4932415D10Rik	UTSW	10	82290992	small insertion	probably benign
RF055:4932415D10Rik	UTSW	10	82290993	small insertion	probably benign

Predicted Primers

PCR Primer
(F):5'- TTCTGGAAGACCTGTGACCC -3'
(R):5'- TCTTTCCTGAAAGTACAACTTGGC -3'

Sequencing Primer
(F):5'- AAGAGGCTTCTTCCTGAATTGC -3'
(R):5'- TCCTGAAAGTACAACTTGGCAAATC -3'

Posted On

2019-09-13